FastQCFastQC Report
Fri 27 May 2016
SRR939782_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939782_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915512
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33490.1748357619268373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26720.13949273092520434No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20250.048.3114551
GTATCAA35350.040.714011
TATCAAC45950.031.3226912
ATCAACG46100.031.2207743
TCAACGC46300.031.0867234
CAACGCA47400.030.2644885
AACGCAG49750.029.0255076
TACATGG53950.026.939542
GTACATG56600.026.6747511
ACATGGG57250.024.6472743
ACGCAGA60300.023.4792217
CGCAGAG61250.022.6536988
CATGGGG35850.021.3861684
GTGTAGC14050.020.420451
GTAAGGT9500.019.30974
GTGTAAG8800.019.2411771
TGGTATC10800.019.1624952
GAGTACA38650.018.9839651
GTGGTAT13250.018.8135951
GCAGAGT67600.018.7862579
Produced by FastQC (version 0.11.5)