##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939781_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2305026 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.475023275225528 31.0 31.0 34.0 30.0 34.0 2 31.6864885688925 31.0 31.0 34.0 30.0 34.0 3 31.746261430456748 31.0 31.0 34.0 30.0 34.0 4 35.5403153803905 37.0 35.0 37.0 33.0 37.0 5 35.27819512664933 37.0 35.0 37.0 33.0 37.0 6 35.308156610814805 37.0 35.0 37.0 32.0 37.0 7 35.22975359063195 37.0 35.0 37.0 32.0 37.0 8 35.17212387192162 37.0 35.0 37.0 32.0 37.0 9 36.78637334242651 39.0 37.0 39.0 32.0 39.0 10-11 36.64723630015453 39.0 35.0 39.0 32.0 39.0 12-13 36.58771484573276 39.0 35.0 39.0 32.0 39.0 14-15 37.64028236557853 39.0 36.0 41.0 32.0 41.0 16-17 37.568778616813866 39.0 36.0 41.0 32.0 41.0 18-19 37.56122577359214 39.0 36.0 41.0 32.0 41.0 20-21 37.49593215000611 39.0 36.0 41.0 32.0 41.0 22-23 37.35118432503582 39.0 36.0 41.0 31.5 41.0 24-25 37.192874397078384 39.0 36.0 40.5 31.0 41.0 26-27 36.90309241631114 39.0 36.0 40.0 31.0 41.0 28-29 36.68477644069958 38.5 35.5 40.0 30.0 41.0 30-31 36.42420888094104 38.0 35.0 40.0 30.0 41.0 32-33 36.37963845093287 38.0 35.0 40.0 30.0 41.0 34-35 36.45697380420004 38.0 35.0 40.0 30.0 41.0 36-37 36.42204599861346 38.0 35.0 40.0 30.0 41.0 38-39 36.28848763528047 38.0 35.0 40.0 30.0 41.0 40-41 36.12050124380376 38.0 35.0 40.0 30.0 41.0 42-43 35.91670896553878 38.0 35.0 40.0 29.0 41.0 44-45 35.65652101104283 38.0 34.5 40.0 28.0 41.0 46-47 35.33928098858755 38.0 34.0 40.0 27.0 41.0 48-49 35.01805771388262 38.0 34.0 40.0 26.0 41.0 50-51 34.752612768793064 38.0 33.5 40.0 25.5 41.0 52-53 34.47830892146119 38.0 33.0 40.0 24.0 41.0 54-55 34.17933789033182 38.0 33.0 40.0 24.0 41.0 56-57 32.795641133765955 36.5 31.5 39.5 16.0 41.0 58-59 29.317948691251203 34.5 23.5 39.0 7.0 40.0 60-61 29.316186021329045 34.0 24.0 38.0 9.0 40.0 62-63 31.323917821317416 35.0 29.5 39.0 9.0 40.0 64-65 30.92080089335218 35.0 29.5 38.5 4.5 40.0 66-67 25.43697446362861 28.5 18.0 36.0 2.0 39.0 68-69 17.717832900800254 13.0 7.0 29.5 2.0 35.5 70-71 16.567883832980627 15.0 7.5 26.0 2.0 33.5 72-73 18.72908873912919 19.5 7.5 30.0 2.0 35.0 74-75 19.421672250117787 19.0 7.5 31.0 2.0 35.5 76-77 20.344279196850707 23.0 8.5 31.5 2.0 34.5 78-79 23.402531034357096 28.5 12.0 34.0 2.0 36.0 80-81 26.083871288219743 31.5 22.0 35.0 2.0 36.0 82-83 27.149267730602602 33.0 26.0 35.0 2.0 36.0 84-85 27.165251715165034 33.0 26.0 35.0 2.0 36.0 86-87 26.88384838175361 33.0 26.0 35.0 2.0 36.0 88-89 26.44942204556478 33.0 25.0 35.0 2.0 35.0 90-91 24.750944891727904 32.0 12.5 34.5 2.0 35.0 92-93 22.570284456661227 31.0 2.0 34.0 2.0 35.0 94-95 18.164628728699803 23.0 2.0 33.0 2.0 35.0 96-97 16.866049884036016 15.0 2.0 32.5 2.0 35.0 98-99 15.78569005295385 4.5 2.0 32.0 2.0 34.0 100-101 15.165772316668011 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.38178410420210795 1101 2 0.43650524207998487 1101 3 0.32032187067872187 1101 4 0.35414433924504607 1101 5 0.3914333593033845 1101 6 0.40928615713215066 1101 7 0.31881604860033974 1101 8 0.3590736522959972 1101 9 0.4457931751966484 1101 10-11 0.48774921292432083 1101 12-13 0.39329081752864425 1101 14-15 0.4424624586840409 1101 16-17 0.4232737212022428 1101 18-19 0.47084119642794775 1101 20-21 0.573547410055852 1101 22-23 0.6599865573047623 1101 24-25 0.48000783146394355 1101 26-27 0.6157475541124597 1101 28-29 0.5957095663434515 1101 30-31 0.5268870227531508 1101 32-33 0.5156653497150927 1101 34-35 0.6201337931426849 1101 36-37 0.5341363001097719 1101 38-39 