##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939778_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3633499 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.40129225300461 31.0 31.0 34.0 30.0 34.0 2 31.628042005791112 31.0 31.0 34.0 30.0 34.0 3 31.679011608369784 31.0 31.0 34.0 30.0 34.0 4 35.48679606076677 37.0 35.0 37.0 33.0 37.0 5 35.21636499693546 37.0 35.0 37.0 32.0 37.0 6 35.236946535557045 37.0 35.0 37.0 32.0 37.0 7 35.143416579996305 37.0 35.0 37.0 32.0 37.0 8 35.10587122770641 37.0 35.0 37.0 32.0 37.0 9 36.69476666981331 39.0 35.0 39.0 32.0 39.0 10-11 36.555932174468744 38.0 35.0 39.0 32.0 39.0 12-13 36.47642300163011 38.0 35.0 39.0 32.0 39.0 14-15 37.517916476652395 39.0 36.0 41.0 32.0 41.0 16-17 37.441452027370865 39.0 36.0 41.0 32.0 41.0 18-19 37.40403355002987 39.0 36.0 41.0 32.0 41.0 20-21 37.31459180255726 39.0 36.0 40.5 31.0 41.0 22-23 37.18627912103457 39.0 36.0 40.0 31.0 41.0 24-25 37.02736122949256 39.0 36.0 40.0 31.0 41.0 26-27 36.72960994347322 39.0 35.5 40.0 30.0 41.0 28-29 36.51137966461529 38.0 35.0 40.0 30.0 41.0 30-31 36.26392053499946 38.0 35.0 40.0 30.0 41.0 32-33 36.26369072896401 38.0 35.0 40.0 30.0 41.0 34-35 36.1181858038216 38.0 35.0 40.0 30.0 41.0 36-37 36.18286574456192 38.0 35.0 40.0 30.0 41.0 38-39 36.09659986145586 38.0 35.0 40.0 30.0 41.0 40-41 36.03468282226031 38.0 35.0 40.0 29.0 41.0 42-43 35.82713535905748 38.0 35.0 40.0 28.5 41.0 44-45 35.599177129263005 38.0 34.0 40.0 27.5 41.0 46-47 35.346492595704575 38.0 34.0 40.0 27.0 41.0 48-49 35.065656960411985 38.0 34.0 40.0 26.0 41.0 50-51 34.84495399613431 38.0 33.0 40.0 26.0 41.0 52-53 34.604723298396394 38.0 33.0 40.0 26.0 41.0 54-55 34.34714320824087 37.5 33.0 40.0 24.5 41.0 56-57 33.968582487569144 37.0 32.5 40.0 23.5 41.0 58-59 33.59946239148545 37.0 32.0 39.5 23.0 41.0 60-61 33.266106306895914 36.0 32.0 39.0 23.0 41.0 62-63 32.96708007900924 36.0 31.0 39.0 22.0 40.0 64-65 32.527971109941134 35.5 31.0 39.0 21.5 40.0 66-67 31.609029478197186 35.0 30.0 38.0 16.0 40.0 68-69 29.7095949386528 34.0 27.0 37.0 9.5 39.5 70-71 29.033307839082934 33.5 25.5 36.5 9.0 39.0 72-73 29.597964524002897 34.0 27.5 36.0 10.0 39.0 74-75 29.699239218175098 34.0 28.0 36.0 11.0 39.0 76-77 29.202159406126164 32.5 28.0 35.0 10.5 37.0 78-79 29.733939241485963 34.0 29.0 35.0 9.5 37.0 80-81 29.538528977164987 34.0 29.0 35.0 7.5 37.0 82-83 29.094606328500433 33.5 29.0 35.0 7.0 36.5 84-85 28.740028138166544 33.0 28.5 35.0 2.0 36.0 86-87 28.368302426944386 33.0 27.5 35.0 2.0 35.5 88-89 27.918739760214603 33.0 26.5 35.0 2.0 35.0 90-91 27.427101810128473 33.0 26.0 35.0 2.0 35.0 92-93 26.938565966304104 32.0 25.0 34.5 2.0 35.0 94-95 26.129741194369394 32.0 24.0 34.0 2.0 35.0 96-97 25.592244417846267 32.0 23.0 34.0 2.0 35.0 98-99 24.914657469287867 31.5 19.0 34.0 2.0 35.0 100-101 23.425143917749807 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.29822528352326927 1101 2 0.33931153672332215 1101 3 0.3373729371347238 1101 4 0.22453584362720136 1101 5 0.28057947284626295 1101 6 0.214465484858124 1101 7 0.19231677977377615 1101 8 0.21193688622771845 1101 9 0.2878852684885942 1101 10-11 0.31006035485226846 1101 12-13 0.3822284465014647 1101 14-15 0.4350884283153462 1101 16-17 0.46808659342185877 1101 18-19 0.422486541827908 1101 20-21 0.3648243342225683 1101 22-23 