FastQCFastQC Report
Fri 27 May 2016
SRR939790_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939790_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2543353
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49260.19368133326360912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41550.16336701983562643No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36200.14233179586160474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28460.11189952790666494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG100850.024.8910621
TACATGG101550.024.2479042
ACATGGG103550.022.9956913
GTATCAA117800.022.7999971
GGTATCA88950.022.352791
ATCAACG133750.019.7539633
TCAACGC134800.019.6352834
CAACGCA136350.019.3424975
TATCAAC138750.019.1146162
AACGCAG138600.019.0284946
GAGTACT55500.018.58913212-13
CATGGGA59750.018.4974084
ATCGCCG1202.8744907E-617.9505664-65
CCGCGCA1600.001202659717.7878789
AGTACTT59750.017.74322112-13
CATGGGG47800.017.56464
TACACTG22150.017.1320425
ACGCAGA153600.017.0775997
CGCAGAG155150.016.9069868
GTATAGG8150.016.8851151
Produced by FastQC (version 0.11.5)