##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939776_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 236836 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.122865611646876 31.0 31.0 33.0 30.0 34.0 2 31.253812764951274 31.0 31.0 34.0 28.0 34.0 3 31.239566619939538 31.0 31.0 34.0 28.0 34.0 4 35.041146616223884 35.0 35.0 37.0 32.0 37.0 5 34.8342777280481 35.0 35.0 37.0 32.0 37.0 6 34.94505480585722 35.0 35.0 37.0 32.0 37.0 7 34.843495076761975 35.0 35.0 37.0 32.0 37.0 8 34.90729027681603 36.0 35.0 37.0 32.0 37.0 9 36.25470789913695 38.0 35.0 39.0 32.0 39.0 10-11 36.157020047627896 38.0 35.0 39.0 31.0 39.0 12-13 36.11850183249168 38.0 35.0 39.0 31.0 39.0 14-15 36.69577471330372 38.0 35.5 40.0 30.5 41.0 16-17 36.68636313736087 38.0 35.5 40.0 30.5 41.0 18-19 36.79223597763853 38.0 35.5 40.0 31.0 41.0 20-21 36.71302082453681 38.0 35.0 40.0 31.0 41.0 22-23 36.49986699657147 38.0 35.0 40.0 30.5 41.0 24-25 36.11527808272391 38.0 34.5 40.0 30.0 41.0 26-27 35.54912048843926 37.5 34.0 40.0 28.5 41.0 28-29 35.278532402168594 37.0 34.0 40.0 29.0 41.0 30-31 34.73298611697546 36.5 33.0 39.0 27.0 41.0 32-33 34.15546411863062 35.5 33.0 39.0 25.0 41.0 34-35 33.58650711885018 35.0 33.0 39.0 24.0 41.0 36-37 33.14549730615278 35.0 33.0 39.0 21.5 41.0 38-39 32.57658041851745 35.0 33.0 39.0 18.5 41.0 40-41 32.380161799726395 35.0 33.0 39.0 15.5 41.0 42-43 31.765580401628128 35.0 31.5 39.0 5.5 41.0 44-45 31.111499096421152 35.0 31.0 39.0 2.0 41.0 46-47 30.389218699859818 35.0 29.5 39.0 2.0 41.0 48-49 29.717944062558054 35.0 27.5 39.0 2.0 41.0 50-51 29.137409430998666 35.0 26.0 39.0 2.0 40.0 52-53 28.841035568916887 35.0 25.5 39.0 2.0 40.0 54-55 28.40406652704825 35.0 23.5 39.0 2.0 40.0 56-57 27.59149580300292 34.5 21.0 38.0 2.0 40.0 58-59 26.960136972419733 34.0 17.0 38.0 2.0 40.0 60-61 26.088711175665864 33.0 7.0 38.0 2.0 40.0 62-63 25.54034226215609 33.0 2.0 37.0 2.0 40.0 64-65 24.87602180411762 33.0 2.0 36.5 2.0 39.5 66-67 23.64506873954973 31.5 2.0 36.0 2.0 39.0 68-69 21.998000726240942 29.0 2.0 35.0 2.0 38.0 70-71 21.245765001942274 28.5 2.0 35.0 2.0 37.0 72-73 21.533514752824736 29.0 2.0 35.0 2.0 36.0 74-75 21.56626737489233 29.0 2.0 34.5 2.0 36.0 76-77 21.08575765508622 29.0 2.0 34.0 2.0 35.0 78-79 21.385849279670317 30.0 2.0 34.0 2.0 35.0 80-81 21.142372781165026 29.0 2.0 34.0 2.0 35.0 82-83 20.743740394196827 29.0 2.0 34.0 2.0 35.0 84-85 20.458914607576553 29.0 2.0 34.0 2.0 35.0 86-87 20.24957354456248 29.0 2.0 34.0 2.0 35.0 88-89 19.800207738688375 28.0 2.0 34.0 2.0 35.0 90-91 19.387240537756085 27.0 2.0 34.0 2.0 35.0 92-93 19.005265246837475 25.5 2.0 34.0 2.0 35.0 94-95 18.37892254555895 25.0 2.0 33.5 2.0 35.0 96-97 17.898594385988616 24.0 2.0 33.0 2.0 35.0 98-99 17.380375027445155 21.0 2.0 33.0 2.0 35.0 100-101 16.305038085426204 9.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.39649636434929647 1101 2 0.49057760340451395 1101 3 0.555342772352514 1101 4 0.6275810790649459 1101 5 0.5212876951295442 1101 6 0.44779464307100625 1101 7 0.46018248123978367 1101 8 0.5655978656537997 1101 9 0.7761209425200093 1101 10-11 0.8988904603803505 1101 12-13 0.567188893497061 1101 14-15 0.608253174950633 1101 16-17 0.4027951497019657 1101 18-19 0.43176362574499194 1101 20-21 0.3665846233304819 1101 22-23 0.34727577939098353 1101 24-25 0.6564932430548822 1101 26-27 0.8142945052377399 1101 28-29 0.6022770419708294 1101 30-31 0.7257996842304522 1101 32-33 0.7601760418137147 1101 34-35 1.475356170616621 1101 36-37 1.2444715984262231 1101 38-39 1.0687837254991521 1101 40-41 1.1504302099665367 1101 42-43 0.5857639741070422 1101 44-45 0.2922436015302168 1101 46-47 -0.2630603611649498 1101 48-49 -0.09144889532531764 1101 50-51 -0.6043059092808925 1101 52-53 -0.37730371643857197 1101 54-55 -0.8078613267635077 1101 56-57 -0.4438616170976104 1101 58-59 -0.2440555871961756 1101 60-61 -0.8295527647349736 1101 62-63 -1.0447219810858464 1101 64-65 -1.6911077539950021 1101 66-67 -1.1072338768005778 1101 68-69 -6.7351757923013285 1101 70-71 -5.125313797608413 1101 72-73 -2.2323469730793306 1101 74-75 -1.2379374976581268 1101 76-77 -1.1984629536171134 1101 78-79 -1.5000590137743153 1101 80-81 -1.2981919808167213 1101 82-83 -1.1339353660151623 1101 84-85 -0.7621901027235118 1101 86-87 -0.7269821650843298 1101 88-89 -0.7359482543625973 1101 90-91 -0.8142450301395066 1101 92-93 -0.4545528975110287 1101 94-95 -0.312800950378481 1101 96-97 -0.9635227374260431 1101 98-99 -0.6464654136703523 1101 100-101 -1.3421523141299794 1102 1 0.2742404665773428 1102 2 0.02969129934160364 1102 3 -0.03164936395155138 1102 4 0.3987966753952179 1102 5 0.44445283274422565 1102 6 0.47310591436851723 1102 7 0.2540471601388674 1102 8 0.3317757817743754 1102 9 0.4153329346563126 1102 10-11 0.3481040907866415 1102 12-13 0.010914319447266507 1102 14-15 0.11716126800437365 1102 16-17 0.4203655953114094 1102 18-19 0.33369268865455126 1102 20-21 0.2782204359123881 1102 22-23 0.16481345960068694 1102 24-25 0.02655795471935818 1102 26-27 -0.08351840432975877 1102 28-29 0.251441524277503 1102 30-31 -0.04783236033049576 1102 32-33 -0.17132379435928868 1102 34-35 -0.049156444062276705 1102 36-37 0.4771264149399812 1102 38-39 1.0081063008595734 1102 40-41 1.2273879426008776 1102 42-43 0.7457205599523853 1102 44-45 0.3906217142431885 1102 46-47 0.42695847893989836 1102 48-49 0.4138959605069239 1102 50-51 0.4018785599196306 1102 52-53 0.6923841931289232 1102 54-55 0.7974384766440927 1102 56-57 0.7931550932562565 1102 58-59 0.7227284888195484 1102 60-61 0.9401679102322618 1102 62-63 0.785389421273262 1102 64-65 1.0846540415489052 1102 66-67 0.8430651074720288 1102 68-69 -2.5088692719867787 1102 70-71 1.0294822377782182 1102 72-73 1.8215930662391457 1102 74-75 1.8453070960509201 1102 76-77 1.9445989729883912 1102 78-79 1.5490276507079876 1102 80-81 1.5716269903497277 1102 82-83 1.811571678545782 1102 84-85 1.4763215289933989 1102 86-87 1.7853048598173764 1102 88-89 0.6720014507488088 1102 90-91 0.557437473137032 1102 92-93 0.09330506448110754 1102 94-95 0.3597703143659494 1102 96-97 0.37490288511655123 1102 98-99 0.7019741341671342 1102 100-101 -0.07148963392028307 1103 1 0.23846065006357975 1103 2 0.002779984356894971 1103 3 0.32050150251124165 1103 4 0.34008107906494445 1103 5 0.18628769512954335 1103 6 0.08592956370593186 1103 7 0.40087692568422995 1103 8 -0.027755308949373614 1103 9 0.5936407837898514 1103 10-11 0.720795222285112 1103 12-13 0.6640539728621491 1103 14-15 0.7090369051093646 1103 16-17 0.42884673700355336 1103 18-19 0.5683410066973664 1103 20-21 0.15492787729873214 1103 22-23 0.18785117621637681 1103 24-25 0.9308087192453485 1103 26-27 0.40594133063456894 1103 28-29 0.05818974038351854 1103 30-31 1.0166528588336305 1103 32-33 1.19355897832164 1103 34-35 1.9148700595055104 1103 36-37 1.975791042870668 1103 38-39 1.7678412651816942 1103 40-41 1.7587437020300314 1103 42-43 1.9862302439483095 1103 44-45 2.3656166174032336 1103 46-47 2.8318106705810777 1103 48-49 2.5054657872143657 1103 50-51 2.91099170976673 1103 52-53 3.202924458164599 1103 54-55 2.6273569272047474 1103 56-57 2.502309017823027 1103 58-59 2.5083452064546172 1103 60-61 2.1739293781221676 1103 62-63 1.842341510977647 1103 64-65 2.5773247856875408 1103 66-67 2.9041252501835473 1103 68-69 -1.1598682526187893 1103 70-71 2.5882080277884114 1103 72-73 3.011164931682572 1103 74-75 2.6986200420244124 1103 76-77 2.140287046382884 1103 78-79 1.6564588433685365 1103 80-81 2.000607622357883 1103 82-83 1.7326420149372197 1103 84-85 1.6863317226733123 