0.4554829821063606 1101 24-25 0.3031097351764629 1101 26-27 0.4074165301158317 1101 28-29 0.28191850243266714 1101 30-31 0.1925128426678171 1101 32-33 0.1351986456221752 1101 34-35 0.20989575328700738 1101 36-37 0.37221932818910375 1101 38-39 0.3016130264086172 1101 40-41 0.18967731679899913 1101 42-43 0.11079377041156846 1101 44-45 0.1330306837749049 1101 46-47 0.11599551645194595 1101 48-49 0.1276965623141919 1101 50-51 0.047168419930663674 1101 52-53 -0.10208810727178985 1101 54-55 -0.29351027568761623 1101 56-57 -0.18346961503844028 1101 58-59 -0.18580440885043714 1101 60-61 -0.25418938010086833 1101 62-63 -0.1874212917464675 1101 64-65 -0.4516539476119803 1101 66-67 -0.37821108813472293 1101 68-69 -8.899846076897184 1101 70-71 -6.803272734760185 1101 72-73 -1.794920619123225 1101 74-75 -0.6971801180711275 1101 76-77 -0.4799923559524153 1101 78-79 -0.6348211971703783 1101 80-81 -0.7241310183004472 1101 82-83 -0.544748347846987 1101 84-85 -0.43034305557055674 1101 86-87 -0.618089702529776 1101 88-89 -0.5725487456252409 1101 90-91 -0.9007485605800021 1101 92-93 -1.1398390621285266 1101 94-95 -0.7952706887518453 1101 96-97 -1.3073412218088691 1101 98-99 -1.1376248147255659 1101 100-101 -1.1449745700992935 1102 1 0.15360877221120006 1102 2 0.05231141775461268 1102 3 0.20625690247002382 1102 4 0.16787583611827017 1102 5 0.25972194019552575 1102 6 0.19028656209663097 1102 7 0.28382179577275224 1102 8 0.21582021036801535 1102 9 0.2739408994940078 1102 10-11 0.29775458672383337 1102 12-13 0.24817195335916153 1102 14-15 0.2680317914835584 1102 16-17 0.40774635835822437 1102 18-19 0.34551625773989514 1102 20-21 0.2910303602195441 1102 22-23 0.3384979582585359 1102 24-25 0.26050302256006574 1102 26-27 0.2930740022006759 1102 28-29 0.17188465042665513 1102 30-31 0.21151114156975126 1102 32-33 0.16969663628699294 1102 34-35 0.0075390912709352165 1102 36-37 0.04382002902296023 1102 38-39 0.29952381223558433 1102 40-41 0.27528511558428903 1102 42-43 0.33168653016121397 1102 44-45 0.319399959564457 1102 46-47 0.2500500010315889 1102 48-49 0.36060245806361735 1102 50-51 0.23428596256240297 1102 52-53 0.17848546097066986 1102 54-55 -0.044673233466085094 1102 56-57 -0.07888561154044993 1102 58-59 -0.08155823014089236 1102 60-61 0.07206655095290415 1102 62-63 0.20751728486242627 1102 64-65 0.012155924936990914 1102 66-67 0.4322508194744863 1102 68-69 -4.253323825885563 1102 70-71 0.3951286811219852 1102 72-73 0.6294030746544017 1102 74-75 0.42575550440561116 1102 76-77 0.1511134041417712 1102 78-79 0.06430268324907118 1102 80-81 0.10350240047404924 1102 82-83 0.08215325884863489 1102 84-85 0.011766228778569143 1102 86-87 -0.23009047814397476 1102 88-89 -0.28991562813514093 1102 90-91 -0.3988798247847889 1102 92-93 -0.4435025259102794 1102 94-95 -0.05099671462522437 1102 96-97 -0.6487213979134587 1102 98-99 -0.4816764564165332 1102 100-101 -0.5720815909528127 1103 1 0.1778792342111366 1103 2 0.2065876208191817 1103 3 0.1283800282794445 1103 4 0.10885858177130103 1103 5 0.16094136012547722 1103 6 0.07942793915105995 1103 7 0.14380167979434333 1103 8 0.23185424731555315 1103 9 0.3635976372341645 1103 10-11 0.3716972739871238 1103 12-13 0.3177489837601044 1103 14-15 0.25158720165814685 1103 16-17 0.24989534121068857 1103 18-19 0.32528879586902093 1103 20-21 