FastQCFastQC Report
Fri 27 May 2016
SRR939789_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939789_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2756788
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49930.18111657479646603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42590.1544913863525233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28780.10439685605131768No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45100.046.8251951
GTATCAA74550.036.3578571
TCAACGC90100.029.6024634
ATCAACG90800.029.3753173
CAACGCA93300.028.6376675
TATCAAC93850.028.5221212
AACGCAG96800.027.6358496
ACGCAGA111550.023.770527
CGCAGAG112900.023.1525528
GTACATG94750.022.8355541
TACATGG94400.021.965872
ACATGGG96400.020.7760933
GCAGAGT126950.020.2562839
GTACTTT61750.019.52659414-15
GAGTACT63500.019.24754312-13
GGACCGA6450.018.9851266
AGAGTAC109650.018.38378510-11
CAGAGTA120250.018.32992210-11
CATGGGG62250.016.9568374
AGTACTT67600.016.8957212-13
Produced by FastQC (version 0.11.5)