##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939783_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1788860 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.399537694397548 31.0 31.0 34.0 30.0 34.0 2 31.616806234137943 31.0 31.0 34.0 30.0 34.0 3 31.68218642040182 31.0 31.0 34.0 30.0 34.0 4 35.48997014858625 37.0 35.0 37.0 33.0 37.0 5 35.214890488914726 37.0 35.0 37.0 32.0 37.0 6 35.233389421195625 37.0 35.0 37.0 32.0 37.0 7 35.14972552351777 37.0 35.0 37.0 32.0 37.0 8 35.08567411647641 37.0 35.0 37.0 32.0 37.0 9 36.70507082723075 39.0 35.0 39.0 32.0 39.0 10-11 36.55752350659078 38.5 35.0 39.0 32.0 39.0 12-13 36.495376105452635 38.0 35.0 39.0 32.0 39.0 14-15 37.561254933309485 39.0 36.0 41.0 32.0 41.0 16-17 37.47047002001274 39.0 36.0 41.0 32.0 41.0 18-19 37.421246771686995 39.0 36.0 41.0 32.0 41.0 20-21 37.33253776147938 39.0 36.0 40.5 31.5 41.0 22-23 37.19167262949588 39.0 36.0 40.0 31.0 41.0 24-25 37.02759131513925 39.0 36.0 40.0 31.0 41.0 26-27 36.72504024909719 39.0 35.5 40.0 30.0 41.0 28-29 36.503020359335 38.0 35.0 40.0 30.0 41.0 30-31 36.25321461713047 38.0 35.0 40.0 30.0 41.0 32-33 36.22543211877956 38.0 35.0 40.0 30.0 41.0 34-35 36.33537140972463 38.0 35.0 40.0 30.0 41.0 36-37 36.31601299151414 38.0 35.0 40.0 30.0 41.0 38-39 36.18484006574019 38.0 35.0 40.0 30.0 41.0 40-41 36.01262535916729 38.0 35.0 40.0 29.0 41.0 42-43 35.82491558869894 38.0 35.0 40.0 28.5 41.0 44-45 35.60459650280067 38.0 34.0 40.0 28.0 41.0 46-47 35.34516871079906 38.0 34.0 40.0 27.0 41.0 48-49 35.045490703576576 38.0 34.0 40.0 26.5 41.0 50-51 34.77651381326655 38.0 33.0 40.0 26.0 41.0 52-53 34.48358284046823 38.0 33.0 40.0 25.5 41.0 54-55 34.156698959113626 37.0 33.0 40.0 24.0 41.0 56-57 32.84381645293651 36.5 31.5 39.5 16.0 41.0 58-59 29.354135315228696 34.5 24.0 38.5 8.0 40.0 60-61 29.36241433091466 34.0 24.0 38.0 9.0 40.0 62-63 31.217757398566683 35.0 29.5 38.0 12.5 40.0 64-65 30.693299084333038 35.0 29.0 38.0 4.5 40.0 66-67 25.093461198752276 28.0 18.0 36.0 2.0 39.0 68-69 17.267814977136275 13.0 7.0 29.0 2.0 35.5 70-71 16.14655450957593 12.5 7.5 25.5 2.0 33.0 72-73 18.19229788804043 17.0 7.0 29.5 2.0 34.0 74-75 18.946957280055454 17.5 7.5 30.5 2.0 35.0 76-77 20.03115280122536 23.0 8.5 30.5 2.0 34.5 78-79 23.17134963049093 27.5 12.0 33.5 2.0 35.5 80-81 25.862639893563497 31.5 22.0 34.5 2.0 36.0 82-83 26.86593556790358 32.5 25.5 35.0 2.0 36.0 84-85 26.86802991849558 33.0 26.0 35.0 2.0 36.0 86-87 26.58031735295104 33.0 25.0 35.0 2.0 35.5 88-89 26.129510414453897 33.0 24.5 35.0 2.0 35.0 90-91 24.45353493286227 31.5 11.0 34.0 2.0 35.0 92-93 22.273503516205853 30.5 2.0 34.0 2.0 35.0 94-95 17.71493828471764 20.0 2.0 33.0 2.0 35.0 96-97 16.353141106626566 13.5 2.0 32.5 2.0 34.5 98-99 15.212250539449705 2.0 2.0 31.5 2.0 34.0 100-101 14.571633610232215 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3698529484684343 1101 2 0.5204352853009233 1101 3 0.4239888422199911 1101 4 0.3214646978904767 1101 5 0.47978193821749215 1101 6 0.4273053289142794 1101 7 0.4449006388457022 1101 8 0.37134124764914844 1101 9 0.4541303851940697 1101 10-11 0.49963500531884364 1101 12-13 0.5277864569530237 1101 14-15 0.6572796157906353 1101 16-17 0.6397479852785608 1101 18-19 0.6600722972833921 1101 20-21 0.5855349508083023 1101 22-23 0.5859098846167399 1101 24-25 0.6737069426920854 1101 26-27 0.6702361627383411 1101 28-29 0.8408145406351011 1101 30-31 0.8433885747532415 1101 32-33 0.5050101999806387 1101 34-35 0.9161866848163385 1101 36-37 0.7818150736861185 1101 38-39 0.7635390158643744 1101 40-41 0.8677197817115783 1101 42-43 0.8251389670993916 1101 44-45 0.7161320831820177 1101 46-47 0.5946902045803952 1101 48-49 0.8060708467107105 1101 50-51 0.8063066838027666 1101 52-53 0.859044679684736 1101 54-55 0.9376918310785243 1101 56-57 1.3242010263131974 1101 58-59 -8.14815862262763 1101 60-61 -9.354485585163687 1101 62-63 -2.359677738075618 1101 64-65 0.38162855226257264 1101 66-67 -0.3376463201161606 1101 68-69 -3.1614988008324723 1101 70-71 -4.034814739945634 1101 72-73 -6.535486903610913 1101 74-75 -7.363181811303377 1101 76-77 -8.692172715441904 1101 78-79 -11.455352312219155 1101 80-81 -7.454397726031992 1101 82-83 -3.637842903741234 1101 84-85 -2.6916960473869587 1101 86-87 -2.290725194858023 1101 88-89 -2.5378838760763784 1101 90-91 -8.111549432030412 1101 92-93 -14.22210194725964 1101 94-95 -12.136960715769064 1101 96-97 -12.464306216010552 1101 98-99 -11.751086142145358 