FastQCFastQC Report
Fri 27 May 2016
SRR939780_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939780_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2342537
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91800.39188281764599664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68510.29246069539136416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45130.19265437429590226No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24190.10326411066292657No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66650.036.145531
GTATCAA108050.035.360311
TATCAAC129400.029.3079592
ATCAACG131650.028.736813
TCAACGC134200.028.1557044
GTACATG110750.027.9979691
CAACGCA136600.027.627765
TACATGG110750.027.4456562
AACGCAG141200.026.6616216
ACATGGG114650.025.5692143
CATGGGG45950.023.6553754
ACGCAGA159050.023.5806567
CGCAGAG161250.023.0264558
GCAGAGT162850.021.84669
GAGTACT83000.021.68625812-13
TACCTGG16000.021.1737272
CATGGGA71300.020.7885594
GGACCGA5050.020.5004126
AGTACTT86650.020.14821612-13
GTACTTT90800.019.80166214-15
Produced by FastQC (version 0.11.5)