##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939787_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2022903 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.393870096588913 31.0 31.0 34.0 30.0 34.0 2 31.590231958724665 31.0 31.0 34.0 30.0 34.0 3 31.63921453475525 31.0 31.0 34.0 30.0 34.0 4 35.46440486765801 37.0 35.0 37.0 33.0 37.0 5 35.19907627800246 37.0 35.0 37.0 32.0 37.0 6 35.23574239595275 37.0 35.0 37.0 32.0 37.0 7 35.15409587113174 37.0 35.0 37.0 32.0 37.0 8 35.09344639856681 37.0 35.0 37.0 32.0 37.0 9 36.680021731145786 39.0 35.0 39.0 32.0 39.0 10-11 36.533181027463996 38.0 35.0 39.0 32.0 39.0 12-13 36.466590340713324 38.0 35.0 39.0 32.0 39.0 14-15 37.44712450374536 39.0 36.0 41.0 32.0 41.0 16-17 37.387880437173706 39.0 36.0 41.0 32.0 41.0 18-19 37.41221674988865 39.0 36.0 41.0 32.0 41.0 20-21 37.36517667925748 39.0 36.0 41.0 31.5 41.0 22-23 37.211887816667435 39.0 36.0 40.0 31.0 41.0 24-25 37.04248102850211 39.0 36.0 40.0 31.0 41.0 26-27 36.7393513183776 39.0 35.5 40.0 30.0 41.0 28-29 36.498485839409994 38.0 35.0 40.0 30.0 41.0 30-31 36.207816192867384 38.0 35.0 40.0 30.0 41.0 32-33 36.128595884231714 38.0 35.0 40.0 30.0 41.0 34-35 36.160522279120656 38.0 35.0 40.0 30.0 41.0 36-37 36.090626194137826 38.0 35.0 40.0 30.0 41.0 38-39 35.94297872908389 38.0 35.0 40.0 29.5 41.0 40-41 35.73524682102899 38.0 34.5 40.0 28.5 41.0 42-43 35.482884250999675 38.0 34.0 40.0 27.5 41.0 44-45 35.158293304226646 38.0 34.0 40.0 26.5 41.0 46-47 34.747673516723246 38.0 33.5 40.0 25.5 41.0 48-49 34.35808686822848 38.0 33.0 40.0 24.0 41.0 50-51 34.054097996789764 38.0 33.0 40.0 23.5 41.0 52-53 33.76073197775672 37.0 33.0 40.0 23.0 41.0 54-55 33.441883026521786 37.0 32.5 40.0 22.0 41.0 56-57 32.373663739685 36.5 31.0 39.5 14.0 41.0 58-59 29.335081316306315 34.5 24.5 38.5 7.0 40.0 60-61 29.312163262400617 34.0 24.5 38.0 6.5 40.0 62-63 30.85678255457627 35.0 29.0 38.0 4.5 40.0 64-65 30.305747729871378 35.0 29.0 38.0 2.0 40.0 66-67 24.995718282092618 28.5 17.5 36.0 2.0 39.0 68-69 17.334380590665987 13.0 7.0 29.0 2.0 35.5 70-71 16.212462238673826 13.0 7.5 25.5 2.0 33.0 72-73 18.36737599380692 18.5 7.0 30.0 2.0 34.5 74-75 19.122592630491923 18.0 7.5 30.5 2.0 35.0 76-77 20.20396158392172 23.5 8.5 31.0 2.0 34.5 78-79 23.341330998075538 29.0 12.0 34.0 2.0 36.0 80-81 25.85740295011674 31.5 22.0 34.5 2.0 36.0 82-83 26.615847373798942 33.0 25.0 35.0 2.0 36.0 84-85 26.55062032138961 33.0 25.0 35.0 2.0 36.0 86-87 26.23394596775031 33.0 24.5 35.0 2.0 35.5 88-89 25.79351852263801 32.5 23.0 35.0 2.0 35.0 90-91 24.33757847014909 31.5 10.5 34.0 2.0 35.0 92-93 22.4613426842513 30.5 2.0 34.0 2.0 35.0 94-95 18.05810634518808 23.0 2.0 33.0 2.0 35.0 96-97 16.745833339512572 15.0 2.0 32.5 2.0 34.5 98-99 15.647708763099367 2.0 2.0 32.0 2.0 34.0 100-101 15.020150496588318 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4131274023283744 1101 2 0.4217843325803976 1101 3 0.49265587370535613 1101 4 0.4338645653676423 1101 5 0.5048026656339246 1101 6 0.46232310166993074 1101 7 0.4896503424812195 1101 8 0.42661179918899705 1101 9 0.5917299544692298 1101 10-11 0.6075551294680537 1101 12-13 0.5925916153530082 1101 14-15 0.6419982926060825 1101 16-17 0.55941991301993 1101 18-19 0.6089020113312884 1101 20-21 0.6722030028489101 1101 22-23 0.7183886117013216 1101 24-25 0.767079875068255 1101 26-27 0.7326055512819636 1101 28-29 0.7234011488000007 1101 30-31 0.5922979555838168 1101 32-33 0.6646378250138625 1101 34-35 0.7807913304549245 1101 36-37 0.6586295229230359 1101 38-39 0.5963094873284405 1101 40-41 0.6110803998256031 1101 42-43 0.5086807771088431 1101 44-45 0.36874403544823053 1101 46-47 0.4235211944638948 1101 48-49 0.4657905689507089 1101 50-51 0.3822819055478348 1101 52-53 0.36211335394889943 1101 54-55 0.30667526658385214 1101 56-57 