##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939785_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2626408 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.4232308917731 31.0 31.0 34.0 30.0 34.0 2 31.635882924511346 31.0 31.0 34.0 30.0 34.0 3 31.685967298302472 31.0 31.0 34.0 30.0 34.0 4 35.50526803147112 37.0 35.0 37.0 33.0 37.0 5 35.24165895017073 37.0 35.0 37.0 33.0 37.0 6 35.263319712702675 37.0 35.0 37.0 32.0 37.0 7 35.18212593016774 37.0 35.0 37.0 32.0 37.0 8 35.115129484832515 37.0 35.0 37.0 32.0 37.0 9 36.72022016381309 39.0 35.0 39.0 32.0 39.0 10-11 36.57150240937432 38.5 35.0 39.0 32.0 39.0 12-13 36.51578848373901 38.0 35.0 39.0 32.0 39.0 14-15 37.53498523458656 39.0 36.0 41.0 32.0 41.0 16-17 37.46810510781265 39.0 36.0 41.0 32.0 41.0 18-19 37.467025496419446 39.0 36.0 41.0 32.0 41.0 20-21 37.394758354376016 39.0 36.0 41.0 32.0 41.0 22-23 37.23946660229484 39.0 36.0 40.0 31.0 41.0 24-25 37.071950930700794 39.0 36.0 40.0 31.0 41.0 26-27 36.771762993411535 39.0 35.5 40.0 30.0 41.0 28-29 36.54308298634484 38.0 35.0 40.0 30.0 41.0 30-31 36.26625946920662 38.0 35.0 40.0 30.0 41.0 32-33 36.18029319892416 38.0 35.0 40.0 30.0 41.0 34-35 36.22832895726788 38.0 35.0 40.0 30.0 41.0 36-37 36.17517365923345 38.0 35.0 40.0 30.0 41.0 38-39 36.01667505581768 38.0 35.0 40.0 29.5 41.0 40-41 35.826078431073924 38.0 35.0 40.0 29.0 41.0 42-43 35.58664133676108 38.0 34.0 40.0 27.5 41.0 44-45 35.30113828468387 38.0 34.0 40.0 27.0 41.0 46-47 34.94482673674463 38.0 34.0 40.0 26.0 41.0 48-49 34.59309825434586 38.0 33.0 40.0 24.5 41.0 50-51 34.290760422599995 38.0 33.0 40.0 24.0 41.0 52-53 33.97676674758834 37.5 33.0 40.0 23.5 41.0 54-55 33.640096093219334 37.0 33.0 40.0 23.0 41.0 56-57 32.2126853482018 36.5 30.5 39.5 14.5 41.0 58-59 28.708100569294643 34.0 23.0 38.0 7.0 40.0 60-61 28.677270248948375 33.5 23.0 38.0 6.5 40.0 62-63 30.647230171397588 34.5 29.0 38.0 4.0 40.0 64-65 30.221836249356535 35.0 29.0 38.0 2.0 40.0 66-67 24.764963212113273 28.0 16.5 35.5 2.0 39.0 68-69 17.152051966031173 13.0 7.0 29.0 2.0 35.5 70-71 16.038525811678916 12.5 7.5 25.5 2.0 33.0 72-73 18.094824376106075 17.0 7.0 30.0 2.0 34.0 74-75 18.78911330608192 17.5 7.5 30.5 2.0 35.0 76-77 19.708438864030263 22.5 7.5 30.5 2.0 34.5 78-79 22.72117755504857 27.5 10.5 33.5 2.0 35.5 80-81 25.3654064791152 31.5 20.0 34.5 2.0 36.0 82-83 26.431472375959864 32.5 25.0 35.0 2.0 36.0 84-85 26.447327871374135 33.0 25.0 35.0 2.0 36.0 86-87 26.177065977563274 33.0 24.0 35.0 2.0 35.0 88-89 25.73992806905858 33.0 22.0 35.0 2.0 35.0 90-91 24.050234388564153 31.5 10.0 34.5 2.0 35.0 92-93 21.861304298494368 30.5 2.0 34.0 2.0 35.0 94-95 17.502022914946956 20.0 2.0 33.0 2.0 35.0 96-97 16.20212624999619 12.0 2.0 32.5 2.0 34.5 98-99 15.12629758971188 2.0 2.0 31.5 2.0 34.0 100-101 14.501509666434156 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3435956764982109 1101 2 0.32196628221778667 1101 3 0.318641018107229 1101 4 0.20698055566130336 1101 5 0.20093139518756686 1101 6 0.36024661262162994 1101 7 0.29107411930169746 1101 8 0.27088106790784394 1101 9 0.40652649265405927 1101 10-11 0.5006620710210399 1101 12-13 0.5059188366159546 1101 14-15 0.4975831497982668 1101 16-17 0.32417756658472285 1101 18-19 0.4235533705189596 1101 20-21 0.532972152814402 1101 22-23 0.33597707861041215 1101 24-25 0.3576308922977063 1101 26-27 0.46531638712738044 1101 28-29 0.5338856356337587 1101 30-31 0.5808269810034332 1101 32-33 0.410571368469796 1101 34-35 0.6316218563274063 1101 36-37 0.5782912777687343 1101 38-39 0.5258530815049838 1101 40-41 0.4118056285223304 1101 42-43 0.7018603387916542 1101 44-45 0.38819688380154815 1101 46-47 0.2970408023419253 1101 48-49 0.38073628377915725 1101 50-51 0.35641046403758025 1101 52-53 0.5585879577186503 1101 54-55 0.40834433500588574 1101 56-57 1.0091710112030228 1101 58-59 -8.06004389996906 1101 60-61 -9.441923575889948 1101 62-63 -2.536752250110492 1101 64-65 -0.022110294336947334 1101 66-67 -1.303670844500079 1101 68-69 -3.490661970797646 1101 70-71 -3.9493884351640887 1101 72-73 -6.548123835093985 1101 74-75 -7.592776426715407 1101 76-77 -8.556752285502041 1101 78-79 -11.383845866185013 1101 80-81 -7.743380935561927 1101 82-83 -3.987549465081006 1101 84-85 -2.996640423487431 1101 86-87 -2.7918410694509674 1101 88-89 -3.1238755064492274 1101 90-91 -8.64615735073237 1101 92-93 -14.165534113219447 1101 94-95 -12.316291568712492 1101 96-97 -12.305805892193879 1101 98-99 -11.612171444596795 1101 