FastQCFastQC Report
Fri 27 May 2016
SRR939783_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939783_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1788860
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31440.17575439106470042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24150.13500218015943113No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19800.053.414881
GTATCAA36150.041.1949461
ATCAACG44950.033.02923
TCAACGC45250.032.7053954
TATCAAC45700.032.5872882
CAACGCA45850.032.277415
AACGCAG48600.030.4510126
TGGTATC9000.025.8219092
ACGCAGA57950.025.0467517
TACATGG49000.024.778712
GTACATG49600.024.6695181
CGCAGAG58650.024.6662468
ACATGGG49550.024.2192273
GCAGAGT65550.021.418569
CATGGGG32850.020.792684
GTGGTAT11750.020.5851921
GAGTACA33900.020.2857421
GTAAGGT7900.019.2134914
CAGAGTA63100.019.16608610-11
TCTATAC6200.019.1263333
Produced by FastQC (version 0.11.5)