##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939789_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2756788 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.385046655745743 31.0 31.0 34.0 30.0 34.0 2 31.595390360085723 31.0 31.0 34.0 30.0 34.0 3 31.6616547953633 31.0 31.0 34.0 30.0 34.0 4 35.479371645552725 37.0 35.0 37.0 33.0 37.0 5 35.19860032762766 37.0 35.0 37.0 32.0 37.0 6 35.21567744781245 37.0 35.0 37.0 32.0 37.0 7 35.13053524609074 37.0 35.0 37.0 32.0 37.0 8 35.06598149730774 37.0 35.0 37.0 32.0 37.0 9 36.6768463878978 39.0 35.0 39.0 32.0 39.0 10-11 36.52378528925692 38.0 35.0 39.0 32.0 39.0 12-13 36.47066277856694 38.0 35.0 39.0 32.0 39.0 14-15 37.51868533235054 39.0 36.0 41.0 32.0 41.0 16-17 37.44010584056518 39.0 36.0 41.0 32.0 41.0 18-19 37.38767435145539 39.0 36.0 41.0 32.0 41.0 20-21 37.29023795083263 39.0 36.0 40.0 31.0 41.0 22-23 37.148650530980255 39.0 36.0 40.0 31.0 41.0 24-25 36.9951376384401 39.0 36.0 40.0 31.0 41.0 26-27 36.69690451351355 38.5 35.5 40.0 30.0 41.0 28-29 36.47408796033645 38.0 35.0 40.0 30.0 41.0 30-31 36.23855425226749 38.0 35.0 40.0 30.0 41.0 32-33 36.238670329383325 38.0 35.0 40.0 30.0 41.0 34-35 36.36098151181737 38.0 35.0 40.0 30.0 41.0 36-37 36.35309624824252 38.0 35.0 40.0 30.0 41.0 38-39 36.24143187651716 38.0 35.0 40.0 30.0 41.0 40-41 36.0832013560709 38.0 35.0 40.0 29.5 41.0 42-43 35.900894628096175 38.0 35.0 40.0 29.0 41.0 44-45 35.686083043019636 38.0 34.0 40.0 28.0 41.0 46-47 35.43050716993835 38.0 34.0 40.0 27.0 41.0 48-49 35.15874289209036 38.0 34.0 40.0 27.0 41.0 50-51 34.906362767104326 38.0 33.0 40.0 26.0 41.0 52-53 34.61967042804888 38.0 33.0 40.0 26.0 41.0 54-55 34.30989162024791 37.0 33.0 40.0 25.0 41.0 56-57 33.040418414473656 36.5 31.5 39.5 18.5 41.0 58-59 29.725592247209434 34.5 25.5 38.5 8.0 40.0 60-61 29.747673016568555 34.0 25.5 38.0 10.0 40.0 62-63 31.46849775898618 35.0 29.5 38.0 14.5 40.0 64-65 30.929117146476262 35.0 29.0 38.0 7.5 40.0 66-67 25.37872553130672 28.5 18.0 36.0 2.0 39.0 68-69 17.560892059889987 13.0 7.0 29.0 2.0 35.5 70-71 16.420537596652338 15.0 7.5 25.5 2.0 33.0 72-73 18.56339787462801 19.0 7.5 30.0 2.0 34.0 74-75 19.328949487592084 19.0 8.5 30.5 2.0 35.0 76-77 20.426266909171105 23.5 9.5 31.0 2.0 34.5 78-79 23.65066029741859 29.0 13.0 34.0 2.0 35.5 80-81 26.312937919056523 31.5 23.0 34.5 2.0 36.0 82-83 27.202491812935925 33.0 26.0 35.0 2.0 36.0 84-85 27.16765126661898 33.0 26.0 35.0 2.0 36.0 86-87 26.866546865410037 33.0 25.5 35.0 2.0 35.0 88-89 26.419137960554096 33.0 25.0 35.0 2.0 35.0 90-91 24.877894673076057 31.5 15.0 34.5 2.0 35.0 92-93 22.821292569468525 31.0 2.0 34.0 2.0 35.0 94-95 18.2415561153052 23.5 2.0 33.0 2.0 35.0 96-97 16.8663890730807 15.0 2.0 32.5 2.0 34.5 98-99 15.714747017180864 4.5 2.0 32.0 2.0 34.0 100-101 15.078729303812988 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.49825312759978146 1101 2 0.46168245836649646 1101 3 0.43015590292115036 1101 4 0.38004360504415047 1101 5 0.41215042709897176 1101 6 0.4348291843322869 1101 7 0.4098931050919745 1101 8 0.507379368899791 1101 9 0.548448101174003 1101 10-11 0.5497870291936735 1101 12-13 0.5297377285827096 1101 14-15 0.5633566458649781 1101 16-17 0.5500642371083302 1101 18-19 0.6977603220458164 1101 20-21 0.8005313853022642 1101 22-23 0.717520649600857 1101 24-25 0.6018929790169523 1101 26-27 0.6381830906899282 1101 28-29 0.6145211786173164 1101 30-31 0.8606983288159213 1101 32-33 0.43522430210411756 1101 34-35 0.8195043676845088 1101 36-37 0.5530979496839237 1101 38-39 0.40020407124717394 1101 40-41 0.3000871002106251 1101 42-43 0.23110213257125878 1101 44-45 0.5095565684649657 1101 46-47 0.3385461502517586 1101 48-49 0.6202473625116625 1101 50-51 0.5274975464161002 1101 52-53 0.6967261415656409 1101 54-55 0.5592636340043526 1101 56-57 1.2227450744749788 1101 58-59 -8.340388260801735 1101 60-61 -9.47170842806226 1101 62-63 -2.590605464034482 1101 64-65 -0.07702827090700382 1101 66-67 -0.7116381993600172 1101 68-69 -3.3791872157736 1101 70-71 -3.8542209313147673 1101 72-73 -6.5430457335108265 1101 74-75 -7.910551358246941 1101 76-77 -8.879865388024896 1101 78-79 -11.997389762686037 1101 80-81 -8.176434757117896 1101 82-83 -4.062571198444349 1101 84-85 -2.9609695648210383 1101 86-87 -2.7422463393120395 1101 88-89 -2.8274187425603614 1101 90-91 -8.582823231008774 1101 92-93 -14.771061860944943 1101 94-95 -12.199324607016813 1101 96-97 -12.257057842759096 1101 98-99 -12.032586034623549 1101 100-101 -11.859612910980141 1102 1 0.5021871476320747 1102 2 0.5212038903470742 1102 3 0.4929553464309677 1102 4 0.45760831733804963 1102 5 0.501758886832981 1102 6 0.5126159511040029 1102 7 0.5168888366362339 1102 8 0.6140602976020233 1102 9 0.8268590576668942 1102 10-11 0.6985005687623627 1102 12-13 0.7446021771869198 1102 14-15 0.8944683060945522 1102 16-17 0.8640221889265689 1102 18-19 0.9231100747732626 1102 20-21 0.8856364719068921 1102 22-23 0.9045565269699125 1102 24-25 0.9284399980371347 1102 26-27 1.1915073071524276 1102 28-29 0.9920682890318773 1102 30-31 0.9235035267862912 1102 32-33 0.7688736794376325 1102 34-35 0.9375913141984924 1102 36-37 0.8372020986499891 1102 38-39 0.7624954297399142 1102 40-41 0.7133981829631466 1102 42-43 0.8169749319132151 1102 44-45 0.8107118610454336 1102 46-47 0.9384269990685397 1102 48-49 0.8629647541839418 1102 50-51 0.8641984803311971 1102 52-53 0.8838788006341645 1102 54-55 0.7548679031370824 1102 56-57 1.395993941354 1102 58-59 -9.242366779738983 1102 60-61 -10.271449138839689 1102 62-63 -2.9743156017014876 1102 64-65 -0.09180606410523495 1102 66-67 -1.2894594928950482 1102 68-69 -3.81297910063023 1102 70-71 -4.60000511196421 1102 72-73 -7.144803890458661 1102 74-75 -8.018818487075773 1102 76-77 -8.860850280873457 1102 78-79 -11.798779295660786 1102 80-81 -7.878458634744213 1102 82-83 -3.7976933791818013 1102 84-85 -2.9711326191532983 1102 86-87 -2.7350265223038868 1102 88-89 -2.8647242333312235 1102 90-91 -8.661582501613974 1102 92-93 -14.333820684733205 1102 94-95 -12.427470689075419 1102 96-97 -12.213827965599904 1102 98-99 -11.692170765670218 1102 100-101 -11.440954580382996 1103 1 0.2763989131799569 1103 2 0.23777814039511824 1103 3 0.2767847026905521 1103 4 0.1506108780155202 1103 5 0.15489994290415865 1103 6 0.2537847450210009 1103 7 0.2828909274492375 1103 8 0.2880692255860211 1103 9 0.333495217623593 1103 10-11 0.372645367906955 1103 12-13 0.4464893884676897 1103 14-15 0.42172398635709385 1103 16-17 0.4310693880329879 1103 18-19 0.481395820583856 1103 20-21 0.4209088302146142 1103 22-23 0.3579606400452917 1103 24-25 0.466997586814216 1103 26-27 0.323235142866487 1103 28-29 0.4005518867361957 1103 30-31 0.3265889017748478 1103 32-33 0.38127463467339595 1103 34-35 0.4294431449272551 1103 36-37 0.2013322427200066 1103 38-39 0.2449446593094251 1103 40-41 0.3013352468451487 1103 42-43 0.5241611184570516 1103 44-45 0.4083255981602747 1103 46-47 0.31492020728111925 1103 48-49 0.2680518524437687 1103 50-51 0.34060703466018083 1103 52-53 0.439903288754941 1103 54-55 0.32367546316501716 1103 56-57 0.9555877965481372 1103 58-59 -6.721924693376739 1103 60-61 -8.528457639826666 1103 62-63 -2.287004160212849 1103 64-65 0.024766359416915407 1103 66-67 -1.7046213724796502 1103 68-69 -4.244946695824378 1103 70-71 -4.800882701740287 1103 72-73 -7.462340477356468 1103 74-75 -8.153808069354895 1103 76-77 -9.092393842490841 1103 78-79 -12.041189008901814 1103 80-81 -8.396083491301358 1103 82-83 -4.634381090036044 1103 84-85 -3.738050023093418 1103 86-87 -3.4562973668316808 1103 88-89 -3.478357813972437 1103 90-91 -8.974756673717817 1103 92-93 -14.462272515797453 1103 94-95 -12.544961128237476 1103 96-97 -12.345685335548 1103 98-99 -12.083403292294163 1103 100-101 -11.743588579820502 1104 1 0.16698201129580426 1104 2 0.19522026081038746 1104 3 0.161277075210716 1104 4 0.14112047569309993 1104 5 0.08442733669441793 1104 6 0.17327619275446438 1104 7 0.22295381294456007 1104 8 0.22792656718614523 1104 9 0.20575981920136144 1104 10-11 0.22547175729843616 1104 12-13 0.14332324048573497 1104 14-15 0.1772264671979329 1104 16-17 0.2523348178979745 1104 18-19 0.208081143816905 1104 20-21 0.20827881726759756 1104 22-23 0.29977370446673035 1104 24-25 0.3235852043224412 1104 26-27 0.1811079726333631 1104 28-29 