100-101 -11.379005849236743 1102 1 0.4577384205470132 1102 2 0.47934824962291245 1102 3 0.44702843868856235 1102 4 0.30504506147220667 1102 5 0.44221104062997796 1102 6 0.4072597368405013 1102 7 0.3805849045299041 1102 8 0.48022273082318634 1102 9 0.6081655970969919 1102 10-11 0.6279610201333483 1102 12-13 0.6408710713457211 1102 14-15 0.6958971009073665 1102 16-17 0.7143938826497447 1102 18-19 0.7034664621401276 1102 20-21 0.7334950330585528 1102 22-23 0.6615295343271654 1102 24-25 0.8099461755378314 1102 26-27 0.8401603956187742 1102 28-29 0.8952426406344287 1102 30-31 0.8126692871706069 1102 32-33 0.7383040598213526 1102 34-35 0.586074334951924 1102 36-37 0.7340968666040553 1102 38-39 0.6461128329440058 1102 40-41 0.6735987747270045 1102 42-43 0.8407345281414749 1102 44-45 0.7633072479408796 1102 46-47 0.6416779636233869 1102 48-49 0.7087663762051548 1102 50-51 0.6876234182592214 1102 52-53 0.7094647323058112 1102 54-55 0.5895793032572456 1102 56-57 1.1552202219671273 1102 58-59 -8.819084822074675 1102 60-61 -9.316462186757636 1102 62-63 -2.6033040016605256 1102 64-65 0.130476683423705 1102 66-67 -0.8926510351660326 1102 68-69 -3.5684640439735986 1102 70-71 -4.074698595438454 1102 72-73 -6.688478091633634 1102 74-75 -7.476772368231467 1102 76-77 -8.608808880373806 1102 78-79 -11.524325245201222 1102 80-81 -7.674623547573592 1102 82-83 -3.4925375478706933 1102 84-85 -2.614255786646016 1102 86-87 -2.393560115867171 1102 88-89 -2.5411581426010628 1102 90-91 -8.260901482323584 1102 92-93 -14.079883074161948 1102 94-95 -12.131644543452333 1102 96-97 -12.129721054450918 1102 98-99 -11.692055207697612 1102 100-101 -11.446726155344647 1103 1 0.322153309837951 1103 2 0.25623507609610385 1103 3 0.3173577346590264 1103 4 0.15821395398260307 1103 5 0.08744776574710045 1103 6 0.1994572376960022 1103 7 0.2821314093951486 1103 8 0.25208947789951 1103 9 0.38140452580707773 1103 10-11 0.3555631703244799 1103 12-13 0.34416288014873686 1103 14-15 0.4575716167230581 1103 16-17 0.4114179579770223 1103 18-19 0.4627401971841607 1103 20-21 0.41243046440879993 1103 22-23 0.3887509662636077 1103 24-25 0.3718165748086264 1103 26-27 0.3004657221449136 1103 28-29 0.4103389404090052 1103 30-31 0.3961137976000728 1103 32-33 0.4644436442384503 1103 34-35 0.4780919115214104 1103 36-37 0.10546082312759353 1103 38-39 0.29236871980577206 1103 40-41 0.43730470862228543 1103 42-43 0.36772792810433685 1103 44-45 0.2665613838976597 1103 46-47 0.35855877471746567 1103 48-49 0.3643886073276832 1103 50-51 0.3962810373046466 1103 52-53 0.4883533603236643 1103 54-55 0.6106802403980609 1103 56-57 1.0621590845387345 1103 58-59 -6.632881677197641 1103 60-61 -8.487238492915882 1103 62-63 -2.4660746361756054 1103 64-65 -0.18667903970312594 1103 66-67 -1.7162656948904633 1103 68-69 -4.016349607524084 1103 70-71 -4.489983029263913 1103 72-73 -7.350081711909757 1103 74-75 -8.07748961011876 1103 76-77 -9.055956869441776 1103 78-79 -12.144003667944265 1103 80-81 -8.538474801979625 1103 82-83 -4.7535918811687345 1103 84-85 -3.75769158091391 1103 86-87 -3.4483622912246794 1103 88-89 -3.6004551258577457 1103 90-91 -8.798641080858411 1103 92-93 -14.17654453875331 1103 94-95 -12.197370002777168 1103 96-97 -12.127748025446664 1103 98-99 -11.779139252671778 1103 100-101 -11.451743856211507 1104 1 0.19066706949925205 1104 2 0.1411993846669688 1104 3 0.1536714253989473 1104 4 0.13721853233143833 1104 5 0.012691698204150725 1104 6 0.10203785109629138 1104 7 0.1605560635575145 1104 8 0.2061327014509331 1104 9 0.1750676971751659 1104 10-11 0.2307938108010248 1104 12-13 0.1188254115325762 1104 14-15 0.18405514943609802 1104 16-17 0.20115162607135062 1104 18-19 0.23798166816333577 1104 20-21 0.24250480104051775 1104 22-23 0.4050183812195556 1104 24-25 0.221215481912445 1104 26-27 0.2680293513478844 1104 28-29 0.14653054184650927 1104 30-31 0.1914232053684941 1104 32-33 0.25137418133291334 1104 34-35 0.24083005950545555 1104 36-37 0.351950263387522 1104 38-39 0.3535827206919109 1104 40-41 0.35803527525448686 1104 42-43 0.1683383746164182 1104 44-45 0.1972107217601078 1104 46-47 0.2108651794828873 1104 48-49 0.05274286322292454 1104 50-51 0.047459100613849614 1104 52-53 0.039448717976398484 1104 54-55 -0.016360570905042948 1104 56-57 0.5097040065098852 1104 58-59 -6.491736597691908 1104 60-61 -8.303720548742199 1104 62-63 -2.4834457778090417 1104 64-65 -0.479423587529638 1104 66-67 -2.0067043302471284 1104 68-69 -4.06610887498827 1104 