FastQCFastQC Report
Fri 27 May 2016
SRR939786_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939786_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2243792
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135730.6049134679150295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104170.46425871916826517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78950.35185970892132606No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24800.11052717898985288No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC23180.10330725842680605No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG23170.10326269101592303No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79600.043.4614941
GTATCAA133500.036.9693871
ATCAACG176250.027.885323
TCAACGC178950.027.4910954
TATCAAC178750.027.4495752
CAACGCA181700.027.0489145
GTACATG152300.026.61021
AACGCAG186350.026.3994186
TACATGG156900.025.5258752
ACATGGG157950.024.5384673
ACGCAGA212750.023.145837
CGCAGAG213800.023.009978
GAGTACT103800.022.94357312-13
AGTACTT105850.022.85777912-13
CATGGGG52650.021.9855044
CATGGGA99600.021.7671644
GCAGAGT222800.021.6759229
GTACTTT111850.021.44074214-15
AGAGTAC200200.020.54493710-11
ATAGCGT2851.9990694E-919.974756
Produced by FastQC (version 0.11.5)