FastQCFastQC Report
Fri 27 May 2016
SRR939778_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939778_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3633499
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86690.23858545165417688No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT77530.21337559195695388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58520.16105687658094855No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43460.11960922515734833No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT39990.11005920188776715No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG117150.027.58411
TACATGG122400.025.9412362
ACATGGG126300.024.0231913
CATGGGG67600.020.9456634
GTATCAA201150.020.8120251
GAGTACT87250.020.56839812-13
AGAGTAC151500.018.80116710-11
AGTACTT96700.018.3880912-13
GGTATCA162200.018.0378151
ATCAACG230600.018.0125713
TCAACGC231900.017.9927354
CAACGCA231850.017.9768225
GTACTTT100300.017.94322814-15
TATCAAC232400.017.812092
CATGGGA63100.017.742794
AACGCAG235900.017.608856
GAGTACA96250.017.3489041
AGTACAT88700.016.8114032
ACCGTCG2658.712595E-615.9763268
ACGCAGA259600.015.7294417
Produced by FastQC (version 0.11.5)