##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939790_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2543353 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.368712876270028 31.0 31.0 34.0 30.0 34.0 2 31.602266771462713 31.0 31.0 34.0 30.0 34.0 3 31.650122102594487 31.0 31.0 34.0 30.0 34.0 4 35.46126825493748 37.0 35.0 37.0 33.0 37.0 5 35.192524985717675 37.0 35.0 37.0 32.0 37.0 6 35.21637106606908 37.0 35.0 37.0 32.0 37.0 7 35.119585051701435 37.0 35.0 37.0 32.0 37.0 8 35.080884957770316 37.0 35.0 37.0 32.0 37.0 9 36.664116620854436 39.0 35.0 39.0 32.0 39.0 10-11 36.51946485603847 38.0 35.0 39.0 32.0 39.0 12-13 36.44518279609633 38.0 35.0 39.0 32.0 39.0 14-15 37.48003049517703 39.0 36.0 41.0 32.0 41.0 16-17 37.40231654827309 39.0 36.0 41.0 32.0 41.0 18-19 37.365469126778706 39.0 36.0 41.0 32.0 41.0 20-21 37.27249972772164 39.0 36.0 40.0 31.0 41.0 22-23 37.14606839868473 39.0 36.0 40.0 31.0 41.0 24-25 37.00391982552166 39.0 36.0 40.0 31.0 41.0 26-27 36.714571276578596 39.0 35.5 40.0 30.0 41.0 28-29 36.49491262125234 38.0 35.0 40.0 30.0 41.0 30-31 36.253835193148575 38.0 35.0 40.0 30.0 41.0 32-33 36.28623985738511 38.0 35.0 40.0 30.0 41.0 34-35 36.16173570872781 38.0 35.0 40.0 30.0 41.0 36-37 36.2414126155512 38.0 35.0 40.0 30.0 41.0 38-39 36.17017142331403 38.0 35.0 40.0 30.0 41.0 40-41 36.112143497186594 38.0 35.0 40.0 30.0 41.0 42-43 35.92424232892563 38.0 35.0 40.0 29.0 41.0 44-45 35.72196230723773 38.0 34.5 40.0 28.0 41.0 46-47 35.49429611225811 38.0 34.0 40.0 27.0 41.0 48-49 35.24275257897743 38.0 34.0 40.0 27.0 41.0 50-51 35.050459373905234 38.0 34.0 40.0 26.0 41.0 52-53 34.820071771397835 38.0 33.0 40.0 26.0 41.0 54-55 34.57680314136496 38.0 33.0 40.0 26.0 41.0 56-57 34.22615185544437 37.5 33.0 40.0 24.5 41.0 58-59 33.87821470318906 37.0 32.0 40.0 23.5 41.0 60-61 33.57222237731058 37.0 32.0 39.0 23.0 41.0 62-63 33.29220776667651 36.0 31.5 39.0 23.0 40.5 64-65 32.855175235211156 36.0 31.0 39.0 22.0 40.0 66-67 31.92711825688373 35.0 30.0 38.5 17.0 40.0 68-69 29.976016699215563 34.0 27.0 37.5 10.0 40.0 70-71 29.307952730116504 34.0 26.0 37.0 9.5 39.0 72-73 29.886153042853273 34.0 27.5 36.5 10.5 39.0 74-75 30.00458626859897 34.0 28.0 36.0 12.5 39.0 76-77 29.55166447598898 32.5 28.5 35.0 13.0 38.0 78-79 30.090126301775648 34.0 29.0 36.0 14.5 37.5 80-81 29.89436031883895 34.0 29.0 35.0 12.0 37.0 82-83 29.43510554767663 34.0 29.0 35.0 7.5 37.0 84-85 29.06490329891289 33.5 29.0 35.0 7.0 36.0 86-87 28.678627779942463 33.0 28.5 35.0 3.5 36.0 88-89 28.234372892791527 33.0 27.0 35.0 2.0 35.5 90-91 27.739908695332502 33.0 26.0 35.0 2.0 35.0 92-93 27.244216001475216 32.5 26.0 34.5 2.0 35.0 94-95 26.419663923961792 32.0 25.0 34.0 2.0 35.0 96-97 25.896655713933534 32.0 24.0 34.0 2.0 35.0 98-99 25.23144604779596 32.0 21.5 34.0 2.0 35.0 100-101 23.73753997184032 30.5 10.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.20660417321002456 1101 2 0.202643051857887 1101 3 0.2573782219183407 1101 4 0.216471343244514 1101 5 0.1577117983891796 1101 6 0.16566555892026713 1101 7 0.20845837991648608 1101 8 0.2509009898619041 1101 9 0.3703357766798163 1101 10-11 0.3972912034609948 1101 12-13 0.37967629621920906 1101 14-15 0.3648503982767579 1101 16-17 0.43394269855694034 1101 18-19 0.2373710638148978 1101 20-21 0.4335854038716178 1101 22-23 0.3493969397839436 1101 24-25 0.3086510453306701 