70-71 -4.896041218600791 1104 72-73 -7.9047058442388085 1104 74-75 -8.747405427575563 1104 76-77 -9.199479736571199 1104 78-79 -12.260652119674685 1104 80-81 -8.727482826397484 1104 82-83 -5.144711853600182 1104 84-85 -4.221907600211896 1104 86-87 -3.9511378536723747 1104 88-89 -4.215563677031874 1104 90-91 -9.576681744472845 1104 92-93 -14.662759278082802 1104 94-95 -12.209223495614268 1104 96-97 -11.985188088460497 1104 98-99 -11.323040202802236 1104 100-101 -10.986941020588999 1105 1 0.21133659511342628 1105 2 0.21039056847070015 1105 3 0.20158938025416973 1105 4 0.20162330610890677 1105 5 0.19969478985668587 1105 6 0.12961539173655723 1105 7 0.11710072630143742 1105 8 0.17070635259285183 1105 9 0.1951248761253339 1105 10-11 0.28100258045628124 1105 12-13 0.2499550282341474 1105 14-15 0.2657239769351847 1105 16-17 0.387797016849575 1105 18-19 0.29797601675547014 1105 20-21 0.25199417971808913 1105 22-23 0.3511744265637944 1105 24-25 0.4405389751473834 1105 26-27 0.321503637442099 1105 28-29 0.3553560463177732 1105 30-31 0.3667850616897681 1105 32-33 0.4460472307661121 1105 34-35 0.552834314683146 1105 36-37 0.3436799266301023 1105 38-39 0.4884276394113627 1105 40-41 0.5249313225933392 1105 42-43 0.4406763952939272 1105 44-45 0.5329289492126534 1105 46-47 0.48361708267758985 1105 48-49 0.6867882739473004 1105 50-51 0.5546061369490829 1105 52-53 0.5136899333615261 1105 54-55 0.48074590826785624 1105 56-57 1.1932746989735676 1105 58-59 -6.2286223395223 1105 60-61 -8.395592494488685 1105 62-63 -2.1637416456325873 1105 64-65 0.25831252496662316 1105 66-67 -1.135960006587677 1105 68-69 -3.686732524468008 1105 70-71 -4.491755456824254 1105 72-73 -5.8026909511169045 1105 74-75 -6.180434582191433 1105 76-77 -8.243322693919692 1105 78-79 -11.559877211925606 1105 80-81 -7.418211525576368 1105 82-83 -3.3523615327996765 1105 84-85 -2.4597274865188687 1105 86-87 -2.248644253060693 1105 88-89 -2.3609727392306006 1105 90-91 -7.522630017469087 1105 92-93 -10.882035233004515 1105 94-95 -11.368541337441336 1105 96-97 -11.773180176489486 1105 98-99 -11.165561395581731 1105 100-101 -10.778576272076648 1106 1 0.09075368056784328 1106 2 0.08690597393369259 1106 3 0.027986240397837037 1106 4 0.005054945427140467 1106 5 0.03193287391461297 1106 6 0.01241308841525779 1106 7 0.10980900167400165 1106 8 0.11821405091367154 1106 9 0.025062760277990037 1106 10-11 0.13010171745721522 1106 12-13 0.10392870533760146 1106 14-15 0.1738056870277518 1106 16-17 0.10336004265269594 1106 18-19 0.14529907751825988 1106 20-21 0.18025119031320713 1106 22-23 0.1106009847867071 1106 24-25 0.1517217955081307 1106 26-27 0.26391164776714504 1106 28-29 0.3263469820454432 1106 30-31 0.2556997511308907 1106 32-33 0.13144084601156436 1106 34-35 0.2712075499050144 1106 36-37 0.17707767840828126 1106 38-39 0.20034236006647888 1106 40-41 0.25719302071602357 1106 42-43 0.22683000342802018 1106 44-45 0.28812139705474493 1106 46-47 0.3196117708701536 1106 48-49 0.20804731073774008 1106 50-51 0.24847550502856564 1106 52-53 0.22069944277254905 1106 54-55 -0.11827655087913769 1106 56-57 0.6748676033542438 1106 58-59 -6.598587420563327 1106 60-61 -8.685495446111062 1106 62-63 -2.5397741841852586 1106 64-65 -0.3881438046205794 1106 66-67 -1.6831144439283001 1106 68-69 -3.960690282434701 1106 70-71 -4.5792425810518775 1106 72-73 -6.060745790032339 1106 74-75 -6.580831203480191 1106 76-77 -8.330467414208382 1106 78-79 -11.728566794187373 1106 80-81 -7.953125666736906 1106 82-83 -3.998028487630311 1106 84-85 -3.0405261304744116 1106 86-87 -2.701846944521222 1106 88-89 -2.778141996432552 1106 90-91 -8.191755781363726 1106 92-93 -11.722831244127553 1106 94-95 -11.790048321911694 1106 96-97 -12.110458574098764 1106 98-99 -11.585226618714287 1106 100-101 -11.335825314960605 1107 1 0.056907529228084286 1107 2 0.12986698914852823 1107 3 -0.02362587189834997 1107 4 -3.0876545871194594E-5 1107 5 -0.06702336569696143 1107 6 0.0555689236971304 1107 7 0.020132849645925432 1107 8 -0.04365209996177555 1107 9 -0.0732644888314411 1107 10-11 0.08530918996756753 1107 12-13 0.01990070609624439 1107 14-15 0.05192740707580157 1107 16-17 0.13101444570160936 1107 18-19 0.18420416973467724 1107 20-21 0.07154912775926192 1107 22-23 0.06642889472308866 1107 24-25 0.1611121227393184 1107 26-27 0.21209847030072382 1107 28-29 0.22336292239982214 1107 30-31 0.2525994646842946 1107 32-33 0.20930200949330668 1107 34-35 0.17587041547604798 1107 36-37 0.1338440508627059 