1.016553343722336 1101 58-59 -7.871803653255476 1101 60-61 -8.94584053931252 1101 62-63 -2.5976224374541452 1101 64-65 0.03437776843983187 1101 66-67 -0.9270105239842046 1101 68-69 -3.254524902348244 1101 70-71 -4.061418786304815 1101 72-73 -6.614095732986442 1101 74-75 -7.307785515139621 1101 76-77 -8.620149411285798 1101 78-79 -11.558186750062251 1101 80-81 -7.334961295239236 1101 82-83 -3.47953730278763 1101 84-85 -2.6317777002293035 1101 86-87 -2.376417639112372 1101 88-89 -2.4895773030998214 1101 90-91 -8.025978912550183 1101 92-93 -13.579278575734582 1101 94-95 -11.807597531856084 1101 96-97 -12.095017673343076 1101 98-99 -11.39492497324258 1101 100-101 -11.155162558164164 1102 1 0.4917886421051847 1102 2 0.6020036113031217 1102 3 0.6582558215496626 1102 4 0.46904211953538066 1102 5 0.5579435849229881 1102 6 0.541913364741454 1102 7 0.5051571069847753 1102 8 0.5875997898908949 1102 9 0.7477002077390722 1102 10-11 0.7950509142988409 1102 12-13 0.6699009098573327 1102 14-15 0.8365117721552124 1102 16-17 0.919349278024356 1102 18-19 0.881629079686725 1102 20-21 0.9301033540006074 1102 22-23 0.9046479468960769 1102 24-25 0.8046524883832404 1102 26-27 1.0867562003056221 1102 28-29 1.080360786587697 1102 30-31 1.1339446704495373 1102 32-33 0.7580853163699643 1102 34-35 0.8166388155163702 1102 36-37 0.8797564568426779 1102 38-39 0.7962810489868062 1102 40-41 0.8814385430829077 1102 42-43 1.008102736904 1102 44-45 1.0281842160148358 1102 46-47 1.0885835677278024 1102 48-49 1.044264374203145 1102 50-51 0.9731983282967107 1102 52-53 1.0253870904097724 1102 54-55 0.9282429947987296 1102 56-57 1.545999391804493 1102 58-59 -8.79840266364615 1102 60-61 -9.55494541721874 1102 62-63 -2.7528755499350943 1102 64-65 -0.06102640446528085 1102 66-67 -1.2709947468868457 1102 68-69 -3.673452304769885 1102 70-71 -4.054396876417265 1102 72-73 -6.64487574161911 1102 74-75 -7.519441806869796 1102 76-77 -8.660763409694152 1102 78-79 -11.498260251372887 1102 80-81 -7.568902062557399 1102 82-83 -3.774287225228516 1102 84-85 -2.9673116329034954 1102 86-87 -2.7234036245177684 1102 88-89 -2.8530611797965655 1102 90-91 -8.339796574446403 1102 92-93 -14.043924265471151 1102 94-95 -12.150363289844673 1102 96-97 -11.948882533894038 1102 98-99 -11.660610460920797 1102 100-101 -11.396547876336378 1103 1 0.19368594153353058 1103 2 0.24198662402329418 1103 3 0.13810879171466794 1103 4 0.16464687829281388 1103 5 0.2251213194112225 1103 6 0.2000603018131457 1103 7 0.19361561279987427 1103 8 0.24060213216429105 1103 9 0.17541237480578076 1103 10-11 0.25149086165566104 1103 12-13 0.20635369913389212 1103 14-15 0.3068389657174322 1103 16-17 0.24533111960782605 1103 18-19 0.260933876709025 1103 20-21 0.15200071140601779 1103 22-23 0.32155724757672743 1103 24-25 0.24840372039585645 1103 26-27 0.14552624587558682 1103 28-29 0.21855868549889124 1103 30-31 0.2282136376461139 1103 32-33 0.29101179565475377 1103 34-35 0.3291891106196232 1103 36-37 0.012809616012908975 1103 38-39 0.29003039674483233 1103 40-41 0.165300772185077 1103 42-43 0.1423452955478055 1103 44-45 0.08378610490758831 1103 46-47 0.15208367208652618 1103 48-49 0.17544864270652738 1103 50-51 0.14738215617440176 1103 52-53 0.15154228341542364 1103 54-55 0.054124246175682345 1103 56-57 0.6497210845028576 1103 58-59 -6.402321913191031 1103 60-61 -8.635712540744674 1103 62-63 -2.723839766491025 1103 64-65 -0.36316967158880686 1103 66-67 -1.9598515551335076 1103 68-69 -4.164961708649711 1103 70-71 -4.896949577568689 1103 72-73 -7.697469345589379 1103 74-75 -8.52339149437342 1103 76-77 -9.135231760014763 1103 78-79 -12.180653631551689 1103 80-81 -8.811245756392118 1103 82-83 -4.990712562365147 1103 84-85 -4.058840893927833 1103 86-87 -3.804434108858164 1103 88-89 -3.935262945777943 1103 90-91 -8.956098855264111 1103 92-93 -14.435356269970171 1103 94-95 -12.361656787137143 1103 96-97 -12.277263466397141 1103 98-99 -11.734743268981928 1103 100-101 -11.435465995328505 1104 1 0.09522293619409439 1104 2 0.159702108386373 1104 3 0.03283037828979829 1104 4 0.12986426959716368 1104 5 0.053721624521827493 1104 6 0.20822963819758655 1104 7 0.14374528480597348 1104 8 0.08988893613073401 1104 9 0.19813930081646447 1104 10-11 0.16479559086999984 1104 12-13 0.13034302026279931 1104 14-15 0.08297359577083085 1104 16-17 0.10981624546595015 1104 18-19 0.10371419707515628 1104 20-21 0.083633053129887 1104 22-23 0.25304847564689936 1104 24-25 0.28363636723033636 1104 26-27 0.28974249301517574 1104 28-29 0.16705982966364985 1104 30-31 0.24924338592986572 1104 32-33 0.18010218467077266 1104 34-35 0.11483747065013006 1104 36-37 0.1504507296666091 1104 38-39 0.1665120901086823 1104 40-41 0.13546667607523233 1104 42-43 0.11516184779799232 1104 44-45 0.10667512092589959 1104 46-47 0.06497268810483092 1104 48-49 0.11762064880873879 1104 50-51 -0.18095308410019584 1104 52-53 -0.06149356708039022 1104 54-55 -0.16138300020112695 1104 56-57 0.5735483156241372 1104 58-59 -6.4771312190644075 1104 60-61 -8.875558078502106 1104 62-63 -2.9608611242332046 1104 64-65 -0.8444339736483073 1104 66-67 -2.1371894651258785 1104 68-69 -4.325970480579535 1104 70-71 -4.910467884204845 1104 72-73 -7.697602831478012 1104 74-75 -8.681084095441307 1104 76-77 -9.173073102501416 1104 78-79 -12.179549512558555 1104 80-81 -8.586472682402796 1104 82-83 -4.937815918581773 1104 84-85 -4.0814190785197475 1104 86-87 -3.773915420833756 1104 88-89 -4.052732434717683 1104 90-91 -9.212784591343441 1104 92-93 -14.409728886293587 1104 94-95 -12.230419182255375 1104 96-97 -11.853297028563425 1104 98-99 -11.37272000429848 1104 100-101 -10.994720381293417 1105 1 0.12882542066614278 1105 2 0.15380148726836396 1105 3 0.05469373232706687 1105 4 0.11713135034250399 1105 5 0.09210671768952494 1105 6 0.08860230900504007 1105 7 0.048279446296653816 1105 8 0.08901937091334133 1105 9 0.17720762379782684 1105 10-11 0.13650366540416314 1105 12-13 0.14043618796466717 1105 14-15 0.17381210508759892 1105 16-17 0.13099636968955508 1105 18-19 0.25458376229254753 1105 20-21 0.16021690406157063 1105 22-23 0.3461540657090154 1105 24-25 0.2864935100874746 1105 26-27 0.2339660954996461 1105 28-29 0.1430846743841414 1105 30-31 0.051293075370857366 1105 32-33 0.09354938963971904 1105 34-35 0.20157660108492337 1105 36-37 -0.011102065364447355 1105 38-39 0.0020400404192386645 1105 40-41 0.14866543383920572 1105 42-43 -0.003471692574684937 1105 44-45 -0.1103745685150983 1105 46-47 -0.19269811934858438 1105 48-49 -0.06390109032169988 1105 50-51 -0.17141892260951153 1105 52-53 0.040618234161854616 1105 54-55 -0.028432689642116316 1105 56-57 0.5867470733881177 1105 58-59 -6.444056684902918 1105 60-61 -8.86282515924745 1105 62-63 -2.840302118022027 1105 64-65 -0.7205209301700464 1105 66-67 -2.0124068564302284 1105 68-69 -4.054417685548479 1105 70-71 -4.838200803459504 1105 72-73 -6.468410284894162 1105 74-75 -6.829158629602798 1105 76-77 -8.443663164613216 1105 78-79 -11.575729636782157 1105 80-81 -8.017777030228885 1105 82-83 -4.467536415476186 1105 84-85 -3.591201687215399 1105 86-87 -3.3547228742499016 1105 88-89 -3.4543473415499832 1105 90-91 -9.002287696933502 1105 92-93 -11.717089134740792 1105 94-95 -11.564984399646677 1105 96-97 -11.56646473042678 1105 98-99 -11.12799950740407 1105 100-101 -10.651273176324473 1106 1 0.18996473300532557 1106 2 0.18026857734213309 1106 3 0.1558722240752921 1106 4 0.19299481321146317 1106 5 0.15151560612927284 1106 6 0.18696827888975776 1106 7 0.19901600363930072 1106 8 0.2872454143371854 1106 9 0.22142438543310305 1106 10-11 0.2228363483385749 1106 12-13 0.29300422720626784 