0.73081343224802 1101 40-41 0.8479707838179209 1101 42-43 0.7796798839643486 1101 44-45 0.7330442032684346 1101 46-47 0.685802611099291 1101 48-49 0.587674141082509 1101 50-51 0.7237634467499845 1101 52-53 0.7215296349697979 1101 54-55 0.5443369189708491 1101 56-57 1.4238879057452465 1101 58-59 -8.1560202314513 1101 60-61 -9.475178219410523 1101 62-63 -2.187649452938796 1101 64-65 0.41593835014244007 1101 66-67 -0.3814780797167998 1101 68-69 -3.1379797335979767 1101 70-71 -3.782116946836366 1101 72-73 -6.134213493954885 1101 74-75 -7.207104702678176 1101 76-77 -8.502106757678062 1101 78-79 -11.702284077370429 1101 80-81 -7.343811937583304 1101 82-83 -2.9009252090212954 1101 84-85 -1.8144828323925317 1101 86-87 -1.6191661672615787 1101 88-89 -2.100732563107485 1101 90-91 -8.264459374028906 1101 92-93 -13.98590828630724 1101 94-95 -12.445232419082807 1101 96-97 -12.625318864928083 1101 98-99 -12.28938414111757 1101 100-101 -12.107652752288868 1102 1 0.49993660599021084 1102 2 0.49911276143813765 1102 3 0.4681966724326152 1102 4 0.3098967415213778 1102 5 0.5044420354858588 1102 6 0.5318557376278434 1102 7 0.4874602007289539 1102 8 0.6220157799820427 1102 9 0.5524091530552795 1102 10-11 0.6478771788415472 1102 12-13 0.6596570830384891 1102 14-15 0.695763761666278 1102 16-17 0.9047878549833754 1102 18-19 0.8334732827544045 1102 20-21 0.9848322003036998 1102 22-23 0.8686125174378532 1102 24-25 0.8291779965912838 1102 26-27 0.9289538544514215 1102 28-29 0.8591542284698974 1102 30-31 0.7752189567022114 1102 32-33 0.7672710654224062 1102 34-35 0.8143347447239933 1102 36-37 0.8869672544898393 1102 38-39 0.7237278832229208 1102 40-41 0.7046643497600158 1102 42-43 0.8753752224598585 1102 44-45 0.8275152360140297 1102 46-47 0.8312168288434805 1102 48-49 0.8152381240411231 1102 50-51 0.8234582254917839 1102 52-53 0.7036083425606776 1102 54-55 0.6536240104283806 1102 56-57 1.1680953877864795 1102 58-59 -8.508264688334716 1102 60-61 -9.67725630505284 1102 62-63 -2.7710297433887767 1102 64-65 -0.05059926219702149 1102 66-67 -1.143035127948913 1102 68-69 -3.8163116675470405 1102 70-71 -4.371489245247286 1102 72-73 -6.841994536962627 1102 74-75 -7.563096710639119 1102 76-77 -8.72484348685056 1102 78-79 -11.857083032496838 1102 80-81 -7.917824594236599 1102 82-83 -3.724274713017312 1102 84-85 -2.70644399170466 1102 86-87 -2.504963349834579 1102 88-89 -2.7298770728565316 1102 90-91 -8.585040584962762 1102 92-93 -14.411276168883349 1102 94-95 -12.727432406643835 1102 96-97 -12.473149974795296 1102 98-99 -11.950402115431093 1102 100-101 -11.72800912881653 1103 1 0.279650395818944 1103 2 0.20724322612032253 1103 3 0.20312937702049183 1103 4 0.17689153472306884 1103 5 0.1646306591227571 1103 6 0.18476794249406936 1103 7 0.15380136063333083 1103 8 0.23722421565024376 1103 9 0.3191609623358218 1103 10-11 0.17391084292118109 1103 12-13 0.21881024150199124 1103 14-15 0.4450904800734534 1103 16-17 0.4434114918828769 1103 18-19 0.35413477536992133 1103 20-21 0.1715005043283213 1103 22-23 0.308404900448636 1103 24-25 0.34778689726915246 1103 26-27 0.28115282619062043 1103 28-29 0.49594107721076597 1103 30-31 0.4009513469173598 1103 32-33 0.3052852776894923 1103 34-35 0.41157022954568134 1103 36-37 0.2959298244868904 1103 38-39 0.3240029593600724 1103 40-41 0.3147973032262996 1103 42-43 0.2065259576335805 1103 44-45 0.1849511334499212 1103 46-47 0.09063622171115071 1103 48-49 -0.002987307402698036 1103 50-51 0.12362163381610713 1103 52-53 0.14615246935768056 1103 54-55 0.18103272772215462 1103 