FastQCFastQC Report
Fri 27 May 2016
SRR939779_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939779_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3500668
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95780.2736049234031905No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT82630.23604066423893955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70090.20021892964428503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50750.14497233099511295No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44190.1262330503778136No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT38080.10877923870529853No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG109350.026.4437271
TACATGG111450.025.0519712
ACATGGG112750.023.8833373
GTATCAA186550.022.6818351
CATGGGG62750.021.2690534
GAGTACT89550.020.78663812-13
CAACGCA209800.019.8711135
AGAGTAC148700.019.83883310-11
AACGCAG210850.019.824246
TCAACGC210500.019.8050354
ATCAACG210600.019.795633
TATCAAC216900.019.373962
AGTACTT95650.019.14074312-13
GGTATCA140000.018.834961
CATGGGA54350.018.742594
GTACTTT99200.018.64565514-15
ACGCAGA229250.018.1303337
CGCAGAG229150.017.8505928
GAGTACA90750.017.3092631
GCAGAGT238750.016.7380649
Produced by FastQC (version 0.11.5)