1106 14-15 0.3812200451368781 1106 16-17 0.35836715148978016 1106 18-19 0.30664068126735344 1106 20-21 0.2252018619670224 1106 22-23 0.307191153994836 1106 24-25 0.2678051651842992 1106 26-27 0.2964459810708959 1106 28-29 0.24687862489682288 1106 30-31 0.1543318116794481 1106 32-33 0.2847794987046157 1106 34-35 0.3544388745789604 1106 36-37 0.3709181617828392 1106 38-39 0.3395865854017117 1106 40-41 0.42242727900281096 1106 42-43 0.35482827866265865 1106 44-45 0.37140467417179934 1106 46-47 0.27937683459229135 1106 48-49 0.3195347261880741 1106 50-51 0.3640485483751803 1106 52-53 0.34691259279337316 1106 54-55 0.2844642545194489 1106 56-57 1.0046837022487267 1106 58-59 -6.490967808279187 1106 60-61 -8.678368656942549 1106 62-63 -2.2872408380058573 1106 64-65 0.3920926629082615 1106 66-67 -1.4263645209021192 1106 68-69 -3.922053083833603 1106 70-71 -4.603995391414946 1106 72-73 -6.2298414993781055 1106 74-75 -6.601282169120363 1106 76-77 -8.321810926955896 1106 78-79 -11.675644903536408 1106 80-81 -7.8552396193348315 1106 82-83 -4.112417050431372 1106 84-85 -3.074074299720163 1106 86-87 -2.9743844854253645 1106 88-89 -2.876763560581537 1106 90-91 -7.990603060081536 1106 92-93 -11.444277234772665 1106 94-95 -11.34943235089008 1106 96-97 -11.594464792616318 1106 98-99 -11.117993988082558 1106 100-101 -10.911735400066885 1107 1 0.08963478942418135 1107 2 0.09166506020184428 1107 3 -0.0011932077774545746 1107 4 -0.0014009505303462788 1107 5 0.11532066137847607 1107 6 0.10265544606143351 1107 7 0.12885514334429615 1107 8 0.16317867263440888 1107 9 0.05364070996085246 1107 10-11 0.289621568378422 1107 12-13 0.19213947414409915 1107 14-15 0.1356694394789315 1107 16-17 0.2329267606929193 1107 18-19 0.12853100830452746 1107 20-21 0.20299880134452053 1107 22-23 0.22259536048012762 1107 24-25 0.3321908313450379 1107 26-27 0.27871121821834066 1107 28-29 0.22781392712718684 1107 30-31 0.255130599654656 1107 32-33 0.21935178108087428 1107 34-35 0.2134421440843397 1107 36-37 0.11732434446398798 1107 38-39 0.21810291835723916 1107 40-41 0.09057858676558084 1107 42-43 0.21528908122344603 1107 44-45 0.3918568047850357 1107 46-47 0.17673712841456535 1107 48-49 0.06921927523498539 1107 50-51 0.11252367185787904 1107 52-53 0.12277819257157319 1107 54-55 -0.06374338545845859 1107 56-57 0.8283359862947819 1107 58-59 -6.890366915564805 1107 60-61 -9.389362221112993 1107 62-63 -2.9660032699633625 1107 64-65 -0.2904240685945396 1107 66-67 -1.4877892407572553 1107 68-69 -3.7773361193460566 1107 70-71 -4.554323877856854 1107 72-73 -6.039818334802499 1107 74-75 -6.842685047639847 1107 76-77 -8.93099180991926 1107 78-79 -12.08196817893336 1107 80-81 -8.0113825245239 1107 82-83 -3.944665865225822 1107 84-85 -3.0576763636594286 1107 86-87 -2.903658491400421 1107 88-89 -2.8421931291659277 1107 90-91 -8.074706773738711 1107 92-93 -11.37706806598002 1107 94-95 -11.41816797148568 1107 96-97 -11.93968825082517 1107 98-99 -11.311932525162026 1107 100-101 -10.988203704137973 1108 1 0.037485286031834875 1108 2 -0.00615388766623326 1108 3 -0.061750306051575166 1108 4 0.037562635604032835 1108 5 -0.28604001364643494 1108 6 -0.056624896560350635 1108 7 -0.004654123085018114 1108 8 0.09472601126405067 1108 9 0.09302457969600653 1108 10-11 0.12359059210356804 1108 12-13 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2312 82-83 1.7490882716871958 2312 84-85 1.3918421661814584 2312 86-87 1.1579760148116698 2312 88-89 1.1702195103624078 2312 90-91 2.78517954565622 2312 92-93 4.849886036615967 2312 94-95 8.618717817407003 2312 96-97 9.284010620459345 2312 98-99 9.845537864178842 2312 100-101 9.299718790406326 2313 1 -0.31288593469926695 2313 2 -0.30083449857531264 2313 3 -0.3587687436596205 2313 4 -0.3631638981178753 2313 5 -0.3122118576127946 2313 