##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939781_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2305026 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.925301493345412 31.0 30.0 33.0 16.0 34.0 2 29.736560889117953 31.0 30.0 33.0 25.0 34.0 3 29.99540786090916 31.0 30.0 34.0 25.0 34.0 4 33.76998220410529 35.0 35.0 37.0 28.0 37.0 5 33.737323570319816 35.0 35.0 37.0 28.0 37.0 6 33.76736488004907 35.0 35.0 37.0 28.0 37.0 7 33.68372591025004 35.0 33.0 37.0 28.0 37.0 8 33.55043891045047 35.0 33.0 37.0 28.0 37.0 9 34.82266317169525 37.0 34.0 39.0 27.0 39.0 10-11 34.66231508885366 37.0 34.0 39.0 27.0 39.0 12-13 34.547610092033665 37.0 34.0 39.0 27.0 39.0 14-15 35.214333807948364 38.0 33.5 40.0 26.5 41.0 16-17 35.055944271344444 38.0 33.0 40.0 26.5 41.0 18-19 34.91974732606053 38.0 33.5 40.0 25.5 41.0 20-21 35.11486356336111 38.0 34.0 40.0 26.0 41.0 22-23 35.015926067645225 38.0 34.0 40.0 25.5 41.0 24-25 34.808266154047715 38.0 33.5 40.0 24.5 41.0 26-27 34.69045425084142 38.0 33.0 40.0 24.5 41.0 28-29 34.74379703309204 38.0 33.5 40.0 24.5 41.0 30-31 34.51619569584031 38.0 33.0 40.0 24.0 41.0 32-33 34.16593717381062 38.0 33.0 40.0 23.5 41.0 34-35 33.98455028273 38.0 33.0 40.0 22.5 41.0 36-37 33.78632800671229 38.0 33.0 40.0 21.0 41.0 38-39 33.568489466062424 38.0 32.0 40.0 20.0 41.0 40-41 33.33128281416349 37.5 32.0 40.0 18.5 41.0 42-43 32.93055978544277 37.0 31.0 40.0 16.0 41.0 44-45 32.58177716867402 37.0 31.0 40.0 14.5 41.0 46-47 32.18162853694492 37.0 30.0 40.0 11.0 41.0 48-49 31.815840905916026 36.5 30.0 39.0 9.0 41.0 50-51 31.189575301970564 35.5 29.5 38.5 8.0 40.0 52-53 31.331434873185813 36.0 30.0 39.0 7.5 40.0 54-55 31.4347239467147 36.0 30.0 39.0 6.5 40.0 56-57 31.138910146783594 36.0 29.0 39.0 2.0 40.0 58-59 30.743284023694308 35.5 28.5 39.0 2.0 40.0 60-61 30.333191035589188 35.0 28.0 39.0 2.0 40.0 62-63 29.88279546521384 35.0 28.0 38.0 2.0 40.0 64-65 29.338400738212933 34.0 26.5 38.0 2.0 40.0 66-67 28.74451611391802 34.0 26.0 38.0 2.0 40.0 68-69 28.19296658692787 34.0 25.5 37.0 2.0 39.0 70-71 27.62324416297257 33.0 24.5 36.0 2.0 39.0 72-73 26.96876933275373 32.5 22.5 36.0 2.0 38.5 74-75 26.61261781862764 32.0 22.0 35.5 2.0 38.0 76-77 26.030529807472888 32.0 20.5 35.0 2.0 37.5 78-79 25.35495890284969 31.0 19.0 35.0 2.0 37.0 80-81 24.626216363719976 31.0 15.0 34.5 2.0 36.0 82-83 23.880947980630154 30.5 7.5 34.0 2.0 36.0 84-85 23.085293831826625 30.0 3.5 34.0 2.0 35.0 86-87 22.11463796937648 29.0 2.0 34.0 2.0 35.0 88-89 21.084122044610343 28.5 2.0 34.0 2.0 35.0 90-91 19.67817629822831 26.0 2.0 33.0 2.0 34.5 92-93 18.103543300596176 22.0 2.0 32.0 2.0 34.0 94-95 17.615136662232878 21.0 2.0 32.0 2.0 34.0 96-97 17.335607928066757 21.5 2.0 32.0 2.0 34.0 98-99 16.460908683893372 10.0 2.0 32.0 2.0 34.0 100-101 14.840743661893619 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.5072915442950006 1101 2 1.0036525718246594 1101 3 0.938292141638982 1101 4 1.032700509759863 1101 5 0.9863611008542037 1101 6 0.9672911435184162 1101 7 0.7980774458926589 1101 8 1.0312571763145542 1101 9 1.1073273075069707 1101 10-11 1.0064964187060355 1101 12-13 1.0647347517690235 1101 14-15 1.2745419769793287 1101 16-17 1.1341116023071294 1101 18-19 1.0736504214109672 1101 20-21 1.0072259030375577 1101 22-23 0.9855834565452852 1101 24-25 1.2572629950706826 1101 26-27 1.0175918557024346 1101 28-29 1.0674039279448877 1101 30-31 1.2269911762227608 1101 32-33 1.512122636186696 1101 34-35 1.2804218939775822 1101 36-37 1.1180556806792126 1101 38-39 1.0963148472217412 1101 40-41 1.161042668933355 1101 42-43 1.0971280318352399 1101 44-45 1.1701539842476265 1101 46-47 1.0097069447805112 1101 48-49 1.0726457246454473 1101 50-51 1.0108877502636382 1101 52-53 0.8047940514038423 1101 54-55 0.9279090995800807 1101 56-57 0.9751492180626222 1101 58-59 1.0354691111341943 1101 60-61 0.8819982383820069 1101 62-63 0.6349219751938371 1101 64-65 0.6964464020086929 1101 66-67 0.7511844609816265 1101 68-69 0.6341344799720368 1101 70-71 0.5596091471900522 1101 72-73 0.5729462184213183 1101 74-75 0.5782199373625225 1101 76-77 