##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939783_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1788860 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.737253893541137 31.0 28.0 33.0 16.0 34.0 2 29.572515456771352 31.0 30.0 33.0 25.0 34.0 3 29.85873629015127 31.0 30.0 34.0 25.0 34.0 4 33.616283554889705 35.0 33.0 37.0 28.0 37.0 5 33.5695677694174 35.0 33.0 37.0 28.0 37.0 6 33.59099146942746 35.0 33.0 37.0 28.0 37.0 7 33.48290978612077 35.0 33.0 37.0 28.0 37.0 8 33.34978254307212 35.0 33.0 37.0 26.0 37.0 9 34.624243372874346 37.0 34.0 39.0 27.0 39.0 10-11 34.44297094238789 37.0 33.5 39.0 27.0 39.0 12-13 34.30270563375558 37.0 33.0 39.0 27.0 39.0 14-15 34.975791845085695 38.0 33.0 40.0 25.5 41.0 16-17 34.778442136332636 38.0 33.0 40.0 25.0 41.0 18-19 34.58413374998602 38.0 33.0 40.0 25.0 41.0 20-21 34.754887190724816 38.0 33.0 40.0 25.0 41.0 22-23 34.65294740784634 38.0 33.0 40.0 24.5 41.0 24-25 34.4362032243999 38.0 33.0 40.0 24.0 41.0 26-27 34.31643756358798 38.0 33.0 40.0 23.5 41.0 28-29 34.37368659369655 38.0 33.0 40.0 24.0 41.0 30-31 34.16696219938956 38.0 33.0 40.0 23.0 41.0 32-33 33.831399606453274 38.0 32.5 40.0 20.0 41.0 34-35 33.64820919468265 38.0 32.0 40.0 19.0 41.0 36-37 33.440311706897134 38.0 31.5 40.0 18.5 41.0 38-39 33.21391388929263 37.0 31.0 40.0 17.5 41.0 40-41 32.97337047057903 37.0 31.0 40.0 16.0 41.0 42-43 32.59819969142359 37.0 31.0 40.0 15.0 41.0 44-45 32.26804389387655 37.0 30.0 40.0 12.0 41.0 46-47 31.906003264648994 36.5 30.0 39.0 9.0 41.0 48-49 31.54985912815983 36.0 30.0 39.0 8.5 40.0 50-51 30.920514741231848 35.5 28.5 38.5 8.0 40.0 52-53 31.06934556085999 36.0 29.0 39.0 7.5 40.0 54-55 31.155392540500657 36.0 29.0 39.0 4.5 40.0 56-57 30.83027291123956 35.5 28.5 39.0 2.0 40.0 58-59 30.40299268808068 35.0 28.0 39.0 2.0 40.0 60-61 29.978739532439654 35.0 28.0 38.0 2.0 40.0 62-63 29.505338036514875 34.0 27.0 38.0 2.0 40.0 64-65 28.933756135192247 34.0 26.0 38.0 2.0 40.0 66-67 28.33045738626835 34.0 26.0 37.0 2.0 39.0 68-69 27.74631553056136 33.0 24.5 36.5 2.0 39.0 70-71 27.151628131882873 32.5 23.0 36.0 2.0 39.0 72-73 26.481154198763456 32.0 22.0 35.5 2.0 38.0 74-75 26.108893652940978 32.0 21.0 35.0 2.0 38.0 76-77 25.531366624554185 31.0 19.5 35.0 2.0 37.0 78-79 24.857941649989378 31.0 17.0 34.5 2.0 36.5 80-81 24.114616850955358 30.5 9.5 34.0 2.0 36.0 82-83 23.353139150073233 30.0 4.5 34.0 2.0 35.5 84-85 22.55736530527822 29.0 2.0 34.0 2.0 35.0 86-87 21.62236284561117 29.0 2.0 34.0 2.0 35.0 88-89 20.61699909439531 27.5 2.0 33.5 2.0 35.0 90-91 19.199061692921752 25.5 2.0 32.5 2.0 34.5 92-93 17.65936881589392 21.5 2.0 32.0 2.0 34.0 94-95 17.106367463077042 20.0 2.0 31.0 2.0 34.0 96-97 16.749430083964086 19.0 2.0 31.5 2.0 34.0 98-99 15.83954361995908 4.5 2.0 31.0 2.0 34.0 100-101 14.226350301309214 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.7062577329110802 1101 2 1.1284842176371939 1101 3 1.0189549611015636 1101 4 0.9823444116842168 1101 5 1.2550587925398347 1101 6 1.352150783630961 1101 7 1.0418257131307982 1101 8 1.3643497205917328 1101 9 1.259317605840721 1101 10-11 1.1716548671524691 1101 12-13 1.2821615037457477 1101 14-15 1.466224487634122 1101 16-17 1.2512867107364585 1101 18-19 1.3393040841896777 1101 20-21 1.3101800698617154 1101 22-23 1.6619927770138148 1101 24-25 1.5371317105200433 1101 26-27 1.5153170578581694 1101 28-29 1.6548661051331024 1101 30-31 1.639672637019686 1101 32-33 1.5558779145944257 1101 34-35 1.5631699724835855 1101 36-37 1.6304534240621962 1101 38-39 1.7208796592903681 1101 40-41 1.695664191560624 1101 42-43 1.8642332181128367 1101 44-45 1.984881298166023 1101 46-47 1.5565356542108582 1101 48-49 1.8064917459603613 1101 50-51 1.7284065422692692 1101 52-53 1.575922506131036 1101 54-55 1.8783108062721716 1101 56-57 1.8463759456624906 1101 58-59 1.6441241470303787 1101 60-61 1.6569997959051292 1101 62-63 1.0572663348135514 1101 64-65 1.5222544637321214 1101 66-67 1.7731140258450147 1101 68-69 1.5362364246992541 1101 70-71 1.69806414182856 1101 72-73 0.9753547552575448 1101 74-75 1.0268171343351362 1101 76-77 0.8066877226172942 1101 78-79 0.5775792608599346 1101 80-81 0.7650611932244367 1101 82-83 0.8606036491174365 1101 84-85 0.6749895324969266 1101 86-87 0.3304906498558111 1101 88-89 -3.7733031862979765 1101 90-91 -7.268136549822648 1101 92-93 -9.81640133676956 1101 94-95 -7.914771283943814 1101 96-97 -4.206209835059074 1101 98-99 -2.6792609672862895 1101 100-101 -1.75986257890019 1102 1 2.0221770986310794 1102 2 1.3541168555026282 