##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939782_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1915512 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.36887317855487 31.0 31.0 34.0 30.0 34.0 2 31.605074778962493 31.0 31.0 34.0 30.0 34.0 3 31.653868521836458 31.0 31.0 34.0 30.0 34.0 4 35.46706050392793 37.0 35.0 37.0 33.0 37.0 5 35.19031413011247 37.0 35.0 37.0 32.0 37.0 6 35.21359563396105 37.0 35.0 37.0 32.0 37.0 7 35.11482517467915 37.0 35.0 37.0 32.0 37.0 8 35.07169310346268 37.0 35.0 37.0 32.0 37.0 9 36.6665805278171 39.0 35.0 39.0 32.0 39.0 10-11 36.52983458208563 38.0 35.0 39.0 32.0 39.0 12-13 36.45136052397479 38.0 35.0 39.0 32.0 39.0 14-15 37.50169824047043 39.0 36.0 41.0 32.0 41.0 16-17 37.40688520875881 39.0 36.0 41.0 32.0 41.0 18-19 37.3588079844971 39.0 36.0 41.0 32.0 41.0 20-21 37.25842046408479 39.0 36.0 40.0 31.0 41.0 22-23 37.13545908352441 39.0 36.0 40.0 31.0 41.0 24-25 36.97512231716638 39.0 36.0 40.0 31.0 41.0 26-27 36.672981166393114 38.5 35.5 40.0 30.0 41.0 28-29 36.44571712419447 38.0 35.0 40.0 30.0 41.0 30-31 36.18226484616123 38.0 35.0 40.0 29.5 41.0 32-33 36.183483058315474 38.0 35.0 40.0 30.0 41.0 34-35 36.04395012926048 38.0 35.0 40.0 29.5 41.0 36-37 36.1142034088014 38.0 35.0 40.0 30.0 41.0 38-39 36.02121260529822 38.0 35.0 40.0 29.0 41.0 40-41 35.946025135838354 38.0 35.0 40.0 29.0 41.0 42-43 35.73799407155893 38.0 34.5 40.0 28.0 41.0 44-45 35.52033033465726 38.0 34.0 40.0 27.5 41.0 46-47 35.269540728536285 38.0 34.0 40.0 27.0 41.0 48-49 34.97605888138524 38.0 33.5 40.0 26.0 41.0 50-51 34.74831716011176 38.0 33.0 40.0 26.0 41.0 52-53 34.49231484845827 38.0 33.0 40.0 24.5 41.0 54-55 34.208451839508186 37.0 33.0 40.0 24.0 41.0 56-57 33.824459726694485 37.0 32.5 40.0 23.5 41.0 58-59 33.441394520107416 36.5 32.0 39.0 23.0 41.0 60-61 33.119045456254 36.0 31.0 39.0 22.0 40.0 62-63 32.81009150556092 36.0 31.0 39.0 22.0 40.0 64-65 32.35351566578544 35.0 31.0 38.5 20.5 40.0 66-67 31.389705728807755 34.5 29.5 38.0 16.0 40.0 68-69 29.389743838723014 34.0 26.0 37.0 8.5 39.0 70-71 28.720454635627448 33.0 25.0 36.0 8.5 39.0 72-73 29.309219414965817 33.0 26.5 36.0 9.0 39.0 74-75 29.448215672885368 33.0 27.5 36.0 8.0 38.5 76-77 29.037906836396743 32.5 27.5 35.0 9.0 37.0 78-79 29.58871518424317 34.0 29.0 35.0 8.0 37.0 80-81 29.41347744101838 34.0 29.0 35.0 7.0 37.0 82-83 28.963847263812497 33.5 28.5 35.0 6.5 36.5 84-85 28.606511992616074 33.0 28.0 35.0 2.0 36.0 86-87 28.217725600257268 33.0 27.0 35.0 2.0 35.5 88-89 27.74872253475833 33.0 26.5 35.0 2.0 35.0 90-91 27.23616375151918 32.0 26.0 35.0 2.0 35.0 92-93 26.720531116484782 32.0 25.0 34.0 2.0 35.0 94-95 25.872938671227324 32.0 23.5 34.0 2.0 35.0 96-97 25.33320673532716 32.0 21.5 34.0 2.0 35.0 98-99 24.637453850458783 31.0 18.0 34.0 2.0 35.0 100-101 23.11331174119504 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3145379225702847 1101 2 0.41880919057495447 1101 3 0.41074555961821346 1101 4 0.3223636139321684 1101 5 0.3870249496219671 1101 6 0.30281271053978287 1101 7 0.31498853358840506 1101 8 0.2900767503580539 1101 9 0.4694905932766318 1101 10-11 0.4723764200626164 1101 12-13 0.4207341603623149 1101 14-15 0.4858185730006639 1101 16-17 0.5156828818609256 1101 18-19 0.40030644830832074 1101 20-21 0.529298379346308 1101 22-23 0.4524301572839491 1101 24-25 0.3902462754614362 1101 26-27 0.5202984763390575 1101 28-29 0.4603779190249213 1101 30-31 