6 -0.2507078468146773 2313 7 -0.39766642130533114 2313 8 -0.48337553049043436 2313 9 -0.2841773876784899 2313 10-11 -0.4564584904050122 2313 12-13 -0.4763571881819786 2313 14-15 -0.5952305509890579 2313 16-17 -0.6427402467588692 2313 18-19 -0.6169745930227961 2313 20-21 -0.5496520735049231 2313 22-23 -0.48541991637685555 2313 24-25 -0.5625062975424981 2313 26-27 -0.5578637123674923 2313 28-29 -0.6995458407722452 2313 30-31 -0.541231240990868 2313 32-33 -0.4580355549023736 2313 34-35 -0.5761816029696121 2313 36-37 -0.7842896366797092 2313 38-39 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3.7247052563253256 2314 62-63 1.2324497976254527 2314 64-65 1.122449337273551 2314 66-67 2.004708039729941 2314 68-69 -0.5136594840159638 2314 70-71 2.362835193637677 2314 72-73 8.115916890619701 2314 74-75 9.667217114778328 2314 76-77 8.614903888384585 2314 78-79 6.424670568122753 2314 80-81 3.317039195678781 2314 82-83 1.9719976926538472 2314 84-85 1.6043757817820605 2314 86-87 1.537474464120674 2314 88-89 1.656733798660472 2314 90-91 2.8392597086714986 2314 92-93 4.876569485139253 2314 94-95 8.672248530280108 2314 96-97 9.305681008191392 2314 98-99 9.72795794281044 2314 100-101 8.847186835201429 2315 1 -0.7539564553433813 2315 2 -0.5032170352589596 2315 3 -0.5558613884273775 2315 4 -0.49518332724784386 2315 5 -0.790356550867692 2315 6 -0.6693131989629251 2315 7 -0.7088983799568211 2315 8 -0.7348023643316353 2315 9 -1.1466479704272743 2315 10-11 -0.8763294431277444 2315 12-13 -0.7002228965107165 2315 14-15 -0.8978662457025379 2315 16-17 -1.0550545630975918 2315 18-19 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0.9433497368044925 2315 86-87 0.9940890085869256 2315 88-89 1.1803436813797639 2315 90-91 2.5306268396702762 2315 92-93 4.569522969012002 2315 94-95 8.53987242127262 2315 96-97 9.105463122192297 2315 98-99 9.18816898820259 2315 100-101 8.53830464104796 2316 1 -0.44415821264754385 2316 2 -0.464710776412101 2316 3 -0.5886167813420649 2316 4 -0.40323633338601894 2316 5 -0.47124505272349637 2316 6 -0.5729660139763268 2316 7 -0.5012348802209985 2316 8 -0.6724373095055753 2316 9 -0.7369947836011832 2316 10-11 -0.7212937458484987 2316 12-13 -0.7530313053481166 2316 14-15 -0.7356256584341807 2316 16-17 -0.9199469249561361 2316 18-19 -0.7492106680470343 2316 20-21 -0.6812653918082248 2316 22-23 -0.8732836411414198 2316 24-25 -2.157779849843614 2316 26-27 -1.2692859900060895 2316 28-29 -1.0485633851887783 2316 30-31 -0.6422795432989474 2316 32-33 -0.6318872631235806 2316 34-35 -0.7950177340467874 2316 36-37 -0.7584623138116555 2316 38-39 -1.0541277861210858 2316 40-41 -0.7651640760669451 2316 42-43 -0.9284592248773507 2316 44-45 -0.9132197537170725 2316 46-47 -0.7912269310636901 2316 48-49 -1.393246932911346 2316 50-51 -1.4717544170103807 2316 52-53 -1.340190012653224 2316 54-55 -1.1838623718291785 2316 56-57 -0.024380913192107556 2316 58-59 3.2846170354579662 2316 60-61 3.3520779894128374 2316 62-63 0.8101730712602908 2316 64-65 0.6682147886493794 2316 66-67 5.73301324829843 2316 68-69 8.520899957376667 2316 70-71 5.5222590278865535 2316 72-73 9.131477222473116 2316 74-75 9.778455733184526 2316 76-77 8.331125167888938 2316 78-79 5.828801804483653 2316 80-81 2.9485154166133896 2316 82-83 1.4673102318466604 2316 84-85 1.157350359753817 2316 86-87 1.1260635540948414 2316 88-89 1.171348015932903 2316 90-91 2.72098127663498 2316 92-93 4.522878619260204 2316 94-95 8.440293686675126 2316 96-97 9.12084768739976 2316 98-99 9.429045308300692 2316 100-101 8.545170129655487 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 23.0 4 52.0 5 109.0 6 307.0 7 649.0 8 1463.0 9 2462.0 10 4088.0 11 6392.0 12 8834.0 13 11828.0 14 15485.0 15 21667.0 16 29035.0 17 36586.0 18 37470.0 19 34346.0 20 35626.0 21 34979.0 22 33678.0 23 32851.0 24 34395.0 25 37418.0 26 48790.0 27 69615.0 28 94906.0 29 122906.0 30 147587.0 31 161993.0 32 171192.0 33 178947.0 34 181758.0 35 173631.0 36 141705.0 37 84587.0 38 24703.0 39 835.