##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939779_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3500668 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.428862434255404 31.0 31.0 34.0 30.0 34.0 2 31.637516611115363 31.0 31.0 34.0 30.0 34.0 3 31.703631135543272 31.0 31.0 34.0 30.0 34.0 4 35.5081750111693 37.0 35.0 37.0 33.0 37.0 5 35.23393935100387 37.0 35.0 37.0 33.0 37.0 6 35.25450171224463 37.0 35.0 37.0 32.0 37.0 7 35.173279499798326 37.0 35.0 37.0 32.0 37.0 8 35.11464069143375 37.0 35.0 37.0 32.0 37.0 9 36.72682870812085 39.0 35.0 39.0 32.0 39.0 10-11 36.578795532738326 38.5 35.0 39.0 32.0 39.0 12-13 36.52139591643652 38.0 35.0 39.0 32.0 39.0 14-15 37.57032743464961 39.0 36.0 41.0 32.0 41.0 16-17 37.50002528088925 39.0 36.0 41.0 32.0 41.0 18-19 37.46491112553375 39.0 36.0 41.0 32.0 41.0 20-21 37.383956147798074 39.0 36.0 41.0 32.0 41.0 22-23 37.240927874337125 39.0 36.0 40.0 31.0 41.0 24-25 37.06865746766046 39.0 36.0 40.0 31.0 41.0 26-27 36.77949051438183 39.0 36.0 40.0 30.0 41.0 28-29 36.56068741737292 38.0 35.0 40.0 30.0 41.0 30-31 36.319271350496535 38.0 35.0 40.0 30.0 41.0 32-33 36.29289167096108 38.0 35.0 40.0 30.0 41.0 34-35 36.38222590659839 38.0 35.0 40.0 30.0 41.0 36-37 36.36150600399695 38.0 35.0 40.0 30.0 41.0 38-39 36.242742099507865 38.0 35.0 40.0 30.0 41.0 40-41 36.09394278463425 38.0 35.0 40.0 29.5 41.0 42-43 35.90852788667763 38.0 35.0 40.0 29.0 41.0 44-45 35.68065980549998 38.0 34.5 40.0 28.0 41.0 46-47 35.407191570294586 38.0 34.0 40.0 27.0 41.0 48-49 35.121171587822666 38.0 34.0 40.0 26.5 41.0 50-51 34.85655152102399 38.0 33.5 40.0 26.0 41.0 52-53 34.58019026654341 38.0 33.0 40.0 25.5 41.0 54-55 34.27158645150011 37.5 33.0 40.0 24.5 41.0 56-57 32.83996340127084 36.5 31.5 39.5 16.5 41.0 58-59 29.275297600343706 34.5 23.5 38.5 7.5 40.0 60-61 29.263206907938713 34.0 24.0 38.0 9.0 40.0 62-63 31.303465224351466 35.0 29.5 38.0 12.5 40.0 64-65 30.8863692586672 35.0 29.5 38.0 4.5 40.0 66-67 25.31234124458532 28.5 18.0 36.0 2.0 39.0 68-69 17.557345198116472 13.0 7.0 29.0 2.0 35.5 70-71 16.392391680673516 14.0 7.5 26.0 2.0 33.5 72-73 18.51541063019972 18.5 7.0 30.0 2.0 34.5 74-75 19.221183071345237 18.0 7.5 30.5 2.0 35.0 76-77 20.15566300488935 23.0 8.5 31.0 2.0 34.5 78-79 23.229236105794662 28.0 12.0 34.0 2.0 36.0 80-81 25.925446086289817 31.5 22.0 34.5 2.0 36.0 82-83 27.006838409126487 33.0 26.0 35.0 2.0 36.0 84-85 27.0285846872654 33.0 26.0 35.0 2.0 36.0 86-87 26.74724952494781 33.0 25.5 35.0 2.0 35.5 88-89 26.308728219871178 33.0 24.5 35.0 2.0 35.0 90-91 24.588901889582218 31.5 12.0 34.5 2.0 35.0 92-93 22.368806467794148 30.5 2.0 34.0 2.0 35.0 94-95 17.904329116614313 21.0 2.0 33.0 2.0 35.0 96-97 16.574048295925234 13.5 2.0 32.5 2.0 34.5 98-99 15.486161926809398 2.0 2.0 32.0 2.0 34.0 100-101 14.857121269426292 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2794866344010245 1101 2 0.2774838906992443 1101 3 0.3306089570067634 1101 4 0.22729864363832775 1101 5 0.2553492010972107 1101 6 0.3009266739205856 1101 7 0.3065442857432288 1101 8 0.28001558744063715 1101 9 0.34967903528156796 1101 10-11 0.3855727984109407 1101 12-13 0.3642023507776315 1101 14-15 0.38271591039541164 1101 16-17 0.3727445522512909 1101 18-19 0.4426966852143792 1101 20-21 0.506531032612564 1101 22-23 0.36653176449545555 1101 24-25 0.34397057329709924 1101 26-27 0.3529433241936033 1101 28-29 0.1173527228543989 1101 30-31 0.4332056294055846 1101 32-33 0.33982823598480394 1101 34-35 0.5813899418061439 1101 36-37 0.43372406438236055 1101 38-39 0.5068932979456306 1101 40-41 0.5013679248245353 1101 42-43 0.5165710912848525 1101 44-45 0.2757936232862761 1101 46-47 0.11454482878833971 1101 48-49 0.04776441615050686 1101 50-51 0.008157266994196277 1101 52-53 0.27142996725612534 1101 54-55 0.3364496296705468 