FastQCFastQC Report
Fri 27 May 2016
SRR939789_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939789_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2756788
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45580.16533734186306673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34410.1248191736179931No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41150.043.811911
GTATCAA69500.037.886691
TCAACGC87450.030.2744664
ATCAACG87800.030.153783
TATCAAC90350.029.7212262
CAACGCA89150.029.5374985
AACGCAG93100.028.488156
GTACATG88800.025.110961
ACGCAGA105750.024.8560147
CGCAGAG108050.024.2816838
TACATGG91950.023.9927272
ACATGGG94450.022.0026133
GCAGAGT115800.020.7310079
AGAGTAC100000.019.61689610-11
GAGTACT59350.019.46421812-13
TGGTATC25450.019.3875982
GTACTTT62300.018.6579714-15
CATGGGG60300.018.6480374
CAGAGTA110500.018.2895110-11
AGTACTT63800.017.92070612-13
Produced by FastQC (version 0.11.5)