0.17939977726189937 1101 32-33 0.060221249441916314 1101 34-35 0.18239112849700945 1101 36-37 0.4751433333282691 1101 38-39 0.5052479282137909 1101 40-41 0.557860938389922 1101 42-43 0.3787390539238231 1101 44-45 0.2798084917235215 1101 46-47 0.20856675248061407 1101 48-49 0.29350307351970883 1101 50-51 0.18592848367043757 1101 52-53 -0.020562938669691277 1101 54-55 -0.08026491201263752 1101 56-57 0.029681756570852258 1101 58-59 -0.05791209938507791 1101 60-61 -0.25076779188111686 1101 62-63 0.025313790120726765 1101 64-65 -0.0956930622219403 1101 66-67 -0.49379572406254724 1101 68-69 -8.807555977767464 1101 70-71 -6.728446877358692 1101 72-73 -1.7967578752981161 1101 74-75 -0.6160500388721211 1101 76-77 -0.5390088653594134 1101 78-79 -0.5367379067374429 1101 80-81 -0.5759852152549314 1101 82-83 -0.5861962611123026 1101 84-85 -0.2976598239126993 1101 86-87 -0.3342896414552854 1101 88-89 -0.18704077920268602 1101 90-91 -0.48817552143350085 1101 92-93 -0.6979434026118874 1101 94-95 -0.33858935748723695 1101 96-97 -0.7393698908049622 1101 98-99 -0.5916726002101278 1101 100-101 -0.43894525221100267 1102 1 0.08072196256107134 1102 2 0.044690967127795744 1102 3 0.10135373657081104 1102 4 0.1689798757070804 1102 5 0.1314275960251834 1102 6 0.14353126815439055 1102 7 0.15983270263965466 1102 8 0.07739902870860504 1102 9 0.28363873954516094 1102 10-11 0.18655918838229724 1102 12-13 0.17741104366526628 1102 14-15 0.3278544276846702 1102 16-17 0.36208211534481194 1102 18-19 0.1883570340773133 1102 20-21 0.225650669330264 1102 22-23 0.21049338656732886 1102 24-25 0.13821311441692785 1102 26-27 0.2611837760942848 1102 28-29 -0.01582692347524528 1102 30-31 -0.19063016507860908 1102 32-33 -0.15084724219803292 1102 34-35 -0.07331260434080633 1102 36-37 0.028604757677989312 1102 38-39 0.10240072198641315 1102 40-41 0.0023019398772845534 1102 42-43 0.04945809463681883 1102 44-45 0.0780180510206705 1102 46-47 -0.006869997740928113 1102 48-49 -0.08453703047819516 1102 50-51 0.13807075663371648 1102 52-53 -0.06253625895123349 1102 54-55 -0.18448240973866348 1102 56-57 -0.13231247845504868 1102 58-59 -0.3145226176569338 1102 60-61 -0.1884276047317357 1102 62-63 -0.25482447361390825 1102 64-65 -0.16218630958002933 1102 66-67 0.1730314467571361 1102 68-69 -4.745529194597637 1102 70-71 -0.05265554755962398 1102 72-73 0.30983851600572265 1102 74-75 0.10005938925650071 1102 76-77 -0.17100110455460538 1102 78-79 -0.2548312101154231 1102 80-81 -0.19418389751868403 1102 82-83 -0.10207031297071723 1102 84-85 -0.07508590497953804 1102 86-87 -0.18679982555968877 1102 88-89 -0.4077831940700172 1102 90-91 -0.5954841168761753 1102 92-93 -0.8993162559959167 1102 94-95 -0.5194146700860536 1102 96-97 -0.9114019587783666 1102 98-99 -0.7430864925582412 1102 100-101 -0.8354674073958712 1103 1 0.28770196949174576 1103 2 0.1261985812432691 1103 3 0.21207702941104145 1103 4 0.23542241849436607 1103 5 0.19600727952440167 1103 6 0.1163586943715913 1103 7 0.07649640130935609 1103 8 0.166539288223845 1103 9 0.30270242473854125 1103 10-11 0.2965375540908539 1103 12-13 0.3514721539207386 1103 14-15 0.3288331447399031 1103 16-17 0.311566526141263 1103 18-19 0.32579711262494726 1103 20-21 0.39409576780359146 1103 22-23 0.22696203970634343 1103 24-25 0.28898535514234425 1103 26-27 0.26567451594373637 1103 28-29 0.03517333161205727 1103 30-31 0.23580650588764485 1103 32-33 0.3216734776294672 1103 34-35 -0.07177413910005725 1103 36-37 0.03303220699743292 1103 38-39 0.17142903186454816 1103 40-41 0.18558433596530222 1103 42-43 0.08510353771865908 1103 44-45 0.08688040976006306 1103 46-47 0.24297622792951046 1103 48-49 0.12095312218915666 1103 50-51 -0.07429688674460522 1103 52-53 -0.008912214325398793 1103 54-55 -0.05180716957156051 1103 56-57 0.052099882209525106 1103 58-59 0.1104787059092871 1103 60-61 -0.010151417372512128 1103 62-63 -0.08341470713912003 1103 64-65 -0.06402883407803728 1103 66-67 0.0767926609727212 1103 68-69 -4.476795558689041 1103 70-71 0.1615392188189091 1103 72-73 0.4772407646243977 1103 74-75 0.35680283880696706 1103 76-77 0.19185240794948655 1103 78-79 -0.12218653229550469 1103 80-81 -0.07313378008225868 1103 82-83 -0.1914503786196029 1103 84-85 -0.3310725153023917 1103 86-87 -0.4217360777977177 1103 88-89 -0.5337191960337044 1103 90-91 -0.4474071382650031 1103 92-93 -0.3726886305218393 1103 94-95 -0.4404226740519377 1103 96-97 -0.6799220192292488 1103 98-99 -0.39644341745668754 1103 100-101 -0.5240805265842106 1104 1 0.1316865681072521 1104 2 0.19011600564829934 1104 3 0.2037796594216772 1104 4 0.20165520675170967 1104 5 0.2662394634296916 1104 6 0.2310939725833805 1104 7 0.15010096560629904 1104 8 0.07204356845134186 1104 9 0.2907650327334963 1104 10-11 0.22535423516684716 1104 12-13 0.1625351540250648 1104 14-15 0.20124675177436302 1104 16-17 0.2621413201039786 1104 18-19 0.2429258168293913 1104 20-21 0.240733160168368 1104 22-23 0.13407013118894184 1104 24-25 0.21563812011294914 1104 26-27 0.33138353242072327 1104 28-29 0.30599631761483437 1104 30-31 0.2567965880387604 1104 32-33 0.25444698590513326 1104 34-35 0.24882958941636701 1104 36-37 0.3931076790575432 1104 38-39 0.45516188907078003 1104 40-41 0.2987401448797371 1104 42-43 0.33884446494339926 1104 44-45 0.3735197926494678 1104 46-47 0.34813240617020114 1104 48-49 0.319793581640603 1104 50-51 0.18241591772472532 1104 52-53 0.09569093382119576 1104 54-55 0.10286606662577924 1104 56-57 0.17162093320652616 1104 58-59 0.1777584381717361 1104 60-61 0.13971302310420697 1104 62-63 0.16437539084025587 1104 64-65 0.1040122138972066 1104 66-67 0.3134412170629446 1104 68-69 -4.336791050030861 1104 70-71 0.5843723349132581 1104 72-73 1.0338873781544535 1104 74-75 0.659148779995359 1104 76-77 0.5187128589831786 1104 78-79 0.1950050053368031 1104 80-81 0.27879804804673114 1104 82-83 0.10927100668960676 1104 84-85 0.14989435205552581 1104 86-87 0.21744763923121724 1104 88-89 0.18395582324200532 1104 90-91 0.14757326226212442 1104 92-93 -0.038687951824890376 1104 94-95 0.3598538409951111 1104 96-97 0.1135965448110845 1104 98-99 0.5029879640033776 1104 100-101 0.4688485543809797 1105 1 0.26416301196529446 1105 2 0.2539930676395663 1105 3 0.3066278560669353 1105 4 0.23916178327836946 1105 5 0.24666588758829278 1105 6 0.18546698304843545 1105 7 0.19054726763319252 1105 8 0.22918723162129595 1105 9 0.32550283728514984 1105 10-11 0.22077791297756022 1105 12-13 0.16942790853724432 1105 14-15 0.42956772292541245 1105 16-17 0.5420190051473028 1105 18-19 0.4083339617401194 1105 20-21 0.38178259780601564 1105 22-23 0.28735431960335234 1105 24-25 0.3814873926321525 1105 26-27 0.48202059350538917 1105 28-29 0.49213733956487715 1105 30-31 0.6351252991166874 1105 32-33 0.4833562879469824 1105 34-35 0.5120361711994192 1105 36-37 0.45155341011412986 1105 38-39 0.5287990953092105 1105 40-41 0.4755689015292006 