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.88757938289503 19.94540355485059 14.950140323049569 22.216876739204817 2 16.014134946420487 21.57378825660425 38.92098430483076 23.491092492144507 3 19.06867870428732 26.310926550739683 30.1256411207099 24.494753624263097 4 12.88855669302977 17.777817324903864 36.74847484036555 32.58515114170081 5 13.396229308462512 38.452832223370415 33.175417342925364 14.975521125241709 6 31.010084532107367 36.84443126204953 17.421128083444557 14.724356122398538 7 28.23279218034676 33.837509757017514 20.169083737579115 17.760614325056615 8 27.14864798062188 34.178753275001235 19.476246972168667 19.196351772208217 9 27.53216540783221 16.41131581692251 19.784339634673536 36.272179140571744 10-11 25.454013365939936 27.044499909288778 26.561085726799554 20.94040099797173 12-13 26.505942202863903 24.529228539381275 27.312876593687392 21.65195266406743 14-15 23.14572954246828 25.49167086032019 26.384844910618334 24.977754686593194 16-17 22.00683868677836 29.126680814651024 26.533031984232565 22.33344851433806 18-19 21.811945421258006 26.812228434226427 30.020087741435397 21.35573840308017 20-21 23.11148878616523 25.785022811276665 29.70772696466415 21.395761437893956 22-23 22.400431459145594 26.09880453981234 29.645143637633637 21.85562036340843 24-25 22.157191916765164 26.332083149809954 29.89406807938888 21.616656854036005 26-27 21.974151459328553 26.735683112285113 29.782992185227197 21.507173243159137 28-29 21.60208141360532 26.639785530952274 30.15330799621921 21.6048250592232 30-31 21.7335288458401 26.40489183366144 30.089117711910408 21.77246160858805 32-33 21.682509056943378 26.51792937400869 29.728698294103673 22.07086327494426 34-35 22.278165508418187 26.464395847113174 29.586228954531208 21.671209689937427 36-37 22.32707939974698 26.156452754563247 29.517392993059417 21.999074852630354 38-39 21.813885402252616 26.43049399860414 29.783309916488022 21.972310682655223 40-41 21.85787891374434 26.193828002927273 30.001458691825395 21.946834391502996 42-43 22.031398690283137 26.239087238060954 29.387016214588456 22.34249785706745 44-45 21.78013665896352 26.59625625545935 29.263447398759894 22.360159686817234 46-47 21.988118473084345 26.773662054918685 28.788967407885778 22.449252064111192 48-49 22.080165236742825 26.745324221972837 28.645972020617013 22.528538520667325 50-51 21.91036320105854 26.905453207713983 28.371973777767668 22.81220981345981 52-53 22.267120500496173 26.949902421441475 28.201711469077708 22.581265608984644 54-55 21.69431994009618 26.818998845778562 28.413747719182798 23.072933494942465 56-57 21.985408282898696 27.003687537627936 28.300638288602297 22.710265890871067 58-59 20.666996021581966 26.301531151568785 30.119905618142123 22.91156720870713 60-61 22.14684821888624 26.561732102200736 28.75120941966205 22.540210259250976 62-63 21.812400282268378 26.768607900301422 28.38883334425002 23.03015847318018 64-65 21.16440387375492 26.534310238042547 28.819035482355364 23.482250405847168 66-67 21.63819174147722 26.455437565826678 29.06557353194278 22.840797160753326 68-69 22.20259738145323 25.49324477818263 29.6669082531769 22.63724958718725 70-71 21.401433560286307 26.07174884489242 29.791138335911484 22.73567925890979 72-73 21.24008555443382 26.11310880966155 29.71229359740766 22.934512038496973 74-75 21.588651199252258 26.230785079322004 29.01589957738481 23.164664144040927 76-77 20.0001050304851 26.829912382068894 29.72175173844208 23.448230849003924 78-79 21.172799678663253 26.89186884475915 28.394808266675735 23.54052320990186 80-81 21.373636178933126 27.54269734406182 