0.21082479450216596 1103 22-23 0.17995022508898018 1103 24-25 0.24344516241951908 1103 26-27 0.2904377854287574 1103 28-29 0.22709811567096239 1103 30-31 0.16424415536166492 1103 32-33 0.26347047394392575 1103 34-35 0.16470318335938572 1103 36-37 0.29553634032217957 1103 38-39 0.2562003588739472 1103 40-41 0.32697005129771384 1103 42-43 0.2187317510586908 1103 44-45 0.24738275417231392 1103 46-47 0.14383540982655063 1103 48-49 -0.024664590450605317 1103 50-51 -0.11992303889288536 1103 52-53 -0.10263778500057441 1103 54-55 -0.13154199450872994 1103 56-57 0.0032676882365834103 1103 58-59 -0.17054450944146282 1103 60-61 -0.11151244654150361 1103 62-63 -0.27335962938209235 1103 64-65 -0.359329230628056 1103 66-67 -0.026911296619633873 1103 68-69 -4.255057716413699 1103 70-71 0.21558823331894317 1103 72-73 0.44230533859206034 1103 74-75 0.23480836225418145 1103 76-77 0.0061633158005811595 1103 78-79 -0.09837416601905247 1103 80-81 -0.0015114626393888386 1103 82-83 -0.15668704609535666 1103 84-85 -0.24440474813700774 1103 86-87 -0.2660825029596161 1103 88-89 -0.3377958846583695 1103 90-91 -0.4628448532453362 1103 92-93 -0.3114707230824685 1103 94-95 -0.5342913291505589 1103 96-97 -0.7621788539542642 1103 98-99 -0.4436893003797344 1103 100-101 -0.3722774102116375 1104 1 0.19485303666521503 1104 2 0.28573423330033165 1104 3 0.24163777350350912 1104 4 0.16953686342683483 1104 5 0.1708071813467953 1104 6 0.19670597643884946 1104 7 0.06918936539629073 1104 8 0.1863924348254642 1104 9 0.21051559955270704 1104 10-11 0.2180486907914272 1104 12-13 0.20512375388638304 1104 14-15 0.2847275893373009 1104 16-17 0.288113257414814 1104 18-19 0.19382445030913686 1104 20-21 0.22720744705686968 1104 22-23 0.25353624653197215 1104 24-25 0.18051713744467435 1104 26-27 0.29333519352331194 1104 28-29 0.3199455027282383 1104 30-31 0.1559194926566292 1104 32-33 0.1785028292475772 1104 34-35 0.2080333320902099 1104 36-37 0.2734365526719884 1104 38-39 0.3909582538731726 1104 40-41 0.21602436617624932 1104 42-43 0.17532746684430123 1104 44-45 0.26573074914055184 1104 46-47 0.289814730969006 1104 48-49 0.41438368614652177 1104 50-51 0.2838823032455977 1104 52-53 0.20502705041220537 1104 54-55 0.3885288446318782 1104 56-57 0.23835345547007591 1104 58-59 0.2078729751867101 1104 60-61 0.18832626043252532 1104 62-63 0.30502607368660506 1104 64-65 0.41116095748697035 1104 66-67 0.4449383858441216 1104 68-69 -3.782301002424955 1104 70-71 0.8988818773078897 1104 72-73 1.0363133220166567 1104 74-75 0.7693770935833228 1104 76-77 0.5587225801359104 1104 78-79 0.20393121114575408 1104 80-81 0.12772118997978765 1104 82-83 0.1933219361168561 1104 84-85 0.14873733283956625 1104 86-87 0.20435258852357308 1104 88-89 -0.018273024928291193 1104 90-91 -0.1416860705112981 1104 92-93 -0.3185334709259884 1104 94-95 0.09906583498024091 1104 96-97 -0.2464001369837696 1104 98-99 -0.06819619197131033 1104 100-101 -0.006770458881902641 1105 1 0.12480870524966647 1105 2 0.17469624685585927 1105 3 0.1066254845889425 1105 4 0.0017892090411422146 1105 5 0.18298740155461246 1105 6 0.19671193229405048 1105 7 0.1135436117225197 1105 8 0.13788447581762853 1105 9 0.13543757784957222 1105 10-11 0.13086588971301438 1105 12-13 0.13737044143824306 1105 14-15 0.13698366728753797 1105 16-17 0.25670133260152994 1105 18-19 