##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939784_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2718516 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.394888240495916 31.0 31.0 34.0 30.0 34.0 2 31.624734597846768 31.0 31.0 34.0 30.0 34.0 3 31.664603776472163 31.0 31.0 34.0 30.0 34.0 4 35.4883671826835 37.0 35.0 37.0 33.0 37.0 5 35.223048163041895 37.0 35.0 37.0 32.0 37.0 6 35.24430498109998 37.0 35.0 37.0 32.0 37.0 7 35.14709495916154 37.0 35.0 37.0 32.0 37.0 8 35.10333027284003 37.0 35.0 37.0 32.0 37.0 9 36.68446461231054 39.0 35.0 39.0 32.0 39.0 10-11 36.54758496915228 38.0 35.0 39.0 32.0 39.0 12-13 36.46795825369429 38.0 35.0 39.0 32.0 39.0 14-15 37.477193071514016 39.0 36.0 41.0 32.0 41.0 16-17 37.406145853105144 39.0 36.0 41.0 32.0 41.0 18-19 37.40712175319182 39.0 36.0 41.0 32.0 41.0 20-21 37.32351363758757 39.0 36.0 41.0 31.0 41.0 22-23 37.18575796500738 39.0 36.0 40.0 31.0 41.0 24-25 37.02705538610036 39.0 36.0 40.0 31.0 41.0 26-27 36.718698363371786 39.0 35.5 40.0 30.0 41.0 28-29 36.487438367109114 38.0 35.0 40.0 30.0 41.0 30-31 36.202774785949394 38.0 35.0 40.0 30.0 41.0 32-33 36.13580056177709 38.0 35.0 40.0 30.0 41.0 34-35 35.97451127747639 38.0 35.0 40.0 29.5 41.0 36-37 35.98754136447974 38.0 35.0 40.0 29.5 41.0 38-39 35.86487940479291 38.0 35.0 40.0 29.0 41.0 40-41 35.75394921346793 38.0 35.0 40.0 28.5 41.0 42-43 35.49316318167706 38.0 34.0 40.0 27.5 41.0 44-45 35.21237818721685 38.0 34.0 40.0 26.5 41.0 46-47 34.87488210479541 38.0 33.5 40.0 25.5 41.0 48-49 34.52828418151668 38.0 33.0 40.0 24.5 41.0 50-51 34.269288832583655 38.0 33.0 40.0 24.0 41.0 52-53 33.99938845311192 37.0 33.0 40.0 23.5 41.0 54-55 33.71083046779934 37.0 33.0 40.0 23.0 41.0 56-57 33.321199139530535 36.5 32.0 40.0 22.0 41.0 58-59 32.94619619674852 36.0 31.5 39.0 21.0 41.0 60-61 32.61440543296416 36.0 31.0 39.0 20.0 40.0 62-63 32.2965183945947 35.0 31.0 39.0 19.0 40.0 64-65 31.843458526637328 35.0 30.5 38.5 17.5 40.0 66-67 30.93088710899623 34.5 29.5 38.0 10.5 40.0 68-69 29.046398292303593 34.0 26.0 37.0 7.5 39.0 70-71 28.398871295957058 33.0 25.0 36.0 6.5 39.0 72-73 28.951204443895126 33.0 26.0 36.0 7.0 39.0 74-75 29.047399941732916 33.0 26.5 35.5 7.0 38.5 76-77 28.573855000301634 32.5 27.0 35.0 5.5 37.0 78-79 29.09696908166073 33.5 28.5 35.0 2.0 37.0 80-81 28.913127787366342 34.0 28.5 35.0 2.0 37.0 82-83 28.48293848555609 33.0 27.5 35.0 2.0 36.0 84-85 28.137149091636758 33.0 27.0 35.0 2.0 36.0 86-87 27.770291769480114 33.0 26.5 35.0 2.0 35.5 88-89 27.315728691683255 33.0 26.0 35.0 2.0 35.0 90-91 26.83087905313046 32.0 25.0 35.0 2.0 35.0 92-93 26.348752223639664 32.0 24.5 34.0 2.0 35.0 94-95 25.55433074515655 32.0 22.0 34.0 2.0 35.0 96-97 25.031419347908933 31.0 19.5 34.0 2.0 35.0 98-99 24.358369970969456 31.0 10.0 34.0 2.0 35.0 100-101 22.870110015905738 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.17681188599422626 1101 2 0.2205279558414972 1101 3 0.35700994987873 1101 4 0.1660256055476168 1101 5 0.25693953653618706 1101 6 0.16683429496314517 1101 7 0.21945512842766135 1101 8 0.13388362408145582 1101 9 0.3034275947511773 1101 10-11 0.2337314904898804 1101 12-13 0.2847255736488563 1101 14-15 0.4080564383193348 1101 16-17 0.3369772142064704 1101 18-19 0.30151953246983965 1101 20-21 0.334893274871952 1101 22-23 0.26292795818989845 