0.4142213574372242 1101 78-79 0.2570265831644285 1101 80-81 0.15259678640953567 1101 82-83 -0.10736861516394569 1101 84-85 -0.1533116150343652 1101 86-87 -0.4818688998914844 1101 88-89 -4.225923827043175 1101 90-91 -7.678017190672218 1101 92-93 -10.316635768677434 1101 94-95 -8.364345265662951 1101 96-97 -4.944735920233057 1101 98-99 -3.37099656889189 1101 100-101 -2.3422536432144927 1102 1 1.9543792701400733 1102 2 1.227499012723996 1102 3 1.0911067698692278 1102 4 1.2154255773967648 1102 5 1.076443509039045 1102 6 1.113172289147677 1102 7 1.065106086624077 1102 8 1.1813697490047943 1102 9 1.536373238408487 1102 10-11 1.482521856402336 1102 12-13 1.4816491094516522 1102 14-15 1.6802576842903392 1102 16-17 1.673137475367433 1102 18-19 1.5312380384150401 1102 20-21 1.5201083236613684 1102 22-23 1.4685133007471691 1102 24-25 1.6789240642002667 1102 26-27 1.5111024443767533 1102 28-29 1.5148062042766384 1102 30-31 1.641841753080051 1102 32-33 1.8397043446793973 1102 34-35 1.9268937352563071 1102 36-37 1.7675809784370884 1102 38-39 1.7565110719939483 1102 40-41 1.7036365054230842 1102 42-43 1.6041904132762994 1102 44-45 1.7775158339226564 1102 46-47 1.6720020904719632 1102 48-49 1.6162699744199926 1102 50-51 1.7035647723739054 1102 52-53 1.8116903725780809 1102 54-55 1.818439155244473 1102 56-57 1.9853452873476947 1102 58-59 1.8038627734783184 1102 60-61 1.7067855319728267 1102 62-63 1.7597161102188714 1102 64-65 1.5602577028141162 1102 66-67 1.4559622698568297 1102 68-69 1.4438852340846715 1102 70-71 1.3555666370056443 1102 72-73 1.3818298207474058 1102 74-75 1.192130438964039 1102 76-77 1.3341582296578913 1102 78-79 1.1404535197567753 1102 80-81 1.0803893354657284 1102 82-83 0.9324814952319223 1102 84-85 0.9712579965587551 1102 86-87 0.9475234563606527 1102 88-89 0.5707893302787141 1102 90-91 -5.347124538994512 1102 92-93 -10.350239338662197 1102 94-95 -8.173778048571805 1102 96-97 -4.473870012343639 1102 98-99 -2.8505565402822555 1102 100-101 -1.962858021732389 1103 1 2.0594321568021563 1103 2 1.3506388103465525 1103 3 1.007766559632465 1103 4 0.8298690394264341 1103 5 0.8940752344868628 1103 6 0.72249540536734 1103 7 0.79356860750039 1103 8 0.8815295551416114 1103 9 1.0305350748691708 1103 10-11 0.9235589589336968 1103 12-13 0.9686340785055876 1103 14-15 1.0049916136219679 1103 16-17 0.9368493658609296 1103 18-19 0.8673198408186735 1103 20-21 0.6797454851376088 1103 22-23 0.7418063550747007 1103 24-25 0.7392629972978213 1103 26-27 0.9508028078702182 1103 28-29 0.5432687251529487 1103 30-31 0.722805829446429 1103 32-33 0.7970767126264775 1103 34-35 0.8617917278554472 1103 36-37 0.8064990122976496 1103 38-39 0.6412122541663976 1103 40-41 0.5794743414908012 1103 42-43 0.7411249517050891 1103 44-45 0.763501359678294 1103 46-47 0.7396724575676217 1103 48-49 0.6675209159008233 1103 50-51 0.6677675427835759 1103 52-53 0.41961714993014354 1103 54-55 0.3867530040180931 1103 56-57 0.5394435943195681 1103 58-59 0.41649630001989024 1103 60-61 0.44324589153663396 1103 62-63 0.47068706348867195 1103 64-65 0.43400004816565385 1103 66-67 0.23636342255683473 1103 68-69 0.4343728392849222 1103 70-71 0.282501830377619 1103 72-73 0.4247508673466136 1103 74-75 0.21216940808347218 1103 76-77 0.2834227284626216 1103 78-79 0.18416246300594707 1103 80-81 -0.27764845475597255 1103 82-83 -0.4882657949406557 1103 84-85 -0.6478453862227873 1103 86-87 -0.5980530573767773 1103 88-89 -1.5236282614951975 1103 90-91 -6.807459214961373 1103 92-93 -10.245286658307217 1103 94-95 -8.845532641360881 1103 96-97 -5.397271459768074 1103 98-99 -4.0117672027572855 1103 100-101 -3.124836932561875 1104 1 1.951384133268668 1104 2 1.2814235416249495 1104 3 1.1719233944663934 1104 4 0.9801646671079993 1104 5 0.7622191489452774 1104 6 0.7676816635027421 1104 7 0.9096407026803348 1104 8 0.9612542827741706 1104 9 1.0034446189760828 1104 10-11 1.0474321875078374 1104 12-13 0.8627206911061691 1104 14-15 1.1132459947897217 1104 16-17 1.0225174397009553 1104 18-19 0.9503916741196079 1104 20-21 0.9389901745077864 1104 22-23 0.9841171171653826 1104 24-25 0.9798203827219609 