1101 56-57 0.9037522969329501 1101 58-59 -8.132417041230596 1101 60-61 -10.001592176558901 1101 62-63 -3.004420197587738 1101 64-65 -0.5951145352580873 1101 66-67 -1.5177657895697543 1101 68-69 -4.129379242095387 1101 70-71 -4.5893095476610135 1101 72-73 -6.812814156716268 1101 74-75 -7.809896151277542 1101 76-77 -8.917555076431476 1101 78-79 -12.096371158099089 1101 80-81 -8.271950958868047 1101 82-83 -4.246074154506193 1101 84-85 -3.2961499505613254 1101 86-87 -3.1178742129168775 1101 88-89 -3.4492423232985416 1101 90-91 -8.803185451090316 1101 92-93 -14.536918949856496 1101 94-95 -12.723944831608236 1101 96-97 -12.600627774521014 1101 98-99 -12.056564429237149 1101 100-101 -11.783907630884402 1102 1 0.49221096098298744 1102 2 0.546264181291793 1102 3 0.5083726750006683 1102 4 0.3892703109989881 1102 5 0.44609299730363006 1102 6 0.4560733692087595 1102 7 0.45678379414584214 1102 8 0.5525424521343325 1102 9 0.626957202566679 1102 10-11 0.6647073547201572 1102 12-13 0.6539997026034143 1102 14-15 0.7360549815475181 1102 16-17 0.8391999982810319 1102 18-19 0.7888202990657547 1102 20-21 0.7620179274185475 1102 22-23 0.7984342668256161 1102 24-25 0.8762253149889432 1102 26-27 0.92379083485379 1102 28-29 0.8619384191537733 1102 30-31 0.8669509319302549 1102 32-33 0.8406163176940922 1102 34-35 0.8377311022583953 1102 36-37 0.7237843917450064 1102 38-39 0.6617300552650462 1102 40-41 0.7477576966744195 1102 42-43 0.8316050119607326 1102 44-45 0.7453051478107326 1102 46-47 0.741322906021054 1102 48-49 0.6928505897487227 1102 50-51 0.6687740646745439 1102 52-53 0.6103127767670671 1102 54-55 0.5908195649271661 1102 56-57 1.1763781558983624 1102 58-59 -8.943419233608715 1102 60-61 -9.762097503591335 1102 62-63 -2.529734356542683 1102 64-65 0.17325119041850812 1102 66-67 -1.063091788408581 1102 68-69 -3.7791008259868377 1102 70-71 -4.209837748988608 1102 72-73 -6.849143153405805 1102 74-75 -7.571923497635405 1102 76-77 -8.77357832867916 1102 78-79 -11.764093435091258 1102 80-81 -7.518908715632502 1102 82-83 -3.2823824511133104 1102 84-85 -2.441801292736365 1102 86-87 -2.1660801283245092 1102 88-89 -2.2682267209282543 1102 90-91 -8.17954294348515 1102 92-93 -14.281921745775792 1102 94-95 -12.452161339262128 1102 96-97 -12.270753443735668 1102 98-99 -11.77414292514663 1102 100-101 -11.538631438978584 1103 1 0.36111191062996184 1103 2 0.33612411107275264 1103 3 0.2924081779613523 1103 4 0.18353692333478477 1103 5 0.2224364645999941 1103 6 0.2511898639648962 1103 7 0.3621298166872293 1103 8 0.4130984716031705 1103 9 0.4386906979211531 1103 10-11 0.4480347408017096 1103 12-13 0.41564495768276544 1103 14-15 0.5231984418777742 1103 16-17 0.5138561668635901 1103 18-19 0.476632851877973 1103 20-21 0.5120814131466531 1103 22-23 0.4390553767143075 1103 24-25 0.42833203711906265 1103 26-27 0.2921415156216085 1103 28-29 0.429662674824705 1103 30-31 0.5244492050651317 1103 32-33 0.4761583027741878 1103 34-35 0.5480036528421763 1103 36-37 0.3036734281107343 1103 38-39 0.35356184554125747 1103 40-41 0.41877052194659115 1103 42-43 0.5305113070260461 1103 44-45 0.4754764535411198 1103 46-47 0.388716285983449 1103 48-49 0.41169806744164106 1103 50-51 0.4106544221991584 1103 52-53 0.43805587112809263 1103 54-55 0.5194962593474699 1103 56-57 1.066731150763168 1103 58-59 -6.604026574780221 1103 60-61 -8.567403968615366 1103 62-63 -2.3445272953303586 1103 64-65 -0.01203463930042048 1103 66-67 -1.6161447544315877 1103 68-69 -3.8595647436133724 1103 70-71 -4.773646325790523 1103 72-73 -7.696341491350331 1103 74-75 -8.260785364241983 1103 76-77 -9.119124616039112 1103 78-79 -12.447785191045188 1103 80-81 -8.688055486994497 1103 82-83 -4.643921410914093 1103 84-85 -3.663245998666387 1103 86-87 -3.39235157108455 1103 88-89 -3.4488371755216107 1103 90-91 -9.044682508128549 1103 92-93 -14.939074201620556 1103 94-95 -12.83556382420637 1103 96-97 -12.48763912801941 1103 98-99 -12.129989183238283 1103 100-101 -11.863403721136349 1104 1 0.13551718049128425 1104 2 0.13391080384544551 1104 3 0.11597283517242118 1104 4 0.16931513184744063 1104 5 0.06193976749052155 1104 6 0.16395384741189645 1104 7 0.1718059033312187 1104 8 0.24373473455213457 1104 9 0.17233809333607297 1104 10-11 0.27370111567062594 1104 12-13 0.11181430117950697 1104 14-15 0.15065202088477037 1104 16-17 0.27227747359560794 1104 18-19 0.2738522207792684 1104 20-21 0.2490886249462747 1104 22-23 0.324730017367024 1104 24-25 0.3725738635328355 1104 26-27 0.3124527371151373 1104 28-29 0.32070523810468643 1104 30-31 0.2930839785656971 1104 32-33 0.3558328088884011 1104 34-35 0.1865486218908785 1104 36-37 0.17825541370261533 1104 38-39 0.3152863850966199 1104 40-41 0.43375325964442624 1104 42-43 0.31184225894848083 1104 44-45 0.2902196859120352 1104 46-47 0.2536266794698747 1104 48-49 0.26766723133543024 1104 50-51 0.2085708166386695 1104 52-53 0.21389388676849563 1104 54-55 0.061395776348284414 1104 56-57 0.5961602580495935 1104 58-59 -6.840371883925592 1104 60-61 -9.015835091895795 1104 62-63 -2.8417871150785246 1104 64-65 -0.5711161669553739 1104 66-67 -1.8403192401566635 1104 68-69 -4.1323784705889075 1104 70-71 -4.757896391199452 1104 72-73 -7.697750722656252 1104 74-75 -8.609972016474533 1104 76-77 -8.99736749256759 1104 78-79 -12.060499585742466 1104 80-81 -8.379093243201794 1104 82-83 -4.668016379025193 1104 84-85 -3.762254729679565 1104 86-87 -3.598800832060377 1104 88-89 -3.717504690693655 1104 90-91 -9.39677029569344 1104 92-93 -15.24425671709174 1104 94-95 -12.815334680130217 1104 96-97 -12.570928136074755 1104 98-99 -11.876736670240584 1104 100-101 -11.452001344745833 1105 1 0.08646703515184129 1105 2 0.0963565890316751 1105 3 0.07374713499826413 1105 4 0.11213144918737328 1105 5 0.06668415556021046 1105 6 0.030049457887159292 1105 7 0.10242582287668967 1105 8 0.1955603236135417 1105 9 0.11633708803025655 1105 10-11 0.15398547231248472 1105 12-13 0.11911028407487834 1105 14-15 0.12020702274716655 1105 16-17 0.12281812610041243 1105 18-19 0.17528392476962296 1105 20-21 0.1066204969585769 1105 22-23 0.1654046785798613 1105 24-25 0.1974006782616513 1105 26-27 0.24114967859846814 1105 28-29 0.2351157287594603 1105 30-31 0.19467914997123614 1105 32-33 0.26287441393849775 1105 34-35 0.189982640128882 1105 36-37 0.06383814384744824 1105 38-39 0.2696764209046236 1105 40-41 0.22530096462205051 1105 42-43 0.2371233702924016 1105 44-45 0.2880149426362948 1105 46-47 0.18139715528557332 1105 48-49 0.3325592105643267 1105 50-51 0.2297248529986362 1105 52-53 0.20085046471231038 1105 54-55 0.0847321590691692 1105 56-57 0.6472366477631226 1105 58-59 -6.863214933261034 1105 60-61 -8.894726706160192 1105 62-63 -2.771556177333231 1105 64-65 -0.26554611436621656 1105 66-67 -1.5012723386120008 1105 68-69 -4.150771899304036 1105 70-71 -4.837157118645234 1105 72-73 -6.112233965705467 1105 74-75 -6.584026919279438 1105 76-77 -8.529175696211762 1105 78-79 -11.980140657896307 1105 80-81 -8.080137570754719 1105 82-83 -4.065311267628349 1105 84-85 -3.182888706671168 1105 86-87 -2.9539909487979052 1105 88-89 -3.071591270853112 1105 90-91 -8.352028850788798 1105 92-93 -11.437222748036762 1105 94-95 -11.58549896826457 1105 96-97 -11.971327552732827 1105 98-99 -11.485425365450439 1105 100-101 -11.26324901524122 1106 1 0.1319291535370155 1106 2 0.19160170354128425 1106 3 0.08943180041901044 1106 4 0.03302358827424001 1106 5 -0.042105933641309434 1106 6 -7.846018891584094E-4 1106 7 0.07409005602385577 1106 8 0.16312246352104154 1106 9 -0.018818159159067704 1106 10-11 0.10565213639853965 1106 12-13 0.09660288674333373 1106 14-15 