1101 100-101 -11.469969128321853 1102 1 0.5060056992560078 1102 2 0.49159828610835987 1102 3 0.49290051420038594 1102 4 0.43642972688576265 1102 5 0.44813789194360254 1102 6 0.4707721713746196 1102 7 0.4639660314825278 1102 8 0.6372222573087143 1102 9 0.594388479389913 1102 10-11 0.6907854262869861 1102 12-13 0.64793014693646 1102 14-15 0.8221350899177793 1102 16-17 0.8992821636703994 1102 18-19 0.8851337440479057 1102 20-21 0.8577891726426898 1102 22-23 0.8273055590965015 1102 24-25 0.7921032680985078 1102 26-27 0.890150368471474 1102 28-29 0.973755341879567 1102 30-31 0.9484044447955498 1102 32-33 0.7496557650833822 1102 34-35 0.8696812954045114 1102 36-37 0.9571034555346287 1102 38-39 0.8008644424347509 1102 40-41 0.9336839060492395 1102 42-43 1.0228419311276937 1102 44-45 0.976238934748217 1102 46-47 0.8465599884229533 1102 48-49 0.9305273703829755 1102 50-51 0.8712446204957089 1102 52-53 0.7800667649501989 1102 54-55 0.5557312732023689 1102 56-57 1.3540739948351472 1102 58-59 -8.38740084440984 1102 60-61 -9.620213052015632 1102 62-63 -2.5985879754089325 1102 64-65 0.40620192260426435 1102 66-67 -0.7974261077172535 1102 68-69 -3.6425383389946795 1102 70-71 -4.137558887830476 1102 72-73 -6.571211394459404 1102 74-75 -7.500495914832726 1102 76-77 -8.678936614030139 1102 78-79 -11.713799241540851 1102 80-81 -7.649397980948621 1102 82-83 -3.4909652767660724 1102 84-85 -2.587937272030164 1102 86-87 -2.256211854418641 1102 88-89 -2.412400571930011 1102 90-91 -8.161823141351942 1102 92-93 -14.167297539454685 1102 94-95 -12.24655767479151 1102 96-97 -12.04298534474108 1102 98-99 -11.541575202664937 1102 100-101 -11.229561700406942 1103 1 0.31231037721943267 1103 2 0.3781505664122058 1103 3 0.2611734240295327 1103 4 0.14855886682942554 1103 5 0.2303463382115538 1103 6 0.24257852226519816 1103 7 0.32639198108341105 1103 8 0.49793955618121544 1103 9 0.48620443764046684 1103 10-11 0.35441199185341077 1103 12-13 0.3534937508517544 1103 14-15 0.5028708130312296 1103 16-17 0.4780663157828826 1103 18-19 0.5007949569163017 1103 20-21 0.4107330227921935 1103 22-23 0.3313531155283371 1103 24-25 0.5397122971926862 1103 26-27 0.48431176907862294 1103 28-29 0.5725199144356523 1103 30-31 0.404137165609022 1103 32-33 0.38319654847845896 1103 34-35 0.6493615770582508 1103 36-37 0.3185963359032371 1103 38-39 0.18823380438139026 1103 40-41 0.3908376648528247 1103 42-43 0.42775460600216775 1103 44-45 0.43642524456429044 1103 46-47 0.2763119452384757 1103 48-49 0.23723458456781543 1103 50-51 0.4211983318705492 1103 52-53 0.33707291700491027 1103 54-55 0.1244728458603106 1103 56-57 0.7860215070305685 1103 58-59 -6.5624673130513855 1103 60-61 -8.408626910328355 1103 62-63 -2.3109097185975074 1103 64-65 -0.21427193914400178 1103 66-67 -1.6301037488671568 1103 68-69 -4.084222272053271 1103 70-71 -4.84742528168055 1103 72-73 -7.76015472167666 1103 74-75 -8.287202338538584 1103 76-77 -9.064472824808322 1103 78-79 -12.190236735175215 1103 80-81 -8.771169140322868 1103 82-83 -4.941449521151771 1103 84-85 -3.995880673734863 1103 86-87 -3.673114727353706 1103 88-89 -3.7546285673513147 1103 90-91 -9.19112710940443 1103 92-93 -14.490486392484879 1103 94-95 -12.382330953257496 1103 96-97 -12.229786017864338 1103 98-99 -11.937956481082276 1103 100-101 -11.581290037102345 1104 1 0.14691622553603878 1104 2 0.07890894638140722 1104 3 0.15047066187923974 1104 4 0.0876248672165687 1104 5 -0.01895686705654498 1104 6 0.06915054863527104 1104 7 0.14914831191018152 1104 8 0.08336524641055121 1104 9 0.10553048924384001 1104 10-11 0.2010484762055853 1104 12-13 0.14047442208769212 1104 14-15 0.15524982793868247 1104 16-17 0.2213280031074163 1104 18-19 0.29386399772776883 1104 20-21 0.19198521896244358 1104 22-23 0.10667007298335562 1104 24-25 0.18327913659393147 1104 26-27 0.07365980396089356 1104 28-29 0.13647695395501103 1104 30-31 0.07135396907012392 1104 32-33 0.23788189661691206 1104 34-35 0.16807054335096439 1104 36-37 0.17302584947233157 1104 38-39 0.2926183773719302 1104 40-41 0.2768094231822431 1104 42-43 0.14883303193528974 1104 44-45 0.1560563249505975 1104 46-47 -0.012304834303634493 1104 48-49 -0.07471788321860373 1104 50-51 -0.15095934606393513 1104 52-53 -0.056532205111402334 1104 54-55 -0.09790386345366642 1104 56-57 0.6784811067450178 1104 58-59 -6.689425125622666 1104 60-61 -8.703512931404195 1104 62-63 -2.701242254220162 1104 64-65 -0.743249920109907 1104 66-67 -2.0842396999737822 1104 