FastQCFastQC Report
Fri 27 May 2016
SRR939787_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939787_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022903
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121180.5990400923820866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97490.4819311652610135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72670.35923620658034516No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22710.11226440417558331No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC21930.10840855938223433No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG20840.1030202634530672No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70150.046.044361
GTATCAA120400.038.067411
ATCAACG157200.029.1487463
TCAACGC158350.028.9653914
TATCAAC160150.028.6426722
CAACGCA161550.028.3916425
AACGCAG165200.027.8327396
GTACATG138900.025.15531
ACGCAGA188550.024.3109827
TACATGG142000.024.2360672
CGCAGAG188900.024.191128
ACATGGG143650.023.3001583
GAGTACT97800.022.80853312-13
AGTACTT99300.022.74864812-13
GCAGAGT198000.022.4844789
GTACTTT105350.021.12922314-15
CATGGGG48000.021.1145534
AGAGTAC181700.020.1197710-11
CATGGGA89100.020.0514684
AGTACAT105300.019.8784352
Produced by FastQC (version 0.11.5)