56-57 0.713427935187994 1103 58-59 -6.786983946939031 1103 60-61 -8.892508294442763 1103 62-63 -2.6465198046259637 1103 64-65 -0.32501390672661046 1103 66-67 -1.876301657077505 1103 68-69 -4.305368549362541 1103 70-71 -4.939583033111191 1103 72-73 -8.014012416911541 1103 74-75 -8.276169049113756 1103 76-77 -9.183362005369077 1103 78-79 -12.44912154315604 1103 80-81 -8.722900403910113 1103 82-83 -4.878114317431631 1103 84-85 -3.980358587113507 1103 86-87 -3.9015632122965123 1103 88-89 -3.9435473744578644 1103 90-91 -9.551028403019547 1103 92-93 -15.14274520644434 1103 94-95 -12.75989204577014 1103 96-97 -12.620036374900089 1103 98-99 -12.209450810456799 1103 100-101 -11.94378671011135 1104 1 0.17903086813348068 1104 2 0.2002484792390895 1104 3 0.17808411667068214 1104 4 0.17010788279553424 1104 5 -0.01624758684113914 1104 6 0.06710638189977658 1104 7 0.12008623725269274 1104 8 0.13395917930363765 1104 9 0.10382231789893837 1104 10-11 0.2637744763390728 1104 12-13 0.24127953703985128 1104 14-15 0.21872079785662635 1104 16-17 0.4153226932936178 1104 18-19 0.31257749340883834 1104 20-21 0.1792470638027197 1104 22-23 0.1921363012588273 1104 24-25 0.4030009236761032 1104 26-27 0.380845923688824 1104 28-29 0.4543728046686155 1104 30-31 0.4979383928121379 1104 32-33 0.45092089899651455 1104 34-35 0.4441952009796424 1104 36-37 0.42120738403215086 1104 38-39 0.5024788158958415 1104 40-41 0.39879007142395295 1104 42-43 0.3314266160451851 1104 44-45 0.26244022529820654 1104 46-47 0.18906985546632882 1104 48-49 0.11003632272558406 1104 50-51 0.09802845510130709 1104 52-53 0.16431108845559805 1104 54-55 0.12470675769915118 1104 56-57 0.6501525070574914 1104 58-59 -6.4472136387138725 1104 60-61 -8.708160719969257 1104 62-63 -2.444101346210342 1104 64-65 -0.26413082490703843 1104 66-67 -1.869586222549728 1104 68-69 -4.332455878624813 1104 70-71 -4.997570854789391 1104 72-73 -7.859693573332901 1104 74-75 -8.715720947964027 1104 76-77 -9.136569606454948 1104 78-79 -12.325514078201781 1104 80-81 -8.607573394329737 1104 82-83 -4.85773399026856 1104 84-85 -3.8353307638890435 1104 86-87 -3.5624074291474983 1104 88-89 -3.5730657823547105 1104 90-91 -9.313758338292603 1104 92-93 -15.199595029249645 1104 94-95 -12.80416760161878 1104 96-97 -12.489828971265691 1104 98-99 -11.791997369779597 1104 100-101 -11.359315188412344 1105 1 0.07482224773669444 1105 2 0.12265367216351208 1105 3 0.061067778313429244 1105 4 0.0873168977764891 1105 5 0.022592471888721377 1105 6 0.015281363901365808 1105 7 0.10851693854944955 1105 8 0.10538757778749641 1105 9 0.10445278244518619 1105 10-11 0.07551885994600838 1105 12-13 0.0752034855751802 1105 14-15 0.13350972180705867 1105 16-17 0.16066703513440217 1105 18-19 0.022139993006874192 1105 20-21 0.06410017672378387 1105 22-23 0.050082121787717426 1105 24-25 0.1104185575405836 1105 26-27 0.006914147783881219 1105 28-29 0.05819686628276344 1105 30-31 0.17432555668259653 1105 32-33 -0.017517379980922954 1105 34-35 0.19919597842017112 1105 36-37 0.1184755412408478 1105 38-39 0.18358699211115947 1105 40-41 0.2600866417195178 1105 42-43 0.1327101684679448 1105 44-45 0.08848610980042793 1105 46-47 0.19856408784121982 1105 48-49 0.17894422496404871 1105 50-51 0.2830771765994058 1105 52-53 0.23055668248636607 1105 54-55 0.23327074748612375 1105 56-57 1.0013554969689196 1105 58-59 -6.3788829529659274 1105 60-61 -8.390716146182584 1105 62-63 -2.237031290684822 1105 64-65 0.23527512351555302 1105 66-67 -1.423107044525679 1105 68-69 -3.9787104122758805 1105 70-71 -4.437930573306739 1105 72-73 -5.70292193390944 1105 74-75 -6.38657267851638 1105 76-77 -8.377610313695055 1105 78-79 -11.786758100327246 1105 80-81 -7.826898069005868 1105 82-83 -3.7515452609998334 1105 84-85 -2.8321331377497927 1105 86-87 -2.638718702716144 1105 88-89 -2.6386182058695056 1105 90-91 -8.104888252577421 1105 92-93 -11.379493727424531 1105 94-95 -11.706262018839144 1105 96-97 -12.119945655442109 1105 98-99 -11.685240030722081 1105 100-101 -11.331155479050258 1106 1 0.09887131945185246 1106 2 0.16237007989196073 1106 3 0.0623516133997839 1106 4 0.12543612369831436 1106 5 0.12707182684927432 1106 6 0.11821942300171884 1106 7 0.030962276312543224 1106 8 0.15329725068126976 1106 9 0.1516058752505458 1106 10-11 0.17382628661015787 1106 12-13 0.18523366398624574 1106 14-15 0.18557345491120003 1106 16-17 0.11507412378492887 1106 18-19 0.22264951867008875 1106 20-21 0.21185683775046016 1106 22-23 0.19464249209956108 1106 24-25 0.21257440610123268 1106 26-27 0.24732142143616187 1106 28-29 0.2291294292893511 1106 30-31 0.2207162880947422 1106 32-33 0.16541813534531968 1106 34-35 0.21476657393311172 1106 36-37 0.06611330009876326 1106 38-39 0.19134877922809324 1106 40-41 0.2775394373658102 1106 42-43 0.03693379704608901 1106 44-45 0.13030999583934744 1106 46-47 0.09825710055623915 1106 48-49 0.1731253416808869 1106 50-51 0.06763203540067053 1106 52-53 0.21463265653986952 1106 54-55 -0.04796313712674305 1106 56-57 0.8475357888862689 1106 58-59 -6.997989201785575 1106 60-61 -9.29188512601354 1106 62-63 -2.6725867006371438 1106 64-65 -0.06789399506671856 1106 66-67 -1.5836933844586945 1106 68-69 -4.079188911603323 1106 70-71 -4.510451453452582 1106 72-73 -6.120555106265554 1106 74-75 -6.729977944128795 1106 76-77 -8.649373650201682 1106 78-79 -11.97361546012097 1106 80-81 -7.995219602053734 1106 82-83 -4.181547342269564 1106 84-85 -3.1215585531365413 1106 86-87 -2.8523319411333006 1106 88-89 -2.9892738058340456 1106 90-91 -8.268419203756228 1106 92-93 -11.558598935609318 1106 94-95 -11.772477422547658 1106 96-97 -12.126394893658848 1106 98-99 -11.658563741394756 1106 100-101 -11.41821614806285 1107 1 9.92396498133985E-4 1107 2 0.01649966391154223 1107 3 0.027569349608061344 1107 4 0.0011988048303663845 1107 5 -0.051299986813255316 1107 6 -0.01190478183048782 1107 7 -0.03183067030355602 1107 8 0.03619793214573974 1107 9 -0.21613713256136435 1107 10-11 -0.021246128433439537 1107 12-13 0.032970552217015836 1107 14-15 0.027367738769100924 1107 16-17 0.07966290304181456 1107 18-19 -0.033625696270753735 1107 20-21 -0.06070722897564451 1107 22-23 -0.08054090645893552 1107 24-25 -0.019733960348354174 1107 26-27 0.15482905202500064 1107 28-29 0.10835250690981724 1107 30-31 0.24321972563723904 1107 32-33 0.19017486310706033 1107 34-35 0.019809562101919198 1107 36-37 -0.03166211882811609 1107 38-39 0.0458748138906131 1107 40-41 0.09326638316596814 1107 42-43 0.19329835031665965 1107 44-45 0.12964326369568369 1107 46-47 -0.04612553947639242 1107 48-49 -0.07783131506349861 1107 50-51 -0.14040161474060397 1107 52-53 0.03899387803848953 1107 54-55 0.08269445342039461 1107 56-57 0.8124972204149614 1107 58-59 -6.413731068786678 1107 60-61 -8.68651236041616 1107 62-63 -2.5097282140624486 1107 64-65 -0.27984228838353076 1107 66-67 -1.853275099754626 1107 68-69 -4.140860879388638 1107 70-71 -4.6596064224013745 1107 72-73 -6.095267637052336 1107 74-75 -6.781979084939602 1107 76-77 -9.170962928091107 1107 78-79 -12.52170176888028 1107 80-81 -8.55020743844182 1107 82-83 -4.248326893802481 