0.2309794884159615 1106 16-17 0.09137075747561596 1106 18-19 0.2097406254126568 1106 20-21 0.15272418192128612 1106 22-23 0.19753425846098338 1106 24-25 0.06527721443696066 1106 26-27 0.16838246499635545 1106 28-29 0.20490937766066253 1106 30-31 0.32469249690957014 1106 32-33 0.2394870012471344 1106 34-35 0.21342440056218237 1106 36-37 0.18679936804969088 1106 38-39 0.26581757639026904 1106 40-41 0.32715374262777885 1106 42-43 0.17620280315590975 1106 44-45 0.23256863309571685 1106 46-47 0.1329045696491704 1106 48-49 0.2436337629696439 1106 50-51 0.3303594519169408 1106 52-53 0.24673381680671014 1106 54-55 0.02551317173328016 1106 56-57 0.9790724246803535 1106 58-59 -6.462991503655296 1106 60-61 -8.653175534896679 1106 62-63 -2.2621363665748575 1106 64-65 0.13075467793405338 1106 66-67 -1.343600016786656 1106 68-69 -4.112393490376764 1106 70-71 -4.728741657254581 1106 72-73 -6.125688205990613 1106 74-75 -6.614089426924508 1106 76-77 -8.673813617929337 1106 78-79 -11.993206567307446 1106 80-81 -7.846159006371227 1106 82-83 -3.87472511716717 1106 84-85 -2.8644041261010287 1106 86-87 -2.7037122424290025 1106 88-89 -2.7796283109295388 1106 90-91 -8.224074447150752 1106 92-93 -11.675586834553412 1106 94-95 -11.902135570772 1106 96-97 -12.259208935302222 1106 98-99 -11.73961323831698 1106 100-101 -11.458129101825998 1107 1 0.021794543470537775 1107 2 -0.04089520272913916 1107 3 -0.036709866904253374 1107 4 0.03642352369600843 1107 5 5.600347153560392E-4 1107 6 0.07350322233447315 1107 7 0.12370297290487997 1107 8 0.034842608065027036 1107 9 0.054275894854903584 1107 10-11 0.06580374073485729 1107 12-13 0.08799590704222027 1107 14-15 0.012005926865036542 1107 16-17 0.10296123574072169 1107 18-19 0.013137625460551305 1107 20-21 0.030909950919728146 1107 22-23 0.013340010866826901 1107 24-25 0.053008896545243545 1107 26-27 0.1936550299106088 1107 28-29 0.23579260076298425 1107 30-31 0.20819886280855826 1107 32-33 0.11033997454914157 1107 34-35 0.06778188584281963 1107 36-37 0.10795051354072882 1107 38-39 0.21112213687982262 1107 40-41 -0.12207881159552869 1107 42-43 0.012693208345659457 1107 44-45 0.043298428840039094 1107 46-47 0.08311329018148683 1107 48-49 0.07097916532442383 1107 50-51 0.10000266494629528 1107 52-53 0.12702316742475972 1107 54-55 0.05397679704361735 1107 56-57 0.6662908798394227 1107 58-59 -6.770259334892927 1107 60-61 -9.004035831184048 1107 62-63 -2.640612505847532 1107 64-65 -0.2129171194571704 1107 66-67 -1.7345068464747193 1107 68-69 -4.301292833316493 1107 70-71 -4.934692200839477 1107 72-73 -6.34786881800062 1107 74-75 -6.933742836515766 1107 76-77 -9.236677044252225 1107 78-79 -12.559062839042731 1107 80-81 -8.615906579666738 1107 82-83 -4.457214839496249 1107 84-85 -3.5769152303114353 1107 86-87 -3.310618945432509 1107 88-89 -3.5487535077154604 1107 90-91 -8.912998431781752 1107 92-93 -12.078174752863504 1107 94-95 -12.157954486012306 1107 96-97 -12.757380197373948 1107 98-99 -11.979730838664283 1107 100-101 -11.420176494209784 1108 1 -0.003125256862240633 1108 2 0.008978119511571236 1108 3 0.0036535274385087746 1108 4 -0.04068263270998074 1108 5 -0.1942299819235771 1108 6 -0.054432884154714145 1108 7 -0.09549880191486437 1108 8 -0.049883293210619684 1108 9 -0.00474573576073567 1108 10-11 0.039403297029920736 1108 12-13 -0.08242849672927122 1108 14-15 0.10559638720890518 1108 16-17 0.004824796472831849 1108 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1.0188608464472253 2314 64-65 0.8599170268935303 2314 66-67 1.6306944814366524 2314 68-69 -0.25912583007068335 2314 70-71 2.4957344589077692 2314 72-73 8.318208816967037 2314 74-75 9.735021679961324 2314 76-77 8.580393065991093 2314 78-79 6.218543239446969 2314 80-81 3.174557797621077 2314 82-83 1.6083693863214883 2314 84-85 1.1453180097873634 