68-69 -4.507594578546783 1104 70-71 -5.021116857387241 1104 72-73 -7.779445141380977 1104 74-75 -8.83569661339245 1104 76-77 -9.361727195880643 1104 78-79 -12.417079884541916 1104 80-81 -8.729905305220885 1104 82-83 -5.141445699044954 1104 84-85 -4.261224274973227 1104 86-87 -3.9737768148898134 1104 88-89 -4.115992122453147 1104 90-91 -9.82994479614209 1104 92-93 -15.07757494576813 1104 94-95 -12.607252199362954 1104 96-97 -12.36620613398241 1104 98-99 -11.70782447044449 1104 100-101 -11.298510354435845 1105 1 0.24896988276378806 1105 2 0.2063142164377183 1105 3 0.13432480309699812 1105 4 0.19339631523479284 1105 5 0.1885844962591392 1105 6 0.23953114857371816 1105 7 0.35664338500158976 1105 8 0.22007742322549717 1105 9 0.14875441477487072 1105 10-11 0.2691259660410239 1105 12-13 0.36920513556577816 1105 14-15 0.33795134257196935 1105 16-17 0.39712798819168427 1105 18-19 0.36125056310456927 1105 20-21 0.3301049614859295 1105 22-23 0.300327369308512 1105 24-25 0.4122537309758343 1105 26-27 0.38153437116873334 1105 28-29 0.38990350208441527 1105 30-31 0.3453603794089517 1105 32-33 0.47227973366331355 1105 34-35 0.5759825713514104 1105 36-37 0.33488090449341 1105 38-39 0.4384492997560798 1105 40-41 0.27925723309743944 1105 42-43 0.16842536936016472 1105 44-45 0.24276305837805268 1105 46-47 0.07987486091810325 1105 48-49 0.3265690707389126 1105 50-51 0.2800639336327393 1105 52-53 0.13688763950775495 1105 54-55 0.24873405184036557 1105 56-57 0.8991298899550415 1105 58-59 -6.74528885406021 1105 60-61 -9.023378381152295 1105 62-63 -2.72272777609529 1105 64-65 -0.11650085684403066 1105 66-67 -1.5998630792801656 1105 68-69 -4.143502248408332 1105 70-71 -4.8476395271673525 1105 72-73 -6.125649728742204 1105 74-75 -6.846434230971763 1105 76-77 -8.522158826505228 1105 78-79 -11.948768543351246 1105 80-81 -8.23607390621887 1105 82-83 -4.258980007387567 1105 84-85 -3.2631736077510745 1105 86-87 -3.082965167240719 1105 88-89 -3.2484357405313666 1105 90-91 -8.880526295653898 1105 92-93 -12.032691429974165 1105 94-95 -11.761622810899677 1105 96-97 -12.0361238316781 1105 98-99 -11.321005354639208 1105 100-101 -10.974827675637712 1106 1 0.1453153639644782 1106 2 0.12240320014013406 1106 3 0.03590404672717895 1106 4 0.0510789594189589 1106 5 0.1316267998382301 1106 6 0.0744411510994496 1106 7 -0.016407261147506347 1106 8 0.11660937721681108 1106 9 -0.00961282112086792 1106 10-11 0.22889567910254272 1106 12-13 0.21148290070330233 1106 14-15 0.27600723848674846 1106 16-17 0.07750249235148488 1106 18-19 0.1207017068855265 1106 20-21 0.09689307899440536 1106 22-23 0.1846773620374691 1106 24-25 0.3077061605840967 1106 26-27 0.2068758146768701 1106 28-29 0.13932338384118026 1106 30-31 0.18380049276770904 1106 32-33 0.24615938356760836 1106 34-35 0.2968402835668442 1106 36-37 0.15826542739095117 1106 38-39 0.2510691937588163 1106 40-41 0.1365594519149056 1106 42-43 0.17169125242362782 1106 44-45 0.1412171432802296 1106 46-47 0.1602895123914081 1106 48-49 0.020534692949212285 1106 50-51 -0.04558922558510403 1106 52-53 -0.09890355132279893 1106 54-55 -0.22951594972516887 1106 56-57 0.8354537806680682 1106 58-59 -6.591452280762137 1106 60-61 -8.92897844433039 1106 62-63 -2.6813840448165003 1106 64-65 -0.4519006983421576 1106 66-67 -1.9122744090329427 1106 68-69 -4.660487797462853 1106 70-71 -4.834750813154688 1106 72-73 -6.175484234140519 1106 74-75 -6.8134635106788615 1106 76-77 -8.695013711013862 1106 78-79 -11.924765216064364 1106 80-81 -8.27215778279336 1106 82-83 -4.490170260426968 1106 84-85 -3.5035018972737397 1106 86-87 -3.3701261329192853 1106 88-89 -3.273461711042227 1106 90-91 -8.602520065514469 1106 92-93 -11.958921437203465 1106 94-95 -11.786554675272729 1106 96-97 -12.203648238391956 1106 98-99 -11.39720627767952 1106 100-101 -11.024661659190533 1107 1 0.19298196193605932 1107 2 0.25861740699165736 1107 3 0.19120450981702675 1107 4 0.04944833642537105 1107 5 0.15849790873976843 1107 6 0.15726936310806394 1107 7 0.20123434456112932 1107 8 0.2246851571007653 1107 9 0.37688094639372594 1107 10-11 0.3183120683331353 1107 12-13 0.21438402864824724 1107 14-15 0.20306014149142015 1107 16-17 0.3224323348414515 1107 18-19 0.24590841180897627 1107 20-21 0.2571928799203036 1107 22-23 0.29550059117381267 1107 24-25 0.3450196304152726 1107 26-27 0.49553468126463684 1107 28-29 0.5054844688552436 1107 30-31 0.3105089288781002 1107 32-33 0.507923593712249 1107 34-35 0.5192043221862264 1107 