1103 86-87 1.6132559301537697 1103 88-89 0.8729902377008969 1103 90-91 0.4879573508128736 1103 92-93 1.2010522612191323 1103 94-95 0.9648875416850125 1103 96-97 1.0881240879707832 1103 98-99 1.634675459345523 1103 100-101 1.333770304917639 1104 1 -0.0174345464429706 1104 2 -0.0018779486853155447 1104 3 0.2575417742240056 1104 4 0.127854004829139 1104 5 0.2816588741688477 1104 6 0.3119945806879798 1104 7 0.16010060351052857 1104 8 0.24453969347663218 1104 9 0.6132817347845148 1104 10-11 0.41043132126619497 1104 12-13 0.43388415238664635 1104 14-15 0.1928894038963591 1104 16-17 0.2469350436508151 1104 18-19 0.6900586195066865 1104 20-21 0.40757222470290344 1104 22-23 0.5029409384677095 1104 24-25 0.4844622854753382 1104 26-27 0.8496773810955105 1104 28-29 0.5712804730375751 1104 30-31 1.1764975943988887 1104 32-33 0.26260313164527105 1104 34-35 0.5646711269484967 1104 36-37 0.07082258095897487 1104 38-39 0.36546377852473455 1104 40-41 0.20000522556229328 1104 42-43 0.49198473206088167 1104 44-45 0.2465870201203586 1104 46-47 0.30562920527297877 1104 48-49 0.12483502544823111 1104 50-51 0.14770204455566116 1104 52-53 -0.029021199283238985 1104 54-55 0.4612239820324966 1104 56-57 0.3026098426653334 1104 58-59 0.01337189252933868 1104 60-61 -0.11593961751101745 1104 62-63 0.3677078380033656 1104 64-65 0.3437682597292664 1104 66-67 0.2826308299991709 1104 68-69 -2.7516780536862306 1104 70-71 0.5852191874196606 1104 72-73 0.5425477555544802 1104 74-75 0.38925565580413135 1104 76-77 0.26482853109904525 1104 78-79 -0.33375949724281284 1104 80-81 0.06708991250829754 1104 82-83 0.7630059159561426 1104 84-85 0.6595874237892794 1104 86-87 0.5383796565002008 1104 88-89 0.35381508001045603 1104 90-91 0.5022513828361639 1104 92-93 0.580625284023597 1104 94-95 1.1649263577936964 1104 96-97 0.7290653786063963 1104 98-99 0.7128511022373232 1104 100-101 0.4915286746410743 1105 1 0.09693522633476803 1105 2 0.03696077531734687 1105 3 -0.033942941933201354 1105 4 0.2070302316073196 1105 5 0.12510125445157882 1105 6 0.057812802877307945 1105 7 -0.06577090859072143 1105 8 0.05265900366833165 1105 9 0.20961065196311068 1105 10-11 -0.022114382234654784 1105 12-13 0.024967343860261337 1105 14-15 -0.1751533020469438 1105 16-17 -0.24830957184767044 1105 18-19 -0.12646125318963897 1105 20-21 -0.07821713211746584 1105 22-23 -0.11224298574219205 1105 24-25 0.28849233506456073 1105 26-27 -0.4655935092901089 1105 28-29 -0.21143361178220488 1105 30-31 -0.41910043683492404 1105 32-33 -0.4066293455954906 1105 34-35 -0.1785103548071092 1105 36-37 -0.5561110286924205 1105 38-39 -0.24072747304806086 1105 40-41 0.050626941201400655 1105 42-43 -0.9851727692343601 1105 44-45 -1.1479803694140962 1105 46-47 -0.8619934725450946 1105 48-49 -0.6161537984730181 1105 50-51 0.11731939338254449 1105 52-53 0.453696586224865 1105 54-55 0.14166954490719164 1105 56-57 0.14580242648592545 1105 58-59 0.6329873910120547 1105 60-61 0.7399463272747084 1105 62-63 0.6335467840473257 1105 64-65 -0.12217615593204556 1105 66-67 0.28543410140765246 1105 68-69 1.3636816532192526 1105 70-71 1.1289613234569629 1105 72-73 1.0302892254678824 1105 74-75 0.5468309648116083 1105 76-77 0.003337893840512862 1105 78-79 0.08510291116514779 1105 80-81 0.07366788601136776 1105 82-83 0.3353821279315703 1105 84-85 0.4009833234266118 1105 86-87 0.6316740092498137 1105 88-89 0.6968226294146405 1105 90-91 0.5541720400784129 1105 92-93 0.2769467998255344 1105 94-95 0.40227925543265997 1105 96-97 -0.004003972292871083 1105 98-99 0.04455304879938282 1105 100-101 -0.5349867572292535 1106 1 0.20409941658340358 1106 2 0.21188424280036244 1106 3 0.12134340167913393 1106 4 0.27488284117948325 1106 5 0.22558284931456285 1106 6 0.11185222645679005 1106 7 0.18251177639396587 