FastQCFastQC Report
Fri 27 May 2016
SRR939781_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939781_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305026
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89330.387544435507452No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67150.291319924373955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45510.19743812000385247No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69550.034.5863381
GTATCAA110000.033.7293431
TATCAAC132150.028.3278142
ATCAACG133350.028.0735053
TACATGG109450.027.8739452
GTACATG108100.027.8703121
TCAACGC134900.027.6466274
CAACGCA137100.027.1343615
AACGCAG141750.026.443946
ACATGGG113300.025.461643
CATGGGA71350.023.3423564
ACGCAGA158700.023.230917
CGCAGAG158750.023.19328
GAGTACT81450.022.74722712-13
GCAGAGT160800.021.9827949
GTACTTT88500.021.01831814-15
AGTACAT79250.020.9542452
AGTACTT85050.020.36184312-13
GAGTACA93250.019.2834261
CATGGGG44950.019.212034
Produced by FastQC (version 0.11.5)