0.16790233502760543 1105 20-21 0.21756863989555342 1105 22-23 0.19134073962913334 1105 24-25 0.15599985950873219 1105 26-27 0.18557739433092735 1105 28-29 0.19328803785785453 1105 30-31 0.23823958612384644 1105 32-33 0.12936156430335188 1105 34-35 0.15721896815569636 1105 36-37 0.1359635029167734 1105 38-39 0.43011700917885065 1105 40-41 0.2336941477551946 1105 42-43 0.10737215164059677 1105 44-45 0.1107792496547404 1105 46-47 0.07872334035622686 1105 48-49 0.17806304474061818 1105 50-51 0.1664715517357891 1105 52-53 0.1786815730901239 1105 54-55 0.1946660059918841 1105 56-57 0.1156084911456503 1105 58-59 0.1975075536908406 1105 60-61 0.17024875093293446 1105 62-63 0.11322098301864258 1105 64-65 0.1291827301083046 1105 66-67 0.7704290986805837 1105 68-69 1.9886879276255094 1105 70-71 2.2979385691082648 1105 72-73 1.3371542391389468 1105 74-75 0.7780733866590452 1105 76-77 0.4368561044536676 1105 78-79 0.4223460702103665 1105 80-81 0.3725696233913425 1105 82-83 0.3573764639565091 1105 84-85 0.24847135427270572 1105 86-87 0.21248530880087557 1105 88-89 0.3152412175067809 1105 90-91 0.29209747584312495 1105 92-93 0.44691676194794994 1105 94-95 0.5639081735666416 1105 96-97 0.1103357367203337 1105 98-99 0.16835871307250372 1105 100-101 0.36517369107858855 1106 1 0.35678694430648505 1106 2 0.3118719552563256 1106 3 0.3615374953174566 1106 4 0.24953882553526796 1106 5 0.46577896249586814 1106 6 0.4787431144569254 1106 7 0.362782629947489 1106 8 0.4097111456359599 1106 9 0.5521251484001795 1106 10-11 0.45962509144192865 1106 12-13 0.5187781853624571 1106 14-15 0.6105094007726635 1106 16-17 0.5947659786666151 1106 18-19 0.5497581054643632 1106 20-21 0.5434343621890605 1106 22-23 0.5175661494256545 1106 24-25 0.5933117245532529 1106 26-27 0.6724754830727377 1106 28-29 0.6869342621340166 1106 30-31 0.739140764620501 1106 32-33 0.8156513500224563 1106 34-35 0.7843029245855675 1106 36-37 0.8397483320034027 1106 38-39 0.8345577177240315 1106 40-41 0.678198556866711 1106 42-43 0.7478694670994983 1106 44-45 0.7161064838779367 1106 46-47 0.7467950897218643 1106 48-49 0.7892206535935813 1106 50-51 0.8250464053282016 1106 52-53 0.8279199280187797 1106 54-55 0.7432572852130974 1106 56-57 0.8212267315531747 1106 58-59 0.9334625556686618 1106 60-61 1.062502897137911 1106 62-63 0.9766464622803781 1106 64-65 1.0388857904369146 1106 66-67 1.4127501775139528 1106 68-69 2.987247067125171 1106 70-71 3.2969158867861985 1106 72-73 2.224253366964586 1106 74-75 1.6691691762952203 1106 76-77 1.200117508326354 1106 78-79 1.1272440311168914 1106 80-81 1.0858136609604436 1106 82-83 1.0321942383682234 1106 84-85 1.104924986242139 1106 86-87 1.1730668146523549 1106 88-89 1.379817334980178 1106 90-91 1.346575811379406 1106 92-93 1.265849530233222 1106 94-95 1.6415159531882466 1106 96-97 1.4957612473378994 1106 98-99 1.7181056353775936 1106 100-101 1.9276664551187643 1107 1 0.11687671467791105 1107 2 0.20483911604502225 1107 3 0.19552621901206635 1107 4 0.1535736685033129 1107 5 0.21363772138403903 1107 6 0.18329033969208552 1107 7 0.16590790004683953 1107 8 0.1576027729518117 1107 9 0.14859565750777648 1107 10-11 0.14684631848120233 1107 12-13 0.1450584943134956 1107 14-15 0.2501536928658581 1107 16-17 0.25713525058225173 1107 18-19 0.241158682427006 1107 20-21 0.22492366260284058 