27.567340171367753 23.5163263056373 82-83 21.70267395751793 27.32905053906507 27.178573538342594 23.789701965074407 84-85 21.555828430725548 26.99064166695091 27.164031097882596 24.289498804440946 86-87 21.703084497722635 26.780842690838607 27.457835920411956 24.058236891026805 88-89 21.698975036852996 26.840919629324606 27.271415026531194 24.188690307291207 90-91 21.360018253948397 27.00144956368158 27.69045683677011 23.948075345599914 92-93 21.369399868614554 26.700662795756543 27.722775219208916 24.207162116419987 94-95 20.147440029522077 26.428046574935443 28.901247097333922 24.523266298208558 96-97 19.899767968884834 26.51301796506483 29.050180392105784 24.537033673944556 98-99 20.288916419804277 26.553409698320003 28.29812317975412 24.859550702121602 100-101 20.113474409805495 26.60684261761075 28.4177222416098 24.861960730973962 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 671.0 1 497.5 2 353.5 3 423.0 4 472.0 5 531.5 6 751.5 7 1231.0 8 1795.0 9 2196.5 10 2397.0 11 2588.0 12 2926.5 13 3251.5 14 3518.5 15 3820.5 16 4074.5 17 4279.0 18 4480.0 19 4661.5 20 5035.0 21 5557.5 22 6189.5 23 7067.5 24 8032.5 25 9419.0 26 11244.0 27 13299.0 28 15512.0 29 18127.0 30 21445.5 31 25144.0 32 29417.5 33 34087.0 34 38979.5 35 43863.0 36 48324.0 37 52326.5 38 55288.5 39 57528.0 40 60024.5 41 63559.0 42 67659.5 43 70886.5 44 72923.0 45 74841.5 46 76695.0 47 78386.0 48 80540.0 49 80465.5 50 78558.0 51 76178.0 52 73050.0 53 70015.5 54 66177.0 55 60584.0 56 54202.0 57 48332.0 58 42513.5 59 36575.5 60 30825.0 61 25248.5 62 20289.5 63 15934.5 64 12145.5 65 9343.5 66 7034.0 67 5203.5 68 3708.0 69 2550.0 70 1974.5 71 1596.0 72 1303.5 73 1059.5 74 786.0 75 519.5 76 305.5 77 176.0 78 106.5 79 59.0 80 36.0 81 29.0 82 16.0 83 7.5 84 6.0 85 3.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02110827854820523 2 0.00568489937480937 3 0.0038558447933489644 4 0.0 5 0.06742784997599983 6 1.4830172282111401E-4 7 0.0 8 1.4830172282111401E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.4716953803519E-4 16-17 0.0 18-19 0.0013594324591935452 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.7301867662463302E-4 28-29 0.002323393657530786 30-31 0.00914527290730203 32-33 3.7075430705278505E-4 34-35 0.001038112059747798 36-37 0.02582921672467736 38-39 0.02464280294210845 40-41 0.026842611830621636 42-43 0.02755940349092369 44-45 0.028152610382208145 46-47 0.029858080194650954 48-49 0.029289590257170015 50-51 0.02456865208069789 52-53 0.02125658027102634 54-55 0.016189604741304948 56-57 0.15457982908720783 58-59 0.7884461093784526 60-61 0.31835436498932473 62-63 2.71886491838709E-4 64-65 0.15097115383189405 66-67 1.4820285500589994 68-69 2.838667993472747 70-71 2.2915335040780502 72-73 1.9462376594428898 74-75 1.6270182010704417 76-77 1.160881169289877 78-79 0.43583404641745055 80-81 9.8867815214076E-5 82-83 1.23584769017595E-4 84-85 0.0 86-87 0.0030401853178328372 88-89 4.9433907607038E-5 90-91 0.23280898787534549 92-93 0.18060678144231335 94-95 1.3400790843653898 96-97 1.3265341936810613 98-99 1.0436980913073934 100-101 0.41722218020340074 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2022903.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.03896564876019 #Duplication Level Percentage of deduplicated Percentage of total 1 73.0963547433238 32.92184210334149 2 13.790250161791828 12.421972066495035 3 5.117726285725785 6.91491295147682 4 2.5215197808432936 4.542665711682817 5 1.4196229118960662 3.196917378153992 6 0.8706773071113533 2.352864319568596 7 0.5648137616865185 1.7807039327382295 8 0.4011931780592776 1.4455460612102988 9 0.2947598483921019 1.1948110817729074 >10 1.6300345234749516 13.84915201475987 >50 0.16524711630904845 5.171606913251631 >100 0.12030320190100925 10.024078372198234 >500 0.005402379334784972 