FastQCFastQC Report
Fri 27 May 2016
SRR939788_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939788_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2977570
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53260.1788706898578371No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46700.15683930184680797No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30800.10344005346641726No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44200.047.6701971
GTATCAA76800.039.546221
TCAACGC95450.031.0599424
ATCAACG96450.030.688733
CAACGCA96950.030.6283155
TATCAAC97200.030.6060492
AACGCAG101150.029.450346
ACGCAGA116950.025.2282267
CGCAGAG119600.024.6692398
GTACATG98850.023.331671
TACATGG100900.022.4302042
GCAGAGT134000.021.4518349
ACATGGG101700.021.1753923
AGAGTAC113600.020.18899510-11
GAGTACT66900.019.42768312-13
GTACTTT67400.018.9668614-15
CAGAGTA126350.018.58345610-11
AGTACTT70600.018.1743612-13
CGTACAC4052.7284841E-1117.5684243
TGGTATC24850.017.3748472
Produced by FastQC (version 0.11.5)