1101 26-27 0.18491696889338272 1101 28-29 0.2047528249735251 1101 30-31 0.17258354854160274 1101 32-33 0.03608824718474324 1101 34-35 0.02985384600292207 1101 36-37 0.13268497544692082 1101 38-39 0.09794365509177538 1101 40-41 0.11786315952582527 1101 42-43 -0.0607148828062094 1101 44-45 -0.061520222166159044 1101 46-47 -0.06959729651058666 1101 48-49 0.09055985805569122 1101 50-51 -0.06464824490264931 1101 52-53 -0.08810590954497144 1101 54-55 -0.4422135942134986 1101 56-57 -0.36984236060852993 1101 58-59 -0.1526552699739554 1101 60-61 -0.35457250681061225 1101 62-63 -0.2689585259362488 1101 64-65 -0.461826044927399 1101 66-67 -0.3756422206745178 1101 68-69 -9.173788035163636 1101 70-71 -7.128627511097513 1101 72-73 -1.9905039112351375 1101 74-75 -0.8331829216909554 1101 76-77 -0.5720597883184588 1101 78-79 -0.8996667601472694 1101 80-81 -1.0162299838013418 1101 82-83 -0.7004998720004032 1101 84-85 -0.62240855497501 1101 86-87 -0.771963870013952 1101 88-89 -0.5666296314580315 1101 90-91 -0.993709338558812 1101 92-93 -1.044534837055398 1101 94-95 -0.7922704022497022 1101 96-97 -1.3092841465998895 1101 98-99 -1.287195152284884 1101 100-101 -1.2725042483131332 1102 1 0.21252606746020675 1102 2 0.15374412523167535 1102 3 0.2814558965231946 1102 4 0.2446841705750913 1102 5 0.23879052900390008 1102 6 0.20043666228520607 1102 7 0.3168226808769319 1102 8 0.17007297299882396 1102 9 0.4327305743547001 1102 10-11 0.4166707267918355 1102 12-13 0.304658696054787 1102 14-15 0.36633068727382323 1102 16-17 0.4886751494802368 1102 18-19 0.3641206707642439 1102 20-21 0.38985276040960315 1102 22-23 0.4516401634253313 1102 24-25 0.3179819378716289 1102 26-27 0.38259553378306776 1102 28-29 0.38505041540470586 1102 30-31 0.34792326715159305 1102 32-33 0.14010042023807046 1102 34-35 0.18712283203264235 1102 36-37 0.04002025739801951 1102 38-39 0.40824799028394665 1102 40-41 0.2596126247288879 1102 42-43 0.1955550281180649 1102 44-45 0.21258816413500625 1102 46-47 0.1405862442645116 1102 48-49 0.23371925589382414 1102 50-51 0.19602694878775395 1102 52-53 0.2773555248329913 1102 54-55 -0.002893483249778228 1102 56-57 0.0547079345190582 1102 58-59 -0.06750797785965545 1102 60-61 0.10722841820205531 1102 62-63 0.07189693359931937 1102 64-65 -0.0024692836883914993 1102 66-67 0.22234123162725794 1102 68-69 -4.6254870268750174 1102 70-71 0.2988745767072274 1102 72-73 0.6474223169078819 1102 74-75 0.5039736240936854 1102 76-77 0.25811914865523633 1102 78-79 0.2696237140295068 1102 80-81 0.10327793804447438 1102 82-83 0.22899042900965583 1102 84-85 0.03654979583052054 1102 86-87 -0.1656243262243855 1102 88-89 -0.21075519268475063 1102 90-91 -0.23867771437075191 1102 92-93 -0.17068054574550828 1102 94-95 0.17233517983734714 1102 96-97 -0.5413134485112217 1102 98-99 -0.15666697312229516 1102 100-101 0.041965360691541775 1103 1 0.21650299103233905 1103 2 0.24111968634060688 1103 3 0.18382586848969495 1103 4 0.2288900148867441 1103 5 0.282810878164355 1103 6 0.1494149611960509 1103 7 0.2108052813790806 1103 8 0.15227155435157158 1103 9 0.306140448701413 1103 10-11 0.31230841575460033 1103 12-13 0.30996589405089026 1103 14-15 0.2722912443152623 1103 16-17 0.29547861357804095 1103 18-19 0.3182937264157104 1103 20-21 0.2330042320220258 1103 22-23 0.21449972671139506 1103 24-25 0.253251621309623 1103 26-27 0.14366898564913555 