2314 86-87 1.0608713087702917 2314 88-89 1.1894837320181466 2314 90-91 2.5583091246711582 2314 92-93 4.659174334383081 2314 94-95 8.528968740889798 2314 96-97 9.13399105065685 2314 98-99 9.470575941246741 2314 100-101 8.68786075067844 2315 1 -0.7983809489179343 2315 2 -0.5310474333139794 2315 3 -0.5397532975683141 2315 4 -0.4698089226362683 2315 5 -0.6995877760813727 2315 6 -0.7165336758555014 2315 7 -0.6738452938613548 2315 8 -0.9067337436610714 2315 9 -1.0677170707320727 2315 10-11 -0.807006749380129 2315 12-13 -0.6209217536225324 2315 14-15 -0.848696705884187 2315 16-17 -0.9734061617581204 2315 18-19 -0.824100838233818 2315 20-21 -0.9101961453444432 2315 22-23 -0.810210850537608 2315 24-25 -0.91198676770167 2315 26-27 -0.8904798158714939 2315 28-29 -0.874437505307391 2315 30-31 -0.9146613669568282 2315 32-33 -0.7641201004101035 2315 34-35 -0.951248413553536 2315 36-37 -1.1281834800807928 2315 38-39 -1.0553829099912733 2315 40-41 -0.7704793297963803 2315 42-43 -0.7226154250495043 2315 44-45 -0.7201956657688626 2315 46-47 -0.6868547946784389 2315 48-49 -0.6319591761330514 2315 50-51 -0.6888727329141275 2315 52-53 -0.7101644210107452 2315 54-55 -0.8110073067092003 2315 56-57 0.6279430856680079 2315 58-59 4.086855456498071 2315 60-61 3.817856360508472 2315 62-63 1.190527059069069 2315 64-65 1.1447122161255798 2315 66-67 1.9184882299812536 2315 68-69 6.084584989749349 2315 70-71 4.960814219209372 2315 72-73 9.058205120103267 2315 74-75 10.116801846724425 2315 76-77 8.86989129234939 2315 78-79 6.217372649539179 2315 80-81 3.24192091959171 2315 82-83 1.513900505163189 2315 84-85 0.9929106082332062 2315 86-87 1.0239971712135585 2315 88-89 1.157522273435525 2315 90-91 2.3025520907571213 2315 92-93 4.230920153569176 2315 94-95 8.29343057019804 2315 96-97 9.023045932885797 2315 98-99 9.066248130775586 2315 100-101 7.970124183112526 2316 1 -0.524367061042657 2316 2 -0.5073747957799561 2316 3 -0.715021500064239 2316 4 -0.40650529497620624 2316 5 -0.6133507740027824 2316 6 -0.5527206729335887 2316 7 -0.71297690972564 2316 8 -0.7497376386451649 2316 9 -0.768224283615524 2316 10-11 -0.7830114444442273 2316 12-13 -0.7838579796775704 2316 14-15 -0.9251580682366338 2316 16-17 -1.0198506710739181 2316 18-19 -1.0537308958341711 2316 20-21 -0.9375373929827262 2316 22-23 -1.1523592545672088 2316 24-25 -2.49328678959575 2316 26-27 -1.7568596971028612 2316 28-29 -1.3602242596585015 2316 30-31 -1.0942860861855124 2316 32-33 -0.9504262310231653 2316 34-35 -0.999268080385086 2316 36-37 -1.0443291487017063 2316 38-39 -1.2867584124063853 2316 40-41 -1.1172717793116078 2316 42-43 -1.2570053505285372 2316 44-45 -1.1848265883205826 2316 46-47 -1.1560148863632804 2316 48-49 -1.437476214323354 2316 50-51 -1.3214843048821407 2316 52-53 -1.2185804653178778 2316 54-55 -1.0638633355553324 2316 56-57 0.04856926990805022 2316 58-59 3.4555254316036894 2316 60-61 3.4831813389522637 2316 62-63 0.7895325677280454 2316 64-65 0.48385727928054223 2316 66-67 5.45552571751379 2316 68-69 8.655313684240422 2316 70-71 5.466781369978502 2316 72-73 9.07579640625891 2316 74-75 9.762821516258946 2316 76-77 8.445436008983211 2316 78-79 5.899687840517739 2316 80-81 2.7453452485110894 2316 82-83 1.3158506326083668 2316 84-85 1.052958842786392 2316 86-87 0.7754500192010596 2316 88-89 0.9762433347308459 2316 90-91 2.441853858771765 2316 92-93 4.254871197322199 2316 94-95 8.342222611564342 2316 96-97 9.033512182213428 2316 98-99 9.243305025654264 2316 100-101 8.211398310525263 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 26.0 4 65.0 5 127.0 6 316.0 7 856.0 8 2011.0 9 3533.0 10 5574.0 11 8608.0 12 12088.0 13 16212.0 14 21018.0 15 29104.0 16 39195.0 17 50717.0 18 57147.0 19 59879.0 20 63878.0 21 63436.0 22 61326.0 23 59361.0 24 60939.0 25 65411.0 26 85430.0 27 124594.0 28 174107.0 29 229628.0 30 270780.0 31 287002.0 32 293990.0 33 301988.0 34 307198.0 35 296641.0 36 248633.0 37 151472.0 38 46515.0 39 1856.