0.11292826687981972 1104 30-31 0.16195435335221475 1104 32-33 0.22864526505625804 1104 34-35 0.1421030727134749 1104 36-37 0.19956501555218864 1104 38-39 0.3008963452357847 1104 40-41 0.32513748724143454 1104 42-43 0.2321536879969912 1104 44-45 0.12036519511410404 1104 46-47 0.021024121139248564 1104 48-49 0.116716862536002 1104 50-51 0.13371632836440028 1104 52-53 0.12918123138600635 1104 54-55 -0.03337937266523738 1104 56-57 0.48024373568913603 1104 58-59 -6.769493951858646 1104 60-61 -8.856220379768605 1104 62-63 -2.7969352434999415 1104 64-65 -0.45526886289376023 1104 66-67 -1.8899187115064535 1104 68-69 -4.3076502896258955 1104 70-71 -4.975098201165309 1104 72-73 -7.947489528846983 1104 74-75 -8.973588211369965 1104 76-77 -9.32439194466771 1104 78-79 -12.367604455252124 1104 80-81 -8.669731091278841 1104 82-83 -4.92410276944868 1104 84-85 -4.022510872449683 1104 86-87 -3.847817635743567 1104 88-89 -3.877216707605882 1104 90-91 -9.34007652929026 1104 92-93 -14.93799823210506 1104 94-95 -12.685682622692777 1104 96-97 -12.348760291520112 1104 98-99 -11.663948675196064 1104 100-101 -11.253475916676067 1105 1 0.15872248015680412 1105 2 0.29599718536188036 1105 3 0.17526983594276402 1105 4 0.1594301855585698 1105 5 0.1596534792471047 1105 6 0.07115154654267997 1105 7 0.08603873339154688 1105 8 0.16285344155890868 1105 9 0.19942354280114927 1105 10-11 0.21440176643208986 1105 12-13 0.2414967348420376 1105 14-15 0.3069582083433531 1105 16-17 0.342718318681662 1105 18-19 0.2807275360228587 1105 20-21 0.22841210098600584 1105 22-23 0.328543818242764 1105 24-25 0.3349386768030769 1105 26-27 0.2521006093281031 1105 28-29 0.11597236777852515 1105 30-31 0.2027152658157121 1105 32-33 0.20246347147687516 1105 34-35 0.25774079358444624 1105 36-37 0.16071157126184943 1105 38-39 0.2165807492365559 1105 40-41 0.06434139328882083 1105 42-43 0.28790281691685493 1105 44-45 0.29441929792974975 1105 46-47 0.31336267531536066 1105 48-49 0.4893706969800391 1105 50-51 0.47018194184161644 1105 52-53 0.5323804916726402 1105 54-55 0.3925166840761847 1105 56-57 0.9140141313681269 1105 58-59 -6.7223577476255905 1105 60-61 -8.799991113731142 1105 62-63 -2.6789187127105016 1105 64-65 -0.2026159305391957 1105 66-67 -1.7569329081394045 1105 68-69 -4.389028005760892 1105 70-71 -5.010754189347939 1105 72-73 -6.313319056490386 1105 74-75 -6.97370300225435 1105 76-77 -8.678639545673539 1105 78-79 -12.066947734121804 1105 80-81 -8.408921240461886 1105 82-83 -4.370187588410602 1105 84-85 -3.382262346802154 1105 86-87 -3.3086453028274505 1105 88-89 -3.5645998588299044 1105 90-91 -8.831305738947126 1105 92-93 -11.687013037648402 1105 94-95 -11.993993798453634 1105 96-97 -12.19945681734806 1105 98-99 -11.689098252905433 1105 100-101 -11.104098696690274 1106 1 0.1788424915806459 1106 2 0.19412601862681456 1106 3 0.22181675300013382 1106 4 0.16455782342193004 1106 5 0.18686451944283533 1106 6 0.1634154285498468 1106 7 0.09100186444914726 1106 8 0.23561803079261523 1106 9 0.11588048510834881 1106 10-11 0.32432733037516925 1106 12-13 0.3592921167389207 1106 14-15 0.43920193499413784 1106 16-17 0.3892195272830534 1106 18-19 0.33047742676982494 1106 20-21 0.19059222633845252 1106 22-23 0.24157928080470725 1106 24-25 0.3546093490140976 1106 26-27 0.4418279011162696 1106 28-29 0.5698316996234425 1106 30-31 0.48374558023097336 1106 32-33 0.4982488900212445 1106 34-35 0.45743972867560245 1106 36-37 0.5416980971944767 1106 38-39 0.38210492268257923 1106 40-41 0.39904096218935337 1106 42-43 0.4742777916353873 1106 44-45 0.6076346925377294 1106 46-47 0.4687174263875491 1106 48-49 0.39992623035408315 1106 50-51 0.3474462285704334 1106 52-53 0.3890019015134172 1106 54-55 0.34211934777736985 1106 56-57 1.2229561671551252 1106 58-59 -6.403908789352936 1106 60-61 -8.686758177548658 1106 62-63 -2.165329446932301 1106 64-65 0.21568069530403733 1106 66-67 -1.316933601947948 1106 68-69 -4.214018073171593 1106 70-71 -4.7070898984300875 1106 72-73 -6.154517080576017 1106 74-75 -6.703864496011054 1106 76-77 -8.871779153697894 1106 78-79 -11.969334217335021 1106 80-81 -8.021004701702534 1106 82-83 -4.043780558864611 