1103 28-29 0.04446226789816876 1103 30-31 0.11885782443884096 1103 32-33 0.0470684860436279 1103 34-35 0.03371433302464766 1103 36-37 0.17599663083466766 1103 38-39 0.2177174899532801 1103 40-41 0.006447077841542637 1103 42-43 -0.15001989272680305 1103 44-45 -0.005786361080510005 1103 46-47 -0.19661147384372413 1103 48-49 -0.2282995218689976 1103 50-51 -0.1185442527900804 1103 52-53 -0.014785072824842871 1103 54-55 -0.15363111836948917 1103 56-57 -0.146319253293683 1103 58-59 -0.16091659391776147 1103 60-61 -0.3256045015542455 1103 62-63 -0.3331642397109178 1103 64-65 -0.3721154119636054 1103 66-67 -0.07913725749000733 1103 68-69 -4.512156556392128 1103 70-71 -0.012737964040837824 1103 72-73 0.3856813136714443 1103 74-75 0.15326166684038256 1103 76-77 -0.12755845439275149 1103 78-79 -0.33551939435303524 1103 80-81 -0.12799009498706226 1103 82-83 -0.20826332683553161 1103 84-85 -0.3086238626289841 1103 86-87 -0.31087209203053945 1103 88-89 -0.26477410798144163 1103 90-91 -0.2700751609227474 1103 92-93 -0.39300924291933015 1103 94-95 -0.35336456031142305 1103 96-97 -0.5699286141078552 1103 98-99 -0.293579820541634 1103 100-101 -0.5047951313870946 1104 1 0.22305898094380083 1104 2 0.17874095717590777 1104 3 0.18989588773401778 1104 4 0.1487781124386487 1104 5 0.27705177792704916 1104 6 0.18792618444938824 1104 7 0.25628534955410487 1104 8 0.18526786946443252 1104 9 0.32512581634184556 1104 10-11 0.2829036410892485 1104 12-13 0.28531015876647814 1104 14-15 0.2059851395838237 1104 16-17 0.27320644870862765 1104 18-19 0.13319185784927612 1104 20-21 0.21787376316609652 1104 22-23 0.17547449329143916 1104 24-25 0.17058116777248955 1104 26-27 0.32599785419499483 1104 28-29 0.28405439611037764 1104 30-31 0.22677695994202196 1104 32-33 0.195335254551658 1104 34-35 0.430574547956283 1104 36-37 0.24063797014313337 1104 38-39 0.4027796063179139 1104 40-41 0.26770649858097784 1104 42-43 0.3241440865253864 1104 44-45 0.2720708816641846 1104 46-47 0.2964215268725212 1104 48-49 0.31749551151930433 1104 50-51 0.4351278923452071 1104 52-53 0.25244377989112365 1104 54-55 0.15430391801442767 1104 56-57 0.36941133183743347 1104 58-59 0.16137416930322246 1104 60-61 0.0844382533585204 1104 62-63 0.1998953239944683 1104 64-65 0.2587890855011281 1104 66-67 0.5805903547443059 1104 68-69 -3.7212808224848466 1104 70-71 0.9465879379969593 1104 72-73 1.3427733549257148 1104 74-75 0.8731183885472866 1104 76-77 0.7344969332018643 1104 78-79 0.43211961590232306 1104 80-81 0.245200878139741 1104 82-83 0.13518916608892795 1104 84-85 0.2591370307165697 1104 86-87 0.2577595874928882 1104 88-89 0.10590651360102399 1104 90-91 0.2010509692642941 1104 92-93 0.08684860036754927 1104 94-95 0.4793069225237865 1104 96-97 0.20786452821141665 1104 98-99 0.5708397582695923 1104 100-101 0.5502646495356807 1105 1 0.1973501676383549 1105 2 0.1868970041427218 1105 3 0.23411716685314232 1105 4 0.18590388104086486 1105 5 0.1481645020568081 1105 6 0.2520885697403372 1105 7 0.09867072441660696 1105 8 0.24717673717429278 1105 9 0.3334179442747356 1105 10-11 0.2782675178163885 1105 12-13 0.3200770274567333 1105 14-15 0.37669997024134005 1105 16-17 0.32439582851926474 1105 18-19 0.18402823831934256 1105 20-21 0.2698613074920644 1105 22-23 0.3974509399558812 1105 24-25 0.35014678558078316 1105 26-27 0.3694225771555466 1105 28-29 