1104 26-27 0.975102698381292 1104 28-29 0.8508681813844774 1104 30-31 1.0738643171500541 1104 32-33 1.1107331159853544 1104 34-35 1.1472157918463992 1104 36-37 0.969127019405903 1104 38-39 1.031883638445244 1104 40-41 0.9633023836173251 1104 42-43 1.0644057879594442 1104 44-45 0.8529057573508751 1104 46-47 0.7951429624483808 1104 48-49 0.7676683223321277 1104 50-51 0.8821693349304631 1104 52-53 0.7851127133357529 1104 54-55 0.9077529725622888 1104 56-57 0.8833968608529084 1104 58-59 0.505073845959572 1104 60-61 0.5360820981489454 1104 62-63 0.6207977462197789 1104 64-65 0.6588319964962821 1104 66-67 0.7552877819670272 1104 68-69 0.5282178106961446 1104 70-71 0.45170883721414157 1104 72-73 0.29297702167028916 1104 74-75 0.4392436244455631 1104 76-77 0.5955170481134395 1104 78-79 0.40206475522427354 1104 80-81 0.142876569091694 1104 82-83 -0.17063080123370966 1104 84-85 -0.10974426302330187 1104 86-87 0.17367967973648035 1104 88-89 -0.27164540907558177 1104 90-91 -3.054907978956834 1104 92-93 -7.913093503013718 1104 94-95 -5.537880221898154 1104 96-97 -3.0905170465760428 1104 98-99 -2.517212940932083 1104 100-101 -1.8712121252286558 1105 1 1.8969549732335764 1105 2 1.1394587394410038 1105 3 0.8545084239504916 1105 4 0.704709353670836 1105 5 0.6761850452207128 1105 6 0.6208935143446297 1105 7 0.6156300969539075 1105 8 0.5814288077693561 1105 9 0.7349543025558916 1105 10-11 0.6350999567150666 1105 12-13 0.5765652612610026 1105 14-15 0.6004177531559094 1105 16-17 0.6622043033015714 1105 18-19 0.6027674642810368 1105 20-21 0.4780546695365189 1105 22-23 0.6427688007308205 1105 24-25 0.5549827088996935 1105 26-27 0.38021721669541364 1105 28-29 0.5626676667982977 1105 30-31 0.7879642509824833 1105 32-33 0.8874266595214522 1105 34-35 0.8826423759240427 1105 36-37 0.8381682971473836 1105 38-39 0.6572251164296787 1105 40-41 0.5658055529642994 1105 42-43 0.7633851655454791 1105 44-45 0.5797987493770975 1105 46-47 0.6482701649601097 1105 48-49 0.4972393227032512 1105 50-51 0.6040659001565878 1105 52-53 0.300718082253038 1105 54-55 0.3623970731506141 1105 56-57 0.2929166863430446 1105 58-59 0.48773994868235704 1105 60-61 0.42273041772353537 1105 62-63 0.3953407492739309 1105 64-65 0.4543537877192598 1105 66-67 0.3211943097814256 1105 68-69 0.32635698503538535 1105 70-71 0.21393489346451844 1105 72-73 0.1494272622607049 1105 74-75 0.056115162139700914 1105 76-77 0.1146166433525515 1105 78-79 -0.31871246588170266 1105 80-81 -0.2627382728722374 1105 82-83 -0.4211240314480378 1105 84-85 -0.36753162566128594 1105 86-87 -0.21416440390325775 1105 88-89 -0.4033225544804182 1105 90-91 -3.3435201019596548 1105 92-93 -8.12764107991507 1105 94-95 -5.802178885419245 1105 96-97 -3.1750636095890172 1105 98-99 -2.1338867136447917 1105 100-101 -1.4471469738206952 1106 1 1.540125132450708 1106 2 1.0180020047990403 1106 3 0.7994781106423154 1106 4 0.6926275828957174 1106 5 0.7944358536398326 1106 6 0.7619673724405729 1106 7 0.8069045663264802 1106 8 -0.17488880940805984 1106 9 -0.34348335763442606 1106 10-11 -0.44479767564790507 1106 12-13 -0.28747752810294 1106 14-15 0.4771092665858774 1106 16-17 0.8676703342816552 1106 18-19 0.8935950387924194 1106 20-21 0.8800862200439283 1106 22-23 0.6302632603137184 1106 24-25 0.9597634625505833 1106 26-27 0.8708339662916842 1106 28-29 0.6861534467155863 1106 30-31 0.8108158217630006 1106 32-33 0.8301032880186625 1106 34-35 0.6704087495427942 1106 36-37 0.6088301023790805 1106 38-39 0.6787651018607974 1106 40-41 0.48217551022118954 1106 42-43 0.5920382756947546 1106 44-45 0.4880031917316998 1106 46-47 0.5779592849298893 1106 48-49 0.40943941751665136 1106 50-51 0.4507651605349885 1106 52-53 0.3495398523344022 1106 54-55 0.2307120540530363 1106 56-57 0.3879545924315302 1106 58-59 0.24578309263299047 1106 60-61 0.13978892838233747 1106 62-63 0.34682538834706733 1106 64-65 0.4428869550649104 1106 66-67 0.41980776017975785 1106 68-69 0.11653551172767251 1106 70-71 0.2977381826415275 1106 72-73 0.31855740714020087 1106 74-75 0.16289759622319266 1106 76-77 -0.008761604295418834 1106 78-79 -0.0890262365643224 