36-37 0.4209377228052986 1107 38-39 0.47469045864068704 1107 40-41 0.44832766337111707 1107 42-43 0.5623122134891005 1107 44-45 0.5570093640223206 1107 46-47 0.4769646420091149 1107 48-49 0.30168543474988496 1107 50-51 0.2777996091267383 1107 52-53 0.15272282249035385 1107 54-55 0.2341324886656082 1107 56-57 0.9866783944706796 1107 58-59 -6.95220451317531 1107 60-61 -8.962609468163336 1107 62-63 -2.69809076000881 1107 64-65 -0.2482218995571408 1107 66-67 -2.0498127667388637 1107 68-69 -4.123470885016507 1107 70-71 -4.717789465824556 1107 72-73 -5.95357043159852 1107 74-75 -6.670689454474951 1107 76-77 -9.365067151539854 1107 78-79 -12.594922053314379 1107 80-81 -8.69764698739636 1107 82-83 -4.695625747783179 1107 84-85 -3.7269818769060024 1107 86-87 -3.47258715200023 1107 88-89 -3.4827079984978653 1107 90-91 -8.766489564897276 1107 92-93 -11.774969989755556 1107 94-95 -11.825031181199982 1107 96-97 -12.297191290025872 1107 98-99 -11.623669890176927 1107 100-101 -11.095889431550606 1108 1 -0.029661963461308005 1108 2 -0.05292010291881866 1108 3 0.020272952152051715 1108 4 0.045777622491826264 1108 5 -0.07056908878780632 1108 6 0.04823696871896743 1108 7 -0.05337955773737946 1108 8 -0.14003752113880807 1108 9 -0.1823760252537383 1108 10-11 -0.15460140395455824 1108 12-13 -0.07481404903543876 1108 14-15 0.053220063616294055 1108 16-17 -0.026731573145994503 1108 18-19 -0.05832830544401446 1108 20-21 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2311 60-61 4.405205798559361 2311 62-63 1.6669673300213645 2311 64-65 1.3704158100472803 2311 66-67 -0.39567945584341757 2311 68-69 -3.205777212712629 2311 70-71 -0.728516663555645 2311 72-73 -1.0262967592519594 2311 74-75 1.251107819871141 2311 76-77 4.424721142775649 2311 78-79 5.400135515400873 2311 80-81 3.3010440853447562 2311 82-83 1.7729072567864073 2311 84-85 1.3985345983222395 2311 86-87 1.452166983993454 2311 88-89 1.6583733854682556 2311 90-91 3.1620934920019614 2311 92-93 5.154877179611127 2311 94-95 8.974567436797212 2311 96-97 9.78205406546975 2311 98-99 10.319472181552955 2311 100-101 9.866924961740954 2312 1 -0.4535194033359602 2312 2 -0.3280990637301038 2312 3 -0.46677315179579537 2312 4 -0.28890212122448844 2312 5 -0.33132096739768 2312 6 -0.2624366193870813 2312 7 -0.29358496552586644 2312 8 -0.24859690342518093 2312 9 -0.4410405020630819 2312 10-11 -0.45582554040461076 2312 12-13 -0.5794131633644071 2312 14-15 -0.498057111674747 2312 16-17 -0.5072024131673913 2312 18-19 -0.5243536937141968 2312 20-21 -0.38245542066653826 2312 22-23 -0.4497109425373864 2312 24-25 -0.4286473099734138 2312 26-27 -0.6518980064852968 2312 28-29 -0.8027348692715677 2312 30-31 -0.659909064672739 2312 32-33 -0.5527408831782736 2312 34-35 -0.6526664009072292 2312 36-37 -0.6863012231646408 2312 38-39 -0.7970120255535349 2312 40-41 -0.7993290091038006 2312 42-43 -0.6946293101411243 2312 44-45 -0.7553174734201775 2312 46-47 -0.6936442893400638 2312 48-49 -0.5668528896707912 2312 50-51 -0.5618042950839097 2312 52-53 -0.5328757274505662 2312 54-55 -0.5650940797281834 2312 56-57 0.5117139449523691 2312 58-59 3.9350153390148 2312 60-61 3.66593640030322 2312 62-63 1.246054926866556 2312 64-65 0.9660356795385105 2312 66-67 -0.7305661031432287 2312 68-69 -1.1611160077574674 2312 70-71 -0.1348667155191201 2312 72-73 6.665390159106952 2312 74-75 5.586520784063772 2312 76-77 5.56612180344289 2312 78-79 5.343139994793937 2312 80-81 2.892547755805655 2312 82-83 1.4149529406210242 2312 84-85 1.0358029442066439 2312 86-87 0.9481853449454327 2312 88-89 1.1376495759244811 2312 90-91 2.5898714677691075 2312 92-93 4.559748644037764 2312 94-95 8.529158434721293 2312 96-97 8.994387752214108 2312 98-99 9.428257179595485 2312 100-101 8.795013984633707 2313 1 -0.28821847490026187 2313 2 -0.3902106700322818 2313 3 -0.33749689795109106 2313 4 -0.3134311983240252 2313 5 -0.3637306203898447 2313 6 -0.3187162571430875 2313 7 -0.3273641610337634 2313 8 -0.383904850766271 2313 9 -0.6736572983607516 2313 10-11 -0.584213018220872 2313 12-13 -0.43556269458142083 2313 14-15 -0.5906824105488653 2313 16-17 -0.522281646908354 2313 18-19 -0.6322836302016626 2313 20-21 -0.7601096225941646 2313 22-23 -0.4819037512978994 2313 24-25 -0.4873792847966598 2313 26-27 -0.6630027137170558 2313 28-29 -0.7282246233816139 2313 30-31 -0.5660383380733762 2313 32-33 -0.5568566437594171 2313 34-35 -0.7500408737760296 2313 36-37 -0.8153206877876471 2313 38-39 -1.0954838443630521 