1106 84-85 -3.005927501708001 1106 86-87 -2.7541322418816065 1106 88-89 -2.8742558846989255 1106 90-91 -8.639448913103763 1106 92-93 -12.169087141200853 1106 94-95 -11.834554195032851 1106 96-97 -12.146396390769258 1106 98-99 -11.827646691978025 1106 100-101 -11.562844578838384 1107 1 0.08360087415266548 1107 2 0.0966490338968029 1107 3 0.03935683470376006 1107 4 0.028971745946769545 1107 5 0.013767277864829452 1107 6 0.09124699160839356 1107 7 0.08352753496521359 1107 8 0.12209175548279205 1107 9 -0.015465835382727278 1107 10-11 0.19319781438069583 1107 12-13 0.2629492033338252 1107 14-15 0.18562360888872576 1107 16-17 0.18889759807293416 1107 18-19 0.26945925173219365 1107 20-21 0.14404613899525032 1107 22-23 0.1255992835108799 1107 24-25 0.17692311583388687 1107 26-27 0.39342380588085035 1107 28-29 0.5119377017418785 1107 30-31 0.45413482513346537 1107 32-33 0.2536624141484438 1107 34-35 0.2412439012874117 1107 36-37 0.1887813459135188 1107 38-39 0.2833639832392407 1107 40-41 0.08193138910818476 1107 42-43 0.13720227391223005 1107 44-45 0.1255539495825957 1107 46-47 0.10008567092884846 1107 48-49 0.1625602964748012 1107 50-51 0.13051572404488354 1107 52-53 -0.01866485026580733 1107 54-55 -0.014583509812197804 1107 56-57 0.8137703444418491 1107 58-59 -6.76432068689277 1107 60-61 -9.240350964669009 1107 62-63 -2.9278686195629398 1107 64-65 -0.37202148485452113 1107 66-67 -1.7673689715088194 1107 68-69 -4.109655607942804 1107 70-71 -4.998288123069223 1107 72-73 -6.481058867262094 1107 74-75 -6.7553120480358295 1107 76-77 -9.092790078297098 1107 78-79 -12.532382470840819 1107 80-81 -8.490062780283203 1107 82-83 -4.581046682951637 1107 84-85 -3.585362543293936 1107 86-87 -3.2677295821178447 1107 88-89 -3.4974862096908623 1107 90-91 -8.766510853417639 1107 92-93 -11.779889630089901 1107 94-95 -11.931632117757882 1107 96-97 -12.33699445007005 1107 98-99 -11.551440863679655 1107 100-101 -11.135076905562538 1108 1 0.05438195953224323 1108 2 -0.014586533023880577 1108 3 0.01672716472966229 1108 4 -0.028273597112089988 1108 5 -0.16253833125566786 1108 6 -0.01889480844356939 1108 7 0.002253510634680822 1108 8 0.009660006181185565 1108 9 0.09503264786663834 1108 10-11 0.09668443883779787 1108 12-13 -0.0657342090648001 1108 14-15 0.12221300600325691 1108 16-17 0.08623744369810282 1108 18-19 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3.2620581770138415 2312 82-83 1.8608702386814002 2312 84-85 1.428106363322673 2312 86-87 1.2397934211670005 2312 88-89 1.4728255688168836 2312 90-91 3.068694852823562 2312 92-93 5.133637540252661 2312 94-95 9.132318027713726 2312 96-97 9.807404911731922 2312 98-99 10.24206498334052 2312 100-101 9.304311400397106 2313 1 -0.37060875876394306 2313 2 -0.3461813461881391 2313 3 -0.34766506333347635 2313 4 -0.4030358500806841 2313 5 -0.41424395762086874 2313 6 -0.25662222084955033 2313 7 -0.36155814445571366 2313 8 -0.4221572693247353 2313 9 -0.4686210743151733 2313 10-11 -0.47524711304815526 2313 12-13 -0.5088628480418151 2313 14-15 -0.5205262705633729 2313 16-17 -0.5410978830957021 2313 18-19 -0.6258663038447025 2313 20-21 -0.6047817700634255 2313 22-23 -0.5713968370632472 2313 24-25 -0.6231559484692042 2313 26-27 -0.6134843346932186 2313 28-29 -0.7427726158599981 2313 30-31 -0.6463009280310104 2313 32-33 -0.49438742959817716 2313 34-35 -0.6329480374467451 2313 36-37 -0.7379723463711301 2313 38-39 -1.055905347342545 2313 40-41 -1.2813743433739475 2313 42-43 -0.9674810130283831 2313 44-45 -0.8071824680818196 2313 46-47 -0.6966238606037223 2313 48-49 -0.8361281904759252 2313 50-51 -0.6343728648082347 2313 52-53 -0.8314360525646194 2313 54-55 -0.794029779169307 2313 56-57 0.35613210159034026 2313 58-59 3.9506448775998315 2313 60-61 3.594139213885814 2313 62-63 1.0535446373222577 2313 64-65 0.9722943314516321 2313 66-67 1.5985476667245813 2313 68-69 -0.08449239488905391 2313 70-71 2.548713745141285 2313 72-73 8.08379337579493 2313 74-75 9.70392981585709 2313 76-77 8.429990533659996 2313 78-79 6.255724282128821 2313 80-81 3.2781832972217693 2313 82-83 1.8257875616183696 2313 84-85 1.2602151612434014 2313 86-87 1.2235069494321067 