1102 3 1.203077093437681 1102 4 1.2643687580009058 1102 5 1.2095057835157803 1102 6 1.2013355839600948 1102 7 1.1091474167327107 1102 8 1.0435715253303357 1102 9 1.5446422332771022 1102 10-11 1.5474855693114336 1102 12-13 1.2830551940256 1102 14-15 1.7324502275741054 1102 16-17 1.7053424343262051 1102 18-19 1.7087218546058267 1102 20-21 1.691510153699646 1102 22-23 1.7647689376848064 1102 24-25 1.8666467286665522 1102 26-27 1.772492664589393 1102 28-29 1.638448881305628 1102 30-31 1.8099388648345283 1102 32-33 2.14097501411743 1102 34-35 2.0354394893157917 1102 36-37 1.985121006847855 1102 38-39 1.9308909467616289 1102 40-41 2.065606319557233 1102 42-43 2.1734290094985553 1102 44-45 2.1051222121666413 1102 46-47 2.1223666883542727 1102 48-49 1.7747697426239135 1102 50-51 1.8540525780785373 1102 52-53 1.890285101917442 1102 54-55 1.8829455463105695 1102 56-57 1.9639034796227186 1102 58-59 1.7557509538261051 1102 60-61 1.8718553885981493 1102 62-63 1.8339086796671396 1102 64-65 1.6357907739383926 1102 66-67 1.8344831297122788 1102 68-69 1.7116439652517457 1102 70-71 1.9123153449757737 1102 72-73 1.7587867749205088 1102 74-75 1.619201292511498 1102 76-77 1.5649879266691649 1102 78-79 1.683354841743796 1102 80-81 1.590903929819678 1102 82-83 1.2495103887574466 1102 84-85 1.4658900166013886 1102 86-87 1.3830295602117104 1102 88-89 0.7331130060063877 1102 90-91 -5.455386449930899 1102 92-93 -10.225866509822383 1102 94-95 -8.293831720527077 1102 96-97 -4.7981517425231495 1102 98-99 -3.264919179810878 1102 100-101 -2.552871757084544 1103 1 2.3238399927874624 1103 2 1.466581340954015 1103 3 1.2369336123810548 1103 4 1.0580427979109785 1103 5 1.0557545806955986 1103 6 1.011013174944992 1103 7 0.9747489636392785 1103 8 0.9760974553212591 1103 9 1.0630632819082777 1103 10-11 1.1427425433882803 1103 12-13 1.1004650376171696 1103 14-15 1.135150321367476 1103 16-17 1.1273092372491504 1103 18-19 1.286920292193713 1103 20-21 0.9004481907883104 1103 22-23 0.9848931644975494 1103 24-25 0.9206191135872572 1103 26-27 0.9884196000087755 1103 28-29 1.0485451221220146 1103 30-31 1.041664738883867 1103 32-33 1.1596240855326485 1103 34-35 1.1503204082172047 1103 36-37 1.214020006435895 1103 38-39 1.272221748535351 1103 40-41 1.1611875420329838 1103 42-43 1.273915610119687 1103 44-45 0.978559077978268 1103 46-47 1.2262181451918401 1103 48-49 1.15438161744245 1103 50-51 1.008741866632242 1103 52-53 0.9497324658980517 1103 54-55 0.6870274581752049 1103 56-57 1.0308803079474096 1103 58-59 1.1456132128630259 1103 60-61 0.9543111635791881 1103 62-63 0.925317237210205 1103 64-65 0.6411941538441113 1103 66-67 0.8519335613594059 1103 68-69 0.950907360450195 1103 70-71 0.6679906040476595 1103 72-73 0.7043811467101371 1103 74-75 0.5550089264102773 1103 76-77 0.7386798739685396 1103 78-79 0.35893451366461093 1103 80-81 0.10419705006825097 1103 82-83 -0.3079270303894752 1103 84-85 -0.31776333404140544 1103 86-87 -0.22733498741388658 1103 88-89 -1.1827537313980123 1103 90-91 -6.711062621093921 1103 92-93 -9.964537653843093 1103 94-95 -8.662193255211623 1103 96-97 -5.596755330993711 1103 98-99 -4.108726309019229 1103 100-101 -3.2663862114488236 1104 1 1.9242924809173552 1104 2 1.2278012588157061 1104 3 1.0986741998265401 1104 4 0.6537635177986303 1104 5 0.8401396715199496 1104 6 0.8252642084550033 1104 7 0.8070309710097803 1104 8 0.7863527843861675 1104 9 0.8961449726603803 1104 10-11 0.9488782577708719 1104 12-13 0.7921830253149196 1104 14-15 0.8942741218732237 1104 16-17 0.7601804655386601 1104 18-19 0.7570612539590869 1104 20-21 0.6376370435521252 1104 22-23 0.7768930017497766 1104 24-25 0.6311969791502037 1104 26-27 0.6940501798223835 1104 28-29 0.4981360950454672 1104 30-31 0.6746444656895321 1104 32-33 0.8204249278756706 1104 34-35 0.9012497349962416 1104 36-37 0.7700390232667331 1104 38-39 0.7391208239553819 1104 40-41 0.6660657832720318 1104 42-43 0.709745518968603 1104 44-45 0.4453426271373431 1104 46-47 0.6372345666888428 1104 48-49 0.5267327432994939 1104 50-51 0.3994328172395285 1104 52-53 0.4271736516882001 1104 54-55 0.4993686712132721 1104 56-57 0.5518917853643046 1104 58-59 0.3568477410362654 1104 60-61 0.36335587113253354 1104 62-63 0.4160901781734516 1104 64-65 0.3315202781908084 1104 66-67 0.30714512730122934 1104 68-69 0.08712897360120309 1104 70-71 -0.05892652158005873 1104 72-73 0.19832729938974225 1104 74-75 0.1392745455730875 1104 76-77 0.20044228441102874 1104 78-79 0.18119532683633466 1104 80-81 -0.24322432241331882 1104 82-83 -0.46057211324905367 1104 84-85 -0.3584119332392568 1104 86-87 -0.2689355377972724 1104 88-89 -0.5987298913149601 1104 90-91 -2.8787201997769216 1104 92-93 -7.4777859391350106 1104 94-95 -5.460154099576666 1104 96-97 -2.773857089039554 1104 98-99 -1.9090900256359014 1104 100-101 -1.28374474109666 1105 1 2.1382707570865485 1105 2 1.3649763378734079 1105 3 1.1438946055860733 1105 4 0.9899265553974388 1105 5 0.7425547353875999 1105 6 0.8113831417567923 1105 7 0.8673171303184404 1105 8 1.037256086679335 1105 9 1.0316990366420455 1105 10-11 0.9744514636174983 1105 12-13 1.0038617917318717 1105 14-15 0.9925988443676133 1105 16-17 0.9391033263088175 1105 18-19 1.1282407031568482 1105 20-21 0.9925039034376155 1105 22-23 0.8655709272276582 1105 24-25 0.8216671078113009 1105 26-27 0.7898962322520617 1105 28-29 0.7031447830347801 1105 30-31 0.9938528514208258 1105 32-33 0.7635616734843111 1105 34-35 0.818643543935913 1105 36-37 0.753086943078344 1105 38-39 0.710735058370787 1105 40-41 0.6234153421862061 1105 42-43 0.5824996930181285 1105 44-45 0.5374318849764634 1105 46-47 0.5866144919901437 1105 48-49 0.43407814466574024 1105 50-51 0.32294765111334556 1105 52-53 0.31191056462743205 1105 54-55 0.22813538819912438 1105 56-57 0.27380969222322804 1105 58-59 0.1915380637219961 1105 60-61 0.20907183945973173 1105 62-63 0.36317016510932376 1105 64-65 0.4237548359005743 1105 66-67 0.3828004982959463 1105 68-69 0.20432639197824898 1105 70-71 -0.04492544318927827 1105 72-73 -0.040167651118292014 1105 74-75 0.24724006449936198 1105 76-77 0.1937443461261843 1105 78-79 -0.16744635511039263 1105 80-81 -0.07498378812126205 1105 82-83 -0.160049119437371 1105 84-85 -0.4647602946081797 1105 86-87 -0.04572921969598909 1105 88-89 -0.3524873288253012 1105 90-91 -3.3715529300515 1105 92-93 -7.826998962460998 1105 94-95 -5.645559306198226 1105 96-97 -3.142855754338086 1105 98-99 -2.1017722269589427 1105 100-101 -1.4030886543179193 1106 1 1.6626423333452216 1106 2 1.1717425169972238 1106 3 0.8018450608788754 1106 4 0.8920238176568063 1106 5 0.9168462605471674 1106 6 0.8967344516237716 1106 7 0.900881188940474 1106 8 -0.06298799785407283 1106 9 -0.4451532887362717 1106 10-11 -0.7649639441838829 1106 12-13 -0.18035175579468188 1106 14-15 0.45558817019193754 1106 16-17 0.6829792954628715 1106 18-19 0.8220567296582502 1106 20-21 0.7150356960047262 1106 22-23 0.8488363858567922 1106 24-25 0.7686766548007071 1106 26-27 0.9286634731575134 1106 28-29 0.5497996142455932 1106 30-31 0.6238642206946992 1106 32-33 0.9113097014301843 1106 34-35 0.7926940808453509 1106 36-37 0.8400507555284662 1106 38-39 0.6638408044463375 1106 40-41 0.594999985177779 1106 42-43 0.7402655889537897 1106 44-45 0.8495455888479313 1106 46-47 0.8018787162807222 1106 48-49 0.6085142210277432 1106 50-51 0.6896196058143715 1106 52-53 0.8016180319581636 1106 54-55 0.7985393035710935 1106 56-57 0.8006963898248962 1106 58-59 0.6278836979973619 1106 60-61 0.541846102318182 1106 62-63 0.794231591890572 1106 64-65 0.6087382794824663 1106 66-67 0.7339395478552682 1106 68-69 0.6140497303700059 1106 70-71 0.5612455885410128 1106 72-73 0.7728789385518091 1106 74-75 0.5311866358700819 1106 76-77 0.20735825143001207 1106 78-79 0.024142434813395397 1106 80-81 -0.04355651285750639 1106 82-83 -0.039615528451516724 1106 84-85 -0.03013292360316555 1106 86-87 0.10264432705127646 1106 88-89 -0.3423786597191807 1106 90-91 -3.8214978579568033 1106 92-93 -8.598350096987895 1106 94-95 -6.040071892067903 1106 96-97 -3.205588714864998 1106 98-99 -2.034480250229077 1106 100-101 -1.4555657853087922 1107 1 1.9648876002893587 1107 2 1.1860884583571156 1107 3 0.9181281327813835 1107 4 1.0604057368274766 1107 5 0.8690946789667962 1107 6 1.1027265750596342 1107 7 0.8486364531438966 1107 8 0.8869074368972818 1107 9 0.9554789184934194 1107 10-11 0.8953041649378974 1107 12-13 0.8317913396057932 1107 14-15 0.9605876850771793 1107 16-17 1.0549969904666128 1107 18-19 0.9705579728976303 1107 20-21 0.7881876389070754 1107 22-23 0.7688912427762631 1107 24-25 0.7692515335043169 1107 26-27 1.0479480598928532 1107 28-29 0.682825696423329 1107 30-31 0.9447869373090754 1107 32-33 1.0119724319393697 1107 34-35 1.008036702887125 1107 36-37 0.6812383448055215 1107 38-39 0.7947575226343204 1107 40-41 0.8093854793255986 1107 42-43 0.9377655482324769 1107 44-45 0.9386169092170178 1107 46-47 0.6622685290122625 1107 48-49 0.5634578591901906 1107 50-51 0.7816653524909825 1107 52-53 0.8965277262508735 1107 54-55 0.9068406441167021 1107 56-57 