1105 42-43 0.41595041224535123 1105 44-45 0.3862113437838559 1105 46-47 0.3891617344651124 1105 48-49 0.6080714985441702 1105 50-51 0.5703533501812004 1105 52-53 0.4763912935228518 1105 54-55 0.5954942082283452 1105 56-57 0.5641645866352647 1105 58-59 0.5177236350238701 1105 60-61 0.4485760109667467 1105 62-63 0.4366347936458297 1105 64-65 0.33444647443038633 1105 66-67 1.0087240330267804 1105 68-69 2.3901874898765385 1105 70-71 2.564376318202733 1105 72-73 1.728308192915577 1105 74-75 1.2327301671349282 1105 76-77 0.8492723568379432 1105 78-79 0.851042654655906 1105 80-81 0.7805190364619179 1105 82-83 0.6264273623751748 1105 84-85 0.5976890274680677 1105 86-87 0.41395317074719173 1105 88-89 0.8045086806735178 1105 90-91 0.8633365034445148 1105 92-93 0.6741239901348202 1105 94-95 0.7452051492838727 1105 96-97 0.4544460463935529 1105 98-99 0.7860251547137267 1105 100-101 0.7795417576642514 1106 1 0.4330810459790264 1106 2 0.3737432552535118 1106 3 0.43912903580005747 1106 4 0.1961997488571896 1106 5 0.44452064425144755 1106 6 0.38676219489108377 1106 7 0.371703863432721 1106 8 0.40266101036365143 1106 9 0.4883902003165801 1106 10-11 0.41987736603873316 1106 12-13 0.48641674108998245 1106 14-15 0.6709099369986475 1106 16-17 0.5513451332735997 1106 18-19 0.5082222768733331 1106 20-21 0.5337563900607307 1106 22-23 0.5997853286025858 1106 24-25 0.550796765429908 1106 26-27 0.5300812058024675 1106 28-29 0.5537017384414895 1106 30-31 0.7041568369689699 1106 32-33 0.7136355157051142 1106 34-35 0.8984322892018426 1106 36-37 0.85105236134698 1106 38-39 0.8296203870494168 1106 40-41 0.7022226586933513 1106 42-43 0.7063417198469892 1106 44-45 0.8750259666538369 1106 46-47 0.8715665606649523 1106 48-49 1.0879775772360105 1106 50-51 0.9327876333296246 1106 52-53 0.8994061716725312 1106 54-55 0.9156386969517598 1106 56-57 0.7535730778033738 1106 58-59 0.9115759781713422 1106 60-61 1.0966808502631906 1106 62-63 1.0155399701276693 1106 64-65 1.0505114772056103 1106 66-67 1.5198018309691577 1106 68-69 2.916552446293977 1106 70-71 3.256014089203539 1106 72-73 2.352537988296845 1106 74-75 1.798439223119761 1106 76-77 1.4743290048159778 1106 78-79 1.2915108268075528 1106 80-81 1.2819240622289527 1106 82-83 1.302792786528208 1106 84-85 1.2871261024783038 1106 86-87 1.2668409703318417 1106 88-89 1.5657278387078648 1106 90-91 1.4259852693501252 1106 92-93 1.376094820064079 1106 94-95 1.5825568511138606 1106 96-97 1.3550150727343429 1106 98-99 1.5590190367320993 1106 100-101 1.9182297800989296 1107 1 0.2488537748722166 1107 2 0.13154956879594337 1107 3 0.15869483796737427 1107 4 0.09022412711229322 1107 5 0.19412525112156231 1107 6 0.24897498121350736 1107 7 0.2245984436300077 1107 8 0.2280657751749544 1107 9 0.026263136521087915 1107 10-11 0.14387486620348255 1107 12-13 0.06622741151900158 1107 14-15 0.21623889652055084 1107 16-17 0.22440389884760492 1107 18-19 0.0751786194220685 1107 20-21 0.1918168193107448 1107 22-23 0.25031573336199386 1107 24-25 0.21371358919151362 1107 26-27 0.26459294930251787 1107 28-29 0.370236355326071 1107 30-31 0.20372444325248296 1107 32-33 0.33925277731599834 1107 34-35 0.4375571686123365 1107 36-37 0.14783906295804172 1107 38-39 0.37409927834979584 1107 40-41 0.25974317646389977 1107 42-43 0.28006538866310393 1107 44-45 0.47048437421340594 1107 46-47 0.4525983854906741 1107 48-49 0.4921285028988578 1107 50-51 0.28326502192005165 