1107 22-23 0.2014390575632774 1107 24-25 0.2234461229453828 1107 26-27 0.2608241428321705 1107 28-29 0.2543515158673273 1107 30-31 0.3247895341239584 1107 32-33 0.2436714715869499 1107 34-35 0.27383022181475525 1107 36-37 0.2307157304795311 1107 38-39 0.3020537339488598 1107 40-41 0.28699076997099127 1107 42-43 0.21142914672971358 1107 44-45 0.25530079045758924 1107 46-47 0.24513101144388116 1107 48-49 0.15259991446784937 1107 50-51 0.281151380999475 1107 52-53 0.2714544419982019 1107 54-55 0.2423786634471341 1107 56-57 0.17883377915274679 1107 58-59 0.41291834276403705 1107 60-61 0.5746698456209103 1107 62-63 0.46902494344250556 1107 64-65 0.46513086473782295 1107 66-67 1.0719082344785882 1107 68-69 2.389612581996918 1107 70-71 2.671430773140564 1107 72-73 1.5676640689246426 1107 74-75 0.9215888099461615 1107 76-77 0.7353547833754455 1107 78-79 0.8639945168392096 1107 80-81 0.8971664234909227 1107 82-83 0.8478001526157151 1107 84-85 0.785060251995553 1107 86-87 0.8327044311070217 1107 88-89 0.8811277352811047 1107 90-91 0.7502523732688289 1107 92-93 0.7882234950299178 1107 94-95 1.2165075154367884 1107 96-97 0.8124747591224484 1107 98-99 1.0703090602068883 1107 100-101 1.0971323401255262 1108 1 -0.1439574446026164 1108 2 -0.15941542311984946 1108 3 -0.1415929075665936 1108 4 -0.06699246358906663 1108 5 -0.12942685050548874 1108 6 -0.009190621926741471 1108 7 -0.04318011608334871 1108 8 -0.050117936161200305 1108 9 -0.1124095146180224 1108 10-11 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2316 64-65 -0.35001639370769766 2316 66-67 0.19730628574305697 2316 68-69 1.550161840751965 2316 70-71 1.7523930923770763 2316 72-73 0.8562935116824235 2316 74-75 0.3094310048796949 2316 76-77 0.0038193600691194263 2316 78-79 -0.2327976939977674 2316 80-81 -0.2697434465605397 2316 82-83 -0.0838935170694306 2316 84-85 -0.30521099967879906 2316 86-87 -0.236171818210817 2316 88-89 -0.1932750975239017 2316 90-91 -0.2055195260357756 2316 92-93 -0.20720456591233827 2316 94-95 0.02660540315737947 2316 96-97 0.015991116684400453 2316 98-99 -0.15247344920109995 2316 100-101 -0.29149747477064736 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 24.0 4 56.0 5 158.0 6 335.0 7 894.0 8 2056.0 9 3429.0 10 5681.0 11 8448.0 12 11846.0 13 15096.0 14 18300.0 15 22479.0 16 26940.0 17 31477.0 18 30348.0 19 27557.0 20 27800.0 21 29525.0 22 31925.0 23 36600.0 24 43371.0 25 52792.0 26 65513.0 27 81345.0 28 99765.0 29 119739.0 30 144056.0 31 171591.0 32 204090.0 33 248002.0 34 307316.0 35 391095.0 36 495242.0 37 545382.0 38 309714.0 39 23505.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.25947342892237 19.449468347210317 14.118983635565819 24.17207458830149 2 15.750926943705082 21.28351938275143 38.34010940261854 24.62544427092495 3 19.090952625240266 24.75533205485846 30.36075999533232 25.792955324568958 4 13.288788575420002 16.915980986921973 36.825797942974525 32.969432494683495 5 13.592325193979688 37.54771915445691 34.538250870579574 14.32170478098384 6 32.14013052986406 36.500639328823084 17.079849775615674 14.27938036569719 7 28.037492235445775 32.66793248050983 21.33271537985837 17.96185990418602 8 26.1483490156458 34.8279440836505 20.038893639436807 18.984813261266893 9 26.12864349212701 16.237103684354942 20.235838787901137 37.39841403561691 10-11 