0.3917487048351518 1105 30-31 0.253237015703057 1105 32-33 0.25574583306358534 1105 34-35 0.3696040515124963 1105 36-37 0.32852291809181366 1105 38-39 0.42630049502592016 1105 40-41 0.30475772334463613 1105 42-43 0.20983831273446896 1105 44-45 0.36868815779315867 1105 46-47 0.2030755784280487 1105 48-49 0.3477833863975448 1105 50-51 0.4071780087412833 1105 52-53 0.3853849538936416 1105 54-55 0.20215700651390733 1105 56-57 0.24713454203357088 1105 58-59 0.37840498891481644 1105 60-61 0.19185368700996008 1105 62-63 0.2589008280709564 1105 64-65 0.24956774694273776 1105 66-67 0.7507444982558802 1105 68-69 2.1110191766060815 1105 70-71 2.3584268421885497 1105 72-73 1.5150304598416184 1105 74-75 0.9353046628308519 1105 76-77 0.6449046096788074 1105 78-79 0.59255648755056 1105 80-81 0.5930383026431265 1105 82-83 0.5516075514246346 1105 84-85 0.3090233618520486 1105 86-87 0.25003513956133716 1105 88-89 0.4321615388417328 1105 90-91 0.5205177500543599 1105 92-93 0.5551276796322675 1105 94-95 0.878647985759482 1105 96-97 0.4178745210017816 1105 98-99 0.6072058929897501 1105 100-101 0.6075981618861128 1106 1 0.40420832830591635 1106 2 0.4089379665923367 1106 3 0.34617990404758814 1106 4 0.2661514766847404 1106 5 0.3817773123248216 1106 6 0.35032080814874433 1106 7 0.27855162628587493 1106 8 0.3416901791750959 1106 9 0.4249587947567832 1106 10-11 0.43502674924745577 1106 12-13 0.4204399644248866 1106 14-15 0.692673174635182 1106 16-17 0.613920084229612 1106 18-19 0.5376453063013358 1106 20-21 0.5507063090242497 1106 22-23 0.519981244861242 1106 24-25 0.6358600926311979 1106 26-27 0.5813582458965598 1106 28-29 0.677583334635024 1106 30-31 0.6655246456443606 1106 32-33 0.7379610376695851 1106 34-35 0.8073686485471256 1106 36-37 0.9396796756290016 1106 38-39 0.908448224441571 1106 40-41 0.6657548534391609 1106 42-43 0.6203355665826109 1106 44-45 0.7580309081879903 1106 46-47 0.8364562769877963 1106 48-49 0.8336312018514249 1106 50-51 0.8905388183257088 1106 52-53 0.880251388374937 1106 54-55 0.9513129036687502 1106 56-57 0.8756196061497334 1106 58-59 0.8921126916939741 1106 60-61 0.9871387475903077 1106 62-63 0.9506591313498376 1106 64-65 0.984830237219235 1106 66-67 1.4604967994839804 1106 68-69 2.958902179340445 1106 70-71 3.3073958566382586 1106 72-73 2.124294965534286 1106 74-75 1.635912013206827 1106 76-77 1.1289693416638151 1106 78-79 1.158567849311865 1106 80-81 1.1132088174582044 1106 82-83 1.1167007054920006 1106 84-85 0.966333738888089 1106 86-87 0.9863074741439952 1106 88-89 1.0877590331254936 1106 90-91 1.0455072162637258 1106 92-93 0.9349775694326112 1106 94-95 1.3321073415334688 1106 96-97 1.2442682225316126 1106 98-99 1.2526343015181425 1106 100-101 1.4726347428681272 1107 1 0.05781035998038675 1107 2 0.09814969327480227 1107 3 0.12675255880742853 1107 4 0.2009800270575539 1107 5 0.07994685454781347 1107 6 0.09883907780560719 1107 7 0.06908516962634081 1107 8 0.13016399090081876 1107 9 0.09779682916713028 1107 10-11 0.181213446066522 1107 12-13 0.12214223837822402 1107 14-15 0.18641570878542524 1107 16-17 0.13190435296167635 1107 18-19 0.20709270096060095 1107 20-21 0.23863740163932334 1107 22-23 0.23240965699541505 1107 24-25 0.2642459157050112 1107 26-27 0.30105080942853846 1107 28-29 0.43732386023084757 1107 30-31 0.11761887671000437 1107 32-33 0.14422338040506588 1107 34-35 0.22471807921587583 