FastQCFastQC Report
Fri 27 May 2016
SRR939785_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939785_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2626408
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115250.4388122485158437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87440.33292618663969953No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64960.24733400141942913No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63950.047.494721
GTATCAA111050.039.0298541
ATCAACG139300.030.9026363
TCAACGC140300.030.6464354
CAACGCA143200.029.992885
TATCAAC146250.029.5002652
AACGCAG146800.029.3054036
ACGCAGA167350.025.622337
GTACATG131000.025.381531
CGCAGAG168900.025.2760838
TACATGG135200.024.3084222
ACATGGG132800.023.6811963
GCAGAGT179750.023.3567149
GAGTACT97500.022.0133812-13
GGTAAGG10500.021.1049563
CATGGGG55900.020.9161954
GTACTTT102650.020.81695214-15
AGGTAAG10250.020.7009072
AGAGTAC161500.020.2629510-11
AGTACTT104700.020.11702712-13
Produced by FastQC (version 0.11.5)