FastQCFastQC Report
Fri 27 May 2016
SRR939780_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939780_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2342537
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99340.4240701427554826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80550.34385796254232054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56200.2399108317179195No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70400.035.595831
GTATCAA106100.032.7441371
GTACATG113450.026.3974911
TACATGG110500.026.3658582
ATCAACG131400.026.1027373
TATCAAC132900.025.9564482
TCAACGC132800.025.8632814
CAACGCA135100.025.3527435
ACATGGG113400.024.9331023
AACGCAG138550.024.7556826
CATGGGG43850.021.9618824
ACGCAGA158200.021.6208067
CGCAGAG158950.021.4889438
CATGGGA70550.021.3832244
TATCACG1150.00374722820.6304172
GAGTACT84550.020.31131712-13
ATGGGAT27450.020.2202555
GGACCGA4500.020.030156
GCAGAGT170400.019.6552339
AGTACTT88650.019.50571812-13
Produced by FastQC (version 0.11.5)