FastQCFastQC Report
Fri 27 May 2016
SRR939784_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939784_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718516
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118140.4345753344839611No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89020.32745806903472335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66480.2445451856821884No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64850.045.7315861
GTATCAA113450.040.0270581
ATCAACG145450.030.915273
TCAACGC145600.030.8834234
CAACGCA148350.030.3109265
TATCAAC152400.029.4802632
AACGCAG153100.029.370526
GTACATG128050.027.3848951
TACATGG131900.026.2569562
ACATGGG129850.025.8624553
ACGCAGA173150.025.8051787
CGCAGAG174850.025.5271558
GCAGAGT186700.023.5512459
GAGTACT102700.022.2388512-13
TAAGGTG13100.022.0870115
CATGGGA66850.022.0667024
CATGGGG59150.021.3307114
AGAGTAC163300.021.20406510-11
GTACTTT107950.021.20123114-15
ATATAGG11100.020.5156172
Produced by FastQC (version 0.11.5)