1107 52-53 0.3507498601286656 1107 54-55 0.46499815467980454 1107 56-57 0.4058642513539539 1107 58-59 0.5518121175355333 1107 60-61 0.39092815030067385 1107 62-63 0.623660140470804 1107 64-65 0.3728593723853848 1107 66-67 0.7124019027016786 1107 68-69 2.2051401420054724 1107 70-71 2.5335194340328044 1107 72-73 1.6409450023116925 1107 74-75 1.0824489739896812 1107 76-77 0.6111357029484452 1107 78-79 0.5959459817940953 1107 80-81 0.6114030777648765 1107 82-83 0.627152892441714 1107 84-85 0.4922835521277982 1107 86-87 0.48518035159560924 1107 88-89 0.6423891380388262 1107 90-91 0.4833136271398537 1107 92-93 0.7477686833898538 1107 94-95 1.095875711906313 1107 96-97 0.7372661074003837 1107 98-99 0.9234996619285809 1107 100-101 1.1289812052967072 1108 1 -0.07703461628206654 1108 2 -0.08450943211807171 1108 3 -0.0589110014636951 1108 4 0.12144466443947266 1108 5 0.025450196193119723 1108 6 0.06126800352726036 1108 7 -0.06623135889490328 1108 8 0.01139374503807744 1108 9 -0.06210303393034167 1108 10-11 -0.0026860270045148127 1108 12-13 -0.002051063624136873 1108 14-15 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-0.0401790039273493 2316 78-79 -0.2850655399343651 2316 80-81 -0.21536783586113728 2316 82-83 0.04870958159754224 2316 84-85 -0.20930728938275678 2316 86-87 -0.14275399389457988 2316 88-89 -0.16316126916232676 2316 90-91 -0.152596866553651 2316 92-93 -0.2741452630691832 2316 94-95 0.03622976471976713 2316 96-97 0.09471986603590565 2316 98-99 -0.10490279888859178 2316 100-101 -0.3056098180902964 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 18.0 4 40.0 5 73.0 6 187.0 7 481.0 8 1097.0 9 1908.0 10 2962.0 11 4522.0 12 6315.0 13 7900.0 14 9796.0 15 11688.0 16 14187.0 17 16711.0 18 16271.0 19 15184.0 20 15174.0 21 16065.0 22 17327.0 23 20090.0 24 23664.0 25 29061.0 26 36015.0 27 44549.0 28 54450.0 29 65479.0 30 77834.0 31 92875.0 32 110650.0 33 134005.0 34 165891.0 35 208664.0 36 259854.0 37 275638.0 38 147771.0 39 11110.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.46741006273873 19.121762219431513 13.789570697906358 24.621257019923398 2 16.393375852586924 20.375770082723516 38.086602257690906 25.144251806998653 3 19.689357205801894 23.396929906990923 29.685640183929934 27.228072703277245 4 13.605291953274111 16.32143259869946 35.82013581747334 34.253139630553086 5 13.864857019950803 37.17110621076767 34.472872004978306 14.491164764303225 6 33.91556295712369 35.791564648579225 16.362117660570814 13.930754733726266 7 29.303653540150098 31.813217562719526 20.379251082739238 18.50387781439114 8 26.941413053011416 35.299387317855484 19.198209147214946 18.56099048191815 9 26.20787549229658 15.850696837190265 19.47056452791734 38.47086314259582 10-11 25.442779789424446 25.973342897355906 27.339635564799387 21.244241748420265 12-13 25.99234564962266 23.05931782207577 27.660672446844497 23.28766408145707 14-15 22.534758330931886 24.79329286373565 25.262958415295756 27.40899039003671 16-17 22.74945771480628 27.877974737732348 26.30267214128911 23.069895406172265 18-19 22.856578293427553 26.617296054527458 27.946810043476628 22.57931560856836 20-21 23.091763455410355 25.96201433350457 28.240752341932602 22.705469869152477 22-23 22.86161611099278 25.903622634574987 28.14897531312777 23.085785941304465 24-25 22.81477966317595 26.215074258272143 28.128715556505696 22.84143052204621 26-27 22.620184953740683 26.46548744822517 27.830087715726286 