FastQCFastQC Report
Fri 27 May 2016
SRR939787_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939787_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022903
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110190.5447122279219517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78510.38810560862285537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55370.27371554642016943No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20820.10292139563785313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67300.041.5926781
GTATCAA122450.041.147711
TATCAAC159100.032.86182
ATCAACG158500.032.660173
TCAACGC160250.032.3923344
CAACGCA162800.031.7108755
AACGCAG167050.031.0177216
GTACATG133200.028.0673831
TACATGG138300.027.9929032
ACGCAGA187900.027.2981137
CGCAGAG189300.026.9959628
ACATGGG142050.026.5885583
GCAGAGT191600.025.3098249
CATGGGG52750.024.6462634
CATGGGA86100.023.5865064
GAGTACT94500.023.14485512-13
AGTACTT96450.022.79989212-13
ATGGGAT33750.022.0729085
GTACTTT99100.022.04822214-15
AGAGTAC172300.021.24319510-11
Produced by FastQC (version 0.11.5)