1106 8 0.3827194830232159 1106 9 0.07050813572327996 1106 10-11 0.3340698184671993 1106 12-13 0.5248273453535788 1106 14-15 0.6282047954666794 1106 16-17 0.3260763013696817 1106 18-19 0.27841442813642914 1106 20-21 0.1446978234563403 1106 22-23 0.30933918404799954 1106 24-25 -0.1291325436033972 1106 26-27 -0.2369759478774256 1106 28-29 -0.22926008829978883 1106 30-31 -0.4005179683812585 1106 32-33 -0.6031125831076238 1106 34-35 -0.19569283819394911 1106 36-37 0.049055849527547934 1106 38-39 0.2862455725727884 1106 40-41 -0.029694868699287724 1106 42-43 0.02767909367910093 1106 44-45 -0.09530359796632126 1106 46-47 0.23049887420320658 1106 48-49 0.3355000505525929 1106 50-51 0.3156319447153315 1106 52-53 0.2841677278660235 1106 54-55 0.18893304076323858 1106 56-57 0.466557671763308 1106 58-59 0.801629592161909 1106 60-61 0.8393919331882458 1106 62-63 0.37716285214260026 1106 64-65 -0.009645356260573834 1106 66-67 0.11817321885706633 1106 68-69 1.0636461869308924 1106 70-71 1.7034405290121057 1106 72-73 0.888228074120164 1106 74-75 0.5579935124110982 1106 76-77 -0.01495524436601059 1106 78-79 -0.4421314650015056 1106 80-81 -0.08369976873364848 1106 82-83 -0.0967559292624891 1106 84-85 -0.6751758642087218 1106 86-87 -0.3870671241780954 1106 88-89 -0.5023205797244614 1106 90-91 -0.24381944801238475 1106 92-93 -0.17246707309315212 1106 94-95 -0.39896953124695145 1106 96-97 -0.18219407159847734 1106 98-99 -0.11664123179495434 1106 100-101 -0.03929871123508022 1107 1 0.10830191990485005 1107 2 0.017859349436260885 1107 3 0.10494594695568793 1107 4 -0.04404590506203476 1107 5 0.09073213957398707 1107 6 -0.11073710296073358 1107 7 -0.0065833917760897975 1107 8 0.38256215136808436 1107 9 0.1585614187104838 1107 10-11 -0.017776206286313823 1107 12-13 0.012625401433574268 1107 14-15 0.49490992098237996 1107 16-17 0.4767038798606933 1107 18-19 0.5208013241576879 1107 20-21 0.19818184555269625 1107 22-23 0.44697816034336313 1107 24-25 0.3536658621024955 1107 26-27 0.19276672745996137 1107 28-29 0.11318974038352536 1107 30-31 0.34038301756378786 1107 32-33 0.6284796132422841 1107 34-35 1.0306637102991658 1107 36-37 0.40047358255321086 1107 38-39 0.5610952334356583 1107 40-41 0.30072782901415707 1107 42-43 0.08948421220228298 1107 44-45 -0.23057385878724546 1107 46-47 -0.36945917068876 1107 48-49 -0.2836611969126217 1107 50-51 -0.47781781404279755 1107 52-53 -0.9628691926290465 1107 54-55 -0.6734367235889032 1107 56-57 -0.8193576488436385 1107 58-59 -0.5590357459263373 1107 60-61 -0.16313411394132515 1107 62-63 0.14575420939034345 1107 64-65 -0.1727545793918246 1107 66-67 0.09539509145339053 1107 68-69 1.5284650807145432 1107 70-71 1.5579699325503178 1107 72-73 0.49632366184130206 1107 74-75 0.193858137262513 1107 76-77 0.05798545908129782 1107 78-79 0.4347128116225072 1107 80-81 -0.17939237764211668 1107 82-83 -0.13418338188817813 1107 84-85 0.20085553219712438 1107 86-87 0.27698608888392684 1107 88-89 0.422037856748517 1107 90-91 0.17732243017795568 1107 92-93 0.149861785028655 1107 94-95 0.8774272242246965 1107 96-97 0.25621932606602016 1107 98-99 0.7788818085518692 1107 100-101 0.9428179239652579 1108 1 0.22689040212969758 1108 2 -0.0076883352023315865 1108 3 0.18713143823208966 1108 4 -0.21281148291852503 1108 5 0.37356042240227083 1108 6 0.11906087683724564 1108 7 0.2610605804133357 1108 8 0.2677451501874515 1108 9 0.2594075422838813 1108 10-11 -0.07129844162673038 1108 12-13 -0.563788084070822 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-1.3920066309834631 2316 98-99 -1.354474867451863 2316 100-101 -1.6850532244941263 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 20.0 5 93.0 6 236.0 7 405.0 8 685.0 9 1216.0 10 1984.0 11 2917.0 12 3887.0 13 4716.0 14 5458.0 15 6296.0 16 7088.0 17 7599.0 18 6587.0 19 5662.0 20 5724.0 21 5591.0 22 5423.0 23 5201.0 24 5396.0 25 5300.0 26 4910.0 27 5242.0 28 6185.0 29 7092.0 30 8027.0 31 9457.0 32 10971.0 33 12734.0 34 16406.0 35 21300.0 36 19988.0 37 19269.0 38 7481.0 39 284.