0.8276841741393319 1107 58-59 0.8987045051008558 1107 60-61 0.49812805526983794 1107 62-63 0.687584598929341 1107 64-65 0.8207426391415957 1107 66-67 0.994401207146062 1107 68-69 1.0472170269776697 1107 70-71 0.9113541551012396 1107 72-73 0.7074559007702241 1107 74-75 0.4271716560926393 1107 76-77 0.3672071080817858 1107 78-79 0.2185918366350208 1107 80-81 0.32769084114242375 1107 82-83 -0.040108079284109976 1107 84-85 0.23219483409723196 1107 86-87 0.40498165957160026 1107 88-89 0.0814549579551418 1107 90-91 -3.07213399567841 1107 92-93 -4.513864527558766 1107 94-95 -2.7784811901245394 1107 96-97 -1.2854239104016578 1107 98-99 -0.6592804502238359 1107 100-101 -0.14677253130765777 1108 1 1.797689966883965 1108 2 1.1214577059916948 1108 3 -0.41603686784169014 1108 4 0.024899593686789956 1108 5 0.40313705155735136 1108 6 0.6383437877732163 1108 7 0.4975726233566604 1108 8 0.6540848429983441 1108 9 0.8483771480624895 1108 10-11 0.8069654767869494 1108 12-13 0.6525562026109597 1108 14-15 0.6473407873496768 1108 16-17 0.6114302665494549 1108 18-19 0.7751030662412219 1108 20-21 0.641981703276862 1108 22-23 0.5340833931373723 1108 24-25 0.4836600564234743 1108 26-27 0.7343133216648638 1108 28-29 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1.964450092044352 2311 90-91 2.6673797242023163 2311 92-93 3.598035415340483 2311 94-95 3.035011537532313 2311 96-97 2.3183954987930555 2311 98-99 2.107079768630875 2311 100-101 1.7787535166039277 2312 1 1.1902275830648996 2312 2 0.4138652347128762 2312 3 -0.2673852962878982 2312 4 -0.707230973968521 2312 5 -0.16358839766868982 2312 6 -0.3610052574759024 2312 7 -0.0980778812108909 2312 8 -0.0273675001800342 2312 9 0.015410688273178152 2312 10-11 -0.1894027855985314 2312 12-13 -0.13925754981718796 2312 14-15 -0.03792854129761025 2312 16-17 -0.2912680392088589 2312 18-19 -0.5247537030154987 2312 20-21 0.1028928536453364 2312 22-23 0.2907823281174444 2312 24-25 0.4365424198963197 2312 26-27 0.37699530698371575 2312 28-29 0.34062810918278075 2312 30-31 0.28950476996477903 2312 32-33 0.3350895916394805 2312 34-35 0.09714323416153547 2312 36-37 0.21045316318094365 2312 38-39 0.10824267154577427 2312 40-41 0.25072217743691994 2312 42-43 0.24127486479532934 2312 44-45 0.1676220965830808 2312 46-47 0.1387117450457005 2312 48-49 0.20760922079033506 2312 50-51 -0.041163063860238935 2312 52-53 -0.11281053407878971 2312 54-55 0.012116802928883175 2312 56-57 0.30710328260668973 2312 58-59 0.037903834815718795 2312 60-61 -0.06787733905677129 2312 62-63 -0.04068454325255644 2312 64-65 0.2668010420737623 2312 66-67 0.31130955032671537 2312 68-69 0.4077940463451739 2312 70-71 0.4752048230497259 2312 72-73 0.22002374711202677 2312 74-75 0.3107046062151504 2312 76-77 0.47698799694396854 2312 78-79 0.6474658377165561 2312 80-81 0.7454223532229101 2312 82-83 0.6110882862215732 2312 84-85 0.8957437402105803 2312 86-87 1.2720462245872532 2312 88-89 1.517923076016121 2312 90-91 2.424694834593911 2312 92-93 3.4433230120808567 2312 94-95 3.030357360617515 2312 96-97 2.1987486145838204 2312 98-99 2.079325036233598 2312 100-101 1.9515570967642049 2313 1 -7.593373286594598 2313 2 -2.736539065650625 2313 3 -1.6866279392766934 2313 4 -0.9688649523183344 2313 5 -0.8102721075311123 2313 6 -0.39922690651921755 2313 7 -0.2378710802910149 2313 8 -0.3606884695878776 2313 9 -0.266298500786462 2313 10-11 -0.38799215680731436 2313 12-13 -0.5654591807140648 2313 14-15 -0.7376035602714808 2313 16-17 -0.8524922960893875 2313 18-19 -0.954029671538855 2313 20-21 -0.7366267222529999 2313 22-23 -1.3676566216716992 2313 24-25 -0.7369557911387687 2313 26-27 -0.8214143117698853 2313 28-29 -1.14609446144852 2313 30-31 -0.44862300835194446 2313 32-33 -0.7375603589999216 2313 34-35 -0.4541118944898628 2313 36-37 -0.4019862773609404 2313 38-39 -0.4986929200110026 2313 40-41 -0.45426980503293635 2313 42-43 -0.33010540580344383 2313 44-45 -0.38077981849387754 2313 46-47 -1.597614228898756 2313 48-49 -0.8355171023874597 2313 50-51 -1.0417993304873399 2313 52-53 -1.156654440470561 2313 54-55 -1.1594183971669842 2313 56-57 -0.8936816364476421 2313 58-59 -0.8114964196909291 2313 60-61 -0.775578447815569 2313 62-63 -0.9244260763412768 2313 64-65 -1.0590858793658562 2313 66-67 -0.890907681941993 2313 68-69 -0.9939255854761484 2313 70-71 -0.691499886464598 2313 72-73 -0.8421163913258916 2313 74-75 -0.7569950188725905 2313 76-77 -0.4484104543317038 2313 78-79 -0.4140822589046671 2313 80-81 -0.5791577963996097 2313 82-83 -0.19977075932142085 2313 84-85 