1101 24-25 0.22603120637563734 1101 26-27 0.08807661482160967 1101 28-29 0.17413305470348206 1101 30-31 0.07462511836045849 1101 32-33 0.10939936667322314 1101 34-35 0.06723224360087698 1101 36-37 0.18094401194941412 1101 38-39 0.19157004605039418 1101 40-41 0.09997444755904183 1101 42-43 -0.09126898437782671 1101 44-45 -0.1880636794173185 1101 46-47 -0.25677565202997243 1101 48-49 -0.11287929146562448 1101 50-51 -0.2130078065948382 1101 52-53 -0.3461671142930669 1101 54-55 -0.446118232367013 1101 56-57 -0.399964302229904 1101 58-59 -0.4277324055453775 1101 60-61 -0.4605934396411655 1101 62-63 -0.31398451715978837 1101 64-65 -0.4717165380932009 1101 66-67 -0.5844038635302518 1101 68-69 -9.01407423515565 1101 70-71 -6.756216094929652 1101 72-73 -2.0711186660467718 1101 74-75 -1.061297216747139 1101 76-77 -0.8123138401717078 1101 78-79 -0.9994929466365967 1101 80-81 -0.9797228372629654 1101 82-83 -0.9088583683767766 1101 84-85 -0.8567120056448552 1101 86-87 -1.0732284675484642 1101 88-89 -1.128560737243017 1101 90-91 -1.313450052363958 1101 92-93 -1.6358169558647937 1101 94-95 -1.3745352635939447 1101 96-97 -1.5588241324362109 1101 98-99 -1.358440575725897 1101 100-101 -1.1806152180888922 1102 1 0.2393700147742024 1102 2 0.14051259997233956 1102 3 0.22022615256538103 1102 4 0.1519052645380583 1102 5 0.24884714493934723 1102 6 0.20010593076888483 1102 7 0.2540843983153067 1102 8 0.1987678506181183 1102 9 0.2872195532843804 1102 10-11 0.42033815982673417 1102 12-13 0.3372535045360934 1102 14-15 0.4882771295822579 1102 16-17 0.4968590459523412 1102 18-19 0.36783258774420347 1102 20-21 0.3094435856268163 1102 22-23 0.35317120727329865 1102 24-25 0.19187704080540158 1102 26-27 0.3268038572098675 1102 28-29 0.22364944488968064 1102 30-31 0.1926233301778737 1102 32-33 0.07430479610072638 1102 34-35 -0.05414950606492397 1102 36-37 0.19560091591086604 1102 38-39 0.21401137232366807 1102 40-41 0.16371820737789022 1102 42-43 0.07454289567763084 1102 44-45 0.16667637016607983 1102 46-47 0.15105289338951877 1102 48-49 0.14414741146592291 1102 50-51 0.16135019069476897 1102 52-53 0.1388440780747544 1102 54-55 -0.12008531487898466 1102 56-57 -0.08171296738081679 1102 58-59 -0.28005900362537517 1102 60-61 -0.14314694921991844 1102 62-63 0.05370924115809572 1102 64-65 -0.09057185784171651 1102 66-67 0.22339835406495467 1102 68-69 -4.398137423015694 1102 70-71 0.24791677850287996 1102 72-73 0.5068236610083616 1102 74-75 0.11890355623886606 1102 76-77 -0.012920772922033308 1102 78-79 -0.13586478854849204 1102 80-81 -0.11130077046249909 1102 82-83 -0.10856093292592561 1102 84-85 -0.22419177432780657 1102 86-87 -0.34272282187310665 1102 88-89 -0.27359635350206446 1102 90-91 -0.4298244445725423 1102 92-93 -0.5717052555741446 1102 94-95 -0.41658115054413614 1102 96-97 -1.0827808298587875 1102 98-99 -0.6300400742126193 1102 100-101 -0.7378546395636896 1103 1 0.22913953076855975 1103 2 0.29771442732852194 1103 3 0.24659965742414158 1103 4 0.1791996442857453 1103 5 0.2578729533380084 1103 6 0.25426380171454355 1103 7 0.2367630988678613 1103 8 0.35962586121841866 1103 9 0.36212718343937667 1103 10-11 0.35872195085274683 1103 12-13 0.266725545897188 1103 14-15 0.32367494569567157 1103 16-17 0.46743235229506297 1103 18-19 0.3379906992835373 1103 20-21 0.33126754565675043 1103 