2313 40-41 -1.2591828675045136 2313 42-43 -0.8524034611674267 2313 44-45 -0.8075398414733215 2313 46-47 -0.6800259009492251 2313 48-49 -0.9194430369536803 2313 50-51 -0.7591339724995834 2313 52-53 -0.7465143736577886 2313 54-55 -0.7848015112082578 2313 56-57 0.3878873290348608 2313 58-59 3.850143648029242 2313 60-61 3.6281170543738988 2313 62-63 1.0522204360795833 2313 64-65 1.021619133182348 2313 66-67 1.5409685263099284 2313 68-69 0.021548151885539824 2313 70-71 2.58192473168787 2313 72-73 8.154983062594145 2313 74-75 9.609088276830878 2313 76-77 8.387039853442602 2313 78-79 5.99358709336105 2313 80-81 3.217317644130876 2313 82-83 1.6188250367629067 2313 84-85 1.0907601174708894 2313 86-87 0.9749128231040807 2313 88-89 1.0807793229585663 2313 90-91 2.679292607799127 2313 92-93 4.838262233894021 2313 94-95 8.71212646160998 2313 96-97 9.242467539478506 2313 98-99 9.54096196899979 2313 100-101 8.754006172534941 2314 1 -0.38884247437754027 2314 2 -0.4381021101612994 2314 3 -0.5203694339550573 2314 4 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1.1095196021472127 2314 66-67 1.5641910485033392 2314 68-69 -0.4123973008779096 2314 70-71 2.6229554445798016 2314 72-73 8.312886709471623 2314 74-75 9.744655950034542 2314 76-77 8.840862110116962 2314 78-79 6.47120359949858 2314 80-81 3.361020179629943 2314 82-83 1.8655275811720102 2314 84-85 1.4037058048652717 2314 86-87 1.3953491772364082 2314 88-89 1.404179451857079 2314 90-91 2.7134804135247137 2314 92-93 4.76881147817452 2314 94-95 8.64407161143222 2314 96-97 9.28105244766059 2314 98-99 9.438671792907792 2314 100-101 8.809480052766249 2315 1 -0.7339012492608781 2315 2 -0.584684897094963 2315 3 -0.5771654644352999 2315 4 -0.35356876267015735 2315 5 -0.7398317935114989 2315 6 -0.6630911043112278 2315 7 -0.7626198839989868 2315 8 -0.9008182429124361 2315 9 -1.0396041661698163 2315 10-11 -0.8625488282140381 2315 12-13 -0.6758582635548294 2315 14-15 -0.9685580007149568 2315 16-17 -1.0554312685123506 2315 18-19 -0.9105912250828183 2315 20-21 -0.8739717071266426 2315 22-23 -0.8819767970878871 2315 24-25 -0.9562611512576709 2315 26-27 -0.9914924528213191 2315 28-29 -1.0304962533999884 2315 30-31 -0.836566399569783 2315 32-33 -0.947430601838029 2315 34-35 -0.9390382340955483 2315 36-37 -0.9797191729496362 2315 38-39 -0.9953531143440628 2315 40-41 -0.73438178404421 2315 42-43 -0.6037107613574051 2315 44-45 -0.6123806141911885 2315 46-47 -0.664201925628042 2315 48-49 -0.49362056643846586 2315 50-51 -0.4921218274613679 2315 52-53 -0.5915334658835789 2315 54-55 -0.5823988376696434 2315 56-57 0.5647631637319179 2315 58-59 4.2376515639356676 2315 60-61 3.663108495789924 2315 62-63 1.253665363315946 2315 64-65 1.1837118166903018 2315 66-67 1.7837846452899164 2315 68-69 6.007990790562815 2315 70-71 4.65088089794385 2315 72-73 9.04298004044215 2315 74-75 10.208312988252981 2315 76-77 8.9936177365568 2315 78-79 6.292250324054077 2315 80-81 3.3784902014859277 2315 82-83 1.6802242555178069 2315 84-85 1.2926094613127894 2315 86-87 1.1156811519543481 2315 88-89 1.399793486944798 2315 90-91 2.7371085073282444 2315 92-93 4.603355751315284 2315 94-95 8.884812352172965 2315 96-97 9.340624779162747 2315 98-99 9.388329185547642 2315 100-101 8.701529199201358 2316 1 -0.44370684763898893 2316 2 -0.5367786005174437 2316 3 -0.6506662275715982 2316 4 -0.4351463712029684 2316 5 -0.7827834564327887 2316 6 -0.5838514795135197 2316 7 -0.6366026885057039 2316 8 -0.724673091064929 2316 9 -0.7710498556641028 2316 10-11 -0.7233199154467798 2316 12-13 -0.7053345628678755 2316 14-15 -0.8521883436277662 2316 16-17 -1.041754894547445 2316 18-19 -1.0366576345584875 2316 20-21 -0.803198485157516 2316 22-23 -0.9126792915337418 2316 24-25 -2.2905525593911378 2316 26-27 -1.58305538663938 2316 28-29 -1.2033383161433449 2316 30-31 -0.9295795480119295 2316 32-33 -0.7030052038439152 2316 34-35 -0.9083855927747209 2316 36-37 -0.8336431252031247 2316 38-39 -1.1241975572545613 2316 40-41 -0.7893448543831383 2316 42-43 -1.094439038816887 2316 44-45 -1.019408370877258 2316 46-47 -0.9735702865339917 2316 48-49 -1.138943384597276 2316 50-51 -1.1439894661361407 2316 52-53 -1.07834539688686 2316 54-55 -1.0401668493480543 2316 56-57 0.11818270462298841 2316 58-59 3.4063964028589417 2316 60-61 3.519383853773707 2316 62-63 0.8294750291977451 2316 64-65 0.6313829726947908 2316 66-67 5.7532483279274835 2316 68-69 8.724006298719555 2316 70-71 5.468094734614784 2316 