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.168582443350275 19.503836172450473 14.097908755296112 24.22967262890314 2 15.70747275940344 21.255207151945708 38.415254619802916 24.622065468847936 3 19.08174581363872 24.790160586927595 30.310661173409542 25.817432426024144 4 13.230217775578831 16.887148395677627 36.85319487594939 33.02943895279415 5 13.579807546357555 37.57928500639025 34.57376546359557 14.26714198365662 6 32.16771770416389 36.476495343174506 17.114247909256175 14.24153904340543 7 28.085896748849077 32.65542462181504 21.29282182714842 17.965856802187467 8 26.158638656710448 34.847314506407365 20.02976581558906 18.96428102129313 9 26.1033894102497 16.300460369278092 20.194974216349564 37.40117600412265 10-11 24.959422030309643 26.14258193007735 27.822504162062785 21.075491877550228 12-13 25.86633465384321 23.54714871561656 28.026608064517973 22.55990856602226 14-15 22.515806668945284 25.042288472297425 26.101989097194146 26.339915761563148 16-17 22.41824988830703 28.270490089320095 26.697833099282764 22.613426923090106 18-19 22.403285127655774 27.21886805927513 28.38764506338154 21.990201749687554 20-21 23.017306982553045 26.27057178801303 28.217300240982578 22.494820988451348 22-23 22.719549525976184 26.26580126992905 28.215700546295736 22.798948657799027 24-25 22.84509699291678 26.467634177248456 28.170680567251736 22.516588262583028 26-27 22.576872602953387 26.496088454126678 28.385202570256713 22.541836372663226 28-29 22.406611326606008 26.97845811223424 28.18827980415349 22.426650757006268 30-31 22.574031083713248 26.63082076841371 28.04062103432967 22.754527113543375 32-33 22.4145257242316 26.659412793335886 28.154717176782597 22.771344305649922 34-35 22.99615641162246 26.559167122536344 27.97345338774251 22.47122307809869 36-37 22.94526791138217 26.262421771910866 28.10690738811476 22.685402928592204 38-39 22.52922571743125 26.529676607682333 28.223000946069472 22.718096728816946 40-41 22.648218977749703 26.417783548494615 28.319096003999245 22.614901469756433 42-43 22.553906942481543 26.521084156608023 27.89179578939789 23.03321311151255 44-45 22.561853232426994 26.38348789795449 28.02145378848816 23.033205081130355 46-47 22.702596897895734 26.6927413732012 27.745930917029572 22.858730811873492 48-49 22.60813396472916 26.62093790398074 27.74138260141024 23.029545529879858 50-51 22.171591310583093 26.758761429636614 27.99944338539404 23.070203874386255 52-53 22.561674415999907 26.856332704833978 27.51221402160251 23.06977885756361 54-55 22.166845259276897 26.498581520871905 27.7518345087208 23.582738711130393 56-57 22.25246922489461 26.574551502876787 27.758037202049103 23.4149420701795 58-59 20.781948751122822 26.343835929234633 29.4442414904024 23.429973829240144 60-61 22.18483113986643 26.53268338750026 28.031550050900584 23.25093542173273 62-63 21.9517584359606 26.515077827611332 27.8490573260107 23.684106410417368 64-65 21.181328036849976 26.684395983929193 28.23735602617291 23.896919953047917 66-67 21.088346511724566 26.390646241041242 28.49178370185473 24.029223545379462 68-69 21.6146852568274 25.84855113751387 28.709954060529313 23.826809545129414 70-71 20.854464813167365 26.228208891099325 28.878224885644837 24.039101410088474 72-73 20.772560152650442 26.14281309629078 28.87498836505794 24.209638386000837 74-75 21.219104516052685 26.541678050625407 28.084980309376846 24.154237123945062 76-77 20.002717468209088 26.695139919290327 28.622910711676337 24.67923190082425 78-79 21.03582413112922 26.846385361604387 27.539551622550874 24.57823888471552 80-81 21.837291964407886 26.799523404566507 26.85327036621349 24.50991426481212 82-83 