1107 38-39 0.26695616057697436 1107 40-41 0.12714125831396217 1107 42-43 0.2605545110186043 1107 44-45 0.27355204647351883 1107 46-47 0.10485618045063205 1107 48-49 0.2227054503948125 1107 50-51 0.20129286793599732 1107 52-53 0.11207150687825873 1107 54-55 0.03192272900616189 1107 56-57 0.729924093706714 1107 58-59 -6.1019895084099325 1107 60-61 -8.741507505133807 1107 62-63 -2.5373755215062275 1107 64-65 -0.3050476833862916 1107 66-67 -1.8355124074272986 1107 68-69 -4.136800563354377 1107 70-71 -4.5010299947523595 1107 72-73 -6.004973420785454 1107 74-75 -6.566075925315827 1107 76-77 -9.081416025019116 1107 78-79 -12.237713766038656 1107 80-81 -8.23766390448554 1107 82-83 -4.158232151306525 1107 84-85 -3.1574555734342873 1107 86-87 -3.0172649774739178 1107 88-89 -3.1582212848057587 1107 90-91 -8.642503978667172 1107 92-93 -11.872042847366373 1107 94-95 -11.982171726236917 1107 96-97 -12.440787741269453 1107 98-99 -11.706533064434632 1107 100-101 -11.105340549686202 1108 1 -0.09654089930082677 1108 2 0.014494321026365498 1108 3 0.008545080314871 1108 4 -0.006452662639503615 1108 5 -0.09210043454148575 1108 6 -0.06469501068412598 1108 7 -0.030313453370474974 1108 8 -0.0367895303969803 1108 9 -0.02586284238164893 1108 10-11 -0.019205967067726704 1108 12-13 -0.1304558669279956 1108 14-15 -0.06289064101796527 1108 16-17 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60-61 3.976773643602815 2311 62-63 1.3927912888149194 2311 64-65 1.3477882670576058 2311 66-67 -0.3156723920698461 2311 68-69 -3.189605840060697 2311 70-71 -0.7992385532655355 2311 72-73 -0.9902718354653075 2311 74-75 1.1443062213337143 2311 76-77 4.29040982535043 2311 78-79 5.212418636686419 2311 80-81 3.0879030160500527 2311 82-83 1.624444803928732 2311 84-85 1.2686157010607957 2311 86-87 1.2406458296275176 2311 88-89 1.4135700576090073 2311 90-91 2.9449305195862685 2311 92-93 4.849916576579805 2311 94-95 8.727705088599084 2311 96-97 9.406420376051418 2311 98-99 9.836971855264894 2311 100-101 9.004799140624849 2312 1 -0.27669392849944785 2312 2 -0.2913872173520069 2312 3 -0.29505778033096064 2312 4 -0.19343975033805094 2312 5 -0.31968656653571514 2312 6 -0.31529315896072063 2312 7 -0.3472367608015361 2312 8 -0.335469222827939 2312 9 -0.28889134245311254 2312 10-11 -0.3370041272912374 2312 12-13 -0.38870269821698145 2312 14-15 -0.5679527168745011 2312 16-17 -0.46894654497987176 2312 18-19 -0.39405536536633434 2312 20-21 -0.3631449271740337 2312 22-23 -0.3893325850234959 2312 24-25 -0.3671704092344825 2312 26-27 -0.49471287230533534 2312 28-29 -0.5384807904388254 2312 30-31 -0.4521201066891791 2312 32-33 -0.3208189788910758 2312 34-35 -0.37810148591523074 2312 36-37 -0.37894243028723196 2312 38-39 -0.5584756516257485 2312 40-41 -0.5417074597720628 2312 42-43 -0.35475928506147625 2312 44-45 -0.155791355299435 2312 46-47 -0.31592289099824455 2312 48-49 -0.2969702626569912 2312 50-51 -0.3369169923677049 2312 52-53 -0.2642562133038666 2312 54-55 -0.2996931466482735 2312 56-57 0.9081372470384252 2312 58-59 4.164173425856514 2312 60-61 3.9160970614398174 2312 62-63 1.4620749075574757 2312 64-65 1.3510118373837123 2312 66-67 -0.4423393485871223 2312 68-69 -0.9277130089049486 2312 70-71 0.09267346665208542 2312 72-73 6.9976181896759755 2312 74-75 5.3189155886053 2312 76-77 5.446360588687487 2312 78-79 5.4164116504724795 2312 80-81 3.1270215518420024 2312 82-83 1.813862993145655 2312 84-85 1.4193670451412395 2312 86-87 1.240448287479289 2312 88-89 1.335056915529858 2312 90-91 2.7774235100458675 2312 92-93 4.693126060654674 2312 94-95 8.64136060635736 2312 96-97 9.150800408796393 2312 98-99 9.769401510108993 2312 100-101 8.830722129596927 2313 1 -0.2762479284002026 2313 2 -0.21738157971710237 2313 3 -0.2484963382195957 2313 4 -0.2508746385596581 2313 5 -0.282583473340452 2313 6 -0.30095519376204294 2313 7 -0.3181649372360269 2313 8 -0.39269472701380437 2313 9 -0.4227141260988887 2313 10-11 -0.404428146907982 2313 12-13 -0.3726099616644589 2313 14-15 -0.3816480667334403 2313 16-17 -0.46887404727038984 2313 18-19 -0.5058347907826573 2313 20-21 -0.5221873522526792 2313 22-23 -0.5433007150788214 2313 24-25 -0.43581387905474145 2313 26-27 -0.6133700791707142 2313 28-29 -0.5243213463110763 2313 30-31 -0.5127228059277655 2313 32-33 -0.3273832289270828 2313 34-35 -0.4127572065170426 2313 36-37 -0.6444386644003686 2313 38-39 -0.8384277353599998 2313 40-41 -0.729832903805395 2313 42-43 -0.4797781356762556 2313 44-45 -0.3763541384368736 2313 46-47 -0.42339239415292695 2313 48-49 -0.5634472128635295 2313 50-51 -0.45791736393358917 2313 52-53 -0.5056196664702313 2313 54-55 -0.4361906600615626 2313 56-57 0.6140367330187786 2313 58-59 4.06258912699651 2313 60-61 3.89697963330514 2313 62-63 1.2428978715397925 2313 64-65 1.1225331878986395 2313 66-67 1.8832727971393233 2313 68-69 0.47867290160697706 2313 70-71 2.892035147921172 2313 72-73 8.194854441435467 2313 74-75 9.814537344211768 2313 76-77 8.70228845649233 2313 78-79 6.295720937320137 2313 80-81 3.4133433884183653 2313 82-83 2.003302896901616 2313 84-85 1.493086564937709 2313 86-87 1.392911999289396 2313 88-89 1.453385583109096 2313 90-91 2.8824248232347607 2313 92-93 4.6332207433894474 2313 94-95 8.572589509100293 2313 96-97 9.221999513649383 2313 98-99 9.834634259224556 2313 100-101 9.022052117626936 2314 1 -0.36760297507559514 2314 2 -0.40871391067430096 2314 3 -0.376096296393829 2314 4 -0.23187925021101563 2314 5 -0.5426589760609275 2314 6 -0.28685342013319115 2314 7 -0.37994159485246115 2314 8 -0.4753215293978741 2314 9 -0.552977592373253 2314 10-11 -0.4485114234520253 2314 12-13 -0.5396712688638985 2314 14-15 -0.6519222057896883 2314 16-17 -0.6498391768401177 2314 18-19 -0.7026846975760961 2314 20-21 -0.6344338286120959 2314 22-23 -0.6881067480851186 2314 24-25 -0.6103850748029345 2314 26-27 -0.7285694186120537 2314 28-29 -0.8393631179928676 2314 30-31 -0.7371048843078114 2314 32-33 -0.6610439570559876 2314 34-35 -0.8255535474835725 2314 36-37 -0.8302784789247255 2314 38-39 -0.6234503248797623 2314 40-41 -0.8109842662076474 2314 42-43 -0.8264320743293965 2314 44-45 -0.7962523864420419 2314 46-47 -0.734761971676626 2314 48-49 -0.7400173918129056 2314 50-51 -0.5767070488341375 2314 52-53 -0.7528625912451403 2314 54-55 -0.6797040605324582 2314 56-57 0.5993475958194452 2314 58-59 3.8206373420097997 2314 60-61 3.5820474739778057 2314 62-63 1.240769084234632 2314 64-65 1.1815623253143137 2314 66-67 1.8568522534764718 2314 68-69 -0.3273281945165678 2314 70-71 2.362264937241898 2314 72-73 8.061521468669092 2314 74-75 9.59816435933034 2314 76-77 8.643160523730856 2314 78-79 6.369320044556353 2314 80-81 3.321184818282685 2314 82-83 1.8670103905646513 2314 84-85 1.4208722150940183 2314 86-87 1.290931585286419 2314 88-89 1.4051702092552532 2314 90-91 2.699925300985978 2314 92-93 4.648974815091794 2314 94-95 8.507678082372905 2314 96-97 9.144884698313753 2314 98-99 9.3446088031975 2314 100-101 8.599825328223277 2315 1 -0.6580184883052489 2315 2 -0.5436706395422597 2315 3 -0.5735524504592924 2315 4 -0.36117532408003683 2315 5 -0.678589803561934 2315 6 -0.6646994589009338 2315 7 -0.640754013038908 2315 8 -0.7574292305250978 2315 9 -0.9598182019398109 2315 10-11 -0.8559770349345825 2315 12-13 -0.6239288846137896 2315 14-15 -0.7956017541635347 2315 16-17 -0.8784526919030142 2315 18-19 -0.8808378341583705 2315 20-21 -0.8929914882002663 2315 22-23 -0.8688112413545639 2315 24-25 -1.0517360599377739 2315 26-27 -1.1073508526446503 2315 28-29 -0.8417910837438356 2315 30-31 -0.9531110645842773 2315 32-33 -0.9224185320853522 2315 34-35 -1.064187402890859 2315 36-37 -1.0945596385849328 2315 38-39 -1.103889795877528 2315 40-41 -1.0888403448590154 2315 42-43 -1.1785223627729522 2315 44-45 -1.1935290488475019 2315 46-47 -1.047261780075793 2315 48-49 -0.9294726783098 2315 50-51 -0.9493369465346149 2315 52-53 -0.8456050582668055 2315 54-55 -0.8810133073419237 2315 56-57 0.44732574719330387 2315 58-59 3.8585007321489506 2315 60-61 3.4269745736930126 2315 62-63 1.084767303113292 2315 64-65 0.8374238546261736 2315 66-67 1.6374336623710377 2315 68-69 6.033084084491033 2315 70-71 4.803856189820847 2315 72-73 8.82791291989978 2315 74-75 9.73286702549425 2315 76-77 8.60855971256957 2315 78-79 6.006249495596975 2315 80-81 3.1374239085312787 2315 82-83 1.352098480963459 2315 84-85 1.0619448425392264 2315 86-87 1.1211096361081552 2315 88-89 0.9855574881862097 2315 90-91 2.2449323365685494 2315 92-93 4.211954775177507 2315 94-95 8.384596006707437 2315 96-97 9.084266410090919 2315 98-99 9.055580863197685 2315 100-101 8.199163079105409 2316 1 -0.5319044237073953 2316 2 -0.5053689414139129 2316 3 -0.6260384765266132 2316 4 -0.47435984072403414 2316 5 -0.6692413130937069 2316 6 -0.5665381399612386 2316 7 -0.5709982233734365 2316 8 -0.6931762529983487 2316 9 -0.8721327233339693 2316 10-11 -0.7480841217844443 2316 12-13 -0.7972880727049798 2316 14-15 -0.7883195295264684 2316 16-17 -1.0545028245387869 2316 18-19 -1.0071997096694432 2316 20-21 -1.0211414523029347 2316 22-23 -0.9910527859594467 2316 24-25 -2.4473615737251677 2316 26-27 -1.747092077318591 2316 28-29 -1.2244588522743456 2316 30-31 -0.8690559887443712 2316 32-33 -0.7057938360197369 2316 34-35 -0.8705003994616405 2316 36-37 -1.0122620138823564 2316 38-39 -1.3902401864101748 2316 40-41 -1.0809499803112672 2316 42-43 -1.13092639975914 2316 44-45 -1.0982763901671788 2316 46-47 -0.9889185776863982 2316 48-49 -1.1998072718456783 2316 50-51 -1.2133965556332384 2316 52-53 -1.1340089716186625 2316 54-55 -0.9611757997232289 2316 56-57 0.026755651951482662 2316 58-59 3.4102612199189046 2316 60-61 3.3002466754982898 2316 62-63 0.9565137681475484 2316 64-65 0.711308976644581 2316 66-67 5.731405241658603 2316 68-69 8.48019190880882 2316 70-71 5.365427170024171 2316 72-73 8.932919676407394 2316 74-75 9.883116083595876 2316 76-77 8.386555567566024 2316 78-79 5.831918042806851 2316 80-81 2.8580835302102336 2316 82-83 1.381683145534847 2316 84-85 0.9860661711961463 2316 86-87 0.8160207820682395 2316 88-89 1.0040595281526343 2316 90-91 2.414232197534158 2316 92-93 4.178023387891887 2316 94-95 8.114416769466175 2316 96-97 8.857692132972161 2316 98-99 9.180640782466638 2316 100-101 8.09054896570453 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 25.0 4 64.0 5 133.0 6 304.0 7 827.0 8 1871.0 9 3065.0 10 5262.0 11 8097.0 12 11217.0 13 15033.0 14 19613.0 15 26609.0 16 35385.0 17 45162.0 18 47898.0 19 48016.0 20 49321.0 21 49152.0 22 46632.0 23 45246.0 24 46916.0 25 49793.0 26 64804.0 27 93954.0 28 130896.0 29 172162.0 30 201452.0 31 211742.0 32 215845.0 33 223539.0 34 225789.0 35 215171.0 36 177346.0 37 105775.0 38 31191.0 39 1095.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.886490228949604 20.10383050158084 14.52339804205775 23.4862812274118 2 15.961683886350848 21.439020842582867 38.56125331174622 24.03804195932007 3 18.347754735264946 25.985024587776362 30.3342909524979 25.33292972446079 4 12.912578700643618 17.297807499824856 37.2435661176786 32.54604768185293 5 13.066124827690787 38.39468392260735 34.318775665405035 14.22041558429683 6 31.263649821352967 37.33426032817445 17.210235424199137 14.191854426273451 7 28.043167702809313 33.11911172978456 21.408021906725843 17.42969866068029 8 26.611282892444834 34.5875972480973 20.05272614501746 18.748393714440407 9 27.109344778115208 15.718388003691732 20.31896795928127 36.85329925891179 10-11 24.939384893740804 26.51090386565987 27.56683272362862 20.982878516970707 12-13 25.799685349724793 24.102348150020863 27.832176112774558 22.265790387479782 14-15 22.857910338537348 25.485021996827218 25.88751186747342 25.76955579716201 16-17 21.992470324488806 28.53956810975294 26.852339773561457 22.615621792196794 18-19 21.93198733541262 26.967419204809968 29.35551749300415 21.745075966773257 20-21 22.845650789976272 26.111651350437555 29.327317004821797 21.715380854764376 22-23 22.57621435816522 26.046486303727374 29.308089222999623 22.069210115107783 24-25 22.301771088117306 26.4393612873552 29.35762455795139 21.901243066576097 26-27 21.88029954241652 26.82167189688114 29.233446770986664 22.064581789715678 28-29 21.93931391133289 26.698882461087177 29.420624010152487 21.941179617427448 30-31 22.03123542324703 26.65806102768845 29.13612331195251 22.174580237112007 32-33 21.741816370837366 26.806825459316585 28.90812221434495 22.5432359555011 34-35 22.38092799471212 26.613040327126637 29.064148476412527 21.941883201748716 36-37 22.46382620965707 26.28670341252831 28.86790818041336 22.381562197401255 38-39 22.057129037817212 26.643714253886312 28.912213685947393 22.386943022349087 40-41 22.076700861308893 26.39847961746688 29.43836871180715 22.08645080941708 42-43 22.041553250003904 26.453691533845237 28.7888719068053 22.715883309345557 44-45 21.89968102832659 26.528521780528447 29.061804332301833 22.509992858843134 46-47 22.209626106751664 26.579864556105314 28.547289770028744 22.66321956711427 48-49 21.95363198590484 26.961322663189087 28.20024821168187 22.884797139224204 50-51 22.08604109249668 26.908202560792592 28.08641699075355 22.919339355957177 52-53 22.238981011451852 26.714364791639333 28.176403595144293 22.870250601764518 54-55 22.04635352781033 26.734411064342833 28.22432956618731 22.99490584165953 56-57 22.001434506868243 26.833000064060915 27.990653207773946 23.174912221296896 58-59 20.329663164241666 26.489042040606854 29.73822716487684 