0.15318920103310418 2313 86-87 0.6495834447583313 2313 88-89 0.8789302033436321 2313 90-91 1.7557189670743263 2313 92-93 2.5314174077987524 2313 94-95 2.2338571123879305 2313 96-97 1.523357296342084 2313 98-99 1.4339024467710857 2313 100-101 1.4910997836924924 2314 1 0.5498080118351574 2314 2 -0.14805795938063326 2314 3 -1.459612118023884 2314 4 -0.21488587902141632 2314 5 -0.26765986336049963 2314 6 -3.6301126531356296 2314 7 -2.7659302666859844 2314 8 -2.5854946020682235 2314 9 -3.3525862533488393 2314 10-11 -2.645193692897415 2314 12-13 -1.2711889274611607 2314 14-15 -1.2024044655310746 2314 16-17 -2.1432861505409804 2314 18-19 -2.964035641262555 2314 20-21 -3.157820429392011 2314 22-23 -4.308758317845431 2314 24-25 -2.921338578138432 2314 26-27 -1.9527868433188402 2314 28-29 -1.9786184665688253 2314 30-31 -2.191561062816021 2314 32-33 -2.2279969210330783 2314 34-35 -1.702989972535839 2314 36-37 -2.0007361993981334 2314 38-39 -3.6002138630491416 2314 40-41 -2.7511893088033688 2314 42-43 -1.740471934958567 2314 44-45 -2.390791728241158 2314 46-47 -1.856088415041274 2314 48-49 -2.6943524419637583 2314 50-51 -3.2531409145134305 2314 52-53 -2.1745858866584022 2314 54-55 -2.6270274485749177 2314 56-57 -1.4425413004205083 2314 58-59 -1.1323334284138546 2314 60-61 -1.5422290764185647 2314 62-63 -1.2301531500777578 2314 64-65 -1.7224602879622601 2314 66-67 -1.0758993213588006 2314 68-69 -0.9000732128822264 2314 70-71 -1.0850338737945293 2314 72-73 -2.0306527398151992 2314 74-75 -2.2498462004917776 2314 76-77 -1.3415835852290314 2314 78-79 -1.967995397939827 2314 80-81 -0.9445360719513616 2314 82-83 -0.5728190903232964 2314 84-85 -1.6079089905267132 2314 86-87 -0.5841980633542541 2314 88-89 0.5472897767311373 2314 90-91 1.7981634055787445 2314 92-93 2.771496825914042 2314 94-95 2.5030649211429363 2314 96-97 1.5376207779681081 2314 98-99 1.3748378222845297 2314 100-101 1.2061999176400224 2315 1 -3.293183304840369 2315 2 -2.2357772576262462 2315 3 -2.922929502393899 2315 4 -4.296727688933398 2315 5 -4.279680656083048 2315 6 -3.191457091673051 2315 7 -6.120499349325243 2315 8 -3.424705423735187 2315 9 -2.66979341266126 2315 10-11 -4.017483845889323 2315 12-13 -3.4596525106615843 2315 14-15 -2.5670745582713437 2315 16-17 -2.5380082250876157 2315 18-19 -2.1260948296375304 2315 20-21 -1.4651628595414579 2315 22-23 -0.9786000096169474 2315 24-25 -2.6547103071241978 2315 26-27 -4.437989749574374 2315 28-29 -3.687594188702441 2315 30-31 -3.575827705854593 2315 32-33 -4.374077610973416 2315 34-35 -4.0281420783896955 2315 36-37 -3.3339956949383307 2315 38-39 -2.1384470723349835 2315 40-41 -1.8045149630062127 2315 42-43 -3.0725500258085887 2315 44-45 -6.419160208364026 2315 46-47 -3.280640958014871 2315 48-49 -2.320925186957556 2315 50-51 -3.544785429842154 2315 52-53 -3.744259228261569 2315 54-55 -2.2025044407185774 2315 56-57 -1.7453500900714012 2315 58-59 -3.1043489048153 2315 60-61 -3.4049055487441606 2315 62-63 -5.360048595762681 2315 64-65 -4.894960140286674 2315 66-67 -4.226203533066521 2315 68-69 -2.4739257735768945 2315 70-71 -3.8279011429775878 2315 72-73 -2.8051373520191056 2315 74-75 -1.8580333349947047 2315 76-77 -3.7128252415584093 2315 78-79 -2.2596546808271825 2315 80-81 -2.048307706424751 2315 82-83 -1.7588312587916057 2315 84-85 -3.4530563707618143 2315 86-87 -2.755413138078101 2315 88-89 -2.0352018057604475 2315 90-91 -0.9443199070800041 2315 92-93 -0.30826275823150695 2315 94-95 -0.5560939187176608 2315 96-97 -0.7019090229652036 2315 98-99 -0.9024969287344327 2315 100-101 -1.2503243897614773 2316 1 -7.289641815535781 2316 2 -6.085746195294501 2316 3 -3.4153594971018 2316 4 -4.109551574460397 2316 5 -2.0335570561249767 2316 6 -2.9529910595471875 2316 7 -3.357703713902424 2316 8 -2.558252997677595 2316 9 -6.808183770299912 2316 10-11 -6.701748026546824 2316 12-13 -6.1526099897807995 2316 14-15 -5.233784686636746 2316 16-17 -4.828324702951555 2316 18-19 -4.146240285716928 2316 20-21 -2.8947082501726555 2316 22-23 -3.636780779604859 2316 24-25 -3.425860175844303 2316 26-27 -4.4226503264900785 2316 28-29 -4.525101762542587 2316 30-31 -6.973594924996149 2316 32-33 -4.910114070225518 2316 34-35 -5.795245854700003 2316 36-37 -4.3387579466633746 2316 38-39 -6.19265563480095 2316 40-41 -3.650131978516928 2316 42-43 -4.46621394927509 2316 44-45 -3.2539568587453367 2316 46-47 -4.522744031268285 2316 48-49 -5.660817082373367 2316 50-51 -5.044809397690724 2316 52-53 -6.54352146592548 2316 54-55 -5.56864176946031 2316 56-57 -4.8613429725790915 2316 58-59 -3.437181150327877 2316 60-61 -3.506678146910076 2316 62-63 -4.4080437331655595 2316 64-65 -3.430309521213122 2316 66-67 -4.306909670625355 2316 68-69 -3.3931138579130433 2316 70-71 -2.94585555421218 2316 72-73 -2.226419906748891 2316 74-75 -2.561462846510775 2316 76-77 -3.191958513022737 2316 78-79 -5.041159299271815 2316 80-81 -5.877824719123236 2316 82-83 -3.5519912822097908 2316 84-85 -2.4784456725368393 2316 86-87 -2.547295195810573 2316 88-89 -1.5003132466691547 2316 90-91 -1.2755990360052998 2316 92-93 -1.0439926207647083 2316 94-95 -1.6766256726017303 2316 96-97 -3.4159856919583476 2316 98-99 -4.52941435661997 2316 100-101 -4.072988834768367 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16143.0 3 5825.0 4 5599.0 5 6161.0 6 6776.0 7 7866.0 8 9409.0 9 11068.0 10 13146.0 11 15248.0 12 16848.0 13 18596.0 14 20381.0 15 21699.0 16 24180.0 17 25725.0 18 25137.0 19 25695.0 20 27570.0 21 30504.0 22 33255.0 23 37340.0 24 41570.0 25 47303.0 26 52844.0 27 59349.0 28 67798.0 29 76329.0 30 86836.0 31 97894.0 32 108792.0 33 120438.0 34 137048.0 35 157870.0 36 167156.0 37 127271.0 38 35640.0 39 551.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.37663274713501 19.022459211758523 12.663988406270867 23.936919634835597 2 16.191181919229344 19.396296491147556 39.31768395814048 25.094837631482626 3 19.876864522989955 23.099319956342875 29.09355497719195 27.93026054347522 4 13.095553965806971 15.534026777187778 36.17735996498075 35.1930592920245 5 14.062444905258031 36.83376289741674 34.41779374767378 14.685998449651452 6 33.950144229181376 35.18229191448914 16.83022716725827 14.037336689071218 7 29.600152312262534 31.670759125436426 20.3798287047018 18.349259857599236 8 27.040910880024278 34.86105955422376 19.458997017445732 18.639032548306236 9 26.697919810599675 15.944143031289915 19.55901037434898 37.79892678376143 10-11 25.573617661015668 25.850554391387305 27.27893984632979 21.29688810126724 12-13 26.225644292886003 23.052005309646322 27.541158996586574 23.181191400881097 14-15 22.8901581350871 24.686474633692022 25.168222710996783 27.255144520224096 16-17 22.99190407382795 27.78798873814737 26.18892995044807 23.031177237576607 18-19 23.139694597074982 26.515213872197986 27.863159421312357 22.481932109414675 20-21 23.429916794199617 25.916823318006983 28.126881146010245 22.526378741783155 22-23 23.084229106575776 25.86728346132495 27.998345230606176 23.050142201493102 24-25 23.00272614983094 26.11245879328299 28.09010105888691 22.79471399799916 26-27 22.7154156731661 26.304261942320462 27.900297840029364 23.080024544484075 28-29 22.758258590174947 26.739769411998072 27.831295494845797 22.670676502981184 30-31 22.879978215601117 26.329230735612867 27.75827161413823 23.03251943464778 32-33 22.639256588476226 26.322695959366744 27.808930687577217 23.229116764579814 34-35 23.164534516793463 26.53796173587225 27.62856136818389 22.668942379150394 36-37 23.29995257199574 26.06271085922007 27.63772400487335 22.999612563910844 38-39 22.830460333555553 26.376154755435543 27.721385314367986 23.071999596640914 40-41 23.19702377300807 26.1382356706329 27.878149712778182 22.78659084358085 42-43 22.892853381495833 26.129648842509077 27.702586119968537 23.274911656026546 44-45 22.90610820029257 26.38779385037574 27.809456483410667 22.896641465921018 46-47 23.229837501085473 26.549650267376684 27.32598105813377 22.894531173404072 48-49 22.94880428242794 26.613399737644684 27.29431295385691 23.143483026070474 50-51 22.962990779291786 26.802771340840565 27.346014901435694 22.888222978431955 52-53 23.140036129026473 26.551191288230154 27.322730403814454 22.98604217892892 54-55 22.73067888651559 26.7880973051803 27.42087354507704 23.06035026322707 56-57 22.727370784149638 26.90481787055764 27.345205312889686 23.022606032403033 58-59 22.69533271342293 26.475562801229245 27.356903936954048 23.472200548393776 60-61 22.409071851029665 26.674917775987144 27.554756025116312 23.36125434786688 62-63 22.577925000329067 26.601859742700963 27.657280388852744 23.162934868117226 64-65 22.370647899646592 26.66343850620375 27.48034607597865 23.485567518171006 66-67 22.379597448672786 26.80884959767554 27.344003168292875 23.467549785358802 68-69 22.767277101617324 26.410393218696477 27.573874672481136 23.248455007205067 70-71 22.536134012215626 26.47834200049185 27.25555727971291 23.729966707579614 72-73 22.622323837702893 26.61121711623111 27.170419808294177 23.59603923777182 74-75 22.596347963651677 26.87440125326675 