72-73 9.031293956622427 2316 74-75 10.17036581211294 2316 76-77 8.618187273996497 2316 78-79 6.016673433917582 2316 80-81 2.918027884057018 2316 82-83 1.6266334244022964 2316 84-85 1.2444353639739205 2316 86-87 1.2901281018378974 2316 88-89 1.161720847106782 2316 90-91 2.6966904057863808 2316 92-93 4.724796366017095 2316 94-95 8.788278486561648 2316 96-97 9.570580627189967 2316 98-99 9.784595058654817 2316 100-101 8.803031440035413 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 13.0 4 38.0 5 59.0 6 174.0 7 435.0 8 1051.0 9 1752.0 10 2855.0 11 4379.0 12 6061.0 13 8290.0 14 10804.0 15 14985.0 16 20032.0 17 25744.0 18 29431.0 19 31592.0 20 33454.0 21 33666.0 22 32394.0 23 31078.0 24 31833.0 25 33809.0 26 44580.0 27 65089.0 28 90282.0 29 119140.0 30 139261.0 31 148982.0 32 152644.0 33 156983.0 34 156297.0 35 149131.0 36 120694.0 37 70617.0 38 20404.0 39 824.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.40782427147878 19.13808662746032 13.768628884989875 24.68546021607102 2 16.368065975397865 20.33673079202231 38.14208279572537 25.153120436854454 3 19.62220377404901 23.394631581595434 29.650183503419903 27.332981140935658 4 13.535939089699585 16.290766186286238 35.84735529890545 34.32593942510873 5 13.75034890205627 37.26022248064747 34.49770130180321 14.49172731549304 6 33.868479219703296 35.79328264177481 16.40443232498052 13.933805813541378 7 29.350703800185595 31.782867300962625 20.31617901904006 18.550249879811723 8 26.880993708265905 35.34652053967482 19.21486090264443 18.55762484941485 9 26.210938810191962 15.862728217971222 19.392797647663876 38.53353532417294 10-11 25.47510705141822 26.02612837225943 27.282487170600273 21.21627740572208 12-13 25.983251903446885 23.046409445121473 27.641542658452867 23.32879599297877 14-15 22.55812854957505 24.799222520954007 25.2428294356429 27.399819493828048 16-17 22.777998278233063 27.868391042339812 26.268768936641212 23.08484174278591 18-19 22.82778377329833 26.61264828431681 27.937420741035158 22.622147201349705 20-21 23.102450722806704 25.973720693626106 28.234685777534295 22.68914280603289 22-23 22.854108202989615 25.93240946748208 28.188650872622787 23.024831456905513 24-25 22.85374484308442 26.175720850150373 28.112429144818492 22.858105161946714 26-27 22.593290637576978 26.491922083272655 27.863383269274795 23.05140400987557 28-29 22.63246000770902 26.7882873200588 27.80063433108035 22.77861834115183 30-31 22.81172499234077 26.385090334165973 27.81155168134033 22.991632992152926 32-33 22.51776835896054 26.307261520504188 27.844949895296097 23.330020225239174 34-35 23.071300615510093 26.509866860758763 27.667208078524563 22.751624445206573 36-37 23.14077706546932 26.082884373977784 27.719799037684627 23.05653952286827 38-39 22.723268416392518 26.354084220455725 27.815323469656207 23.10732389349555 40-41 22.983254080797327 26.14290842641592 27.979462931732478 22.894374561054278 42-43 22.77203307494985 26.18071000705953 27.742501869727192 23.304755048263427 44-45 22.790495982491215 26.341907206017584 27.935630391987743 22.93196641950346 46-47 23.08457093841024 26.525736537881926 27.394953848080867 22.994738675626966 48-49 22.824195165049005 26.630310638541772 27.32573500733225 23.219759189076974 50-51 22.818601870989983 26.76140250719703 27.464204966250776 22.955790655562215 52-53 23.098707944453018 26.492534392563915 27.37784517251637 23.0309124904667 54-55 22.666016239252922 26.61582523444041 27.575308172601815 23.14285035370485 56-57 22.67433153747989 26.609403136020855 27.621330674496132 23.094934652003126 58-59 21.152776342033146 26.20042256429092 29.272534250607496 23.374266843068433 60-61 22.40705631266784 26.44479846756959 27.90051763265073 23.24762758711184 62-63 22.44288801657374 26.511833833280974 27.71609645057674 23.32918169956855 64-65 21.692213033069752 26.54949377548758 28.166702497642994 23.591590693799674 66-67 21.870721173970896 26.15309221221049 28.738736796967967 23.237449816850646 68-69 22.644256018590518 25.15785064978557 29.478840610872314 22.719052720751602 70-71 21.747473791723635 25.54173871506909 29.438455537619486 23.272331955587788 72-73 21.57608474213592 25.755237189709884 29.34661173635387 23.322066331800325 74-75 22.12993317809914 25.964480947265628 28.559446383136088 23.346139491499137 76-77 20.617130307880988 26.362920840700383 29.12198139403915 23.89796745737948 78-79 21.700568643607426 26.60185261869435 27.860280152779982 23.83729858491824 80-81 22.380529259301394 