1106 80-81 -0.13703478443291672 1106 82-83 -0.3600986977703933 1106 84-85 -0.26669391459548564 1106 86-87 -0.12480335370996798 1106 88-89 -0.550576241989333 1106 90-91 -4.086279210405371 1106 92-93 -8.941970429287029 1106 94-95 -6.2978569746594655 1106 96-97 -3.655279791846578 1106 98-99 -2.606334749350129 1106 100-101 -1.9403312008795144 1107 1 1.8789969385582026 1107 2 1.0999141094816522 1107 3 0.8388493047916938 1107 4 0.7893638062830917 1107 5 0.5864456449696718 1107 6 0.7255777742175198 1107 7 0.675225912332138 1107 8 0.5989371376160122 1107 9 0.7838545490155155 1107 10-11 0.6832470048495196 1107 12-13 0.7232408049533774 1107 14-15 0.7235017357235449 1107 16-17 0.7137432252926672 1107 18-19 0.7585628921037895 1107 20-21 0.6669243986752562 1107 22-23 0.6995318192656867 1107 24-25 0.5389000349807347 1107 26-27 0.587335303533493 1107 28-29 0.6143003091081098 1107 30-31 0.699281733429487 1107 32-33 0.8812731857234368 1107 34-35 0.8172824746300549 1107 36-37 0.5213703329588526 1107 38-39 0.5734416328893346 1107 40-41 0.44704854779994463 1107 42-43 0.635273116510092 1107 44-45 0.5253411361568467 1107 46-47 0.4788723113271942 1107 48-49 0.5103080580630319 1107 50-51 0.587491146845263 1107 52-53 0.4880357952787513 1107 54-55 0.3607444651680076 1107 56-57 0.5958343086642834 1107 58-59 0.5005042361636107 1107 60-61 0.29614347122696216 1107 62-63 0.30559867675311025 1107 64-65 0.3787533668749461 1107 66-67 0.48704892456228777 1107 68-69 0.24641908858507833 1107 70-71 0.15775767464074164 1107 72-73 0.17019156195805252 1107 74-75 0.09939409824038847 1107 76-77 0.12384990807217733 1107 78-79 -0.13220101482394142 1107 80-81 0.042672004524636264 1107 82-83 -0.1806142158193822 1107 84-85 0.031820387092313496 1107 86-87 0.17763730146296552 1107 88-89 0.08738348210066249 1107 90-91 -2.8670070628101385 1107 92-93 -4.367665115072979 1107 94-95 -2.602671422915007 1107 96-97 -1.0221471707015368 1107 98-99 -0.9260341343699992 1107 100-101 -0.6431898385549797 1108 1 1.5005593836960713 1108 2 0.8345590842521524 1108 3 -0.5854713958578728 1108 4 -0.31081798203828015 1108 5 0.2551095291235086 1108 6 0.5066771688227689 1108 7 0.46603413414786843 1108 8 0.4927049412619695 1108 9 0.691228868725112 1108 10-11 0.47593682073929244 1108 12-13 0.48849035331416246 1108 14-15 0.32781127989358083 1108 16-17 0.3469435209110614 1108 18-19 0.4149288104746063 1108 20-21 0.16535180227364066 1108 22-23 0.06379123006765042 1108 24-25 0.09754835952669083 1108 26-27 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32-33 0.7966305592547727 2312 34-35 0.6479076992650334 2312 36-37 0.5359089409978353 2312 38-39 0.36241248637626455 2312 40-41 0.49296650745782244 2312 42-43 0.6177779474055427 2312 44-45 0.28475184980310786 2312 46-47 0.36224763178432795 2312 48-49 0.5268293392019174 2312 50-51 0.4742806703339575 2312 52-53 0.3383048930650929 2312 54-55 0.5285106551332781 2312 56-57 0.5850452811961517 2312 58-59 0.7489535087773085 2312 60-61 0.6321728664134376 2312 62-63 0.470499485903229 2312 64-65 0.33814781728018417 2312 66-67 0.6682526795970922 2312 68-69 0.5722530397336847 2312 70-71 0.7531975348000799 2312 72-73 0.5221539598859053 2312 74-75 0.7744762599351311 2312 76-77 0.8463239996184733 2312 78-79 1.175292940754364 2312 80-81 1.2353264240811086 2312 82-83 1.1780529974867022 2312 84-85 1.311551945803771 2312 86-87 1.9345184262890598 2312 88-89 2.426220151415425 2312 90-91 3.2174308225507566 2312 92-93 4.17570352767418 2312 94-95 3.941276960442572 2312 96-97 3.042645846814356 2312 98-99 2.910969894802374 2312 100-101 2.5496523294262285 2313 1 -7.293815998920337 2313 2 -2.6676041820738554 2313 3 -1.50075356374683 2313 4 -0.7028639334881603 2313 5 -0.4159078244827654 2313 6 -0.17925066816077617 2313 7 0.015829863836401614 2313 8 0.014914184025059285 2313 9 0.006546643365609839 2313 10-11 -0.1877024391400255 2313 12-13 -0.2505646310063696 2313 14-15 -0.2691156075765875 2313 16-17 -0.4745785740152897 2313 18-19 -0.5849984143226905 2313 20-21 -0.4414603315077912 2313 22-23 -1.2280445166121723 2313 24-25 -0.1963600749006602 2313 26-27 -0.3044460430419633 2313 28-29 -0.5094064052794849 2313 30-31 -0.00769646197979057 2313 32-33 -0.2620447835837254 2313 34-35 -0.1768886603708708 2313 36-37 -0.10876715086269684 2313 38-39 -0.10410432712017581 2313 40-41 -0.20091725953781037 2313 42-43 -0.028998258856397285 2313 44-45 -0.09982890267144029 2313 46-47 -1.0990774496279343 2313 48-49 -0.8265817831900364 2313 50-51 -0.7260832344115045 2313 52-53 -1.1664756815167259 2313 54-55 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-2.0158449539487826 2314 76-77 -1.0766427647380965 2314 78-79 -2.087639852341148 2314 80-81 -1.0351923440522448 2314 82-83 -0.5042201438360401 2314 84-85 -1.5009343095129566 2314 86-87 -0.27127231324285006 2314 88-89 0.6214015579785439 2314 90-91 1.6599053350205715 2314 92-93 2.7664530306126522 2314 94-95 2.6212718819047467 2314 96-97 1.9233220830509588 2314 98-99 1.5668681426486835 2314 100-101 1.5265702005741044 2315 1 -2.949008613867967 2315 2 -2.1084527529952126 2315 3 -2.876145598778489 2315 4 -4.165728600036481 2315 5 -4.018236364633072 2315 6 -3.0512549287679143 2315 7 -5.64658211320366 2315 8 -3.0464740638002574 2315 9 -2.574499572386806 2315 10-11 -4.204357270786865 2315 12-13 -3.098040813028973 2315 14-15 -2.1258290225715584 2315 16-17 -2.5615940778912503 2315 18-19 -2.080089839850821 2315 20-21 -1.5187992273004411 2315 22-23 -1.0623867853854776 2315 24-25 -2.5863343337325446 2315 26-27 -3.9944617244432266 2315 28-29 -3.271881995551098 2315 30-31 -3.4573902308418525 2315 32-33 -4.065414805478511 2315 34-35 -3.702478931984576 2315 36-37 -2.92571933738261 2315 38-39 -1.9495167775610298 2315 40-41 -1.4387005603248397 2315 42-43 -2.965518171277253 2315 44-45 -6.192399468977143 2315 46-47 -2.989338707690539 2315 48-49 -2.000877599510531 2315 50-51 -3.2228951759465083 2315 52-53 -3.026239572871244 2315 54-55 -1.6042926851230241 2315 56-57 -1.2040962860086033 2315 58-59 -2.595294562151885 2315 60-61 -2.8091963801718762 2315 62-63 -4.671720869396406 2315 64-65 -4.102854556732254 2315 66-67 -3.5228304375792874 2315 68-69 -2.0364535543161324 2315 70-71 -3.5102803339963664 2315 72-73 -2.353629553355379 2315 74-75 -1.5712430896032856 2315 76-77 -3.2818523933682364 2315 78-79 -1.6115390154140599 2315 80-81 -1.2099063866129391 2315 82-83 -0.9811389469156531 2315 84-85 -2.4582395630163205 2315 86-87 -1.9960610971851374 2315 88-89 -1.248933609715543 2315 90-91 -0.32858735840306963 2315 92-93 0.14783272565017924 2315 94-95 -0.13194772988101988 2315 96-97 -0.3262142558119159 2315 98-99 -0.6337491902966121 2315 100-101 -0.8330489490938593 2316 1 -6.973135515743262 2316 2 -5.996471050308688 2316 3 -3.401807104435349 2316 4 -4.1073803018593615 2316 5 -1.993175686286385 2316 6 -2.797326992308193 2316 7 -3.200779714659344 2316 8 -2.4334577766896786 2316 9 -6.638216005721031 2316 10-11 -6.716567987699673 2316 12-13 -5.875385081866064 2316 14-15 -4.830116859801713 2316 16-17 -4.940012688498577 2316 18-19 -4.049622981395913 2316 20-21 -2.9028710496163725 2316 22-23 -3.4656381768925897 2316 24-25 -3.304675201604148 2316 26-27 -4.160118226928432 2316 28-29 -4.123636902679724 2316 30-31 -6.705997160234595 2316 32-33 -4.782932309134104 2316 34-35 -5.604360705251558 2316 36-37 -4.152534178338332 2316 38-39 -6.4723360796781435 2316 40-41 -3.834178646135186 2316 42-43 -4.382280657615318 2316 44-45 -3.1077634497149305 2316 46-47 -4.212315624605132 2316 48-49 -5.357268630134108 2316 50-51 -4.715457723037371 2316 52-53 -6.192755399868716 2316 54-55 -5.205109646470543 2316 56-57 -4.390379778222421 2316 58-59 -3.055085069229289 2316 60-61 -3.5390274188958273 2316 62-63 -4.386549689451979 2316 64-65 -3.0900148696687246 2316 66-67 -4.2896800327095335 2316 68-69 -3.2051295304557677 2316 70-71 -2.6868204472229067 2316 72-73 -2.108499381550036 2316 74-75 -2.475887849525499 2316 76-77 -3.0379828421348627 2316 78-79 -4.739336845586159 2316 80-81 -5.532960882468945 2316 82-83 -3.1020918128693644 2316 84-85 -1.846162264014275 2316 86-87 -1.9511489207161254 2316 88-89 -1.0235378231008134 2316 90-91 -0.9335602281091582 2316 92-93 -0.7407779859020849 2316 94-95 -1.3995369307732552 2316 96-97 -3.2794421204999455 2316 98-99 -4.620581914612918 2316 100-101 -4.106291287242746 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18412.0 3 6261.0 4 6029.0 5 6622.0 6 7449.0 7 8698.0 8 10608.0 9 12598.0 10 15011.0 11 17624.0 12 20681.0 13 23460.0 14 25723.0 15 28801.0 16 32042.0 17 34868.0 18 32011.0 19 31442.0 20 33541.0 21 36596.0 22 40578.0 23 45534.0 24 50853.0 25 57938.0 26 64867.0 27 74786.0 28 84325.0 29 96255.0 30 108587.0 31 124114.0 32 138224.0 33 154233.0 34 176613.0 35 206267.0 36 226830.0 37 186981.0 38 58436.0 39 1128.