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.654496695314315 23.287370399307388 15.644572080157104 15.413560825221193 2 14.123048203100913 28.715330131900714 34.78073565373845 22.38088601125992 3 24.522876589707646 31.736306980357714 32.439325102602645 11.301491327331993 4 10.147950480501274 23.57791889746491 35.199885152595044 31.074245469438765 5 7.807934604536472 44.646506443277204 32.57443969666773 14.971119255518586 6 21.644513503014746 40.84683071830296 14.330591633028764 23.178064145653533 7 16.263152561266022 41.90452464996876 20.38625884578358 21.44606394298164 8 22.930635545271834 30.1993784728673 20.53615159857454 26.333834383286327 9 19.51350301474438 23.30051174652502 23.772990592646387 33.41299464608421 10-11 22.133037207181342 29.17292979107906 24.33012717661166 24.363905825127937 12-13 28.743729838369163 28.545280278336065 25.05890151835025 17.652088364944518 14-15 22.150348764545928 30.428439933118277 30.444695907716735 16.97651539461906 16-17 15.353727278485021 35.14408765596301 29.614921780986762 19.887263284565204 18-19 14.714401526794912 34.841831478322554 32.390346062254046 18.053420932628487 20-21 17.573553007144184 29.574473475316253 30.781004577006875 22.07096894053269 22-23 14.515951966761808 32.53327196878853 33.07647486024084 19.87430120420882 24-25 19.217095374014086 32.93946021719671 31.686483473796212 16.15696093499299 26-27 17.178662737059 29.61836218963031 37.558928281141476 15.644046792169213 28-29 12.626669931936025 34.62902599267003 34.372730497052814 18.37157357834113 30-31 14.039973061357191 30.873246929818084 34.91615452985101 20.170625478973715 32-33 14.124780435076342 32.19476253209026 36.65467504391298 17.025781988920414 34-35 16.089769980259895 32.02351922813018 35.615162935047664 16.271547856562265 36-37 14.926149079742931 30.21642789364791 37.03218416553427 17.82523886107489 38-39 15.195110921393523 30.353663639168417 36.77184722474631 17.67937821469175 40-41 14.048505648989185 31.419411558343302 36.7729541698543 17.759128622813208 42-43 14.732746669371746 31.107349360925134 34.50983127071076 19.650072698992357 44-45 14.471409672824715 29.267627778411757 35.47005295835605 20.79090959040748 46-47 17.999378741550146 29.844283893980343 34.13392557154721 18.022411792922302 48-49 16.232276060266678 28.32533863026435 34.97770640069311 20.46467890877586 50-51 11.485324260930204 29.530249561524 38.49417830653171 20.490247871014095 52-53 14.297394596513172 31.097303327107856 34.731389069643626 19.873913006735343 54-55 12.533139548393777 28.079310993140773 34.81432346734209 24.573225991123355 56-57 16.92544222744091 27.908479616144895 31.718731521059656 23.447346635354542 58-59 17.310554953790803 28.89526548382942 33.051031762621754 20.743147799758027 60-61 15.707897337150674 31.48082461340968 32.66973236379606 20.141545685643585 62-63 13.041339517758407 32.75810016826309 31.733743188722784 22.46681712525572 64-65 12.580625410384657 34.76873834824266 29.96552241043322 22.685113830939464 66-67 14.393414064878396 30.88446707032439 28.62573148868518 26.096387376112034 68-69 13.683320333695063 29.896907216494846 30.251500610417796 26.168271839392293 70-71 15.907477457431366 29.15773970352946 28.987147292242817 25.94763554679636 72-73 14.595963974784887 26.460854615545653 32.52736888627612 26.41581252339334 74-75 12.489784368341361 29.853718489923743 30.19645689161822 27.460040250116673 76-77 13.421971250398595 27.0004244652477 30.13703258668277 29.44057169767093 78-79 13.674280731608961 27.102805711498796 