1107 36-37 0.13829626571049403 1107 38-39 0.34016068566732116 1107 40-41 0.20551110548625218 1107 42-43 0.29575895251406337 1107 44-45 0.2696475295778953 1107 46-47 0.23917414734210496 1107 48-49 0.30443160115396495 1107 50-51 0.23785707305086845 1107 52-53 0.24265156273865784 1107 54-55 0.05813687097314357 1107 56-57 0.18588015226460897 1107 58-59 0.09174861110133037 1107 60-61 0.13056103291100385 1107 62-63 0.14400176087028882 1107 64-65 -0.018650113145866953 1107 66-67 0.6298719439843019 1107 68-69 2.1462922988903763 1107 70-71 2.3582635810472503 1107 72-73 1.2595288995072735 1107 74-75 0.6079244089257649 1107 76-77 0.24265105322191616 1107 78-79 0.21832836411833512 1107 80-81 0.3211742770655448 1107 82-83 0.46112112675859507 1107 84-85 0.38441259511990467 1107 86-87 0.3837092380112068 1107 88-89 0.3670311850242278 1107 90-91 0.3115067129209521 1107 92-93 0.4836154733110014 1107 94-95 0.7912348245306013 1107 96-97 0.5289747905527271 1107 98-99 0.7349983387606898 1107 100-101 0.6925536902314775 1108 1 -0.216084789697657 1108 2 -0.2458407706103607 1108 3 -0.20050951066796685 1108 4 -0.12810394966428618 1108 5 0.0024022574269579877 1108 6 0.06894454305793118 1108 7 0.01253181913597956 1108 8 0.01607416412576157 1108 9 -0.12623468484072475 1108 10-11 -0.13643974963507333 1108 12-13 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-0.6744992584563576 2316 60-61 -0.6853104205291487 2316 62-63 -0.5913205169485067 2316 64-65 -0.2873918534096376 2316 66-67 0.10596701411800424 2316 68-69 1.3681652197153902 2316 70-71 1.6220838856435371 2316 72-73 0.6527237882685633 2316 74-75 0.16663529765059693 2316 76-77 -0.21377467978695108 2316 78-79 -0.222083506204946 2316 80-81 -0.44041099986916876 2316 82-83 -0.45612224038839244 2316 84-85 -0.5811147975832682 2316 86-87 -0.4891657881920928 2316 88-89 -0.5352143239578062 2316 90-91 -0.4963613409256915 2316 92-93 -0.49056083161339714 2316 94-95 -0.02944755500257301 2316 96-97 -0.30940165290738264 2316 98-99 -0.3466272229013718 2316 100-101 -0.36645819467450735 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 8.0 4 36.0 5 89.0 6 180.0 7 562.0 8 1154.0 9 2080.0 10 3378.0 11 5038.0 12 7445.0 13 9444.0 14 11469.0 15 14106.0 16 17345.0 17 20453.0 18 19505.0 19 17758.0 20 18392.0 21 19425.0 22 21017.0 23 24344.0 24 29704.0 25 36585.0 26 45441.0 27 56843.0 28 69814.0 29 84642.0 30 100883.0 31 120261.0 32 144206.0 33 174380.0 34 214716.0 35 270377.0 36 342978.0 37 386776.0 38 233466.0 39 19049.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.610308035298196 20.060185787832513 14.807221822617885 23.522284354251408 2 15.418512944163535 21.170403924287644 39.6682462031935 23.74283692835532 3 19.153085497473803 24.938368686967976 30.23579137751391 25.672754438044308 4 12.98809091777665 17.32496432858514 37.57181956260102 32.11512519103719 5 13.35264904242549 38.07080652980534 34.779560682296164 13.796983745473005 6 31.67803748753613 37.08833853906184 17.53198141665015 13.701642556751878 7 28.287618745805243 32.598817387912725 21.1858125867703 17.92775127951173 8 25.78242186593839 36.04639230181576 19.82331984588848 18.347865986357377 9 26.069090684619873 16.757367144867423 20.197235696342585 36.97630647417012 10-11 24.662148746163037 26.90098464507286 27.96043254711399 20.47643406165011 12-13 25.646420296356816 24.080338041946987 28.14670240426712 