26.559171672885252 27.019185698589354 24.041113369224004 82-83 22.471024794044464 26.659146035708837 26.86223661254086 24.00759255770584 84-85 22.464918959470232 26.607422165426208 26.76514849138389 24.162510383719667 86-87 22.54544509273499 26.589704758534598 26.78511432479859 24.079735823931816 88-89 22.414539100063223 26.59164305291809 26.573307342836973 24.420510504181717 90-91 22.116407719499957 26.62205480165874 26.883999625713063 24.377537853128235 92-93 22.047433714356597 26.391580581506414 27.152479031399483 24.408506672737502 94-95 20.600906868923172 26.094147834928332 28.59239895435351 24.71254634179499 96-97 20.450715106806523 25.788959592592605 28.857202653857495 24.903122646743384 98-99 20.7528230440494 26.025470874511697 28.059282724673523 25.16242335676538 100-101 20.624101850188612 26.090718744386564 28.006865232620804 25.278314172804023 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 87.0 1 70.5 2 60.5 3 82.5 4 89.5 5 107.5 6 155.0 7 221.0 8 325.0 9 426.0 10 511.5 11 589.5 12 706.5 13 842.0 14 945.0 15 1063.0 16 1177.0 17 1332.0 18 1550.5 19 1842.0 20 2257.5 21 2685.0 22 3247.5 23 4039.5 24 5145.0 25 6535.0 26 8237.5 27 10328.0 28 12674.0 29 15133.0 30 17855.0 31 21220.0 32 25132.0 33 28669.0 34 31558.5 35 34942.5 36 38594.0 37 41931.0 38 45322.0 39 48256.0 40 51042.5 41 54212.0 42 56930.5 43 59712.5 44 62574.0 45 65449.5 46 67915.0 47 69890.0 48 71399.0 49 71985.0 50 72205.5 51 72014.0 52 71341.5 53 70386.0 54 68193.5 55 64073.0 56 58840.0 57 52541.5 58 45581.0 59 38448.0 60 31582.0 61 25401.0 62 19683.5 63 14576.0 64 10576.5 65 7617.0 66 5487.0 67 4037.5 68 2872.0 69 1982.0 70 1386.5 71 954.5 72 678.5 73 481.5 74 344.0 75 228.0 76 137.0 77 82.0 78 46.5 79 30.0 80 17.5 81 7.5 82 4.5 83 3.0 84 3.0 85 2.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02090716992945228 2 0.004024909719038941 3 0.0036335990519101554 4 0.0 5 0.0619947899779748 6 1.1180304775108171E-4 7 0.0 8 2.795076193777042E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 8.385228581331127E-5 16-17 0.0 18-19 0.0013695873349507507 20-21 0.0 22-23 0.0 24-25 0.0 26-27 8.385228581331127E-5 28-29 0.0025994208602126492 30-31 0.009391456011090862 32-33 3.354091432532451E-4 34-35 0.0010341781916975055 36-37 0.025994208602126496 38-39 0.024960030410428987 40-41 0.02705633755576177 42-43 0.02781100812808157 44-45 0.027978712699708193 46-47 0.029460103082410028 48-49 0.029488053844347797 50-51 0.025071833458180072 52-53 0.021913397359212012 54-55 0.016351195733595697 56-57 0.14621043569647707 58-59 0.7683943964312467 60-61 0.3101975559853762 62-63 3.354091432532451E-4 64-65 0.15071050836845812 66-67 1.4432655434187136 68-69 2.7659235490759477 70-71 2.2218899187191843 72-73 1.9023568082465927 74-75 1.5936965441677942 76-77 1.1340742148630971 78-79 0.4398052390908176 80-81 8.385228581331127E-5 82-83 1.6770457162662255E-4 84-85 1.1180304775108171E-4 86-87 0.002962780765403665 88-89 5.5901523875540854E-5 90-91 0.23104099817761034 92-93 0.18299363840658295 94-95 1.3106112272620551 96-97 1.3055521393513188 98-99 1.0377279384636027 100-101 0.41478930715651313 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1788860.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.211081487274306 #Duplication Level Percentage of deduplicated Percentage of total 1 77.81155839741922 46.073065249213926 2 12.94120343787443 15.325253026067543 3 4.07658657330741 7.2413729934610025 4 1.724632570052247 4.084694385638836 5 0.9398889925666948 2.782592186392936 6 0.566255695850255 2.0117167289773596 7 0.3722773045097112 1.543005926923114 8 0.2706334652735098 1.2819600132394566 9 0.17902655582845492 0.9540320386990236 >10 1.0247924361223217 11.06106668999728 >50 0.0643571600994969 2.56136884885787 >100 0.0250125835532278 2.7315470638032546 >500 0.0025480085916074037 1.0458667537631154 >1k 0.0012268189515146756 1.3024580949652431 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3588 0.20057466766544058 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3171 0.17726373220934002 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2056 0.11493353308811198 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1822 0.10185257650123541 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.590152387554085E-5 2 1.118030477510817E-4 0.0 0.0 0.0 5.590152387554085E-5 3 1.118030477510817E-4 0.0 0.0 0.0 5.590152387554085E-5 4 1.118030477510817E-4 0.0 0.0 0.0 5.590152387554085E-5 5 1.118030477510817E-4 