24.94272050164318 26.105263824209118 27.844303796423226 21.107711877724476 12-13 25.82480413507751 23.508524427831134 28.10604874254816 22.560622694543195 14-15 22.527046243854755 25.042472834036833 26.14047781491064 26.290003107197773 16-17 22.418715467912158 28.25532721808654 26.729478697664078 22.59647861633723 18-19 22.399541378646504 27.17997544240076 28.46423357543701 21.95624960351573 20-21 22.965384055424263 26.30157597401293 28.24054719706817 22.49249277349464 22-23 22.699964964900225 26.2851730522012 28.233212669110408 22.78164931378817 24-25 22.812290362109785 26.514301991400846 28.160938599792622 22.512469046696744 26-27 22.593226153536428 26.504892886025956 28.379110494195732 22.522770466241887 28-29 22.373215441379948 27.0205434352283 28.198031840680184 22.40820928271157 30-31 22.608912715430346 26.60553248769844 28.046766697408522 22.738788099462692 32-33 22.36427225295159 26.665547428079172 28.196817188202033 22.773363130767205 34-35 23.063476054375766 26.54666985381108 27.93386207453652 22.455992017276635 36-37 22.947746699352187 26.263863933028848 28.13153111157763 22.65685825604134 38-39 22.547627223442824 26.52394649591375 28.2333075898189 22.695118690824522 40-41 22.64634036643929 26.428373361045338 28.305835073254382 22.61945119926099 42-43 22.538279682406127 26.519664200600218 27.927190014951787 23.014866102041868 44-45 22.59733884162281 26.39967845047953 27.986943524697356 23.016039183200306 46-47 22.719084381829504 26.699019698721226 27.724098624316646 22.857797295132624 48-49 22.616220924538954 26.657947087230273 27.718510929799073 23.007321058431696 50-51 22.183960986854302 26.756003383871956 27.979502837477455 23.080532791796287 52-53 22.575220346881434 26.86991202649726 27.48717523588037 23.06769239074094 54-55 22.16643369045337 26.50881013595236 27.763122674368653 23.561633499225614 56-57 22.251007286047212 26.65777878112538 27.64722481853709 23.443989114290314 58-59 22.49209333307643 26.406258171598758 27.616659464190853 23.484989031133964 60-61 22.219682361081826 26.530462828451757 27.929388068090176 23.32046674237624 62-63 22.012183785841252 26.524222048251577 27.783223343306908 23.680370822600263 64-65 21.83928166202286 26.714958434153342 27.50364231835163 23.942117585472175 66-67 21.635672741415377 26.58944990985314 27.6151064135333 24.15977093519818 68-69 21.44991675244734 26.450863218398744 27.90105631126985 24.19816371788406 70-71 21.593576766326404 26.531923244241945 27.61681580007839 24.257684189353267 72-73 21.66739592733421 26.36768082149215 27.551991874188886 24.412931376984755 74-75 21.888658310033797 26.93366431707486 26.776234141356525 24.40144323153482 76-77 21.960528338880856 26.664782698287787 26.844856305198473 24.529832657632884 78-79 22.067088924283446 26.72317960921216 26.758023706646828 24.451707759857563 80-81 22.198128227816223 26.745714478368388 26.567330543518498 24.488826750296894 82-83 22.041679945881352 26.67572680650987 26.6904921923722 24.59210105523658 84-85 21.961737216829547 26.624170199438364 26.526825464909177 24.887267118822916 86-87 22.08841779750067 26.600281715414585 26.570709303048833 24.740591184035907 88-89 22.00767498699254 26.535950338675622 26.33173904493791 25.124635629393932 90-91 22.06055133385937 26.43540804831953 26.34618226773844 25.157858350082662 92-93 