22.010946220006197 26.70666007172075 26.706360128324352 24.576033579948696 84-85 21.976672156573613 26.610832537175423 26.556842818360447 24.85565248789052 86-87 22.06427240936478 26.62108285059252 26.59430160877419 24.720343131268507 88-89 21.986295754494787 26.55205422280954 26.31509937963251 25.146550643063165 90-91 21.63048986123795 26.545878506626053 26.55874699043524 25.264884641700757 92-93 21.495658175498015 26.500209315228734 26.83012044710763 25.174012062165623 94-95 19.952763961989582 26.392908432698768 27.92587393106732 25.72845367424432 96-97 19.66141491588224 26.331130700141216 28.21854220174439 25.788912182232153 98-99 20.132241621973908 26.38734793201384 27.464084387501668 26.01632605851058 100-101 20.07749959493736 26.278198453449342 27.43365217378836 26.210649777824933 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 351.0 1 260.0 2 203.0 3 265.0 4 312.5 5 369.0 6 493.5 7 767.0 8 1113.5 9 1376.5 10 1581.5 11 1778.5 12 2012.0 13 2338.0 14 2673.0 15 2925.5 16 3173.5 17 3512.5 18 3889.0 19 4381.0 20 5060.0 21 5837.5 22 7024.5 23 8506.0 24 10237.0 25 12605.5 26 15731.0 27 19710.0 28 24153.0 29 29190.5 30 34748.5 31 40949.0 32 47419.5 33 53246.5 34 58886.5 35 65271.0 36 72520.0 37 79617.5 38 86624.5 39 93249.5 40 100038.0 41 107517.0 42 115126.0 43 122187.0 44 129236.5 45 136817.0 46 144217.0 47 150766.5 48 154837.0 49 154767.0 50 151679.5 51 147284.0 52 141533.0 53 134786.0 54 126956.0 55 116780.0 56 104352.0 57 91609.0 58 78666.5 59 65600.5 60 53510.0 61 42771.0 62 33271.0 63 25131.5 64 18713.0 65 13798.5 66 10018.0 67 7336.0 68 5163.0 69 3447.5 70 2436.0 71 1801.0 72 1360.0 73 1011.5 74 756.5 75 528.5 76 291.5 77 154.0 78 88.5 79 49.5 80 33.0 81 19.5 82 9.5 83 8.0 84 5.0 85 5.5 86 4.0 87 0.5 88 1.0 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019853353702779013 2 0.005541799450847667 3 0.0038278408578019964 4 0.0 5 0.06772993040185474 6 0.0 7 0.0 8 2.2852781240608933E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.713767772322311E-4 16-17 0.0 18-19 0.00122833699168273 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.71395859304567E-4 28-29 0.001985335370277901 30-31 0.008969716636939008 32-33 2.713767772322311E-4 34-35 9.426772261751186E-4 36-37 0.027594733348035288 38-39 0.02642352830945408 40-41 0.028751655398341115 42-43 0.02930869194108096 44-45 0.030079973307951507 46-47 0.03165110201824337 48-49 0.03199389373685251 50-51 0.026837734969440117 52-53 0.022767083310956653 54-55 0.01761092454354426 56-57 0.1482145693336243 58-59 0.7314746785470658 60-61 0.3008282990560659 62-63 4.14206659986037E-4 64-65 0.13607402929955084 66-67 1.3742948488688445 68-69 2.635782656338733 70-71 2.128165253031707 72-73 1.7924578966071618 74-75 1.5092548050829155 76-77 1.0822505876021378 78-79 0.4077793152621157 80-81 1.4282988275380584E-4 82-83 2.1424482413070876E-4 84-85 4.284896482614175E-5 86-87 0.002385259041988558 88-89 2.8565976550761166E-5 90-91 0.21818692889471383 92-93 0.1696533347349706 94-95 1.250432774544744 96-97 1.223680737504956 98-99 0.9642873874357694 100-101 0.3871546801924661 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3500668.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.36238624638343 #Duplication Level Percentage of deduplicated Percentage of total 1 63.54585692141818 24.37770707576862 2 14.628129508313876 11.223399085201116 3 7.070245131517085 8.136944237756111 4 4.012674856649028 6.157431309276851 5 2.557465218628161 4.905523426435248 6 1.7658175089174246 4.064458399063012 7 1.2846078676497765 3.449643623574661 8 0.9267536659193651 2.844198566980038 9 0.7069351750300495 2.4407748212091693 >10 3.2893005841400575 21.455605207513354 >50 0.1452893707200427 3.781891826519548 >100 0.06123162123977303 4.124289034335025 >500 0.003520405045573543 0.934094126769373 >1k 0.0018725558722238699 1.2441657912887178 >5k 2.996089395558192E-4 0.8598734683090806 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9578 0.2736049234031905 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8263 0.23604066423893955 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7009 0.20021892964428503 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5075 0.14497233099511295 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4419 0.1262330503778136 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3808 0.10877923870529853 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.4282988275380585E-5 0.0 12-13 0.0 0.0 0.0 2.856597655076117E-5 0.0 14-15 0.0 0.0 0.0 5.713195310152234E-5 0.0 16-17 0.0 0.0 0.0 7.141494137690292E-5 0.0 18-19 0.0 0.0 0.0 9.998091792766408E-5 0.0 20-21 0.0 0.0 0.0 2.1424482413070876E-4 0.0 22-23 0.0 0.0 0.0 6.141684958413652E-4 0.0 24-25 0.0 0.0 0.0 0.0014997137689149614 0.0 26-27 0.0 0.0 0.0 0.003399351209540579 0.0 28-29 0.0 0.0 0.0 0.012511897729233393 0.0 30-31 0.0 0.0 0.0 0.0434060013688816 0.0 32-33 0.0 0.0 0.0 0.11619210962022106 0.0 34-35 0.0 0.0 0.0 0.2253855549855056 0.0 36-37 0.0 0.0 0.0 0.3986667687424229 0.0 38-39 0.0 0.0 0.0 0.6647017083596616 0.0 40-41 0.0 0.0 0.0 0.9613879408158671 0.0 42-43 0.0 0.0 0.0 1.2641158773125585 0.0 44-45 0.0 0.0 0.0 1.6140490900593831 0.0 46-47 0.0 0.0 0.0 2.0087166220846995 0.0 48-49 0.0 0.0 0.0 2.3779461519915626 0.0 50-51 0.0 1.4282988275380585E-5 0.0 2.7492038662335303 0.0 52-53 0.0 2.856597655076117E-5 0.0 3.1010081504444296 0.0 54-55 2.856597655076117E-5 2.856597655076117E-5 0.0 3.4812212983350603 0.0 56-57 5.713195310152234E-5 2.856597655076117E-5 0.0 3.8129865499956006 0.0 58-59 8.56979296522835E-5 4.284896482614176E-5 0.0 4.033358776096447 0.0 60-61 8.56979296522835E-5 5.713195310152234E-5 0.0 4.195684937846148 0.0 62-63 8.56979296522835E-5 5.713195310152234E-5 0.0 4.335001205484211 5.713195310152234E-5 64-65 1.1426390620304468E-4 8.56979296522835E-5 0.0 4.480330611186208 8.56979296522835E-5 66-67 1.1426390620304468E-4 8.56979296522835E-5 0.0 4.601078994066276 8.56979296522835E-5 68-69 1.1426390620304468E-4 8.56979296522835E-5 0.0 4.73843849231061 8.56979296522835E-5 70-71 1.4282988275380584E-4 9.998091792766408E-5 0.0 4.982820421702372 8.56979296522835E-5 72-73 1.4282988275380584E-4 1.1426390620304468E-4 0.0 5.2851341515390775 8.56979296522835E-5 74-75 1.4282988275380584E-4 1.1426390620304468E-4 0.0 5.643322931509072 8.56979296522835E-5 76-77 1.4282988275380584E-4 1.1426390620304468E-4 0.0 6.149997657589923 8.56979296522835E-5 78-79 1.4282988275380584E-4 1.1426390620304468E-4 0.0 6.863989958487923 8.56979296522835E-5 80-81 1.4282988275380584E-4 1.1426390620304468E-4 0.0 7.650382726953827 8.56979296522835E-5 82-83 1.4282988275380584E-4 1.1426390620304468E-4 0.0 8.37587283341351 8.56979296522835E-5 84-85 1.4282988275380584E-4 1.1426390620304468E-4 0.0 8.974415740081607 8.56979296522835E-5 86-87 1.4282988275380584E-4 1.1426390620304468E-4 0.0 9.501100932736268 8.56979296522835E-5 88-89 1.4282988275380584E-4 1.1426390620304468E-4 0.0 10.028414576875042 8.56979296522835E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10935 0.0 26.443727 1 TACATGG 11145 0.0 25.051971 2 ACATGGG 11275 0.0 23.883337 3 GTATCAA 18655 0.0 22.681835 1 CATGGGG 6275 0.0 21.269053 4 GAGTACT 8955 0.0 20.786638 12-13 CAACGCA 20980 0.0 19.871113 5 AGAGTAC 14870 0.0 19.838833 10-11 AACGCAG 21085 0.0 19.82424 6 TCAACGC 21050 0.0 19.805035 4 ATCAACG 21060 0.0 19.79563 3 TATCAAC 21690 0.0 19.37396 2 AGTACTT 9565 0.0 19.140743 12-13 GGTATCA 14000 0.0 18.83496 1 CATGGGA 5435 0.0 18.74259 4 GTACTTT 9920 0.0 18.645655 14-15 ACGCAGA 22925 0.0 18.130333 7 CGCAGAG 22915 0.0 17.850592 8 GAGTACA 9075 0.0 17.309263 1 GCAGAGT 23875 0.0 16.738064 9 >>END_MODULE