2313 88-89 1.2909064005257846 2313 90-91 2.6657826760854206 2313 92-93 4.69801596780302 2313 94-95 8.538922680085395 2313 96-97 9.196542169691636 2313 98-99 9.53184650380836 2313 100-101 8.85380644913005 2314 1 -0.3246736694865362 2314 2 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3.6344660667588506 2314 62-63 1.2227826010537477 2314 64-65 1.2724730504942627 2314 66-67 1.8696400089673375 2314 68-69 -0.26896158796246894 2314 70-71 2.8209943542514964 2314 72-73 8.602972405195558 2314 74-75 10.113029348130322 2314 76-77 9.081892560756735 2314 78-79 6.553400555575692 2314 80-81 3.643941987772102 2314 82-83 2.2252585212478273 2314 84-85 1.646374205189165 2314 86-87 1.7524100381469125 2314 88-89 1.8237243374494163 2314 90-91 3.158128371942162 2314 92-93 5.206651668574114 2314 94-95 9.162954916890655 2314 96-97 9.937138469735807 2314 98-99 10.204730647630868 2314 100-101 9.227823104868913 2315 1 -0.7930213482419219 2315 2 -0.5514235609997336 2315 3 -0.6132193300839148 2315 4 -0.3984626056749079 2315 5 -0.6797310991048207 2315 6 -0.7485705451343989 2315 7 -0.6788075926860273 2315 8 -0.7797839078524902 2315 9 -1.107108554110468 2315 10-11 -0.9026883918650341 2315 12-13 -0.6884809992508778 2315 14-15 -0.8129846797482543 2315 16-17 -0.8988037294379154 2315 18-19 -0.8872205426710309 2315 20-21 -0.9305890185181909 2315 22-23 -1.009666092617195 2315 24-25 -0.8324243367647028 2315 26-27 -0.8998004463681823 2315 28-29 -0.8587325205958294 2315 30-31 -0.8720828031350507 2315 32-33 -0.7099931143027476 2315 34-35 -0.8048263098034809 2315 36-37 -1.0338440753569031 2315 38-39 -0.9933771547306236 2315 40-41 -0.780380889429928 2315 42-43 -0.697216230702189 2315 44-45 -0.6120634147091835 2315 46-47 -0.5667968826906176 2315 48-49 -0.5462124863855848 2315 50-51 -0.5134336078043873 2315 52-53 -0.5886355730217119 2315 54-55 -0.4558755091968578 2315 56-57 0.9006603183673576 2315 58-59 4.253241215782285 2315 60-61 3.876290202775241 2315 62-63 1.4898095324188922 2315 64-65 1.4508082049017048 2315 66-67 2.111296097335849 2315 68-69 6.2432588012831935 2315 70-71 4.986433916015365 2315 72-73 9.295637024939214 2315 74-75 10.362387062432362 2315 76-77 9.074062500000707 2315 78-79 6.624291667841337 2315 80-81 3.5744879841763755 2315 82-83 1.8073851698108783 2315 84-85 1.535804655333692 2315 86-87 1.4646433936187897 2315 88-89 1.5249779104146768 2315 90-91 3.014959921694082 2315 92-93 4.905098421795362 2315 94-95 8.764351140661386 2315 96-97 9.528086950299414 2315 98-99 9.63116806873997 2315 100-101 8.750221758523827 2316 1 -0.4850884099286965 2316 2 -0.5415867576240032 2316 3 -0.658171500544551 2316 4 -0.44214593886491116 2316 5 -0.7347694688012112 2316 6 -0.7242096578584594 2316 7 -0.6073689369010467 2316 8 -0.6464004803507208 2316 9 -0.7579443169034761 2316 10-11 -0.6297097524771118 2316 12-13 -0.5927357427887969 2316 14-15 -0.8206204571610201 2316 16-17 -0.9866888955441624 2316 18-19 -1.0031656096495851 2316 20-21 -0.8427335935849172 2316 22-23 -0.884191846323013 2316 24-25 -2.1991573656630976 2316 26-27 -1.4703565418036746 2316 28-29 -1.1899755511281853 2316 30-31 -0.8046344811105328 2316 32-33 -0.7471935884415402 2316 34-35 -0.7613418483122132 2316 36-37 -0.9071432744235253 2316 38-39 -1.1409770420724925 2316 40-41 -0.9336281955246548 2316 42-43 -1.0340832095436738 2316 44-45 -0.9165861512984179 2316 46-47 -0.8907305720580467 2316 48-49 -1.1667195984675232 2316 50-51 -1.1098055788630035 2316 52-53 -0.8394556052425131 2316 54-55 -0.8125868377887997 2316 56-57 0.378960011925777 2316 58-59 3.808519127335618 2316 60-61 3.8013456718544525 2316 62-63 1.3360052064040353 2316 64-65 1.107156117859688 2316 66-67 6.153102870010088 2316 68-69 8.75380652619096 2316 70-71 5.4873496216479865 2316 72-73 9.416547255329395 2316 74-75 10.379297248389943 2316 76-77 8.970678062015708 2316 78-79 6.217107777093926 2316 80-81 3.3116940840551194 2316 82-83 1.8972999022263153 2316 84-85 1.521621340776715 2316 86-87 1.389132526265854 2316 88-89 1.5022812746788325 2316 90-91 2.9236360668367425 2316 92-93 4.753222972094495 2316 94-95 8.90729420530651 2316 96-97 9.574679722692725 2316 98-99 9.845040605955852 2316 100-101 8.877718046252115 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 12.0 4 39.0 5 108.0 6 218.0 7 602.0 8 1442.0 9 2390.0 10 3877.0 11 5919.0 12 8155.0 13 11610.0 14 15222.0 15 21511.0 16 28829.0 17 37641.0 18 43525.0 19 46517.0 20 49361.0 21 48990.0 22 47495.0 23 45699.0 24 47452.0 25 51877.0 26 69144.0 27 99566.0 28 138227.0 29 179177.0 30 211705.0 31 228888.0 32 238361.0 33 247919.0 34 249308.0 35 236025.0 36 192017.0 37 113043.0 38 33543.0 39 1369.