FastQCFastQC Report
Fri 27 May 2016
SRR939790_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939790_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2543353
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45910.1805097444200628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33410.13136202485459156No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30910.12153248094149731No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG95400.025.5922072
GTACATG96400.025.4238241
GTATCAA125600.024.9813791
ACATGGG97800.024.2431473
ATCAACG141400.022.0244923
TCAACGC142600.022.0045324
CAACGCA142700.021.923615
TATCAAC143800.021.6564792
AACGCAG145800.021.522856
GAGTACT52500.020.29779812-13
AGTACTT56000.020.12136812-13
CATGGGG44500.020.0862774
GGTATCA91550.019.7825641
ACGCAGA160250.019.3472337
CGCAGAG161450.018.941178
CATGGGA57800.018.6386094
AGAGTAC114350.018.47317910-11
GTACTTT58700.018.31763314-15
GCAGAGT162200.017.6933659
TAAGGTG8200.017.2116225
Produced by FastQC (version 0.11.5)