1107 84-85 -3.2446380623612434 1107 86-87 -2.9328907495526373 1107 88-89 -3.0430851338587104 1107 90-91 -8.304415184475772 1107 92-93 -11.502348085667492 1107 94-95 -11.752184211505213 1107 96-97 -12.523661102993852 1107 98-99 -11.839632326033852 1107 100-101 -11.315313691164343 1108 1 -0.023498766097642232 1108 2 -0.018385571592112626 1108 3 -0.004308025391232206 1108 4 -0.0425903773487164 1108 5 -0.29181910290293445 1108 6 -0.09574544238468263 1108 7 0.025953664186957326 1108 8 -0.014515912648946028 1108 9 0.040905041196559466 1108 10-11 -0.06719333903039626 1108 12-13 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5.863102152688345 2315 80-81 2.9517634046149652 2315 82-83 1.2017445232732413 2315 84-85 0.7984670045629265 2315 86-87 0.7012080541275232 2315 88-89 0.8340979486766322 2315 90-91 2.184266047250844 2315 92-93 4.229527735796527 2315 94-95 8.366867047849418 2315 96-97 9.078836818199246 2315 98-99 9.376692343368791 2315 100-101 8.568394833285106 2316 1 -0.4447962995366517 2316 2 -0.43383013920357527 2316 3 -0.5158342889936591 2316 4 -0.2943780980511903 2316 5 -0.6096451288971139 2316 6 -0.47959012493340225 2316 7 -0.5921210991007726 2316 8 -0.6031485365947518 2316 9 -0.7696114973812982 2316 10-11 -0.5923757107492875 2316 12-13 -0.6417162611085772 2316 14-15 -0.7253687397684416 2316 16-17 -0.8203071208991517 2316 18-19 -0.9712455838904503 2316 20-21 -0.7626502392366206 2316 22-23 -0.9026074898661562 2316 24-25 -2.2310106746000713 2316 26-27 -1.4959670582656273 2316 28-29 -1.1627775406663758 2316 30-31 -0.8465618944524422 2316 32-33 -0.8068017755066279 2316 34-35 -0.9272079197230099 2316 36-37 -0.749497508390597 2316 38-39 -0.9955091794255893 2316 40-41 -0.9139057711356315 2316 42-43 -0.8470087314774375 2316 44-45 -0.8968873763563181 2316 46-47 -0.8085176655433344 2316 48-49 -1.032080022636741 2316 50-51 -0.8939297835955458 2316 52-53 -1.002556528931379 2316 54-55 -0.880950435960635 2316 56-57 0.3257362864646467 2316 58-59 3.8179994039045333 2316 60-61 3.75874062157029 2316 62-63 1.1690998316978956 2316 64-65 0.8689157323723968 2316 66-67 6.062249657412707 2316 68-69 9.090348169393241 2316 70-71 6.1184140477242615 2316 72-73 9.619629799629056 2316 74-75 10.332547157072877 2316 76-77 8.886237175063759 2316 78-79 6.292110348360005 2316 80-81 3.174754244045914 2316 82-83 1.8189342149527903 2316 84-85 1.5471763798514253 2316 86-87 1.4140850416225703 2316 88-89 1.481932662349287 2316 90-91 2.838010310727398 2316 92-93 4.7473813155956215 2316 94-95 8.898938494927474 2316 96-97 9.61561474587364 2316 98-99 9.92455944364367 2316 100-101 8.859452631830019 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 18.0 4 44.0 5 114.0 6 220.0 7 619.0 8 1356.0 9 2340.0 10 3786.0 11 5836.0 12 8260.0 13 11345.0 14 14669.0 15 20612.0 16 27141.0 17 35356.0 18 38695.0 19 38162.0 20 39811.0 21 39585.0 22 38514.0 23 37204.0 24 38083.0 25 41417.0 26 53526.0 27 78683.0 28 111008.0 29 146187.0 30 173986.0 31 185630.0 32 190965.0 33 198088.0 34 203162.0 35 200250.0 36 171321.0 37 110044.0 38 37133.0 39 1849.