31.044187475324136 28.178726081568108 80-81 14.840360510998371 26.28609912996848 30.013026193839053 28.860514165194097 82-83 13.500059112634904 25.91835700653617 31.699994933202724 28.88158894762621 84-85 13.514203921701093 26.294144471279708 30.514786603388 29.676865003631203 86-87 13.734878713028056 26.56385247112969 29.978888255536557 29.7223805603057 88-89 13.438624195645932 26.15945211032107 28.57694776132007 31.824975932712935 90-91 14.200090359029357 23.749435256066512 29.895749325474068 32.15472505943006 92-93 15.020120002195677 24.74591371833686 30.13566750693538 30.09829877253208 94-95 14.1407867494824 23.627836227658765 31.15096970465205 31.080407318206788 96-97 12.074342628977325 24.294802899548344 32.43537144920406 31.19548302227027 98-99 13.968406805142921 24.978092541328554 29.54793563113541 31.505565022393117 100-101 12.857728023242556 24.19776442419354 30.413163462228738 32.53134409033517 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6204.0 1 3732.0 2 1346.0 3 1511.0 4 1222.0 5 937.0 6 1402.0 7 2343.5 8 2999.0 9 2699.0 10 1860.0 11 1248.5 12 1013.5 13 918.5 14 868.5 15 896.5 16 905.5 17 857.5 18 879.5 19 921.0 20 981.0 21 1140.0 22 1297.5 23 1354.5 24 1467.0 25 1697.5 26 1987.0 27 2249.5 28 2475.0 29 2809.0 30 3188.5 31 3519.5 32 3765.0 33 3879.5 34 4047.5 35 4268.5 36 4281.5 37 4143.0 38 3969.0 39 3814.0 40 3812.0 41 3869.0 42 3843.0 43 3904.0 44 4072.5 45 4563.5 46 6690.0 47 12777.5 48 20787.5 49 23224.0 50 20530.5 51 15233.5 52 8102.0 53 3743.5 54 2389.0 55 1877.0 56 1572.0 57 1296.0 58 1035.5 59 879.5 60 771.5 61 661.0 62 541.0 63 423.5 64 365.0 65 316.5 66 264.5 67 256.0 68 219.0 69 154.5 70 135.5 71 130.0 72 110.0 73 83.5 74 58.0 75 44.5 76 34.0 77 21.5 78 14.5 79 10.5 80 7.0 81 4.5 82 4.5 83 4.0 84 2.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02153388842912395 2 0.028289618132378524 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.222331064534108E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.333496596801163E-4 28-29 0.0 30-31 6.333496596801163E-4 32-33 0.0016889324258136432 34-35 0.0035889814048539917 36-37 0.10323599452785893 38-39 0.09584691516492425 40-41 0.10344711108108565 42-43 0.10302487797463222 44-45 0.09922478001655154 46-47 0.09268016686652367 48-49 0.09331351652620379 50-51 0.09331351652620379 52-53 0.07346856052289348 54-55 0.0622793832018781 56-57 0.033778648516272866 58-59 0.01372257595973585 60-61 0.009289128341975037 62-63 0.0019000489790403487 64-65 0.006544613150027868 66-67 0.14018139134253238 68-69 0.39352125521457887 70-71 0.3772652806161225 72-73 0.16488202807005692 74-75 0.02807850157915182 76-77 0.02850073468560523 78-79 0.006544613150027868 80-81 0.0023222820854937594 82-83 0.0 84-85 0.0 86-87 4.222331064534108E-4 88-89 0.0 90-91 0.0012666993193602325 92-93 0.0029556317451738755 94-95 0.07009069567126619 96-97 0.017100440811363138 98-99 0.018789373237176783 100-101 0.012244760087148912 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 236836.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.010032258609336 #Duplication Level Percentage of deduplicated Percentage of total 1 83.59487510552714 28.430643989933962 2 7.81273278045389 5.314225877822628 3 2.8678551919352433 2.926075427722137 4 1.4451010577543826 1.9659173436470807 5 0.8665640363509957 1.4735935415224035 6 0.6691662114515569 1.3655018662703304 7 0.44321398420817404 1.0551605330270737 8 0.326513383324229 0.8883784559779763 9 0.24829915081690418 0.7600195916161395 >10 1.4599990068033968 8.959786518941378 >50 0.1179420966380295 2.9319866912124843 >100 0.09807816457267715 6.721106588525393 >500 0.01365645329492973 3.2444391899880083 >1k 0.031037393852113022 22.082369234406933 >5k 0.004965983016338084 11.880795149386072 