22-23 0.2683111153801718 1103 24-25 0.315238554591339 1103 26-27 0.3704874986002764 1103 28-29 0.31360895371088304 1103 30-31 0.3117092502214618 1103 32-33 0.3659162063342052 1103 34-35 0.2403399665175172 1103 36-37 0.3754872327764005 1103 38-39 0.420490249854403 1103 40-41 0.3766644843353433 1103 42-43 0.3234155725106973 1103 44-45 0.4083784495414946 1103 46-47 0.36021618951225776 1103 48-49 0.29448936440884665 1103 50-51 0.20298681598509916 1103 52-53 0.18706537907188192 1103 54-55 0.022936785855691255 1103 56-57 0.3420548240087129 1103 58-59 0.2825688281765366 1103 60-61 0.08774036600427593 1103 62-63 0.08098247702997696 1103 64-65 0.00480501401510125 1103 66-67 0.11607808160576027 1103 68-69 -3.9339210032333263 1103 70-71 0.6843447610172895 1103 72-73 0.8918268483491403 1103 74-75 0.5498621039986809 1103 76-77 0.35484181628607914 1103 78-79 0.2994663389926764 1103 80-81 0.32401827486392776 1103 82-83 0.21861933555664592 1103 84-85 0.36975204179769605 1103 86-87 0.270282657936594 1103 88-89 0.02086573710456463 1103 90-91 0.07504613258172199 1103 92-93 0.10997986667554116 1103 94-95 7.906184155537233E-4 1103 96-97 -0.13239408079141768 1103 98-99 -0.05384916839059528 1103 100-101 -0.09819822360560337 1104 1 0.1716691212277226 1104 2 0.18536906775499062 1104 3 0.2573202918824222 1104 4 0.25315694147528944 1104 5 0.2711673560698671 1104 6 0.2101409900760629 1104 7 0.15824341200665515 1104 8 0.16213001095817958 1104 9 0.22602475742972672 1104 10-11 0.17215112329083837 1104 12-13 0.244350004520129 1104 14-15 0.2099661370918966 1104 16-17 0.1870551985362212 1104 18-19 0.17173797367662047 1104 20-21 0.21468387378521214 1104 22-23 0.238676240832973 1104 24-25 0.26305874045423394 1104 26-27 0.2772730332324045 1104 28-29 0.14768831057610043 1104 30-31 0.10052080813757414 1104 32-33 0.23175114326952695 1104 34-35 0.17694526764866225 1104 36-37 0.27729307970032835 1104 38-39 0.413322329744247 1104 40-41 0.32055390861899014 1104 42-43 0.3078721244513929 1104 44-45 0.30925725441645113 1104 46-47 0.3125181976041205 1104 48-49 0.25736959367486634 1104 50-51 0.18583085704144509 1104 52-53 0.18294778882343365 1104 54-55 0.19400522376659524 1104 56-57 0.23700494966293206 1104 58-59 0.15599479341064892 1104 60-61 0.22235319812548227 1104 62-63 0.24508402609399838 1104 64-65 0.2425495955963548 1104 66-67 0.5086713513321293 1104 68-69 -4.063393141875434 1104 70-71 0.5663001586744336 1104 72-73 1.0057313945220336 1104 74-75 0.689205174630505 1104 76-77 0.402792227025099 1104 78-79 0.06471437309543049 1104 80-81 0.17413455537497313 1104 82-83 0.19209650759809094 1104 84-85 0.04783669029124127 1104 86-87 0.05298051556565753 1104 88-89 0.0050906576475071574 1104 90-91 -0.032102239594419046 1104 92-93 -0.18977982543287908 1104 94-95 0.21970333495346495 1104 96-97 -0.2284078774495235 1104 98-99 0.2633489851688857 1104 100-101 0.2033109698626525 1105 1 0.09798749164817266 1105 2 0.2060033659295577 1105 3 0.11813678465060207 1105 4 0.09591991410070477 1105 5 0.309221938018446 1105 6 0.1178578818677849 1105 7 0.09395491680093926 1105 8 0.27783286229151116 1105 9 0.2949617307907175 1105 10-11 0.2010877042453174 1105 12-13 0.1614242524461389 1105 14-15 0.2297391927689958 1105 16-17 0.23718070449695006 1105 18-19 0.1970275465895952 1105 20-21 0.10283814750234654 1105 22-23 