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.790390546939484 19.398714105738737 13.108519049480863 22.702376297840914 2 15.746188364570479 20.195619285153025 40.27274460772114 23.78544774255536 3 19.600650899243544 24.99606871452612 29.399846918784423 26.003433467445912 4 12.688119816264813 16.189045834309024 37.27727669453916 33.845557654886996 5 13.4740512897143 37.77652481496235 34.10670001594197 14.64272387938138 6 31.695142863225197 36.09202053918176 17.706290048384986 14.506546549208057 7 28.577432041893918 32.958996979639736 20.666130679472413 17.797440298993937 8 26.484229773499347 35.01635118681893 19.803620638560993 18.69579840112073 9 27.257216958091497 16.22218513863756 19.991021716494835 36.52957618677611 10-11 25.078131654009745 26.7001891151047 27.33173883343096 20.88994039745459 12-13 26.14018900516474 24.104947638934483 27.67796603173231 22.076897324168474 14-15 22.97772103847979 25.219565440321478 26.261013034009395 25.541700487189335 16-17 22.120114213239326 28.64023198939641 26.783539059246326 22.45611473811794 18-19 22.0668595132366 27.13013580017022 29.299757489607863 21.503247196985317 20-21 23.004944369863377 26.228563134044702 29.325606551235744 21.440885944856173 22-23 22.513113132696425 26.33603964915713 29.13602225917593 22.014824958970515 24-25 22.185267757167637 26.415187872035943 29.469703788836433 21.92984058195999 26-27 22.012318220999077 26.66470379993024 29.370372077771272 21.952605901299407 28-29 21.880879092587744 27.004534104064344 29.333230990553442 21.78135581279447 30-31 22.09343054457268 26.720646981050074 29.25982061304133 21.92610186133592 32-33 21.853118876764295 26.688958542242663 29.24076878053193 22.217153800461116 34-35 22.23726409597735 26.74683330753484 29.14939761969776 21.86650497679005 36-37 22.316963348098916 26.494042527841472 29.14382508804344 22.045169036016173 38-39 22.049451052319107 26.745229275847326 29.174999124996763 22.030320546836805 40-41 22.229166942945774 26.619863355433488 29.23142377185875 21.919545929761988 42-43 21.971542444382898 26.625796665039168 29.074083816183688 22.328577074394243 44-45 22.03033161757034 26.84580739401106 28.946566445240496 22.177294543178103 46-47 22.177615981720766 26.98189360085483 28.484886695052236 22.35560372237217 48-49 22.112925981127024 26.97877752580849 28.46000752935568 22.44828896370881 50-51 22.115418075272167 27.250665952141922 28.37662964709321 22.2572863254927 52-53 22.123503854873704 27.287977596319646 28.169287331202465 22.419231217604185 54-55 21.8371093078842 27.257939986037194 28.327138516680332 22.577812189398273 56-57 21.953194460359494 27.40092509987343 28.219286025373385 22.426594414393687 58-59 22.020177465503508 27.031232911025242 28.19120126129272 22.757388362178528 60-61 21.87126868226568 27.07401217150003 28.4626900288865 22.59202911734779 62-63 21.925790168301457 27.01374024298425 28.45881752533042 22.60165206338387 64-65 21.76312288307729 27.017710042530275 28.383995298130316 22.835171776262122 66-67 21.79727712554607 27.20446382554057 28.066418294746125 22.93184075416723 68-69 21.782369980771062 27.085348575045682 28.256996520705563 22.875284923477697 70-71 21.872190251596937 27.071279132254045 27.97381162316285 23.082718992986173 72-73 21.883376769946867 27.16840032574742 27.814937193592478 23.133285710713242 74-75 21.843149995607273 27.36533240781575 27.726840966678758 23.06467662989822 76-77 22.048819997790243 27.11927996680143 27.75197551518252 23.079924520225813 78-79 22.03827864830725 27.20393008389793 