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9299 3.926345656910267 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7425 3.135080815416575 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 6263 2.644445945717712 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5151 2.174922731341519 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4961 2.094698441115371 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3806 1.6070192031616815 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3455 1.4588153827965344 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3360 1.4187032376834603 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3140 1.32581195426371 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 2653 1.1201844314208989 RNA PCR Primer, Index 39 (95% over 23bp) CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2539 1.0720498572852102 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2200 0.9289128341975037 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2076 0.8765559289972809 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1928 0.8140654292421761 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1898 0.8013984360485736 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1866 0.7878869766420646 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1844 0.7785978483000895 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1781 0.7519971625935247 No Hit ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT 1583 0.6683950075157493 RNA PCR Primer, Index 39 (96% over 25bp) GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1553 0.655728014322147 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1480 0.624904997551048 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1448 0.6113935381445388 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1444 0.6097046057187252 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1327 0.5603033322636761 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1297 0.5476363390700738 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1226 0.5176577885118817 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1173 0.49527943386985085 No Hit TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 1142 0.4821902075697952 RNA PCR Primer, Index 39 (96% over 30bp) GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1119 0.4724788461213667 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 992 0.41885524160178356 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 867 0.3660761032951072 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 765 0.3230083264368592 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 722 0.3048523028593626 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 719 0.30358560354000236 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 646 0.2727625867689034 No Hit CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT 626 0.26431792463983517 RNA PCR Primer, Index 39 (96% over 28bp) GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 624 0.2634734584269283 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 615 0.25967336046884765 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 594 0.250806465233326 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 514 0.21702781671705315 No Hit TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC 478 0.20182742488473035 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 441 0.18620479994595415 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 436 0.18409363441368712 No Hit CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG 432 0.18240470198787345 Illumina PCR Primer Index 11 (96% over 25bp) CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 428 0.1807157695620598 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 