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.87701048973807 19.46611424936365 14.136499376447686 23.52037588445059 2 15.864273268728828 20.971835605597825 39.20567348808797 23.95821763758538 3 19.418063746948928 24.99290649678124 29.889940067490233 25.699089688779598 4 13.059245318707902 16.909093433219407 36.79260017023669 33.239061077836 5 13.248489768630167 38.0766717821707 34.25823382701203 14.416604622187107 6 31.90944298603528 36.501457903023095 17.252279044504114 14.336820066437514 7 28.344886348353555 32.988478221069954 20.62566756296892 18.040967867607566 8 26.426342134695464 35.44473762072553 19.563761213558916 18.565159031020094 9 26.731542290629264 16.092269675049216 19.93665147377947 37.23953656054205 10-11 25.005422932322674 26.835228756638752 27.23357567333297 20.925772637705606 12-13 25.799470374737638 24.113437332160245 27.846171800231325 22.240920492870796 14-15 22.615684362231683 25.332946930658363 26.27040002221237 25.780968684897587 16-17 21.91302831291274 28.709372475625006 26.90965307983511 22.467946131627148 18-19 21.84418255058015 27.196578749286875 29.324730315118625 21.63450838501435 20-21 22.691869853094932 26.306319321343878 29.41461397832389 21.587196847237298 22-23 22.319921770947488 26.420916727186594 29.173293489964973 22.08586801190095 24-25 22.069707673579387 26.525666087931327 29.408128151265974 21.996498087223312 26-27 21.92102988949356 26.74996008718347 29.311798922701023 22.017211100621946 28-29 21.82495058508303 27.050024902688623 29.26350081476044 21.86152369746791 30-31 21.955398436196692 26.777403708170173 29.255049017725604 22.01214883790753 32-33 21.75860398699101 26.718796332268486 29.204868587993648 22.317731092746858 34-35 22.134789295467904 26.795842091448822 29.124991052080144 21.944377561003122 36-37 22.17274968419399 26.578411670436957 29.19427483085773 22.054563814511326 38-39 21.961197581716025 26.70642029053429 29.247770828885404 22.08461129886428 40-41 22.115353320479556 26.577267387641196 29.2805746966322 22.026804595247054 42-43 21.870334291725822 26.63493578206194 29.100718870307603 22.394011055904635 44-45 21.92399658041026 26.870945204112186 28.95722041165264 22.247837803824908 46-47 22.03157916891876 26.987867001137637 28.543564945295508 22.43698888464809 48-49 21.989112665769515 26.98668462706497 28.526286827965976 22.497915879199542 50-51 21.975269051207874 27.167635701594378 28.477042475169135 22.38005277202861 52-53 22.100756486174117 27.2047980146984 28.226087979079523 22.468357520047956 54-55 21.807427726929156 27.122523823450035 28.427335548553323 22.642712901067487 56-57 21.878304832300262 27.246699403967927 28.311414809298412 22.563580954433395 58-59 20.373786358974225 26.849298964846202 29.9197774377813 22.857137238398277 60-61 21.812383085358622 26.994657879312545 28.531828413363613 22.661130621965224 62-63 21.703536981955875 26.98228558934081 28.52778462275316 22.786392805950154 64-65 20.963282824772364 26.955150483074995 29.018819072774033 23.062747619378605 66-67 21.058627158191506 26.813452644588658 29.095071483393113 23.03284871382672 68-69 21.704603194014805 26.343705539216373 29.1053279096607 22.846363357108125 70-71 20.87676907451316 26.61331196367631 29.28788026195854 23.222038699851982 72-73 20.68949219443632 26.700496223769797 29.197538935058027 23.412472646735853 74-75 21.205910617566254 26.7339350062848 28.6579114032762 23.40224297287275 76-77 19.771572296151245 26.988414152123596 29.391323606644733 23.84868994508043 78-79 20.654737883357118 27.0719453336815 28.468048119636585 23.805268663324792 80-81 21.112791982028806 27.15980585000924 27.912596929660545 23.81480523830141 82-83 21.389534037490705 27.117043658321204 27.55699559809451 23.93642670609358 84-85 21.342904591965556 26.832582365665292 27.565849582607743 24.258663459761408 86-87 21.309477584258328 26.842815400667693 27.6232610907324 24.224445924341584 88-89 21.34251877039972 26.730702111538463 27.542818940614755 24.38396017744706 90-91 21.019904850618516 