23.084239882307863 28-29 22.63351068369989 26.764809295062964 27.81134438503762 22.790335636199522 30-31 22.78962163211918 26.384002952748453 27.781781656885514 23.044593758246855 32-33 22.5260582191164 26.337523861630864 27.830238053783653 23.306179865469083 34-35 23.08332854763682 26.513942039124373 27.651127945161143 22.751601468077666 36-37 23.163182649594983 26.070081003396915 27.729396394042332 23.03733995296577 38-39 22.718063814888747 26.359463018509032 27.829551749004526 23.092921417597694 40-41 23.021540812465638 26.166301887029103 27.947555349300018 22.864601951205234 42-43 22.743277618890215 26.172191729455925 27.738397760099087 23.346132891554774 44-45 22.79190934198734 26.371719800477123 27.910601416727417 22.925769440808125 46-47 23.10101033853003 26.525527573332496 27.359925589756052 23.01353649838142 48-49 22.799240736845817 26.611311348841703 27.378543369576434 23.210904544736042 50-51 22.802413639492976 26.776976105282547 27.43379016908279 22.98682008614169 52-53 23.121188270786604 26.47515732791105 27.420078257101142 22.98357614420121 54-55 22.67596451508088 26.663264165392903 27.513375800171136 23.147395519355076 56-57 22.635270193099334 26.74119320978451 27.397565388503033 23.22597120861313 58-59 22.576090035360966 26.450758975014978 27.41149642539672 23.56165456422734 60-61 22.36429540117813 26.58918644461587 27.630339981021525 23.41617817318448 62-63 22.42262544757257 26.60676496622372 27.664624610702898 23.30598497550081 64-65 22.200645660974995 26.673258844164003 27.488127162895086 23.637968331965908 66-67 22.028710706064576 26.721392571737546 27.62409285484945 23.62580386734842 68-69 22.23215851720067 26.232841211451962 28.045284336650063 23.489715934697305 70-71 22.030415179227905 26.271958370674938 27.776334645084162 23.921291805013 72-73 22.114738058678558 26.471756703070398 27.5740425875812 23.839462650669844 74-75 22.354932363609016 26.73404499483918 26.992699651184378 23.918322990367425 76-77 22.408377209428146 26.50316315222716 27.151199407581327 23.937260230763364 78-79 22.467182063844092 26.596467816912877 27.036737711285696 23.89961240795733 80-81 22.638111596049033 26.550420782258232 26.84656065320442 23.964906968488318 82-83 22.47922942735109 26.62444293570826 26.865318670923493 24.031008966017154 84-85 22.433802687490424 26.638334705034627 26.746922286008722 24.180940321466224 86-87 22.53937377837919 26.581294166290782 26.76649501328121 24.112837042048817 88-89 22.405483875935687 26.608471440843452 26.545798722165653 24.44024596105521 90-91 22.355425249948187 26.655797424898083 26.62695373576675 24.361823589386983 92-93 22.474747474747474 26.29575160327693 26.790204087599168 24.439296834376428 94-95 22.05569778985318 26.000339620669838 27.182428549035997 24.761534040440985 96-97 22.443237592785206 25.705392089291013 27.03443443318003 24.816935884743753 98-99 22.25487129708581 25.95320359220467 26.77496818782057 25.01695692288895 100-101 22.120129295469177 25.794691492677657 27.010791504007457 25.074387707845702 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 160.0 1 116.0 2 105.0 3 152.5 4 142.0 5 138.5 6 232.5 7 476.5 8 775.0 9 932.5 10 911.5 11 836.5 12 864.5 13 965.5 14 1061.5 15 1185.0 16 1351.0 17 1533.5 18 1699.0 19 1934.5 20 2329.5 21 2849.0 22 3520.0 23 4397.0 24 5511.0 25 6983.0 26 9029.0 27 11583.0 28 13953.0 29 16308.0 30 18942.5 31 22440.5 32 27891.5 33 30994.0 34 32166.0 35 34868.0 36 39213.5 37 43482.5 38 46322.5 39 49084.0 40 51718.0 41 54933.5 42 57102.5 43 58713.0 44 62040.0 45 66161.0 46 69078.0 47 71361.0 48 72902.5 49 73062.5 50 73125.0 51 73164.0 52 73929.0 53 75273.0 54 75223.5 55 71491.5 56 66084.0 57 60801.5 58 55191.0 59 48201.5 60 39675.0 61 32478.0 62 26249.5 63 19525.0 64 13813.5 65 9910.0 66 7258.5 67 5581.5 68 4125.5 69 2952.5 70 2155.5 71 1463.0 72 1001.0 73 765.5 74 553.0 75 394.0 76 267.5 77 167.0 78 97.0 79 52.0 80 40.5 81 27.5 82 18.0 83 11.5 84 2.5 85 3.0 86 3.5 87 2.