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.67990052924566 19.60429390313502 14.777807166204616 23.93799840141471 2 15.988771866691673 20.450061251948473 39.25337556821747 24.30779131314238 3 19.468071831124885 24.101167778145367 29.627799736495387 26.802960654234358 4 13.604890909275577 16.92487779256149 36.212215085091785 33.25801621307116 5 14.12846214489286 37.11654526685938 34.238847164006614 14.516145424241147 6 32.96269641531842 36.144626387394595 16.886584595249683 14.0060926020373 7 29.21254735583585 31.800160186419845 20.50806953599624 18.479222921748065 8 26.31793919366218 35.92830053848958 19.127026590756987 18.626733677091252 9 26.09540523246619 16.601639299068335 19.592075995687736 37.71087947277774 10-11 25.321896351841346 26.234081111786615 27.338246539088246 21.105775997283796 12-13 25.942364810061562 23.324336147719738 27.651654751834382 23.081644290384318 14-15 22.9118867897836 24.901107559064315 25.29450087293777 26.892504778214317 16-17 22.84530040032095 28.038771933133777 26.03947057227469 23.076457094270577 18-19 22.892800256533867 27.018218185102683 27.700851473346706 22.388130085016748 20-21 23.502315012978872 26.118656929731266 27.828309612491058 22.5507184447988 22-23 23.28929536837798 25.985930002597225 27.629364318184784 23.095410310840006 24-25 23.145033277858147 26.193345298949357 27.66054190601526 23.001079517177235 26-27 22.96725097069629 26.43201280912832 27.635171997515943 22.965564222659445 28-29 22.886174828768084 26.498609123287885 27.698878501926842 22.91633754601719 30-31 23.100192361934287 26.369583415335807 27.531264482695132 22.99895974003477 32-33 23.00710357993876 26.324670929370836 27.443422196701295 23.22480329398911 34-35 23.230822052098603 26.375317923782447 27.557160986646068 22.836699037472883 36-37 23.34030999299915 26.161729940682367 27.50769249748149 22.990267568836988 38-39 22.988609590714823 26.46425637065245 27.452470117115112 23.09466392151762 40-41 23.243117231345025 26.27760386855547 27.54433626897745 22.93494263112206 42-43 23.032754705808507 26.203692079496033 27.471715077527282 23.291838137168178 44-45 23.05610591113031 26.328383791782905 27.478799795286474 23.136710501800316 46-47 23.211548499098964 26.362298618520462 27.158127492634694 23.26802538974588 48-49 22.987440153410464 26.48913917064737 27.186071868519836 23.337348807422337 50-51 22.930392616981514 26.56568128576941 27.193377909469095 23.310548187779982 52-53 23.1896630386729 26.479855555089937 27.000335424794912 23.330145981442246 54-55 23.010031382081376 26.520561612277106 27.174778239338256 23.294628766303262 56-57 22.91371914697289 26.44749138789919 27.338354523385043 23.30043494174287 58-59 21.41574369901271 26.15795098713198 29.07733333528306 23.348971978572248 60-61 22.91044135262944 26.400086165866384 27.46387801707889 23.225594464425285 62-63 22.793469111478572 26.454248285996346 27.25257198035459 23.499710622170493 64-65 22.148572288022972 26.38469788046586 27.814262660639848 23.65246717087132 66-67 22.15029797509451 26.00096809697166 28.5441919703166 23.304541957617232 68-69 22.607689959783013 25.246701259196957 29.35166618034136 22.793942600678665 70-71 21.7390271756626 25.547850336715488 29.511120099674095 23.20200238794782 72-73 21.659927038937717 25.735306082899275 29.279065707856923 23.32570117030609 74-75 22.202623099845116 25.91856140721453 28.530141392000942 23.348674100939412 76-77 20.749189970681158 26.246014068640584 28.996572741183247 24.00822321949501 78-79 21.877583899366122 26.464120835271114 27.644441966997864 24.013853298364904 80-81 22.457417773753765 26.624537867312053 26.77048691518813 24.147557443746045 