FastQCFastQC Report
Fri 27 May 2016
SRR939784_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939784_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718516
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107270.39459028381661176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74620.27448799271367175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53020.19503287823209428No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28450.10465268550929993No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65650.043.4932021
GTATCAA116850.041.746251
ATCAACG150550.032.278323
TCAACGC151600.032.0559434
TATCAAC154200.031.6345632
CAACGCA154850.031.383155
AACGCAG160100.030.3845376
GTACATG131050.027.7466071
ACGCAGA181600.026.6836227
TACATGG133900.026.418292
CGCAGAG183350.026.1975258
ACATGGG133750.025.1480143
GCAGAGT184950.024.5464329
GAGTACT102600.022.33042712-13
CATGGGA71900.022.3117124
AGAGTAC162450.021.4879810-11
GTACTTT108650.021.04815314-15
AGTACTT110350.020.89003612-13
CATGGGG59350.019.975024
CAGAGTA181150.019.91904310-11
Produced by FastQC (version 0.11.5)