27.04621827430369 23.48303250877788 76-77 22.768707624066867 26.601871224804235 27.10694947087708 23.522471680251815 78-79 22.807702881028156 26.81610084733485 27.015597384098513 23.360598887538487 80-81 22.91140453840695 26.766561620292673 26.795261590135283 23.526772251165095 82-83 22.830558158162983 26.869764403225 26.851182580283705 23.448494858328313 84-85 22.72685116304222 26.990951703283788 26.765451774593096 23.516745359080897 86-87 22.712722585706878 26.875744287375486 26.912282668086355 23.499250458831273 88-89 22.761717339081073 26.848239432377046 26.64729830720202 23.74274492133986 90-91 22.575583026140045 26.88761372515932 26.986503336892646 23.550299911807986 92-93 22.97978683108432 26.473061996593504 27.139935441363928 23.407215730958253 94-95 22.6668450543283 26.37988886673067 27.170207914185436 23.78305816475559 96-97 23.213576418370582 26.16399914672252 26.605943060827414 24.016481374079483 98-99 23.035721846442353 26.437452630286167 26.31735822593394 24.20946729733754 100-101 23.311148399176247 26.383337825289367 26.362892647672936 23.94262112786145 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2099.0 1 1206.0 2 326.0 3 332.5 4 285.0 5 234.5 6 277.0 7 410.0 8 622.0 9 762.5 10 762.5 11 743.5 12 770.5 13 856.0 14 945.0 15 1047.0 16 1202.5 17 1387.5 18 1561.5 19 1829.5 20 2121.0 21 2594.5 22 3223.5 23 4018.5 24 5043.5 25 6394.5 26 8403.0 27 10637.0 28 12707.0 29 14913.0 30 17512.0 31 20898.0 32 25470.0 33 28548.5 34 30284.5 35 33013.5 36 36632.0 37 40429.5 38 43541.0 39 46346.0 40 49100.0 41 51896.5 42 54049.0 43 56051.5 44 58773.0 45 62252.5 46 65283.5 47 67083.5 48 68321.5 49 68939.0 50 68912.0 51 68695.0 52 68904.5 53 69391.0 54 68473.0 55 64710.5 56 59955.5 57 54824.0 58 49562.0 59 43586.5 60 36193.0 61 29670.5 62 24222.0 63 18486.0 64 13457.5 65 10128.5 66 7490.0 67 5538.5 68 4237.5 69 3134.5 70 2310.0 71 1696.5 72 1180.5 73 839.0 74 621.5 75 458.5 76 334.5 77 218.0 78 149.5 79 107.5 80 73.0 81 57.0 82 43.0 83 29.5 84 23.0 85 18.0 86 8.5 87 5.5 88 5.0 89 2.0 90 1.5 91 2.5 92 3.0 93 4.0 94 3.5 95 1.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1328779222521606 2 0.11862303366389768 3 0.12354236776494526 4 0.1349462786355556 5 0.12102679919054593 6 0.13790905940095927 7 0.17077915543977729 8 0.15691557751864316 9 0.16843129143700458 10-11 0.1616951578100019 12-13 0.14964837941482287 14-15 0.15431615665843051 16-17 0.14830674284180986 18-19 0.1653008060999743 20-21 0.17033194324877296 22-23 0.2077859642453853 24-25 0.22967141084265957 26-27 0.24289212123922496 28-29 0.2374976241852353 30-31 0.2290005925561531 32-33 0.2300347707478506 34-35 0.21938553044956005 36-37 0.22637322093400267 38-39 0.21522086692083225 40-41 0.2188824167346802 42-43 0.21197857853605087 44-45 0.20468342967029282 46-47 0.2194134812114978 48-49 0.2592992184966962 50-51 0.26103216573683796 52-53 0.26268126069116643 54-55 0.26044519973614483 56-57 0.23375222208557406 58-59 0.23442304037208056 60-61 0.20378900528828414 62-63 0.19596279194570845 64-65 0.19730442851872143 66-67 0.2052424449090482 68-69 0.20445982357479064 70-71 0.21069284348691347 72-73 0.2434511364779804 74-75 0.24616236038594413 76-77 0.26167503326140673 78-79 0.25818118801918544 80-81 0.2731348456558926 82-83 0.2715975537493152 84-85 0.24487662533680668 86-87 0.24792325838802368 88-89 0.2519202173451249 90-91 0.45204767281956104 92-93 0.6029817872835214 94-95 0.34801493688717955 96-97 0.2878648971970976 98-99 0.31749270485113423 100-101 0.33784085954183113 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1788860.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.93321872092096 #Duplication Level Percentage of deduplicated Percentage of total 1 82.28981367528165 54.256322835561775 2 10.596008564916085 13.972579005587281 3 3.1890783426478317 6.307985996518549 4 1.3346056145786682 3.5197937556873797 5 0.6997392535112782 2.3068030624686533 6 0.4342388994251505 1.7178460999758265 7 0.27129481023919005 1.2521138042945885 8 0.20968039975754565 1.105992292696352 9 0.1396248667698929 0.8285325191656919 >10 0.7777815554936154 9.125409325000557 >50 0.03728107153584158 1.6609337796383472 >100 0.01809611509878545 2.163180779697284 >500 0.0018953211367718364 0.8484529884567407 >1k 8.615096076235617E-4 0.9340537552508963 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3144 0.17575439106470042 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2415 0.13500218015943113 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.590152387554085E-5 0.0 5 0.0 0.0 0.0 