82-83 22.672694949149065 26.515144645044604 26.68066365760381 24.131496748202526 84-85 22.587567082180648 26.548700815242675 26.56366388909696 24.300068213479715 86-87 22.548918428566843 26.50837149330188 26.657607547267006 24.285102530864275 88-89 22.62430045400662 26.452215404303846 26.517363685564504 24.406120456125027 90-91 22.21369215137941 26.454201851693966 26.870668471737112 24.461437525189513 92-93 22.12843500600426 26.378985510011372 26.963421895478103 24.529157588506273 94-95 20.629752127486327 26.244224291410763 28.504052416175256 24.621971164927658 96-97 20.3976025198793 26.16587514585443 28.7102710369912 24.726251297275077 98-99 20.883833422285825 26.20008885474156 27.906547098878026 25.009530624094587 100-101 20.861993036070157 26.333985237984052 27.719267443245503 25.08475428270029 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 111.0 1 85.0 2 72.5 3 104.5 4 116.0 5 124.5 6 175.5 7 277.5 8 418.5 9 552.5 10 644.5 11 768.0 12 941.5 13 1107.0 14 1273.5 15 1423.0 16 1599.0 17 1848.0 18 2091.5 19 2383.0 20 2762.0 21 3194.0 22 3845.5 23 4814.5 24 5999.5 25 7593.0 26 9888.0 27 12670.0 28 15682.0 29 19259.5 30 23450.0 31 28333.0 32 34303.5 33 40716.0 34 47029.5 35 53235.5 36 59950.5 37 66856.0 38 72674.5 39 77874.0 40 82969.0 41 87636.0 42 92273.0 43 97435.5 44 102407.5 45 106672.0 46 110313.5 47 113250.5 48 115381.0 49 116179.5 50 115101.5 51 112978.5 52 110688.0 53 107470.5 54 102868.0 55 95925.5 56 87583.5 57 78839.5 58 69108.0 59 58584.5 60 47595.0 61 37456.0 62 28578.0 63 21097.0 64 15452.0 65 11240.5 66 8029.5 67 5658.0 68 3893.5 69 2610.5 70 1775.0 71 1196.0 72 784.0 73 572.0 74 381.0 75 233.5 76 155.5 77 87.5 78 54.0 79 33.0 80 18.0 81 11.5 82 6.0 83 3.5 84 4.0 85 3.0 86 1.5 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021800733317179268 2 0.005114647916343223 3 0.003627409869746966 4 0.0 5 0.061811064180488304 6 7.254819739493933E-5 7 0.0 8 1.08822296092409E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.8137049348734832E-4 16-17 0.0 18-19 0.0013421416518063776 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.4509639478987865E-4 28-29 0.0026661462542640203 30-31 0.009630773204178196 32-33 3.26466888277227E-4 34-35 0.0012514564050627034 36-37 0.02515608744669521 38-39 0.023705123498796426 40-41 0.026135488111526897 42-43 0.026334995654362973 44-45 0.026897244184173753 46-47 0.028783497316442176 48-49 0.028402619280118745 50-51 0.024648250064930637 52-53 0.02109338839257861 54-55 0.015888055229491712 56-57 0.14946742368292376 58-59 0.7749417075233932 60-61 0.3119572487982391 62-63 4.1715213502090115E-4 64-65 0.15242376272676753 66-67 1.455244291545088 68-69 2.7867939065318046 70-71 2.249193626785955 72-73 1.918174339122196 74-75 1.5990166817325089 76-77 1.145463488668697 78-79 0.43625044798511886 80-81 1.4509639478987865E-4 82-83 1.8137049348734832E-4 84-85 5.44111480462045E-5 86-87 0.0029563390438437777 88-89 0.0 90-91 0.23170080543008748 92-93 0.18106216364841984 94-95 1.3261447742807935 96-97 1.3124513020224986 98-99 1.0425175965652782 100-101 0.4171158609222037 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2756788.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.49252336842925 #Duplication Level Percentage of deduplicated Percentage of total 1 71.61726929721942 36.87753912870161 2 14.75444007763192 15.194867009711011 3 5.523039799194636 8.531857677743835 4 2.700364501200013 5.561943287252738 5 1.503554403111333 3.87109051189575 6 0.9195474487603833 2.840989109620412 7 0.605827106157519 2.1836896494730738 8 0.42459353467034855 1.7490714004877517 9 0.2943838031602335 1.3642708377163855 >10 1.515351859834536 13.885273837243197 >50 0.09756287179491785 3.400078997280709 >100 0.04300659392622569 3.783019535490675 >500 6.352220030936372E-4 0.20174297720593998 >1k 4.2348133536898407E-4 0.5545660401768816 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4993 0.18111657479646603 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4259 0.1544913863525233 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2878 0.10439685605131768 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.627409869746966E-5 