22.11123563406618 26.362558491691857 26.462670727484998 25.063535146756973 94-95 21.799147945108892 26.04713844404685 26.75969055446365 25.394023056380604 96-97 21.907250299935573 25.93535375056068 26.749628770802975 25.40776717870077 98-99 21.927981600047676 26.123354608266737 26.320872497529823 25.627791294155767 100-101 21.708516765981432 25.871754929715863 26.570350678220844 25.84937762608186 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 600.0 1 440.5 2 347.0 3 480.0 4 478.0 5 466.0 6 751.5 7 1492.0 8 2295.0 9 2614.5 10 2451.5 11 2295.5 12 2409.0 13 2613.0 14 2847.0 15 3039.0 16 3239.0 17 3528.0 18 3879.5 19 4322.5 20 4976.0 21 5926.5 22 7174.0 23 8558.5 24 10261.0 25 12870.5 26 16051.5 27 20343.0 28 25096.5 29 30271.0 30 36184.0 31 42417.0 32 49084.0 33 53714.5 34 57681.5 35 63968.0 36 71748.0 37 79059.5 38 86198.5 39 91597.0 40 96277.0 41 103551.0 42 110708.0 43 117839.5 44 125318.5 45 133172.5 46 139893.0 47 148185.0 48 160318.5 49 160588.5 50 153522.5 51 149974.0 52 144844.5 53 141408.0 54 138063.0 55 128345.0 56 114989.0 57 102610.0 58 91068.0 59 78120.5 60 64425.5 61 52349.0 62 42099.5 63 32810.0 64 24390.0 65 17829.5 66 12954.5 67 10015.5 68 7229.0 69 4614.0 70 3236.0 71 2424.5 72 1905.5 73 1510.0 74 1208.0 75 921.0 76 575.5 77 291.0 78 157.0 79 102.0 80 68.5 81 40.0 82 20.0 83 15.0 84 10.5 85 9.5 86 11.0 87 6.0 88 3.0 89 1.5 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023806253971722573 2 0.02372368892904608 3 8.256504267649447E-5 4 0.0 5 0.0 6 2.7521680892164824E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.440210111520603E-4 18-19 1.3760840446082412E-5 20-21 0.0 22-23 0.0 24-25 4.128252133824723E-5 26-27 2.614559684755658E-4 28-29 6.880420223041206E-5 30-31 4.403468942746372E-4 32-33 0.0021054085882506093 34-35 0.004197056336055136 36-37 0.10339895511186324 38-39 0.09518373336555205 40-41 0.10415580133639778 42-43 0.10546308117877562 44-45 0.09841753087038141 46-47 0.09190865333938443 48-49 0.0923214785527669 50-51 0.09186737081804618 52-53 0.07246458578906999 54-55 0.06129078334685107 56-57 0.033411320603088095 58-59 0.013458101956268599 60-61 0.008724372842816248 62-63 0.0017476267366524664 64-65 0.004609881549437608 66-67 0.15219489533367148 68-69 0.40660531350084317 70-71 0.3908216295091866 72-73 0.17993674967297363 74-75 0.030136240576920482 76-77 0.030150001417366568 78-79 0.00473372911345235 80-81 0.0012109539592552523 82-83 2.7521680892164824E-5 84-85 5.64194458289379E-4 86-87 0.001238475640147417 88-89 2.0641260669123618E-4 90-91 5.504336178432965E-4 92-93 0.0021191694286966916 94-95 0.07560205741077677 96-97 0.017490028206970747 98-99 0.01826063527195136 100-101 0.009728914195380266 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3633499.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.842701325920864 #Duplication Level Percentage of deduplicated Percentage of total 1 63.07818980185331 23.87049096851282 2 14.73344757784447 11.15106912378961 3 7.0825996183630595 8.04074105906383 4 4.081363973752073 6.177993514442928 5 2.6182818098367364 4.954142825837157 6 1.8698093889046905 4.245518294443369 7 1.2876235620089054 3.410900771412494 8 0.9368131657244877 2.836123266296175 9 0.6938073081946996 2.3629988467577827 >10 3.402467107969996 21.890891825541765 >50 0.14511325829294075 3.749003712828083 >100 