5.590152387554085E-5 0.0 6 5.590152387554085E-5 0.0 0.0 5.590152387554085E-5 5.590152387554085E-5 7 5.590152387554085E-5 0.0 0.0 5.590152387554085E-5 5.590152387554085E-5 8 5.590152387554085E-5 0.0 0.0 1.118030477510817E-4 5.590152387554085E-5 9 5.590152387554085E-5 0.0 0.0 1.6770457162662255E-4 5.590152387554085E-5 10-11 5.590152387554085E-5 0.0 0.0 1.6770457162662255E-4 5.590152387554085E-5 12-13 1.6770457162662255E-4 0.0 0.0 2.236060955021634E-4 1.6770457162662255E-4 14-15 2.236060955021634E-4 0.0 0.0 2.236060955021634E-4 1.9565533356439297E-4 16-17 2.236060955021634E-4 0.0 0.0 2.236060955021634E-4 2.236060955021634E-4 18-19 2.5155685743993384E-4 0.0 0.0 2.7950761937770426E-4 2.236060955021634E-4 20-21 2.7950761937770426E-4 0.0 0.0 3.074583813154747E-4 2.236060955021634E-4 22-23 3.354091432532451E-4 0.0 0.0 4.4721219100432683E-4 2.7950761937770426E-4 24-25 5.590152387554085E-4 0.0 0.0 6.987690484442607E-4 3.633599051910155E-4 26-27 5.590152387554085E-4 0.0 0.0 0.0014254888588262917 4.472121910043268E-4 28-29 7.826213342575719E-4 0.0 0.0 0.005590152387554085 4.751629529420972E-4 30-31 8.944243820086536E-4 2.7950761937770423E-5 0.0 0.022556264883780732 5.031137148798677E-4 32-33 8.944243820086536E-4 5.590152387554085E-5 0.0 0.05847299397381572 5.031137148798677E-4 34-35 9.503259058841944E-4 5.590152387554085E-5 0.0 0.11616336661337388 5.590152387554085E-4 36-37 0.001062128953635276 5.590152387554085E-5 0.0 0.21337611663293943 5.590152387554085E-4 38-39 0.0011739320013863578 5.590152387554085E-5 0.0 0.3615151549031227 5.86966000693179E-4 40-41 0.0011739320013863578 5.590152387554085E-5 0.0 0.5223438390930537 6.708182865064902E-4 42-43 0.001257784287199669 5.590152387554085E-5 0.0 0.6851849781425041 6.708182865064902E-4 44-45 0.0012857350491374394 5.590152387554085E-5 0.0 0.882573258947039 6.987690484442607E-4 46-47 0.0013975380968885211 5.590152387554085E-5 0.0 1.1151794997931643 7.26719810382031E-4 48-49 0.0013975380968885211 5.590152387554085E-5 0.0 1.3532081884552172 7.826213342575719E-4 50-51 0.001453439620764062 5.590152387554085E-5 0.0 1.6160851044799482 8.664736200708831E-4 52-53 0.001453439620764062 5.590152387554085E-5 0.0 1.8644835258209138 9.78276667821965E-4 54-55 0.001453439620764062 5.590152387554085E-5 0.0 2.125962903748756 0.0010062274297597353 56-57 0.0016211441923906845 5.590152387554085E-5 0.0 2.4317721901099025 0.0010341781916975057 58-59 0.0016211441923906845 5.590152387554085E-5 0.0 2.7664546135527655 0.001062128953635276 60-61 0.0016211441923906845 5.590152387554085E-5 0.0 3.119332983017117 0.001062128953635276 62-63 0.0016211441923906845 5.590152387554085E-5 0.0 3.4543508156032336 0.0011739320013863578 64-65 0.0016211441923906845 5.590152387554085E-5 0.0 3.79124134923918 0.0011739320013863578 66-67 0.0017329472401417664 5.590152387554085E-5 0.0 4.1826358686537795 0.0011739320013863578 68-69 0.001788848764017307 5.590152387554085E-5 0.0 4.606285567344566 0.0011739320013863578 70-71 0.001844750287892848 5.590152387554085E-5 0.0 5.058305289402188 0.0011739320013863578 72-73 0.001844750287892848 5.590152387554085E-5 0.0 5.504064040785751 0.001453439620764062 74-75 0.001844750287892848 5.590152387554085E-5 0.0 5.931319387766511 0.001453439620764062 76-77 0.001844750287892848 5.590152387554085E-5 0.0 6.38275214382344 0.001453439620764062 78-79 0.0018727010498306184 5.590152387554085E-5 0.0 6.87706136869291 0.0015372919065773732 80-81 0.002236060955021634 5.590152387554085E-5 0.0 7.3820757353845465 0.0016770457162662254 82-83 0.0022919624788971748 5.590152387554085E-5 0.0 7.864869246335655 0.001844750287892848 84-85 0.002319913240834945 5.590152387554085E-5 0.0 8.32178035173239 0.001844750287892848 86-87 0.002375814764710486 5.590152387554085E-5 0.0 8.785231935422559 0.0019006518117683888 88-89 0.0024037655266482562 5.590152387554085E-5 0.0 9.282084679628367 0.0019565533356439295 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1980 0.0 53.41488 1 GTATCAA 3615 0.0 41.194946 1 ATCAACG 4495 0.0 33.0292 3 TCAACGC 4525 0.0 32.705395 4 TATCAAC 4570 0.0 32.587288 2 CAACGCA 4585 0.0 32.27741 5 AACGCAG 4860 0.0 30.451012 6 TGGTATC 900 0.0 25.821909 2 ACGCAGA 5795 0.0 25.046751 7 TACATGG 4900 0.0 24.77871 2 GTACATG 4960 0.0 24.669518 1 CGCAGAG 5865 0.0 24.666246 8 ACATGGG 4955 0.0 24.219227 3 GCAGAGT 6555 0.0 21.41856 9 CATGGGG 3285 0.0 20.79268 4 GTGGTAT 1175 0.0 20.585192 1 GAGTACA 3390 0.0 20.285742 1 GTAAGGT 790 0.0 19.213491 4 CAGAGTA 6310 0.0 19.166086 10-11 TCTATAC 620 0.0 19.126333 3 >>END_MODULE