0.14182493109293404 1105 24-25 0.26443165024844006 1105 26-27 0.22777185113988452 1105 28-29 0.25895581434243553 1105 30-31 0.23750209419473123 1105 32-33 0.27506916591008945 1105 34-35 0.2911928807655997 1105 36-37 0.06581470232427478 1105 38-39 0.2566826322614446 1105 40-41 0.28881001823428676 1105 42-43 0.23248359072315594 1105 44-45 0.3016337964072804 1105 46-47 0.18781154069717587 1105 48-49 0.26405287905595287 1105 50-51 0.3250180831583762 1105 52-53 0.3246192943153261 1105 54-55 0.3815830959385167 1105 56-57 0.43874048922700126 1105 58-59 0.31669897584838935 1105 60-61 0.4021204497770512 1105 62-63 0.4620796260117217 1105 64-65 0.3959493106015124 1105 66-67 0.9287863649073422 1105 68-69 2.2297043765253513 1105 70-71 2.391654068299882 1105 72-73 1.3083356656487624 1105 74-75 0.796046628042621 1105 76-77 0.5199483288948521 1105 78-79 0.5010830305368685 1105 80-81 0.6200447178825605 1105 82-83 0.6640262986377863 1105 84-85 0.47522218838332364 1105 86-87 0.2716616681020163 1105 88-89 0.39300955158439166 1105 90-91 0.28525837530913023 1105 92-93 0.23601238686262604 1105 94-95 0.5698733649456322 1105 96-97 0.38728618962069916 1105 98-99 0.3235237522512655 1105 100-101 0.1409882260333042 1106 1 0.4688706661790718 1106 2 0.44047690767538583 1106 3 0.3467233067250177 1106 4 0.23989918294149248 1106 5 0.3372223901364322 1106 6 0.3387730947827592 1106 7 0.413112668032376 1106 8 0.4747633766745807 1106 9 0.5032431587353443 1106 10-11 0.5233058693410655 1106 12-13 0.46340218844739667 1106 14-15 0.6300298095038812 1106 16-17 0.6246271758123143 1106 18-19 0.5353481393878781 1106 20-21 0.5689954332553313 1106 22-23 0.5716163128477874 1106 24-25 0.5093598858713833 1106 26-27 0.6843872296711169 1106 28-29 0.7412938868982835 1106 30-31 0.5645091159183053 1106 32-33 0.7452257718828648 1106 34-35 0.7311445586523817 1106 36-37 0.7582996571098093 1106 38-39 0.8319022247887844 1106 40-41 0.7623708027177685 1106 42-43 0.7518604000635065 1106 44-45 0.8703623428479688 1106 46-47 0.7336528168242182 1106 48-49 0.8236736664359796 1106 50-51 0.7630668974709565 1106 52-53 0.8369885421695074 1106 54-55 0.8312791588246 1106 56-57 0.9131184662715981 1106 58-59 0.8387578308141741 1106 60-61 0.918246391889916 1106 62-63 0.8826064637029702 1106 64-65 0.9866490673876918 1106 66-67 1.490551038567407 1106 68-69 2.962333439908978 1106 70-71 3.1453706143750324 1106 72-73 2.086157221201823 1106 74-75 1.5843604203665542 1106 76-77 1.123154745870547 1106 78-79 1.0857826713542487 1106 80-81 1.0305703157230788 1106 82-83 0.9840698483945438 1106 84-85 1.0438925500189207 1106 86-87 1.0324094904805712 1106 88-89 1.2159350194543528 1106 90-91 1.179733183626606 1106 92-93 1.1403409355478793 1106 94-95 1.4973704884385093 1106 96-97 1.3880476022595865 1106 98-99 1.4901141306889762 1106 100-101 1.5933197733402906 1107 1 0.11319938662525075 1107 2 0.04751276604206822 1107 3 0.08963440819246671 1107 4 0.15476650216292143 1107 5 0.042488635696322774 1107 6 0.09547624665443522 1107 7 0.011424222752147273 1107 8 0.07305162586692404 1107 9 0.07949482628601601 1107 10-11 0.008725047379222417 1107 12-13 0.12286905441651186 1107 14-15 0.18742971628176974 1107 16-17 0.14218869436833614 1107 18-19 0.041927932691962155 1107 20-21 0.18437065224693328 1107 22-23 0.17797189090522636 1107 24-25 0.22377877459954476 