22.12653925742907 14-15 22.35322819915285 25.624697004308878 26.25217576954516 25.76989902699311 16-17 22.128214297439573 28.96311489187363 26.750535269813142 22.15813554087366 18-19 22.00678042072422 27.912705769255343 28.560669431390263 21.519844378630175 20-21 22.812090968103917 26.971088952261052 28.48637212372801 21.730447955907024 22-23 22.473876021142168 27.029122579523957 28.23972920786065 22.257272191473223 24-25 22.4053095274839 27.1020827824692 28.360559537067708 22.132048152979188 26-27 22.271686856297627 27.20076938093498 28.444879741961714 22.082664020805684 28-29 22.07383962097234 27.54294532801227 28.432244873886802 21.95097017712859 30-31 22.305048599498534 27.170050866203837 28.332007648213626 22.192892886084 32-33 22.070701927458728 27.179307017623188 28.34556876369531 22.40442229122277 34-35 22.59347801725748 27.19340842113127 28.17752516961598 22.03558839199527 36-37 22.51842440694457 26.889902643719623 28.362025009057472 22.229647940278337 38-39 22.225672413399543 27.233441246917454 28.416909295129877 22.12397704455312 40-41 22.454853781789115 27.022807592743543 28.393164216478283 22.12917440898906 42-43 22.163512838547643 26.998794412022974 28.2292970867122 22.608395662717182 44-45 22.20859576615165 27.038632183686378 28.172326215784025 22.580445834377947 46-47 22.389773697282582 27.172560003494716 27.8812441041379 22.556422195084807 48-49 22.232251277622293 27.248543409247837 27.937084960923087 22.582120352206783 50-51 22.12023623946814 27.261282158347505 28.074609766857762 22.54387183532659 52-53 22.36845574021152 27.332093112564593 27.724816573630434 22.57463457359345 54-55 21.963824035254166 27.06656639535316 28.05725379218934 22.91235577720333 56-57 22.18127161755347 27.274774296419185 27.883526962108856 22.660427123918485 58-59 22.328716568466284 27.038559945293272 27.763958876365034 22.868764609875406 60-61 22.20978561766649 27.11871637987983 28.014191229670644 22.657306772783038 62-63 22.10451651131413 27.070188099995224 27.994341213576135 22.830954175114513 64-65 21.819088682323006 27.088098026544593 27.903852169062997 23.1889611220694 66-67 21.592054475540216 27.177990829512293 27.948072364429912 23.28188233051758 68-69 21.51158452006932 26.974525071989895 28.26536820962416 23.248522198316625 70-71 21.559568197923102 26.979458016939684 28.04137609771407 23.419597687423142 72-73 21.682969895897653 26.83313588256554 27.95562133965176 23.528272881885048 74-75 21.725471132844586 27.145507107526623 27.496699764396702 23.632321995232086 76-77 21.693686996810264 26.926801257015647 27.648444659453382 23.73106708672071 78-79 21.77004223598479 26.978281978625052 27.56364445648522 23.68803132890494 80-81 21.763157646407517 27.083267801984185 27.41136360776814 23.74221094384016 82-83 21.74216477225143 27.03034144296918 27.393621726909316 23.833872057870064 84-85 21.68929605292259 26.86481837349546 27.376449252212893 24.069436321369068 86-87 21.636571202994823 26.910851096999654 27.492924120742014 23.95965357926351 88-89 21.645067516307734 26.748084267649517 27.378178806404236 24.228669409638513 90-91 21.575767226660297 26.79812804656882 27.357610536569638 24.268494190201242 92-93 21.672781118483666 26.698945048178707 27.408262088714565 24.22001174462306 94-95 21.294094917004283 26.55107392332222 27.569475268126688 24.585355891546808 96-97 21.375194781574276 