26.84442956565405 27.674600854105368 24.461064729622066 92-93 20.75032764830214 26.702836895458464 27.866249096142614 24.680586360096783 94-95 19.586838125549814 26.71406716241699 28.566706093863086 25.132388618170115 96-97 19.343225809851546 26.565686641336022 28.826143436078777 25.264944112733655 98-99 19.641760946345393 26.583901887666816 28.31660101272132 25.457736153266463 100-101 19.448555790610598 26.547888260906017 28.472607982265984 25.530947966217397 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 559.0 1 422.0 2 303.0 3 371.5 4 449.0 5 503.0 6 688.0 7 1089.5 8 1556.0 9 1950.0 10 2119.5 11 2208.0 12 2478.0 13 2763.5 14 3019.0 15 3244.0 16 3367.5 17 3514.0 18 3833.5 19 4196.5 20 4665.5 21 5371.5 22 6233.0 23 7403.0 24 8936.5 25 10855.5 26 13276.0 27 16199.0 28 19211.0 29 22483.0 30 26283.5 31 30402.5 32 34979.5 33 39448.5 34 43503.0 35 47927.5 36 52478.0 37 57040.0 38 61324.0 39 64930.5 40 68737.5 41 72904.5 42 77285.0 43 81581.5 44 85595.5 45 89369.5 46 92311.5 47 94681.0 48 96234.0 49 95503.0 50 92869.0 51 89417.5 52 85731.5 53 81683.5 54 76798.5 55 70814.5 56 63770.0 57 56174.0 58 48498.5 59 40795.5 60 33197.0 61 26357.5 62 20244.5 63 14987.5 64 10949.5 65 7931.5 66 5675.0 67 4089.0 68 2906.0 69 1996.5 70 1414.0 71 1007.0 72 723.5 73 523.0 74 356.0 75 240.5 76 158.0 77 89.0 78 49.5 79 27.0 80 17.0 81 12.0 82 5.0 83 2.5 84 3.0 85 2.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02117112778771259 2 0.005639849615579173 3 0.004338345858137826 4 0.0 5 0.06815541343134524 6 1.3015037574413477E-4 7 0.0 8 1.7353383432551302E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.7353383432551302E-4 16-17 0.0 18-19 9.110526302089433E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 8.676691716275651E-5 28-29 0.002342706763394426 30-31 0.008524849611240826 32-33 1.9522556361620217E-4 34-35 9.110526302089433E-4 36-37 0.026246992441733844 38-39 0.025249172894362147 40-41 0.027982330784988973 42-43 0.028047405972861043 44-45 0.028459548829384135 46-47 0.029956278150441686 48-49 0.030238270631220643 50-51 0.02540101499939697 52-53 0.021236202975584657 54-55 0.015791578923621687 56-57 0.1506273681945453 58-59 0.7274104500339692 60-61 0.29826127774697553 62-63 4.3383458581378254E-4 64-65 0.13474902235376085 66-67 1.3631516520854863 68-69 2.6212936426747464 70-71 2.120041162225502 72-73 1.784274884534925 74-75 1.4953410503829458 76-77 1.0790984570239122 78-79 0.3994098114294589 80-81 1.7353383432551302E-4 82-83 6.507518787206739E-5 84-85 0.0 86-87 0.002646390973464074 88-89 2.1691729290689128E-5 90-91 0.2151168793757641 92-93 0.16390270652044706 94-95 1.2441725169260562 96-97 1.2122205996808713 98-99 0.9531128933035896 100-101 0.38496311972186 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2305026.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.264660522323496 #Duplication Level Percentage of deduplicated Percentage of total 1 72.52149016043515 38.62832553968602 2 14.552593376154482 15.50277891800564 3 5.42097940484109 8.662398830921038 4 2.5239093059602555 5.3774068948442455 5 1.4446057313394458 3.8473216934199215 6 0.8548608041940849 2.7320322317543035 7 0.5681494192981338 2.118360016140923 8 0.3838292935345359 1.635562961491225 9 0.27556652221952477 1.321016153160627 >10 1.3495555956592404 12.638308503974214 >50 0.07158518058696821 2.613745373412411 >100 0.030267933977846863 2.8761961746507123 >500 0.0019554538494698974 0.7082647811217178 >1k 4.0738621863940983E-4 0.3147698809726915 >5k 2.444317311836459E-4 1.0235120464442238 