FastQCFastQC Report
Fri 27 May 2016
SRR939775_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939775_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157922
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49600.22985075456851545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34520.1599687106392168No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22830.1057962243306292No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA87600.032.3333051
GGTATCA57200.030.5233141
TACATGG97550.030.2519282
GTACATG99600.029.3427811
ACATGGG104000.027.5108623
ATCAACG106250.027.062253
TATCAAC106500.026.9968432
TCAACGC107750.026.729554
CAACGCA108550.026.3152225
AACGCAG110950.025.7459896
CATGGGA51500.023.7701914
CATGGGG50750.022.9060544
ACGCAGA125350.022.5233527
CGCAGAG128200.021.9475928
GAGTACT55650.020.16303612-13
GCAGAGT133550.019.7898089
ATGGGAT22000.019.6272345
AGAGTAC101050.019.25074610-11
GTACTTT59050.018.92371414-15
AGTACTT59400.018.81024412-13
Produced by FastQC (version 0.11.5)