FastQCFastQC Report
Fri 27 May 2016
SRR939778_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939778_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3633499
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96060.2643732666501353No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT86140.23707175920510778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69940.1924866361598008No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52950.14572730032401277No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47620.1310582444084889No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT38940.10716942539408983No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG115600.024.426511
TACATGG121300.022.5701072
ACATGGG119650.021.6073153
GAGTACT86950.021.14068612-13
GTATCAA209100.020.3809571
AGTACTT94500.019.97874612-13
TACCGGA2702.3319444E-819.3306982
GTACTTT96950.018.96011214-15
AGAGTAC159750.018.44028910-11
CATGGGG64450.018.179884
TCAACGC234550.018.0808584
ATCAACG234050.018.0586813
CAACGCA235050.017.9818535
ATTCGCG4650.017.85434580-81
GAGTACA103200.017.7964571
AACGCAG238000.017.77896
TATCAAC239850.017.7250272
CGCGCTT5600.017.4822462-63
AGTACAT94950.017.290192
GGTATCA168200.017.1263561
Produced by FastQC (version 0.11.5)