1107 26-27 0.06347734973476804 1107 28-29 0.11335658680346228 1107 30-31 0.14527482304014683 1107 32-33 0.12001562031657187 1107 34-35 0.16912372833269984 1107 36-37 0.09376022255011662 1107 38-39 0.18067627368217387 1107 40-41 0.1031076592039355 1107 42-43 0.1710850492751419 1107 44-45 0.2943569906130534 1107 46-47 0.277786654842771 1107 48-49 0.3675402945279842 1107 50-51 0.2681133477980424 1107 52-53 0.31166186296607634 1107 54-55 0.3031972037319406 1107 56-57 0.17852261626275379 1107 58-59 0.22419388526873973 1107 60-61 0.34432125888768184 1107 62-63 0.404178943893271 1107 64-65 0.23297643772056276 1107 66-67 0.7529813228948434 1107 68-69 2.1604653813397796 1107 70-71 2.4287067604444346 1107 72-73 1.458270952175674 1107 74-75 0.8246958592337457 1107 76-77 0.47628371383750334 1107 78-79 0.6730196805318052 1107 80-81 0.7115268870781755 1107 82-83 0.9226212089551638 1107 84-85 0.8814007873174106 1107 86-87 0.872646081765911 1107 88-89 0.9030603228280292 1107 90-91 0.7780656329571762 1107 92-93 0.6229992122246699 1107 94-95 1.0512187132002246 1107 96-97 0.7113250023270972 1107 98-99 0.9960498074332769 1107 100-101 1.0562925139095896 1108 1 -0.1721099277163205 1108 2 -0.25007369767342524 1108 3 -0.15546869658805917 1108 4 -0.06427646204132742 1108 5 -0.06753789691085643 1108 6 -0.07762341354199975 1108 7 -0.07479976831055524 1108 8 -0.1400959792161629 1108 9 -0.3495351043568178 1108 10-11 -0.18619302737756271 1108 12-13 -0.19830699175953725 1108 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-0.6466391731062835 2316 62-63 -0.5614749421659653 2316 64-65 -0.33002464698044065 2316 66-67 0.049700102370412225 2316 68-69 1.371476275510691 2316 70-71 1.6302713237343802 2316 72-73 0.735366075737268 2316 74-75 0.302328617203937 2316 76-77 -0.04058220768673593 2316 78-79 -0.26458643152252037 2316 80-81 -0.4371372800600959 2316 82-83 -0.3706819413938298 2316 84-85 -0.29362515118476296 2316 86-87 -0.25788943040160106 2316 88-89 -0.4080012693243944 2316 90-91 -0.4462387435755524 2316 92-93 -0.39189656215751967 2316 94-95 -0.01901213946907987 2316 96-97 -0.011566904860227822 2316 98-99 -0.1942963536684026 2316 100-101 -0.5928444413818674 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 31.0 4 45.0 5 141.0 6 304.0 7 886.0 8 1924.0 9 3277.0 10 5316.0 11 8077.0 12 11194.0 13 14406.0 14 17266.0 15 21670.0 16 26595.0 17 31099.0 18 27795.0 19 23043.0 20 22736.0 21 23199.0 22 25249.0 23 28751.0 24 33815.0 25 40905.0 26 50002.0 27 61532.0 28 75175.0 29 90782.0 30 107494.0 31 126827.0 32 152623.0 33 185232.0 34 230739.0 35 290740.0 36 363644.0 37 389280.0 38 211536.0 39 15177.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.92530746594198 20.057346546660572 14.559532456026098 23.45781353137135 2 15.913537053314696 21.458371429801378 38.55547759449365 24.072613922390275 3 18.39056190256883 26.022304832713754 30.292247897196777 25.294885367520635 4 12.953979303414068 17.316800783957127 37.18672246181372 32.54249745081508 5 13.09526962504543 38.36714589871827 34.229005825237 14.308578650999296 6 31.276009144698484 37.36716552971016 17.18412442086948 14.17270090472188 7 28.008957828462293 33.146724168627294 21.431876803373605 17.412441199536808 8 26.604147262697737 34.579969365639194 20.04659895325244 18.769284418410635 9 27.108724024430973 15.701544519142061 