FastQCFastQC Report
Fri 27 May 2016
SRR939781_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939781_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305026
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98900.429062405369831No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81110.3518832325535591No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55830.2422098492598348No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72500.037.4195561
GTATCAA108600.031.62791
GTACATG111250.026.0821781
TACATGG110800.025.7124462
ATCAACG133550.025.5689333
TCAACGC134900.025.3114074
TATCAAC135650.025.2442822
CAACGCA137500.024.832795
ACATGGG112100.024.444913
AACGCAG141200.024.1663346
CATGGGA73550.021.9938414
ACGCAGA157800.021.5643887
CGCAGAG158550.021.4326538
GAGTACT86200.020.6952312-13
ATGGGAG28700.020.540825
GTACTTT90250.019.97563614-15
GCAGAGT169250.019.4928939
AGTACTT88650.019.43212512-13
GTGTAGC14400.018.6758841
CATGGGG41550.018.6149754
Produced by FastQC (version 0.11.5)