26.386849724479312 27.615224668564096 24.622730825382313 98-99 21.2731526996736 26.47711274529081 27.36617641275709 24.8835581422785 100-101 21.150701500756856 26.411500054952974 27.543197067654283 24.894601376635883 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 224.0 1 152.5 2 96.5 3 119.0 4 124.0 5 140.5 6 224.0 7 467.0 8 731.5 9 861.5 10 916.5 11 967.5 12 1066.5 13 1207.5 14 1339.0 15 1467.0 16 1603.5 17 1762.5 18 1978.0 19 2168.0 20 2471.5 21 2996.0 22 3657.5 23 4472.0 24 5437.5 25 6920.0 26 9313.5 27 12771.0 28 16254.0 29 20319.5 30 25512.5 31 30683.0 32 36217.0 33 42219.0 34 48308.5 35 54305.5 36 61454.5 37 68572.0 38 74136.5 39 78364.5 40 81934.0 41 86376.0 42 90866.0 43 95804.5 44 100941.5 45 104608.0 46 106055.5 47 109484.0 48 116766.5 49 115686.0 50 107466.5 51 101225.0 52 94534.0 53 88828.5 54 84167.0 55 76365.0 56 67318.5 57 59215.5 58 51672.0 59 44330.0 60 36038.0 61 27707.0 62 21012.0 63 15614.5 64 11153.5 65 7948.0 66 5512.5 67 3912.0 68 2722.5 69 1787.5 70 1239.0 71 926.0 72 683.0 73 541.5 74 412.0 75 259.0 76 158.5 77 83.5 78 45.5 79 25.0 80 16.5 81 10.5 82 6.0 83 3.5 84 1.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02201817836533112 2 0.024534541607083248 3 1.1795452695713101E-4 4 0.0 5 0.0 6 3.9318175652377E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.735226686975815E-4 18-19 1.96590878261885E-5 20-21 0.0 22-23 0.0 24-25 1.96590878261885E-5 26-27 3.735226686975815E-4 28-29 1.1795452695713101E-4 30-31 5.50454459133278E-4 32-33 0.002201817836533112 34-35 0.004246362970456716 36-37 0.10354441558053484 38-39 0.09558248501092849 40-41 0.10474361993793233 42-43 0.10552998345097987 44-45 0.10002543885964708 46-47 0.09341998535004775 48-49 0.09446191700483574 50-51 0.09326271264743824 52-53 0.07352498846994499 54-55 0.0622210129698866 56-57 0.033774312885391844 58-59 0.013309202458329613 60-61 0.009161134927003841 62-63 0.0020248860460974154 64-65 0.004855794693068559 66-67 0.15772486162951033 68-69 0.4209403885343481 70-71 0.4029326640855595 72-73 0.18658440255835504 74-75 0.028761245489713778 76-77 0.03235885856190627 78-79 0.005602840030463722 80-81 0.001612045201747457 82-83 0.0 84-85 6.094317226118435E-4 86-87 0.0011598861817451215 88-89 1.769317904356965E-4 90-91 8.060226008737285E-4 92-93 0.0024180678026211855 94-95 0.0793047602908444 96-97 0.01731965637487207 98-99 0.017811133570526782 100-101 0.0105962483383156 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2543353.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.256197108454614 #Duplication Level Percentage of deduplicated Percentage of total 1 71.55986303842182 36.67886444951365 2 14.694207915602057 15.06338434549426 3 5.643939926217582 8.678606919794557 4 2.749963807956307 5.638107479268996 5 1.4982318672163835 3.839683395010548 6 0.9153167223450619 2.8149392602309873 7 0.6207932391636427 2.2273650441117376 8 0.41749555254296333 1.711938746643624 9 0.30837143897764857 1.4225352532970525 >10 1.456722420730914 13.19540712586611 >50 0.08945981685187655 3.1739282208060193 >100 0.04279278934121452 3.9425423970524385 >500 0.0019199085356205446 0.6026457560721565 >1k 9.215560970892389E-4 1.0100516068379009 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4926 0.19368133326360912 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4155 0.16336701983562643 