FastQCFastQC Report
Fri 27 May 2016
SRR939785_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939785_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2626408
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103580.39437893883966235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71910.27379599818459277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49860.18984103003036848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63500.041.3173981
GTATCAA110300.039.357471
ATCAACG140550.031.6637133
TCAACGC141250.031.5739754
TATCAAC142400.031.4183442
CAACGCA143150.030.9891825
AACGCAG148100.030.0174886
GTACATG123800.029.4704821
TACATGG128750.028.5953922
ACATGGG127450.028.068643
ACGCAGA169250.025.9300257
CGCAGAG170450.025.5804628
CATGGGA70500.024.6982864
GCAGAGT173800.023.4221849
CATGGGG53250.022.720114
TAAGGTG11750.022.6120535
GTAAGGT10850.021.8640274
GAGTACT98750.021.50015812-13
AGAGTAC152000.021.14733110-11
ATGGGAT32300.021.0050335
Produced by FastQC (version 0.11.5)