0.0 0.0 0.0 5.590152387554085E-5 6 1.6770457162662255E-4 0.0 0.0 0.0 5.590152387554085E-5 7 1.6770457162662255E-4 0.0 0.0 0.0 5.590152387554085E-5 8 2.236060955021634E-4 0.0 0.0 0.0 5.590152387554085E-5 9 2.236060955021634E-4 0.0 0.0 0.0 5.590152387554085E-5 10-11 2.236060955021634E-4 0.0 0.0 0.0 5.590152387554085E-5 12-13 3.9131066712878594E-4 0.0 0.0 0.0 1.6770457162662255E-4 14-15 4.472121910043268E-4 0.0 0.0 0.0 1.6770457162662255E-4 16-17 4.751629529420972E-4 0.0 0.0 0.0 1.6770457162662255E-4 18-19 5.31064476817638E-4 0.0 0.0 2.7950761937770423E-5 2.236060955021634E-4 20-21 5.590152387554085E-4 0.0 0.0 8.385228581331127E-5 3.074583813154747E-4 22-23 6.149167626309494E-4 0.0 0.0 2.5155685743993384E-4 4.4721219100432683E-4 24-25 7.26719810382031E-4 0.0 0.0 5.31064476817638E-4 5.031137148798677E-4 26-27 7.26719810382031E-4 0.0 0.0 0.001257784287199669 5.31064476817638E-4 28-29 0.0010341781916975057 0.0 0.0 0.0056740046733673956 5.590152387554085E-4 30-31 0.001118030477510817 0.0 0.0 0.023310935456100534 5.590152387554085E-4 32-33 0.0011459812394485874 0.0 0.0 0.06062520264302405 6.708182865064902E-4 34-35 0.0013416365730129804 0.0 0.0 0.12083114385698154 6.708182865064902E-4 36-37 0.0015093411446396028 0.0 0.0 0.2223483112149637 6.708182865064902E-4 38-39 0.0015652426685151437 0.0 0.0 0.37644086177789204 7.546705723198014E-4 40-41 0.0015652426685151437 0.0 0.0 0.543726171975448 8.664736200708831E-4 42-43 0.001649094954328455 0.0 0.0 0.7130518877944613 8.944243820086536E-4 44-45 0.0018167995259550777 0.0 0.0 0.9181545788938206 9.22375143946424E-4 46-47 0.0019286025737061591 0.0 0.0 1.1522981116465236 9.503259058841944E-4 48-49 0.0019565533356439295 0.0 0.0 1.3716277405722082 9.503259058841944E-4 50-51 0.0019565533356439295 0.0 0.0 1.5982804691255885 9.503259058841944E-4 52-53 0.0019565533356439295 0.0 0.0 1.8107901121384569 9.503259058841944E-4 54-55 0.0019565533356439295 0.0 0.0 2.039259640217792 9.503259058841944E-4 56-57 0.0019565533356439295 2.7950761937770423E-5 0.0 2.243607660744832 9.503259058841944E-4 58-59 0.0019565533356439295 1.118030477510817E-4 0.0 2.379476314524334 9.503259058841944E-4 60-61 0.0019565533356439295 1.118030477510817E-4 2.7950761937770423E-5 2.4833972474089645 9.78276667821965E-4 62-63 0.0020124548595194707 1.118030477510817E-4 5.590152387554085E-5 2.570966984559999 0.0010062274297597353 64-65 0.0020683563833950114 1.118030477510817E-4 5.590152387554085E-5 2.659375244569167 0.0010062274297597353 66-67 0.002124257907270552 1.118030477510817E-4 5.590152387554085E-5 2.7343112373243295 0.0010062274297597353 68-69 0.002124257907270552 1.118030477510817E-4 5.590152387554085E-5 2.8236418724774435 0.0010062274297597353 70-71 0.0022081101930838632 1.118030477510817E-4 5.590152387554085E-5 2.9815357266639086 0.0010062274297597353 72-73 0.0022919624788971748 1.118030477510817E-4 5.590152387554085E-5 3.1866943192871435 0.0010341781916975057 74-75 0.0023478640027727155 1.118030477510817E-4 5.590152387554085E-5 3.4185179387990114 0.0010900797155730464 76-77 0.0023478640027727155 1.118030477510817E-4 5.590152387554085E-5 3.7564146998647185 0.0011739320013863578 78-79 0.0023478640027727155 1.118030477510817E-4 5.590152387554085E-5 4.238146081862192 0.0013416365730129804 80-81 0.0025994208602126492 1.118030477510817E-4 5.590152387554085E-5 4.7700770322999 0.0013416365730129804 82-83 0.00262737162215042 1.118030477510817E-4 5.590152387554085E-5 5.277746721375625 0.0013975380968885211 84-85 0.0026553223840881904 1.118030477510817E-4 5.590152387554085E-5 5.684933421285065 0.0013975380968885211 86-87 0.002711223907963731 1.118030477510817E-4 5.590152387554085E-5 6.043877106089912 0.0014254888588262917 88-89 0.0027391746699015015 1.118030477510817E-4 5.590152387554085E-5 6.411206019476091 0.0014813903827018324 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2075 0.0 49.059826 1 GTATCAA 3700 0.0 43.817444 1 ATCAACG 4720 0.0 33.24356 3 CAACGCA 4775 0.0 32.95933 5 TCAACGC 4775 0.0 32.860653 4 TATCAAC 4930 0.0 32.305397 2 AACGCAG 4975 0.0 31.617533 6 CGCAGAG 5945 0.0 26.141872 8 ACGCAGA 5955 0.0 26.097971 7 GTACATG 5140 0.0 22.37263 1 TACATGG 4860 0.0 22.29959 2 GCAGAGT 7065 0.0 21.664356 9 TAAGGTG 930 0.0 21.28 5 ACATGGG 4975 0.0 20.647558 3 AGAGTAC 6335 0.0 20.183563 10-11 TGGTATC 1125 0.0 20.104534 2 GAGTACT 4445 0.0 19.018106 12-13 CAGAGTA 6770 0.0 18.538864 10-11 GTACTTT 4560 0.0 18.486843 14-15 AGTACTT 4805 0.0 18.27932 12-13 >>END_MODULE