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3620 0.14233179586160474 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2846 0.11189952790666494 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 3.9318175652377E-5 8 0.0 0.0 0.0 0.0 3.9318175652377E-5 9 0.0 0.0 0.0 0.0 3.9318175652377E-5 10-11 0.0 0.0 0.0 3.9318175652377E-5 3.9318175652377E-5 12-13 0.0 0.0 0.0 7.8636351304754E-5 3.9318175652377E-5 14-15 0.0 0.0 0.0 1.17954526957131E-4 3.9318175652377E-5 16-17 0.0 0.0 0.0 1.17954526957131E-4 3.9318175652377E-5 18-19 0.0 0.0 0.0 1.17954526957131E-4 3.9318175652377E-5 20-21 0.0 0.0 0.0 1.96590878261885E-4 3.9318175652377E-5 22-23 0.0 0.0 0.0 2.75227229566639E-4 3.9318175652377E-5 24-25 0.0 0.0 0.0 7.47045337395163E-4 3.9318175652377E-5 26-27 0.0 0.0 0.0 0.0021035223974021694 3.9318175652377E-5 28-29 0.0 0.0 0.0 0.006625112597425525 3.9318175652377E-5 30-31 0.0 0.0 0.0 0.024731132485345135 3.9318175652377E-5 32-33 0.0 0.0 0.0 0.06861021651339787 3.9318175652377E-5 34-35 0.0 0.0 0.0 0.1304380477267607 3.9318175652377E-5 36-37 0.0 0.0 0.0 0.23443462232729784 3.9318175652377E-5 38-39 0.0 0.0 0.0 0.4009667553029406 3.9318175652377E-5 40-41 0.0 0.0 0.0 0.5843860447212794 3.9318175652377E-5 42-43 0.0 0.0 0.0 0.7637555620474232 3.9318175652377E-5 44-45 0.0 0.0 0.0 0.9840356411398654 3.9318175652377E-5 46-47 0.0 0.0 0.0 1.242768896020332 3.9318175652377E-5 48-49 0.0 0.0 0.0 1.5143591943391264 3.9318175652377E-5 50-51 0.0 0.0 0.0 1.8044486943023639 3.9318175652377E-5 52-53 0.0 0.0 0.0 2.0808751282264004 3.9318175652377E-5 54-55 0.0 0.0 0.0 2.379182126900985 3.9318175652377E-5 56-57 0.0 0.0 0.0 2.726007754330602 3.9318175652377E-5 58-59 0.0 0.0 0.0 3.080382471485476 3.9318175652377E-5 60-61 0.0 0.0 0.0 3.440772869515164 3.9318175652377E-5 62-63 0.0 0.0 0.0 3.7924542916378496 3.9318175652377E-5 64-65 0.0 0.0 0.0 4.157739802536259 3.9318175652377E-5 66-67 0.0 0.0 0.0 4.534211334407768 3.9318175652377E-5 68-69 3.9318175652377E-5 0.0 0.0 4.962091380944761 3.9318175652377E-5 70-71 3.9318175652377E-5 0.0 0.0 5.449636759034236 3.9318175652377E-5 72-73 3.9318175652377E-5 0.0 0.0 5.952123043871614 3.9318175652377E-5 74-75 3.9318175652377E-5 0.0 0.0 6.45549398766117 3.9318175652377E-5 76-77 3.9318175652377E-5 0.0 0.0 6.984284132009988 3.9318175652377E-5 78-79 3.9318175652377E-5 0.0 0.0 7.572307107979112 3.9318175652377E-5 80-81 3.9318175652377E-5 0.0 0.0 8.201948372876277 3.9318175652377E-5 82-83 3.9318175652377E-5 0.0 0.0 8.826478274938633 3.9318175652377E-5 84-85 3.9318175652377E-5 0.0 0.0 9.444933518862697 3.9318175652377E-5 86-87 3.9318175652377E-5 0.0 0.0 10.054443877825847 3.9318175652377E-5 88-89 3.9318175652377E-5 0.0 0.0 10.710565934024888 3.9318175652377E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10085 0.0 24.891062 1 TACATGG 10155 0.0 24.247904 2 ACATGGG 10355 0.0 22.995691 3 GTATCAA 11780 0.0 22.799997 1 GGTATCA 8895 0.0 22.35279 1 ATCAACG 13375 0.0 19.753963 3 TCAACGC 13480 0.0 19.635283 4 CAACGCA 13635 0.0 19.342497 5 TATCAAC 13875 0.0 19.114616 2 AACGCAG 13860 0.0 19.028494 6 GAGTACT 5550 0.0 18.589132 12-13 CATGGGA 5975 0.0 18.497408 4 ATCGCCG 120 2.8744907E-6 17.95056 64-65 CCGCGCA 160 0.0012026597 17.787878 9 AGTACTT 5975 0.0 17.743221 12-13 CATGGGG 4780 0.0 17.5646 4 TACACTG 2215 0.0 17.132042 5 ACGCAGA 15360 0.0 17.077599 7 CGCAGAG 15515 0.0 16.906986 8 GTATAGG 815 0.0 16.885115 1 >>END_MODULE