27.745451903971492 23.012339363823322 80-81 22.005617139235188 27.405218388474424 27.521894096565898 23.06727037572449 82-83 22.122220668194643 27.394840488252747 27.44154107139012 23.041397772162494 84-85 22.043095398498043 27.218518391264013 27.453440677501696 23.284945532736245 86-87 21.964743831605812 27.145457736287028 27.707131239750517 23.18266719235665 88-89 22.153983731209166 27.042841497160225 27.504734407770602 23.298440363860006 90-91 21.948411297046245 27.18181008154127 27.62885030274339 23.240928318669095 92-93 22.23098554183169 26.917601596509567 27.63081218082517 23.220600680833574 94-95 22.07597616918728 26.833127720166427 27.65062506692948 23.440271043716812 96-97 22.490241171368357 26.810166716449764 27.094833210670394 23.604758901511484 98-99 22.517275155309374 26.803763854453678 26.879342998962173 23.799617991274776 100-101 22.71274287149604 26.869848782252454 26.88948179723492 23.527926549016588 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3378.0 1 2059.0 2 774.5 3 806.5 4 715.0 5 687.5 6 989.5 7 1754.0 8 2639.0 9 2997.0 10 2797.0 11 2478.5 12 2437.0 13 2606.5 14 2766.5 15 2896.5 16 3108.0 17 3308.0 18 3561.5 19 3999.5 20 4485.5 21 5190.5 22 6131.0 23 7320.5 24 8888.5 25 10932.0 26 13375.0 27 16428.0 28 19642.5 29 22904.5 30 26580.5 31 30409.5 32 34660.0 33 38524.0 34 41981.5 35 45902.5 36 50511.0 37 54783.0 38 58461.5 39 61615.5 40 64973.5 41 69249.0 42 73514.0 43 77524.5 44 81042.5 45 84500.0 46 88286.5 47 91584.0 48 94963.5 49 94734.0 50 90769.0 51 87420.5 52 83835.5 53 80620.0 54 77457.0 55 71857.0 56 65260.0 57 58936.5 58 52598.0 59 45855.5 60 38170.5 61 31155.0 62 24911.5 63 18935.0 64 13919.0 65 10294.5 66 7618.5 67 5718.0 68 4234.5 69 3079.5 70 2253.5 71 1573.5 72 1186.0 73 939.5 74 679.5 75 501.0 76 339.5 77 219.0 78 147.0 79 99.0 80 78.5 81 52.0 82 31.0 83 23.5 84 19.5 85 13.0 86 11.0 87 11.0 88 9.5 89 7.5 90 4.5 91 3.0 92 1.5 93 2.5 94 2.0 95 0.5 96 1.0 97 1.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13561669152538844 2 0.12143030056927775 3 0.1292827065725072 4 0.14034548851075865 5 0.12698338326769418 6 0.1422543606883393 7 0.17249263131955994 8 0.15895699224216994 9 0.16997639072184 10-11 0.16407624035477256 12-13 0.154206503527509 14-15 0.15887022532500716 16-17 0.1536425185659511 18-19 0.16865319523510797 20-21 0.1746184207900475 22-23 0.2104531575782659 24-25 0.2320581199517923 26-27 0.24589744323925197 28-29 0.2413855635467886 30-31 0.23255702972547815 32-33 0.23357654100214054 34-35 0.22342481169409803 36-37 0.23032278160853717 38-39 0.21841402222794884 40-41 0.22201484929020324 42-43 0.21557240569086855 44-45 0.20763323277047632 46-47 0.22335973650622595 48-49 0.2613636462235133 50-51 0.26299052592031497 52-53 0.2654199996008722 54-55 0.2629471424617336 56-57 0.23819687934105732 58-59 0.23776304475524357 60-61 0.20895642825720837 62-63 0.20086541323178134 64-65 0.20236214255283888 66-67 0.21025793201464973 68-69 0.2102796237439404 70-71 0.21683052598972852 72-73 0.247220638725984 74-75 0.2504093229317153 76-77 0.26602736802101146 78-79 0.26212285674868746 80-81 0.2753548116160078 82-83 0.2750294356766475 84-85 0.2496718041358319 86-87 0.25273033796581906 88-89 0.25596240563013173 90-91 0.4308194354423768 92-93 0.5759804878556685 94-95 0.3397792476093545 96-97 0.2916453003133153 98-99 0.3225343228232566 100-101 0.3416447363283538 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2305026.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.878786531422236 #Duplication Level Percentage of deduplicated Percentage of total 1 77.35715023728118 46.32052285737318 2 12.397869348670907 14.847387443470561 3 4.474618499568429 8.038041778356323 4 2.0162013093311626 4.829107512232587 5 1.1173377152962525 3.3452413268865664 6 0.6622283877290126 2.3792059358324074 7 0.4233145664795962 1.7743293793311299 8 0.2914927614528409 1.3963386270791553 9 0.20202606705497103 1.0887368168670664 >10 0.987545422990627 10.220693627550585 >50 0.04845151243346043 1.9956930003978444 >100 0.020069656541683526 2.171428461441974 >500 0.0011051185891052067 0.42783153769496274 >1k 4.4204743564182556E-4 0.47512328943829274 >5k 1.4734914521394185E-4 0.6903184060473913 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8933 0.387544435507452 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6715 0.291319924373955 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4551 0.19743812000385247 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.1691729290689128E-5 0.0 12-13 0.0 0.0 0.0 4.3383458581378255E-5 0.0 14-15 0.0 0.0 0.0 4.3383458581378255E-5 0.0 16-17 0.0 0.0 0.0 8.676691716275651E-5 0.0 18-19 0.0 0.0 0.0 1.7353383432551305E-4 0.0 20-21 0.0 0.0 0.0 3.036842100696478E-4 0.0 22-23 0.0 0.0 0.0 8.025939837554977E-4 0.0 24-25 0.0 2.1691729290689128E-5 0.0 0.00149672932105755 0.0 26-27 4.3383458581378255E-5 4.3383458581378255E-5 0.0 0.003318834581475437 0.0 28-29 4.3383458581378255E-5 4.3383458581378255E-5 0.0 0.00921898494854288 0.0 30-31 4.3383458581378255E-5 4.3383458581378255E-5 0.0 0.02995627815044169 0.0 32-33 4.3383458581378255E-5 4.3383458581378255E-5 0.0 0.07505338334578439 0.0 34-35 4.3383458581378255E-5 4.3383458581378255E-5 0.0 0.14592026293846577 0.0 36-37 4.3383458581378255E-5 4.3383458581378255E-5 0.0 0.26665642817044144 0.0 38-39 4.3383458581378255E-5 4.3383458581378255E-5 0.0 0.4442249241440227 0.0 40-41 4.3383458581378255E-5 4.3383458581378255E-5 0.0 0.643832217076944 0.0 42-43 4.3383458581378255E-5 4.3383458581378255E-5 0.0 0.8377779686649955 0.0 44-45 4.3383458581378255E-5 4.3383458581378255E-5 0.0 1.0667992465160911 0.0 46-47 4.3383458581378255E-5 4.3383458581378255E-5 0.0 1.3359285318256715 0.0 48-49 4.3383458581378255E-5 4.3383458581378255E-5 0.0 1.630697441156846 0.0 50-51 4.3383458581378255E-5 4.3383458581378255E-5 0.0 1.943427102340711 0.0 52-53 4.3383458581378255E-5 6.507518787206739E-5 0.0 2.2405170267059895 0.0 54-55 4.3383458581378255E-5 8.676691716275651E-5 0.0 2.555480936006796 0.0 56-57 4.3383458581378255E-5 8.676691716275651E-5 0.0 2.9090561234450285 0.0 58-59 4.3383458581378255E-5 8.676691716275651E-5 0.0 3.300201386014735 0.0 60-61 4.3383458581378255E-5 8.676691716275651E-5 0.0 3.727658603417055 4.3383458581378255E-5 62-63 4.3383458581378255E-5 8.676691716275651E-5 0.0 4.134335144158895 4.3383458581378255E-5 64-65 4.3383458581378255E-5 8.676691716275651E-5 0.0 4.5285172488292975 4.3383458581378255E-5 66-67 4.3383458581378255E-5 8.676691716275651E-5 0.0 4.968924428618159 4.3383458581378255E-5 68-69 4.3383458581378255E-5 8.676691716275651E-5 0.0 5.4624980368984986 4.3383458581378255E-5 70-71 4.3383458581378255E-5 8.676691716275651E-5 0.0 5.988392321821967 4.3383458581378255E-5 72-73 4.3383458581378255E-5 8.676691716275651E-5 0.0 6.5002520578943574 4.3383458581378255E-5 74-75 4.3383458581378255E-5 8.676691716275651E-5 0.0 6.9913528090355594 4.3383458581378255E-5 76-77 4.3383458581378255E-5 8.676691716275651E-5 0.0 7.504947883451206 4.3383458581378255E-5 78-79 4.3383458581378255E-5 8.676691716275651E-5 0.0 8.07099355929174 4.3383458581378255E-5 80-81 4.3383458581378255E-5 8.676691716275651E-5 0.0 8.661225513291392 6.507518787206739E-5 82-83 4.3383458581378255E-5 8.676691716275651E-5 0.0 9.211848369606244 8.676691716275651E-5 84-85 4.3383458581378255E-5 1.0845864645344565E-4 0.0 9.719543293654823 8.676691716275651E-5 86-87 4.3383458581378255E-5 1.3015037574413477E-4 0.0 10.228995247775947 8.676691716275651E-5 88-89 4.3383458581378255E-5 1.7353383432551302E-4 0.0 10.778923968753498 8.676691716275651E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6955 0.0 34.586338 1 GTATCAA 11000 0.0 33.729343 1 TATCAAC 13215 0.0 28.327814 2 ATCAACG 13335 0.0 28.073505 3 TACATGG 10945 0.0 27.873945 2 GTACATG 10810 0.0 27.870312 1 TCAACGC 13490 0.0 27.646627 4 CAACGCA 13710 0.0 27.134361 5 AACGCAG 14175 0.0 26.44394 6 ACATGGG 11330 0.0 25.46164 3 CATGGGA 7135 0.0 23.342356 4 ACGCAGA 15870 0.0 23.23091 7 CGCAGAG 15875 0.0 23.1932 8 GAGTACT 8145 0.0 22.747227 12-13 GCAGAGT 16080 0.0 21.982794 9 GTACTTT 8850 0.0 21.018318 14-15 AGTACAT 7925 0.0 20.954245 2 AGTACTT 8505 0.0 20.361843 12-13 GAGTACA 9325 0.0 19.283426 1 CATGGGG 4495 0.0 19.21203 4 >>END_MODULE