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022291690159080182 2 0.02391005642355673 3 0.0 4 0.0 5 0.0 6 1.0441072674042241E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.654375435914784E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.83080450553168E-5 26-27 3.132321802212672E-4 28-29 2.6102681685105602E-5 30-31 4.4374558864679526E-4 32-33 0.002166522579863765 34-35 0.0042808397963573185 36-37 0.10503719110086494 38-39 0.09668433296163115 40-41 0.10587247691478832 42-43 0.1062118117766947 44-45 0.10164384248180121 46-47 0.09381303797626953 48-49 0.09472663183524822 50-51 0.09420457820154611 52-53 0.07499300448130838 54-55 0.06442141839884062 56-57 0.03489928541298619 58-59 0.013808318611420862 60-61 0.009631889541803966 62-63 0.0021143172164935537 64-65 0.005768692652408338 66-67 0.16674393060445458 68-69 0.4365673511833912 70-71 0.41819106327707684 72-73 0.19616165286356857 74-75 0.03150593679392246 76-77 0.03393348619063728 78-79 0.005011714883540275 80-81 0.0016705716278467586 82-83 7.83080450553168E-5 84-85 3.9154022527658407E-4 86-87 0.0012529287208850688 88-89 2.871294985361616E-4 90-91 7.830804505531681E-4 92-93 0.0031323218022126726 94-95 0.08415504575278045 96-97 0.017541002092390963 98-99 0.01905495763012709 100-101 0.010910920944374142 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1915512.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.3602139856661 #Duplication Level Percentage of deduplicated Percentage of total 1 77.42038156617488 45.18270035053885 2 12.965198730992844 15.133035446148579 3 4.24905843446354 7.439278784186748 4 1.821307617819559 4.251676092386929 5 0.941684898371554 2.7478466088017064 6 0.5800638089665523 2.0311588809977117 7 0.3852676419756669 1.573901141921704 8 0.27446934279145707 1.281447166225166 9 0.19110215228720268 1.003748625054225 >10 1.0723927422107038 11.393992402117364 >50 0.06781374905354112 2.666259100280702 >100 0.027409013209150228 2.873320983158711 >500 0.002507173189306129 1.015611767492264 >1k 0.0013431284942711388 1.406022650689315 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3832 0.20005095243464935 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3354 0.17509678874368836 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2201 0.11490400477783486 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1958 0.10221810147887354 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 5.2205363370211205E-5 0.0 0.0 0.0 5.2205363370211205E-5 8 5.2205363370211205E-5 0.0 0.0 5.2205363370211205E-5 5.2205363370211205E-5 9 5.2205363370211205E-5 0.0 0.0 1.0441072674042241E-4 5.2205363370211205E-5 10-11 1.30513408425528E-4 0.0 0.0 1.0441072674042241E-4 5.2205363370211205E-5 12-13 1.566160901106336E-4 0.0 0.0 1.30513408425528E-4 1.0441072674042241E-4 14-15 2.0882145348084482E-4 0.0 0.0 1.566160901106336E-4 1.5661609011063363E-4 16-17 2.0882145348084482E-4 0.0 0.0 