20.311522904408143 36.87820855201882 10-11 24.925326906297407 26.561256214787775 27.540025513920096 20.973391364994725 12-13 25.811012331728044 24.087222587617656 27.838055762776452 22.26370931787784 14-15 22.88700894164316 25.490948002513136 25.89070654724857 25.73133650859513 16-17 21.948867521768385 28.581883364655674 26.83886780501209 22.630381308563855 18-19 21.930480072672015 26.955023798834326 29.374358910221403 21.740137218272253 20-21 22.78732220078896 26.158260609832716 29.340732223021675 21.713684966356645 22-23 22.525009968674087 26.065728507759378 29.3239399731324 22.085321550434134 24-25 22.259231840942427 26.480602903242122 29.343951885452064 21.916213370363387 26-27 21.890733018295155 26.79835416983553 29.22398750931808 22.086925302551236 28-29 21.890631813808607 26.73129019543578 29.412846611632936 21.96523137912268 30-31 22.073972711078984 26.675053637066732 29.103927621916846 22.147046029937435 32-33 21.711608347259777 26.841114179445707 28.924250556107545 22.523026917186968 34-35 22.35155086774469 26.628611157860465 29.061717427271027 21.958120547123812 36-37 22.469171624454436 26.29861390601014 28.87093157313917 22.361282896396258 38-39 22.08104884926805 26.649384915206998 28.88085532703453 22.38871090849042 40-41 22.102735195092148 26.415462217426295 29.411802611416192 22.069999976065365 42-43 22.04310716549011 26.494395361485466 28.767531871191814 22.694965601832607 44-45 21.89990213124134 26.547029657400433 29.044579256388904 22.508488954969323 46-47 22.190472613845728 26.595334979845685 28.57179412410108 22.642398282207505 48-49 21.96299739509025 26.9364788615716 28.198562237092812 22.901961506245343 50-51 22.12068785235018 26.910257108858165 28.065317696889007 22.903737341902648 52-53 22.25804539819126 26.700352354280227 28.15828628895701 22.8833159585715 54-55 22.055048133991193 26.727551119936543 28.228418838416648 22.988981907655614 56-57 21.920153899622665 26.93445779949371 27.890074957255727 23.255313343627897 58-59 21.795922902269922 26.577235232472862 28.103870503832685 23.522971361424535 60-61 21.691019308718023 27.16241749299366 27.962545074889984 23.184018123398328 62-63 21.724249111952723 27.018598182908033 28.264062304289794 22.99309040084945 64-65 21.509813280507803 26.749822734659308 28.03693004939518 23.703433935437708 66-67 21.236828080837817 27.085442955778383 27.985986549873175 23.69174241351062 68-69 21.380693386761703 26.74635856700857 28.310091401519514 23.562856644710205 70-71 21.3849767425542 26.6425628800455 28.125924434998257 23.846535942402046 72-73 21.286090080368883 27.110420241401474 27.596344630969437 24.007145047260206 74-75 21.55445970071928 27.41285329298573 27.106180336723924 23.926506669571065 76-77 21.54410352382221 27.077659056972987 27.455923705345086 23.92231371385972 78-79 21.534714382043056 27.22163547332978 27.21891325276296 24.0247368918642 80-81 21.73288332612954 27.465012552993866 26.830281683664552 23.97182243721204 82-83 21.776713307343645 27.228496892052057 26.85112160540648 24.14366819519782 84-85 21.359297585034724 27.23794715260987 26.857830419417716 24.544924842937686 86-87 21.700465203879148 27.336636520592073 26.712594199969065 24.25030407555972 88-89 21.53564042034094 27.269742680496563 26.517454890229292 24.67716200893321 90-91 21.372654289618225 27.16724796160529 