1.555941421739053 >1k 0.0017640422317665217 1.1802888764063173 >5k 2.2050527897081522E-4 0.8449575295944254 >10k+ 1.1025263948540761E-4 0.6017392656103224 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12118 0.5990400923820866 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9749 0.4819311652610135 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7267 0.35923620658034516 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2271 0.11226440417558331 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 2193 0.10840855938223433 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2084 0.1030202634530672 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.943390760703801E-5 0.0 14-15 0.0 0.0 0.0 7.415086141055701E-5 0.0 16-17 0.0 0.0 0.0 1.4830172282111401E-4 0.0 18-19 0.0 0.0 0.0 2.4716953803519E-4 0.0 20-21 0.0 0.0 0.0 4.44905168463342E-4 0.0 22-23 0.0 0.0 0.0 0.001087545967354836 0.0 24-25 0.0 0.0 0.0 0.0025705631955659763 0.0 26-27 0.0 0.0 0.0 0.005289428113953066 0.0 28-29 0.0 0.0 0.0 0.012506778624580616 0.0 30-31 0.0 0.0 0.0 0.03890448528673891 0.0 32-33 0.0 0.0 0.0 0.09236725636375051 0.0 34-35 0.0 0.0 0.0 0.16590019392921956 0.0 36-37 0.0 0.0 0.0 0.28624704199855355 0.0 38-39 0.0 0.0 0.0 0.47159947857114254 0.0 40-41 0.0 0.0 0.0 0.6835226404825145 0.0 42-43 0.0 0.0 0.0 0.8887475079131328 0.0 44-45 0.0 0.0 0.0 1.1287985632529094 0.0 46-47 0.0 0.0 0.0 1.4026376944420962 0.0 48-49 0.0 0.0 0.0 1.6907632249297173 0.0 50-51 0.0 0.0 0.0 1.9881081791860509 0.0 52-53 0.0 0.0 0.0 2.265284099138713 0.0 54-55 0.0 0.0 0.0 2.550591896892733 0.0 56-57 0.0 0.0 4.943390760703801E-5 2.8016172797212717 0.0 58-59 0.0 0.0 4.943390760703801E-5 2.97263388308782 0.0 60-61 0.0 0.0 4.943390760703801E-5 3.1073659982708017 2.4716953803519003E-5 62-63 2.4716953803519003E-5 0.0 4.943390760703801E-5 3.22430190671525 4.943390760703801E-5 64-65 4.943390760703801E-5 0.0 4.943390760703801E-5 3.3423253611270534 4.943390760703801E-5 66-67 4.943390760703801E-5 0.0 4.943390760703801E-5 3.4364475212108543 4.943390760703801E-5 68-69 4.943390760703801E-5 0.0 4.943390760703801E-5 3.5444358923784285 4.943390760703801E-5 70-71 4.943390760703801E-5 0.0 4.943390760703801E-5 3.749858495439475 4.943390760703801E-5 72-73 4.943390760703801E-5 0.0 4.943390760703801E-5 4.006519343735216 4.943390760703801E-5 74-75 4.943390760703801E-5 0.0 4.943390760703801E-5 4.309821083858198 4.943390760703801E-5 76-77 4.943390760703801E-5 0.0 4.943390760703801E-5 4.725659114648602 4.943390760703801E-5 78-79 4.943390760703801E-5 0.0 4.943390760703801E-5 5.2925177331785065 4.943390760703801E-5 80-81 4.943390760703801E-5 2.4716953803519003E-5 4.943390760703801E-5 5.944501540607731 4.943390760703801E-5 82-83 4.943390760703801E-5 4.943390760703801E-5 4.943390760703801E-5 6.566157645720037 4.943390760703801E-5 84-85 4.943390760703801E-5 4.943390760703801E-5 4.943390760703801E-5 7.059433892776866 4.943390760703801E-5 86-87 4.943390760703801E-5 4.943390760703801E-5 4.943390760703801E-5 7.492969262490589 4.943390760703801E-5 88-89 4.943390760703801E-5 4.943390760703801E-5 7.415086141055701E-5 7.935773489880631 4.943390760703801E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7015 0.0 46.04436 1 GTATCAA 12040 0.0 38.06741 1 ATCAACG 15720 0.0 29.148746 3 TCAACGC 15835 0.0 28.965391 4 TATCAAC 16015 0.0 28.642672 2 CAACGCA 16155 0.0 28.391642 5 AACGCAG 16520 0.0 27.832739 6 GTACATG 13890 0.0 25.1553 1 ACGCAGA 18855 0.0 24.310982 7 TACATGG 14200 0.0 24.236067 2 CGCAGAG 18890 0.0 24.19112 8 ACATGGG 14365 0.0 23.300158 3 GAGTACT 9780 0.0 22.808533 12-13 AGTACTT 9930 0.0 22.748648 12-13 GCAGAGT 19800 0.0 22.484478 9 GTACTTT 10535 0.0 21.129223 14-15 CATGGGG 4800 0.0 21.114553 4 AGAGTAC 18170 0.0 20.11977 10-11 CATGGGA 8910 0.0 20.051468 4 AGTACAT 10530 0.0 19.878435 2 >>END_MODULE