FastQCFastQC Report
Fri 27 May 2016
SRR939782_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939782_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915512
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38320.20005095243464935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33540.17509678874368836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22010.11490400477783486No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA19580.10221810147887354No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23250.058.5772861
GTATCAA41600.040.1531521
ATCAACG53500.030.4116923
TCAACGC53850.030.2140314
CAACGCA54250.029.9912575
TATCAAC54900.029.8151992
AACGCAG57250.028.419666
TGGTATC10150.025.7091432
ACGCAGA67000.024.3547637
CGCAGAG68100.023.9613698
TACATGG56750.023.0745982
GTACATG57350.023.0028531
GTGGTAT13100.022.0967751
ACATGGG56400.020.9421123
TAAGGTG10950.020.793495
GCAGAGT78350.020.4028649
GAGTACA40450.020.2954121
AGAGTAC69850.019.999510-11
GAGTACT48700.019.3344512-13
CAGAGTA74950.019.0816510-11
Produced by FastQC (version 0.11.5)