26.700244013287477 24.759853735489006 92-93 21.55681276638557 27.20081634710055 26.620007750736036 24.62236313577785 94-95 21.089839683224536 26.698184195684536 27.14168015279203 25.070295968298893 96-97 21.46505660438446 26.42693802481686 26.965967833706124 25.14203753709256 98-99 21.226176208447196 26.767138735155687 26.677164572144434 25.329520484252683 100-101 21.041696672311865 26.63432586949232 26.928560536538644 25.395416921657173 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 716.0 1 479.5 2 359.0 3 538.5 4 529.5 5 538.5 6 895.5 7 1797.0 8 2781.5 9 3210.5 10 2982.0 11 2616.5 12 2633.0 13 2806.5 14 3043.0 15 3217.0 16 3441.5 17 3832.0 18 4189.5 19 4569.5 20 5146.5 21 6003.0 22 7007.0 23 8209.5 24 9560.5 25 11370.5 26 13841.5 27 16852.0 28 19547.0 29 22697.0 30 28041.0 31 34975.5 32 44840.5 33 52266.5 34 54802.5 35 60499.0 36 70323.0 37 77212.0 38 78661.0 39 79688.0 40 81475.5 41 82351.5 42 81708.0 43 85919.0 44 91022.5 45 92643.0 46 92720.0 47 92840.0 48 95118.0 49 94684.0 50 92903.5 51 91351.5 52 91176.0 53 93447.0 54 93500.0 55 88602.0 56 82616.0 57 76539.5 58 71239.0 59 63605.5 60 52257.0 61 42669.5 62 35537.0 63 27156.0 64 19656.0 65 15346.0 66 11505.5 67 8816.0 68 6506.5 69 4395.5 70 3272.5 71 2467.0 72 1835.0 73 1548.5 74 1225.5 75 874.0 76 637.5 77 406.5 78 223.0 79 131.5 80 90.0 81 59.0 82 33.5 83 17.0 84 13.5 85 9.5 86 3.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02405724299581095 2 0.02335833226657485 3 7.356955044590504E-5 4 0.0 5 0.0 6 3.678477522295252E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.310629770065727E-4 18-19 1.839238761147626E-5 20-21 0.0 22-23 0.0 24-25 1.2874671328033384E-4 26-27 3.4945536461804895E-4 28-29 5.517716283442878E-5 30-31 4.96594465509859E-4 32-33 0.002464579939937819 34-35 0.004358995863919874 36-37 0.1013420557392342 38-39 0.09376439204330597 40-41 0.10250077615875719 42-43 0.10369628135350317 44-45 0.09689109793725695 46-47 0.09093196435113864 48-49 0.09163087508037474 50-51 0.09078482525024682 52-53 0.07106818573074428 54-55 0.059628120636406046 56-57 0.033124690088268745 58-59 0.014033391747556386 60-61 0.00963761110841356 62-63 0.001894415923982055 64-65 0.004910767492264162 66-67 0.15694224348872693 68-69 0.4171393510282816 70-71 0.39657666167865113 72-73 0.1849538498210053 74-75 0.029427820178362016 76-77 0.030972780737726024 78-79 0.005407361957774021 80-81 0.0015081757841410536 82-83 7.356955044590504E-5 84-85 3.310629770065727E-4 86-87 0.001121935644300052 88-89 3.1267058939509645E-4 90-91 6.069487911787167E-4 92-93 0.0023726180018804376 94-95 0.07886655807801021 96-97 0.017822223595520496 98-99 0.01888898207698612 100-101 0.010207775124369324 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2718516.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.67386249457682 #Duplication Level Percentage of deduplicated Percentage of total 1 66.74922606694096 23.81202712331475 2 14.178947465543896 10.116356444072828 3 6.38420643683455 6.8324790769388395 4 3.4669887842934397 4.947235246444969 5 2.118648552512817 3.779018856833823 6 1.412518767734586 3.023400017470363 7 0.999275345270751 2.4953607882986684 8 0.7304399461458965 2.0846091359483845 9 0.5518793923455457 1.7718902600513051 >10 2.996202727651042 19.24839239831773 >50 0.237016595270763 5.8855017957778095 >100 0.16071849141801434 10.758739990416288 >500 0.0100099890345273 2.396070450821796 >1k 0.0036118517134886776 1.8415424540061505 >5k 2.063915264850673E-4 0.5724563732642113 >10k+ 1.0319576324253365E-4 0.43491958802208314 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11814 0.4345753344839611 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8902 0.32745806903472335 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6648 0.2445451856821884 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.678477522295252E-5 0.0 5 0.0 0.0 0.0 3.678477522295252E-5 0.0 6 0.0 0.0 0.0 3.678477522295252E-5 0.0 7 0.0 0.0 0.0 3.678477522295252E-5 0.0 8 0.0 0.0 0.0 3.678477522295252E-5 0.0 9 0.0 0.0 0.0 3.678477522295252E-5 0.0 10-11 0.0 0.0 0.0 3.678477522295252E-5 0.0 12-13 0.0 0.0 0.0 3.678477522295252E-5 0.0 14-15 0.0 0.0 0.0 3.678477522295252E-5 0.0 16-17 0.0 0.0 0.0 1.1035432566885757E-4 0.0 18-19 0.0 0.0 0.0 1.6553148850328634E-4 0.0 20-21 0.0 0.0 0.0 3.49455364618049E-4 0.0 22-23 0.0 0.0 0.0 9.012269929623367E-4 0.0 24-25 0.0 0.0 0.0 0.0020047702496509123 0.0 26-27 0.0 0.0 0.0 0.004156679600193635 0.0 28-29 0.0 0.0 0.0 0.012506823575803858 0.0 30-31 0.0 0.0 0.0 0.043406034763083974 0.0 32-33 0.0 0.0 0.0 0.11160500802643797 0.0 34-35 0.0 0.0 0.0 0.21083193919035237 0.0 36-37 0.0 0.0 0.0 0.3706433951464696 0.0 38-39 0.0 0.0 0.0 0.6157035676817793 0.0 40-41 0.0 0.0 0.0 0.8943850247708676 0.0 42-43 0.0 0.0 0.0 1.168744270771259 0.0 44-45 0.0 0.0 0.0 1.4856635017046065 0.0 46-47 0.0 0.0 0.0 1.855847087160789 0.0 48-49 0.0 0.0 0.0 2.2415170629858348 0.0 50-51 0.0 0.0 0.0 2.66044047561243 0.0 52-53 0.0 0.0 0.0 3.057789617570763 0.0 54-55 0.0 0.0 0.0 3.476915346461084 0.0 56-57 0.0 0.0 0.0 3.9389689080365904 0.0 58-59 0.0 0.0 0.0 4.411837193527646 1.839238761147626E-5 60-61 0.0 0.0 0.0 4.890204802914531 3.678477522295252E-5 62-63 0.0 0.0 0.0 5.3485247098049085 3.678477522295252E-5 64-65 0.0 0.0 0.0 5.824004714336793 3.678477522295252E-5 66-67 0.0 0.0 0.0 6.327275616549618 3.678477522295252E-5 68-69 0.0 0.0 0.0 6.875534298860114 3.678477522295252E-5 70-71 0.0 0.0 0.0 7.488295084524056 3.678477522295252E-5 72-73 0.0 0.0 0.0 8.107916230767081 3.678477522295252E-5 74-75 0.0 0.0 0.0 8.739657960446067 3.678477522295252E-5 76-77 0.0 0.0 0.0 9.407467162231159 3.678477522295252E-5 78-79 0.0 0.0 0.0 10.148496459097537 3.678477522295252E-5 80-81 0.0 0.0 0.0 10.911063241856954 3.678477522295252E-5 82-83 0.0 0.0 0.0 11.67833847584491 3.678477522295252E-5 84-85 0.0 0.0 0.0 12.427736309074511 3.678477522295252E-5 86-87 0.0 0.0 0.0 13.181345999067139 3.678477522295252E-5 88-89 0.0 0.0 0.0 13.958737046241406 3.678477522295252E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6485 0.0 45.731586 1 GTATCAA 11345 0.0 40.027058 1 ATCAACG 14545 0.0 30.91527 3 TCAACGC 14560 0.0 30.883423 4 CAACGCA 14835 0.0 30.310926 5 TATCAAC 15240 0.0 29.480263 2 AACGCAG 15310 0.0 29.37052 6 GTACATG 12805 0.0 27.384895 1 TACATGG 13190 0.0 26.256956 2 ACATGGG 12985 0.0 25.862455 3 ACGCAGA 17315 0.0 25.805178 7 CGCAGAG 17485 0.0 25.527155 8 GCAGAGT 18670 0.0 23.551245 9 GAGTACT 10270 0.0 22.23885 12-13 TAAGGTG 1310 0.0 22.087011 5 CATGGGA 6685 0.0 22.066702 4 CATGGGG 5915 0.0 21.330711 4 AGAGTAC 16330 0.0 21.204065 10-11 GTACTTT 10795 0.0 21.201231 14-15 ATATAGG 1110 0.0 20.515617 2 >>END_MODULE