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9890 0.429062405369831 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8111 0.3518832325535591 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5583 0.2422098492598348 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.1691729290689128E-5 0.0 12-13 0.0 0.0 0.0 6.507518787206739E-5 0.0 14-15 0.0 0.0 0.0 8.676691716275651E-5 0.0 16-17 0.0 0.0 0.0 1.3015037574413477E-4 0.0 18-19 0.0 0.0 0.0 2.3860902219758042E-4 0.0 20-21 0.0 0.0 0.0 3.904511272324043E-4 0.0 22-23 0.0 0.0 0.0 0.0010845864645344565 0.0 24-25 0.0 0.0 0.0 0.001843796989708576 0.0 26-27 0.0 0.0 0.0 0.0037960526258705974 0.0 28-29 0.0 0.0 0.0 0.010064962390879756 0.0 30-31 0.0 0.0 0.0 0.03206037589163853 0.0 32-33 0.0 0.0 0.0 0.07963033822611978 0.0 34-35 0.0 2.1691729290689128E-5 0.0 0.15390281931743938 0.0 36-37 0.0 4.3383458581378255E-5 0.0 0.27991007476705254 0.0 38-39 0.0 4.3383458581378255E-5 0.0 0.46513575118024697 0.0 40-41 0.0 4.3383458581378255E-5 0.0 0.6727039087628512 0.0 42-43 0.0 4.3383458581378255E-5 0.0 0.8744803746248415 0.0 44-45 0.0 4.3383458581378255E-5 0.0 1.1131978554688753 0.0 46-47 0.0 4.3383458581378255E-5 0.0 1.382435599424909 0.0 48-49 0.0 8.676691716275651E-5 0.0 1.6518902606738493 0.0 50-51 0.0 8.676691716275651E-5 0.0 1.9213883053813712 0.0 52-53 0.0 8.676691716275651E-5 0.0 2.1712987185394006 0.0 54-55 0.0 8.676691716275651E-5 0.0 2.440514770766143 0.0 56-57 0.0 8.676691716275651E-5 0.0 2.676412326802388 0.0 58-59 0.0 8.676691716275651E-5 0.0 2.849707552105703 0.0 60-61 0.0 8.676691716275651E-5 0.0 2.9846951834816613 0.0 62-63 0.0 8.676691716275651E-5 0.0 3.0997698073687676 0.0 64-65 4.3383458581378255E-5 8.676691716275651E-5 0.0 3.212067889906665 0.0 66-67 4.3383458581378255E-5 8.676691716275651E-5 0.0 3.307055972470592 0.0 68-69 4.3383458581378255E-5 8.676691716275651E-5 0.0 3.4162738294492123 0.0 70-71 4.3383458581378255E-5 8.676691716275651E-5 2.1691729290689128E-5 3.612323678778461 0.0 72-73 4.3383458581378255E-5 8.676691716275651E-5 4.3383458581378255E-5 3.8532754077394356 0.0 74-75 4.3383458581378255E-5 8.676691716275651E-5 4.3383458581378255E-5 4.122014241921783 0.0 76-77 4.3383458581378255E-5 8.676691716275651E-5 4.3383458581378255E-5 4.504764805255993 0.0 78-79 4.3383458581378255E-5 8.676691716275651E-5 4.3383458581378255E-5 5.050029804436045 0.0 80-81 4.3383458581378255E-5 8.676691716275651E-5 4.3383458581378255E-5 5.671931683200103 0.0 82-83 4.3383458581378255E-5 8.676691716275651E-5 4.3383458581378255E-5 6.24977765977477 2.1691729290689128E-5 84-85 4.3383458581378255E-5 8.676691716275651E-5 4.3383458581378255E-5 6.704089238038964 4.3383458581378255E-5 86-87 4.3383458581378255E-5 8.676691716275651E-5 4.3383458581378255E-5 7.10688295923777 4.3383458581378255E-5 88-89 4.3383458581378255E-5 8.676691716275651E-5 4.3383458581378255E-5 7.523212319513966 4.3383458581378255E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7250 0.0 37.419556 1 GTATCAA 10860 0.0 31.6279 1 GTACATG 11125 0.0 26.082178 1 TACATGG 11080 0.0 25.712446 2 ATCAACG 13355 0.0 25.568933 3 TCAACGC 13490 0.0 25.311407 4 TATCAAC 13565 0.0 25.244282 2 CAACGCA 13750 0.0 24.83279 5 ACATGGG 11210 0.0 24.44491 3 AACGCAG 14120 0.0 24.166334 6 CATGGGA 7355 0.0 21.993841 4 ACGCAGA 15780 0.0 21.564388 7 CGCAGAG 15855 0.0 21.432653 8 GAGTACT 8620 0.0 20.69523 12-13 ATGGGAG 2870 0.0 20.54082 5 GTACTTT 9025 0.0 19.975636 14-15 GCAGAGT 16925 0.0 19.492893 9 AGTACTT 8865 0.0 19.432125 12-13 GTGTAGC 1440 0.0 18.675884 1 CATGGGG 4155 0.0 18.614975 4 >>END_MODULE