23.44306763027464 60-61 21.73214324245724 27.088487850948773 28.083750134382658 23.095618772211328 62-63 21.645968491459218 27.013888598038605 28.35842916479941 22.981713745702766 64-65 20.847092939799218 26.791643258734865 28.693457096239626 23.66780670522629 66-67 20.935386791525943 26.757117193005755 28.775547666812272 23.53194834865603 68-69 21.74848677589658 26.076922640749835 28.96611164022602 23.208478943127567 70-71 20.910970064525213 26.32410124621408 29.149863326675213 23.61506536258549 72-73 20.623588968960128 26.733200709823972 28.87029118369002 23.772919137525875 74-75 21.17466311612558 26.936057785984026 28.176079621193146 23.713199476697255 76-77 19.80027407701102 27.139840865960174 29.041327117841565 24.01855793918724 78-79 20.58932550063897 27.319255256816728 27.920088930265635 24.171330312278666 80-81 21.406793964905255 27.51849534220643 27.08166240196436 23.99304829092396 82-83 21.786959061165714 27.216983969728975 26.845982685083726 24.150074284021585 84-85 21.36867565295941 27.261896716332103 26.846138689445564 24.523288941262923 86-87 21.65841626569629 27.368231875802685 26.720542717861417 24.252809140639606 88-89 21.516439943832033 27.29619693512965 26.512579157541406 24.674783963496914 90-91 21.088129196143456 27.2809019033742 26.82521090781225 24.8057579926701 92-93 20.972650057391835 27.31849580941371 26.976967174614785 24.731886958579665 94-95 19.540541660762706 26.987938567954355 28.16042196215874 25.3110978091242 96-97 19.466142282002348 26.78725663539415 28.275985828198614 25.470615254404883 98-99 19.623474943376873 27.09199917415394 27.619071968946958 25.665453913522228 100-101 19.52463736871136 27.038779673611774 27.725626097297223 25.710956860379643 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 451.0 1 309.0 2 210.0 3 308.5 4 380.0 5 411.5 6 565.0 7 902.5 8 1301.5 9 1639.0 10 1843.0 11 2039.0 12 2292.0 13 2620.0 14 2914.5 15 3166.0 16 3452.0 17 3759.5 18 4157.5 19 4659.0 20 5260.0 21 5987.0 22 6887.5 23 8001.5 24 9483.0 25 11381.0 26 13807.5 27 16494.0 28 19466.5 29 23432.0 30 28249.5 31 34418.5 32 42518.0 33 49912.5 34 55643.0 35 61997.0 36 68977.5 37 74351.0 38 77484.5 39 79821.5 40 81990.0 41 84069.0 42 85858.0 43 88701.0 44 91890.5 45 93536.0 46 94406.0 47 95344.0 48 95908.5 49 95229.0 50 94132.0 51 92836.0 52 91659.5 53 90568.0 54 88365.0 55 83801.0 56 77211.0 57 69613.0 58 61488.5 59 52538.0 60 43444.0 61 35119.5 62 27519.5 63 20957.5 64 15671.5 65 11776.0 66 8840.5 67 6522.5 68 4587.0 69 3290.0 70 2465.0 71 1819.5 72 1334.5 73 1024.0 74 772.5 75 518.0 76 345.0 77 219.0 78 133.0 79 75.0 80 40.5 81 25.0 82 15.0 83 7.0 84 4.5 85 3.0 86 3.0 87 1.5 88 0.0 89 0.5 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022464141138772042 2 0.005711222323416621 3 0.003921705995412746 4 0.0 5 0.06724012415435836 6 0.0 7 0.0 8 2.6652370842610896E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.474863006813869E-4 16-17 0.0 18-19 0.0011232070569386023 20-21 0.0 22-23 0.0 24-25 0.0 26-27 7.614963097888827E-5 28-29 0.0021512270751535938 30-31 0.008490683854146043 32-33 2.2844889293666481E-4 34-35 9.328329794913813E-4 36-37 0.027242530482697282 38-39 0.026100286018013955 40-41 0.028670336063551436 42-43 0.029127233849424762 44-45 0.02981258052823476 46-47 0.031183273885854748 48-49 0.03146883500202558 50-51 0.026557183803887288 52-53 0.022407028915537874 54-55 0.01747634030965486 56-57 0.1486440796707899 58-59 0.733397095957673 60-61 0.3027899701798045 62-63 3.4267333940499726E-4 64-65 0.1349181086868453 66-67 1.3736822306359104 68-69 2.634872418908258 70-71 2.126954380279073 72-73 1.7962555703455059 74-75 1.513854663860299 76-77 1.0888635733671235 78-79 0.41408265585544973 80-81 1.3326185421305448E-4 82-83 7.614963097888827E-5 84-85 1.9037407744722068E-5 86-87 0.002703311899750534 88-89 0.0 90-91 0.21978687241281628 92-93 0.17207912860454277 94-95 1.249253733616407 96-97 1.2329957874024142 98-99 0.9685852312359694 100-101 0.392970170666553 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2626408.