0.06466636007958104 4.247352883576513 >500 0.003635666993565992 0.9569755138514624 >1k 0.0018905468239519168 1.2662906420943612 >5k 2.908533575310641E-4 0.8395067515516823 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9606 0.2643732666501353 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8614 0.23707175920510778 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6994 0.1924866361598008 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5295 0.14572730032401277 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4762 0.1310582444084889 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3894 0.10716942539408983 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.880420223041206E-5 0.0 12-13 0.0 0.0 0.0 1.100867235686593E-4 0.0 14-15 0.0 0.0 0.0 1.3760840446082413E-4 0.0 16-17 0.0 0.0 0.0 1.9265176624515376E-4 0.0 18-19 0.0 0.0 0.0 2.201734471373186E-4 0.0 20-21 0.0 0.0 0.0 3.440210111520603E-4 0.0 22-23 0.0 0.0 0.0 8.394112672110272E-4 0.0 24-25 0.0 0.0 0.0 0.0019127568220054553 0.0 26-27 0.0 0.0 0.0 0.003866796165349158 0.0 28-29 0.0 0.0 0.0 0.013416819434930352 0.0 30-31 0.0 0.0 0.0 0.04465392724753743 0.0 32-33 0.0 0.0 0.0 0.11576995067289134 0.0 34-35 0.0 0.0 0.0 0.2247833286867562 0.0 36-37 0.0 0.0 0.0 0.3978671798175808 0.0 38-39 0.0 0.0 0.0 0.6626119891597604 0.0 40-41 0.0 0.0 0.0 0.9631487445022002 0.0 42-43 0.0 0.0 0.0 1.2603416156162421 0.0 44-45 0.0 0.0 0.0 1.6086972915088182 0.0 46-47 0.0 0.0 0.0 2.018027251417986 0.0 48-49 0.0 0.0 0.0 2.4369209954371804 0.0 50-51 0.0 0.0 0.0 2.8815612719309955 0.0 52-53 0.0 0.0 0.0 3.311793948477762 0.0 54-55 0.0 0.0 0.0 3.7677318749778106 0.0 56-57 0.0 0.0 0.0 4.282235938416386 1.3760840446082412E-5 58-59 0.0 0.0 0.0 4.7993270398588255 2.7521680892164824E-5 60-61 0.0 0.0 0.0 5.316308054577695 2.7521680892164824E-5 62-63 0.0 0.0 0.0 5.824014262835906 2.7521680892164824E-5 64-65 0.0 0.0 0.0 6.347063808191498 2.7521680892164824E-5 66-67 0.0 0.0 0.0 6.897235970066319 2.7521680892164824E-5 68-69 0.0 0.0 0.0 7.495860051151796 2.7521680892164824E-5 70-71 0.0 0.0 0.0 8.15494926515736 2.7521680892164824E-5 72-73 0.0 0.0 0.0 8.827248885991162 2.7521680892164824E-5 74-75 0.0 0.0 0.0 9.517767859575578 2.7521680892164824E-5 76-77 0.0 0.0 0.0 10.239097354918771 2.7521680892164824E-5 78-79 0.0 0.0 0.0 11.034845475394379 2.7521680892164824E-5 80-81 0.0 0.0 0.0 11.856835518600665 2.7521680892164824E-5 82-83 0.0 0.0 0.0 12.677284347676991 2.7521680892164824E-5 84-85 0.0 0.0 0.0 13.476101135572076 2.7521680892164824E-5 86-87 0.0 0.0 0.0 14.281990995456447 2.7521680892164824E-5 88-89 0.0 0.0 0.0 15.122406804020038 2.7521680892164824E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11560 0.0 24.42651 1 TACATGG 12130 0.0 22.570107 2 ACATGGG 11965 0.0 21.607315 3 GAGTACT 8695 0.0 21.140686 12-13 GTATCAA 20910 0.0 20.380957 1 AGTACTT 9450 0.0 19.978746 12-13 TACCGGA 270 2.3319444E-8 19.330698 2 GTACTTT 9695 0.0 18.960112 14-15 AGAGTAC 15975 0.0 18.440289 10-11 CATGGGG 6445 0.0 18.17988 4 TCAACGC 23455 0.0 18.080858 4 ATCAACG 23405 0.0 18.058681 3 CAACGCA 23505 0.0 17.981853 5 ATTCGCG 465 0.0 17.854345 80-81 GAGTACA 10320 0.0 17.796457 1 AACGCAG 23800 0.0 17.7789 6 TATCAAC 23985 0.0 17.725027 2 CGCGCTT 560 0.0 17.48224 62-63 AGTACAT 9495 0.0 17.29019 2 GGTATCA 16820 0.0 17.126356 1 >>END_MODULE