371 0.1566484824942154 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 367 0.15495955006840176 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 360 0.15200391832322788 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 341 0.14398148930061308 No Hit TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT 338 0.14271478998125284 RNA PCR Primer, Index 39 (95% over 22bp) AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 314 0.13258119542637098 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 314 0.13258119542637098 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 303 0.12793663125538346 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 302 0.12751439814893006 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 295 0.1245587664037562 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 295 0.1245587664037562 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 289 0.12202536776503572 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 253 0.10682497593271292 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 250 0.10555827661335271 No Hit CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA 249 0.10513604350689928 RNA PCR Primer, Index 39 (96% over 30bp) CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 248 0.10471381040044589 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 243 0.10260264486817883 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.222331064534108E-4 0.0 16-17 0.0 0.0 0.0 0.0027445151919471703 0.0 18-19 0.0 0.0 0.0 0.004433447617760813 0.0 20-21 0.0 0.0 0.0 0.009500244895201742 0.0 22-23 0.0 0.0 0.0 0.028289618132378524 0.0 24-25 0.0 0.0 0.0 0.06692394737286561 0.0 26-27 0.0 0.0 0.0 0.13363677819250452 0.0 28-29 0.0 0.0 0.0 0.36248712189025317 0.0 30-31 0.0 0.0 0.0 0.8296880541809522 0.0 32-33 0.0 0.0 0.0 1.5932966272019455 0.0 34-35 0.0 0.0 0.0 2.636634633248324 0.0 36-37 0.0 0.0 0.0 3.861110641963215 0.0 38-39 0.0 0.0 0.0 5.303458933608066 0.0 40-41 0.0 0.0 0.0 6.732929115506089 0.0 42-43 0.0 0.0 0.0 7.949804928304819 0.0 44-45 0.0 0.0 0.0 9.104823591008124 0.0 46-47 0.0 0.0 0.0 10.409523889949163 0.0 48-49 0.0 0.0 0.0 11.443783884206793 0.0 50-51 0.0 0.0 0.0 12.460098971440154 0.0 52-53 0.0 0.0 0.0 13.28239794625817 0.0 54-55 0.0 0.0 0.0 14.180065530578121 0.0 56-57 0.0 0.0 0.0 15.340362107112096 0.0 58-59 0.0 0.0 0.0 16.132471414818692 0.0 60-61 0.0 0.0 0.0 16.979471026364237 0.0 62-63 0.0 0.0 0.0 17.73083483929808 0.0 64-65 0.0 0.0 0.0 18.420552618689726 0.0 66-67 0.0 0.0 0.0 19.21033964431083 0.0 68-69 0.0 0.0 0.0 20.110962860375956 0.0 70-71 0.0 0.0 0.0 21.01939738891047 0.0 72-73 0.0 0.0 0.0 21.78765052610245 0.0 74-75 0.0 0.0 0.0 22.72585248864193 0.0 76-77 0.0 0.0 0.0 23.44407100271918 0.0 78-79 0.0 0.0 0.0 24.559399753415867 0.0 80-81 0.0 0.0 0.0 25.581414987586346 0.0 82-83 0.0 0.0 0.0 26.363390700738066 0.0 84-85 0.0 0.0 0.0 27.208701379857793 0.0 86-87 0.0 0.0 0.0 27.965131990069075 0.0 88-89 0.0 0.0 0.0 28.7756084379064 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAGAT 35 3.4310415E-6 67.78232 1 GTTTACG 30 1.3062119E-4 63.263496 1 GGAGCTT 25 0.005042533 56.925125 5 TTACGAG 25 0.005042533 56.925125 3 GTACACG 35 2.8015417E-4 54.2144 3 TGGGCAG 60 1.500759E-6 47.437603 6 GGAGATG 55 4.983667E-5 43.1342 2 AGCTTCC 45 9.671939E-4 42.16676 7 CGCATAG 50 2.8127033E-8 38.314133 66-67 CATAGTA 50 2.820525E-8 38.301888 68-69 GTACCCA 125 1.3460522E-10 37.9581 1 CTTTGCG 75 6.9514717E-6 37.9581 2 TAGTAAG 60 3.590685E-9 35.816425 70-71 ACCCATG 135 3.110472E-10 35.13897 3 ACGCATA 55 6.50889E-8 34.831028 64-65 CGCAGAA 395 0.0 34.82761 9 AGTACAC 55 0.0025894796 34.507362 2 AGATGTG 70 2.0643073E-4 33.884003 4 ATGGGCA 100 1.3067784E-6 33.20632 5 CGTCCCC 30 0.0037985547 31.91824 68-69 >>END_MODULE