FastQCFastQC Report
Fri 27 May 2016
SRR939779_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939779_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3500668
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84520.2414396338070334No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT73290.2093600421405286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56990.1627975003627879No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40260.11500662159336446No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36680.10478000198819197No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG112300.026.6148911
TACATGG118300.025.1450732
ACATGGG118600.023.9617463
GAGTACT83850.021.69801112-13
CATGGGG66000.021.6374024
GTATCAA203550.021.4660631
GTACTTT92000.019.8550514-15
TATCAAC229850.019.5880782
AGTACTT91850.019.47241812-13
ATCAACG231050.019.3839513
TCAACGC230100.019.3405484
CAACGCA228700.019.3344695
AGAGTAC149650.019.3065410-11
AACGCAG232100.019.1332826
ATGGGGA41250.017.8274945
GTATATG14200.017.7072621
GAGTACA97650.017.3930071
ACGCAGA254500.017.281477
AGTACAT89500.017.2278232
CATGGGA58300.017.0896534
Produced by FastQC (version 0.11.5)