FastQCFastQC Report
Fri 27 May 2016
SRR939786_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939786_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2243792
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123480.5503183895833482No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89950.4008838608926317No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64610.2879500417150966No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22870.10192566868943288No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74050.044.977621
GTATCAA132150.040.8304821
TATCAAC169800.031.83322
ATCAACG170050.031.7608623
TCAACGC173200.031.3733654
CAACGCA175800.030.80375
AACGCAG179800.030.1982636
GTACATG150500.028.7567371
TACATGG155400.027.7295252
ACATGGG157900.026.0707173
ACGCAGA205400.026.0450887
CGCAGAG205750.025.680068
GCAGAGT208700.024.0996889
GAGTACT103750.023.89680712-13
CATGGGA100100.023.2177374
GTACTTT111650.022.23371114-15
AGTACTT106650.021.28402712-13
GACCGGT1554.2057138E-521.248697
CATGGGG54750.021.0527574
ATGGGAG37000.020.3453275
Produced by FastQC (version 0.11.5)