FastQCFastQC Report
Fri 27 May 2016
SRR939788_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939788_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2977570
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49140.16503390348505662No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38050.12778876735055766No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40650.045.939941
GTATCAA74900.041.512611
ATCAACG92150.033.2328833
TCAACGC93200.033.161324
TATCAAC93200.032.90842
CAACGCA94450.032.5719345
AACGCAG99400.031.0918646
ACGCAGA113800.026.537537
CGCAGAG113950.026.2549118
GTACATG102150.025.0507641
TACATGG102500.024.3235552
ACATGGG106350.023.1337783
GCAGAGT125750.021.8464019
AGAGTAC106000.019.61448310-11
GAGTACT61250.019.16178112-13
CAGAGTA116200.018.68211410-11
GTACTTT62700.018.49730714-15
GGACCGA8450.018.370676
AGTACTT65050.018.04241612-13
CATGGGA43650.017.2432084
Produced by FastQC (version 0.11.5)