0.0 0.0 0.0 0.0 7 7.254819739493933E-5 0.0 0.0 0.0 0.0 8 7.254819739493933E-5 0.0 0.0 0.0 0.0 9 7.254819739493933E-5 0.0 0.0 0.0 3.627409869746966E-5 10-11 7.254819739493933E-5 0.0 0.0 3.627409869746966E-5 1.08822296092409E-4 12-13 1.0882229609240898E-4 0.0 0.0 5.441114804620449E-5 1.4509639478987865E-4 14-15 1.8137049348734832E-4 0.0 0.0 7.254819739493933E-5 1.4509639478987865E-4 16-17 1.9950754283608316E-4 0.0 0.0 1.08822296092409E-4 1.8137049348734832E-4 18-19 2.17644592184818E-4 0.0 0.0 1.08822296092409E-4 2.17644592184818E-4 20-21 2.17644592184818E-4 0.0 0.0 2.1764459218481797E-4 2.357816415335528E-4 22-23 2.901927895797573E-4 0.0 0.0 3.990150856721663E-4 2.5391869088228764E-4 24-25 3.6274098697469665E-4 0.0 0.0 8.161672206930675E-4 2.5391869088228764E-4 26-27 3.990150856721663E-4 0.0 0.0 0.0016141973920374 2.7205574023102247E-4 28-29 4.715632830671056E-4 0.0 0.0 0.0055680741500615935 3.0832983892849214E-4 30-31 5.078373817645753E-4 0.0 0.0 0.022018377909364086 3.26466888277227E-4 32-33 5.078373817645753E-4 0.0 0.0 0.060160592689753434 3.26466888277227E-4 34-35 5.441114804620449E-4 0.0 0.0 0.11361047712047498 3.26466888277227E-4 36-37 7.254819739493933E-4 0.0 0.0 0.20202859269555729 3.26466888277227E-4 38-39 7.61756072646863E-4 0.0 0.0 0.3394348785615724 3.26466888277227E-4 40-41 7.61756072646863E-4 0.0 0.0 0.4880498609251056 3.26466888277227E-4 42-43 8.161672206930675E-4 0.0 0.0 0.6362839652523153 3.26466888277227E-4 44-45 9.431265661342113E-4 0.0 0.0 0.8157319315086977 3.26466888277227E-4 46-47 9.794006648316809E-4 0.0 0.0 1.0249246586970053 3.26466888277227E-4 48-49 0.0011244970596215596 0.0 0.0 1.231034087496028 3.26466888277227E-4 50-51 0.0011244970596215596 0.0 0.0 1.4369984199002608 3.6274098697469665E-4 52-53 0.0011426341089702944 0.0 0.0 1.6358530289597895 3.6274098697469665E-4 54-55 0.0011607711583190292 0.0 0.0 1.8525363575291245 3.6274098697469665E-4 56-57 0.001197045257016499 3.627409869746966E-5 0.0 2.046385140968402 3.6274098697469665E-4 58-59 0.001197045257016499 3.627409869746966E-5 0.0 2.17802384514152 3.6274098697469665E-4 60-61 0.001197045257016499 3.627409869746966E-5 0.0 2.2746217699728817 3.6274098697469665E-4 62-63 0.001197045257016499 3.627409869746966E-5 0.0 2.35979335371454 3.6274098697469665E-4 64-65 0.001197045257016499 3.627409869746966E-5 0.0 2.4489369512635717 3.6274098697469665E-4 66-67 0.001197045257016499 3.627409869746966E-5 0.0 2.52623705558788 3.808780363234315E-4 68-69 0.0012695934544114382 3.627409869746966E-5 0.0 2.6129321514748325 3.990150856721663E-4 70-71 0.0012695934544114382 3.627409869746966E-5 0.0 2.7707970290062205 3.990150856721663E-4 72-73 0.001287730503760173 3.627409869746966E-5 0.0 2.9712476984084377 4.534262337183708E-4 74-75 0.001305867553108908 3.627409869746966E-5 0.0 3.2109832167000145 4.7156328306710566E-4 76-77 0.001305867553108908 3.627409869746966E-5 0.0 3.5494931057448014 4.7156328306710566E-4 78-79 0.001305867553108908 3.627409869746966E-5 0.0 4.017646623534345 4.7156328306710566E-4 80-81 0.001305867553108908 3.627409869746966E-5 0.0 4.537418183770388 4.897003324158404E-4 82-83 0.001305867553108908 3.627409869746966E-5 0.0 5.040884536641919 6.347967272057191E-4 84-85 0.0013784157505038472 3.627409869746966E-5 0.0 5.450038232900027 6.52933776554454E-4 86-87 0.0014509639478987866 3.627409869746966E-5 0.0 5.82157568880886 6.52933776554454E-4 88-89 0.0014509639478987866 3.627409869746966E-5 0.0 6.202925288415359 6.52933776554454E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4510 0.0 46.825195 1 GTATCAA 7455 0.0 36.357857 1 TCAACGC 9010 0.0 29.602463 4 ATCAACG 9080 0.0 29.375317 3 CAACGCA 9330 0.0 28.637667 5 TATCAAC 9385 0.0 28.522121 2 AACGCAG 9680 0.0 27.635849 6 ACGCAGA 11155 0.0 23.77052 7 CGCAGAG 11290 0.0 23.152552 8 GTACATG 9475 0.0 22.835554 1 TACATGG 9440 0.0 21.96587 2 ACATGGG 9640 0.0 20.776093 3 GCAGAGT 12695 0.0 20.256283 9 GTACTTT 6175 0.0 19.526594 14-15 GAGTACT 6350 0.0 19.247543 12-13 GGACCGA 645 0.0 18.985126 6 AGAGTAC 10965 0.0 18.383785 10-11 CAGAGTA 12025 0.0 18.329922 10-11 CATGGGG 6225 0.0 16.956837 4 AGTACTT 6760 0.0 16.89572 12-13 >>END_MODULE