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.71745853658788 #Duplication Level Percentage of deduplicated Percentage of total 1 66.53867103976991 23.76592223942642 2 14.29619709363772 10.212476538457866 3 6.406630515149436 6.864856793522659 4 3.4672665693795763 4.953677997084491 5 2.161625989747157 3.86038933302024 6 1.459613224705083 3.1280204899715476 7 0.996577139317373 2.4916641856455737 8 0.7336952096595862 2.0964582583607534 9 0.5389667352737779 1.7325469817767165 >10 2.9919684592047755 19.249260666760833 >50 0.23395947589835592 5.836806550599037 >100 0.1612497810656942 10.682472874483523 >500 0.009515828976861686 2.2399700410256584 >1k 0.0037421799347206646 1.8633510904741242 >5k 2.1383885341260937E-4 0.5819988649770592 >10k+ 1.0691942670630469E-4 0.44012709441342573 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11525 0.4388122485158437 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8744 0.33292618663969953 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6496 0.24733400141942913 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.8074815489444136E-5 0.0 10-11 0.0 0.0 0.0 3.8074815489444136E-5 0.0 12-13 0.0 0.0 0.0 7.614963097888827E-5 3.8074815489444136E-5 14-15 0.0 0.0 0.0 7.614963097888827E-5 3.8074815489444136E-5 16-17 0.0 0.0 0.0 1.3326185421305448E-4 3.8074815489444136E-5 18-19 0.0 0.0 0.0 1.903740774472207E-4 3.8074815489444136E-5 20-21 0.0 0.0 0.0 3.9978556263916344E-4 3.8074815489444136E-5 22-23 0.0 0.0 0.0 9.518703872361035E-4 3.8074815489444136E-5 24-25 0.0 0.0 0.0 0.002265451521621926 3.8074815489444136E-5 26-27 0.0 0.0 0.0 0.004150154888349411 3.8074815489444136E-5 28-29 0.0 0.0 0.0 0.012945437266411008 3.8074815489444136E-5 30-31 0.0 0.0 0.0 0.04612763896546157 3.8074815489444136E-5 32-33 0.0 0.0 0.0 0.11477653129292936 3.8074815489444136E-5 34-35 0.0 0.0 0.0 0.21329511637186604 3.8074815489444136E-5 36-37 0.0 0.0 0.0 0.3720480595551034 3.8074815489444136E-5 38-39 0.0 0.0 0.0 0.6204100809927475 3.8074815489444136E-5 40-41 0.0 0.0 0.0 0.9014593315280794 3.8074815489444136E-5 42-43 0.0 0.0 0.0 1.1767783223322499 3.8074815489444136E-5 44-45 0.0 0.0 0.0 1.4947791813000872 3.8074815489444136E-5 46-47 0.0 0.0 0.0 1.8625818989281178 3.8074815489444136E-5 48-49 0.0 0.0 0.0 2.2040558816451976 3.8074815489444136E-5 50-51 0.0 0.0 0.0 2.551583760025099 3.8074815489444136E-5 52-53 0.0 0.0 0.0 2.8790842854575525 3.8074815489444136E-5 54-55 0.0 0.0 0.0 3.2344936506437687 3.8074815489444136E-5 56-57 0.0 0.0 0.0 3.538463940103746 3.8074815489444136E-5 58-59 0.0 3.8074815489444136E-5 0.0 3.739784527004182 3.8074815489444136E-5 60-61 0.0 3.8074815489444136E-5 1.9037407744722068E-5 3.891569778952851 5.7112223234166203E-5 62-63 1.9037407744722068E-5 7.614963097888827E-5 3.8074815489444136E-5 4.021956984596453 9.518703872361035E-5 64-65 3.8074815489444136E-5 7.614963097888827E-5 3.8074815489444136E-5 4.156532419943893 1.1422444646833242E-4 66-67 3.8074815489444136E-5 7.614963097888827E-5 3.8074815489444136E-5 4.267691843765325 1.1422444646833242E-4 68-69 3.8074815489444136E-5 7.614963097888827E-5 3.8074815489444136E-5 4.391530181144742 1.1422444646833242E-4 70-71 7.614963097888827E-5 7.614963097888827E-5 3.8074815489444136E-5 4.614210739534757 1.1422444646833242E-4 72-73 7.614963097888827E-5 7.614963097888827E-5 3.8074815489444136E-5 4.88865400958267 1.1422444646833242E-4 74-75 7.614963097888827E-5 7.614963097888827E-5 3.8074815489444136E-5 5.214802879065248 1.1422444646833242E-4 76-77 7.614963097888827E-5 7.614963097888827E-5 3.8074815489444136E-5 5.681733378820046 1.1422444646833242E-4 78-79 7.614963097888827E-5 7.614963097888827E-5 3.8074815489444136E-5 6.350041577698514 1.1422444646833242E-4 80-81 1.1422444646833242E-4 7.614963097888827E-5 3.8074815489444136E-5 7.0858564244397675 1.1422444646833242E-4 82-83 1.1422444646833242E-4 7.614963097888827E-5 3.8074815489444136E-5 7.768138080602862 1.1422444646833242E-4 84-85 1.1422444646833242E-4 7.614963097888827E-5 3.8074815489444136E-5 8.326885997910455 1.1422444646833242E-4 86-87 1.1422444646833242E-4 7.614963097888827E-5 3.8074815489444136E-5 8.819098175150243 1.1422444646833242E-4 88-89 1.1422444646833242E-4 7.614963097888827E-5 3.8074815489444136E-5 9.302781593720397 1.5229926195777654E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6395 0.0 47.49472 1 GTATCAA 11105 0.0 39.029854 1 ATCAACG 13930 0.0 30.902636 3 TCAACGC 14030 0.0 30.646435 4 CAACGCA 14320 0.0 29.99288 5 TATCAAC 14625 0.0 29.500265 2 AACGCAG 14680 0.0 29.305403 6 ACGCAGA 16735 0.0 25.62233 7 GTACATG 13100 0.0 25.38153 1 CGCAGAG 16890 0.0 25.276083 8 TACATGG 13520 0.0 24.308422 2 ACATGGG 13280 0.0 23.681196 3 GCAGAGT 17975 0.0 23.356714 9 GAGTACT 9750 0.0 22.01338 12-13 GGTAAGG 1050 0.0 21.104956 3 CATGGGG 5590 0.0 20.916195 4 GTACTTT 10265 0.0 20.816952 14-15 AGGTAAG 1025 0.0 20.700907 2 AGAGTAC 16150 0.0 20.26295 10-11 AGTACTT 10470 0.0 20.117027 12-13 >>END_MODULE