FastQCFastQC Report
Fri 27 May 2016
SRR939783_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939783_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1788860
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35880.20057466766544058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31710.17726373220934002No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20560.11493353308811198No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA18220.10185257650123541No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20750.049.0598261
GTATCAA37000.043.8174441
ATCAACG47200.033.243563
CAACGCA47750.032.959335
TCAACGC47750.032.8606534
TATCAAC49300.032.3053972
AACGCAG49750.031.6175336
CGCAGAG59450.026.1418728
ACGCAGA59550.026.0979717
GTACATG51400.022.372631
TACATGG48600.022.299592
GCAGAGT70650.021.6643569
TAAGGTG9300.021.285
ACATGGG49750.020.6475583
AGAGTAC63350.020.18356310-11
TGGTATC11250.020.1045342
GAGTACT44450.019.01810612-13
CAGAGTA67700.018.53886410-11
GTACTTT45600.018.48684314-15
AGTACTT48050.018.2793212-13
Produced by FastQC (version 0.11.5)