2.0882145348084482E-4 2.0882145348084482E-4 18-19 2.871294985361616E-4 0.0 0.0 2.61026816851056E-4 2.0882145348084482E-4 20-21 3.654375435914784E-4 0.0 0.0 2.871294985361616E-4 2.3492413516595042E-4 22-23 3.91540225276584E-4 0.0 0.0 4.959509520170065E-4 2.61026816851056E-4 24-25 6.786697238127457E-4 0.0 0.0 7.047724054978512E-4 2.61026816851056E-4 26-27 6.786697238127457E-4 0.0 0.0 0.0015139555377361247 3.132321802212672E-4 28-29 9.657992223489072E-4 0.0 0.0 0.004594071976578586 3.132321802212672E-4 30-31 0.0010963126307744352 0.0 0.0 0.01989024344405047 3.654375435914784E-4 32-33 0.0011224153124595408 0.0 0.0 0.05646010048488342 5.481563153872176E-4 34-35 0.0011485179941446465 0.0 0.0 0.11286799560639663 5.742589970723233E-4 36-37 0.0011485179941446465 0.0 0.0 0.21318060132225744 5.742589970723233E-4 38-39 0.0011485179941446465 0.0 0.0 0.37068418261018465 6.003616787574288E-4 40-41 0.0011485179941446465 0.0 0.0 0.5367755461725116 7.047724054978512E-4 42-43 0.0012268260391999632 0.0 0.0 0.6987165833469067 7.83080450553168E-4 44-45 0.0016183662644765472 0.0 0.0 0.9023175004907305 8.091831322382737E-4 46-47 0.0016966743095318641 0.0 0.0 1.1443676677567147 8.352858139233793E-4 48-49 0.0017749823545871808 0.0 0.0 1.4000173321806388 8.352858139233793E-4 50-51 0.0017749823545871808 0.0 0.0 1.6792638208478987 8.352858139233793E-4 52-53 0.0017749823545871808 0.0 0.0 1.9332429136439762 9.396965406638017E-4 54-55 0.001827187717957392 0.0 0.0 2.205311164847832 9.91901904034013E-4 56-57 0.0019315984446978145 0.0 0.0 2.5218583856431076 9.91901904034013E-4 58-59 0.0019315984446978145 0.0 0.0 2.841642338967336 9.91901904034013E-4 60-61 0.0019315984446978145 0.0 0.0 3.1618178325168413 0.001044107267404224 62-63 0.0019315984446978145 0.0 0.0 3.4680544940465 0.0011485179941446465 64-65 0.0019315984446978145 0.0 0.0 3.786324491832993 0.001174620675829752 66-67 0.0019315984446978145 0.0 0.0 4.133751185061748 0.0012790314025701744 68-69 0.001983803808068026 0.0 0.0 4.522524526079711 0.00130513408425528 70-71 0.001983803808068026 0.0 0.0 4.9685671507147955 0.0013834421293105969 72-73 0.0020621118531233425 0.0 0.0 5.409337033649489 0.0015922635827914416 74-75 0.0021404198981786592 0.0 0.0 5.83966584391014 0.0016183662644765472 76-77 0.0021404198981786592 0.0 0.0 6.313690543311658 0.0016183662644765472 78-79 0.0021404198981786592 0.0 0.0 6.845167245102093 0.0016183662644765472 80-81 0.0025058574417701376 0.0 0.0 7.403634119754927 0.001644468946161653 82-83 0.00261026816851056 0.0 0.0 7.976849009559846 0.0016705716278467586 84-85 0.002688576213565877 0.0 0.0 8.515503948813684 0.0016705716278467586 86-87 0.0027668842586211937 0.0 0.0 9.072221943793618 0.0017749823545871808 88-89 0.002871294985361616 0.0 0.0 9.665588103859438 0.0018793930813276033 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2325 0.0 58.577286 1 GTATCAA 4160 0.0 40.153152 1 ATCAACG 5350 0.0 30.411692 3 TCAACGC 5385 0.0 30.214031 4 CAACGCA 5425 0.0 29.991257 5 TATCAAC 5490 0.0 29.815199 2 AACGCAG 5725 0.0 28.41966 6 TGGTATC 1015 0.0 25.709143 2 ACGCAGA 6700 0.0 24.354763 7 CGCAGAG 6810 0.0 23.961369 8 TACATGG 5675 0.0 23.074598 2 GTACATG 5735 0.0 23.002853 1 GTGGTAT 1310 0.0 22.096775 1 ACATGGG 5640 0.0 20.942112 3 TAAGGTG 1095 0.0 20.79349 5 GCAGAGT 7835 0.0 20.402864 9 GAGTACA 4045 0.0 20.295412 1 AGAGTAC 6985 0.0 19.9995 10-11 GAGTACT 4870 0.0 19.33445 12-13 CAGAGTA 7495 0.0 19.08165 10-11 >>END_MODULE