##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939775_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2157922 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.840523892893255 31.0 30.0 33.0 16.0 34.0 2 29.675883558349188 31.0 30.0 33.0 25.0 34.0 3 29.95870610707894 31.0 30.0 34.0 25.0 34.0 4 33.72922932339538 35.0 33.0 37.0 28.0 37.0 5 33.68717590348493 35.0 33.0 37.0 28.0 37.0 6 33.71612968402009 35.0 33.0 37.0 28.0 37.0 7 33.62371809546406 35.0 33.0 37.0 28.0 37.0 8 33.49712315829766 35.0 33.0 37.0 28.0 37.0 9 34.76919601357232 37.0 34.0 39.0 27.0 39.0 10-11 34.58072905322806 37.0 34.0 39.0 27.0 39.0 12-13 34.454710133174416 37.0 33.0 39.0 27.0 39.0 14-15 35.115475443505375 38.0 33.0 40.0 26.5 41.0 16-17 34.92991822688679 38.0 33.0 40.0 25.5 41.0 18-19 34.75929991908883 38.0 33.0 40.0 25.0 41.0 20-21 34.941375545547984 38.0 34.0 40.0 25.0 41.0 22-23 34.837470955854755 38.0 33.5 40.0 25.0 41.0 24-25 34.61793637582823 38.0 33.0 40.0 24.5 41.0 26-27 34.49230532892292 38.0 33.0 40.0 24.0 41.0 28-29 34.55660445558273 38.0 33.0 40.0 24.0 41.0 30-31 34.345786826400584 38.0 33.0 40.0 24.0 41.0 32-33 34.00832745576531 38.0 33.0 40.0 22.0 41.0 34-35 33.82529674381187 38.0 32.0 40.0 21.5 41.0 36-37 33.6156612704259 38.0 32.0 40.0 20.0 41.0 38-39 33.380371023605115 37.5 31.5 40.0 18.5 41.0 40-41 33.145910046795024 37.0 31.0 40.0 18.0 41.0 42-43 32.74923305847014 37.0 31.0 40.0 15.0 41.0 44-45 32.403622790814495 37.0 30.5 40.0 14.0 41.0 46-47 32.02142663173182 37.0 30.0 39.5 10.5 41.0 48-49 31.656349951481104 36.0 30.0 39.0 9.0 40.5 50-51 31.01482375173894 35.5 28.5 38.5 8.0 40.0 52-53 31.1626581034903 36.0 29.5 39.0 7.5 40.0 54-55 31.248549762224954 36.0 29.5 39.0 6.5 40.0 56-57 30.922848694253084 35.5 28.5 39.0 2.0 40.0 58-59 30.501068157236453 35.0 28.0 39.0 2.0 40.0 60-61 30.069751130949125 35.0 28.0 38.0 2.0 40.0 62-63 29.60086439639616 34.0 27.0 38.0 2.0 40.0 64-65 29.039560280677428 34.0 26.0 38.0 2.0 40.0 66-67 28.42646490466291 34.0 26.0 37.0 2.0 39.0 68-69 27.8572054504287 33.0 25.0 36.5 2.0 39.0 70-71 27.266907932724166 32.5 23.5 36.0 2.0 39.0 72-73 26.59550993965491 32.0 22.0 35.5 2.0 38.0 74-75 26.224899926874095 32.0 21.5 35.0 2.0 37.5 76-77 25.653666351239757 31.5 20.0 35.0 2.0 37.0 78-79 24.978079142804976 31.0 18.0 34.5 2.0 36.5 80-81 24.234261479330577 31.0 11.5 34.0 2.0 36.0 82-83 23.47437465302268 30.0 7.0 34.0 2.0 35.5 84-85 22.683763824642412 29.5 2.0 34.0 2.0 35.0 86-87 21.779133351437167 29.0 2.0 34.0 2.0 35.0 88-89 20.797518863054364 28.0 2.0 33.5 2.0 35.0 90-91 19.40696188277426 25.5 2.0 32.5 2.0 34.5 92-93 17.89739944261192 22.0 2.0 32.0 2.0 34.0 94-95 17.32901073347415 20.0 2.0 32.0 2.0 34.0 96-97 16.929383684859786 19.5 2.0 32.0 2.0 34.0 98-99 15.986490707263748 8.5 2.0 31.5 2.0 34.0 100-101 14.338918413177122 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6357776622492217 1101 2 0.9781313006595056 1101 3 0.8375004083567958 1101 4 0.8289022032366873 1101 5 0.9025756673639975 1101 6 0.9028219607386063 1101 7 0.7487927598404127 1101 8 1.0153029807123488 1101 9 1.010540266176342 1101 10-11 1.1462855740520297 1101 12-13 1.1250804630087785 1101 14-15 1.1256318905464084 1101 16-17 1.107895411397429 1101 18-19 1.0679354444049949 1101 20-21 0.9565291390699997 1101 22-23 0.9997405722104844 1101 24-25 1.3102102650963445 1101 26-27 1.3742622662142097 1101 28-29 1.277751221414725 1101 30-31 1.244106972944401 1101 32-33 1.3525531163430173 1101 34-35 1.3360166488345584 1101 36-37 0.9537933819267153 1101 38-39 0.9895527127396164 1101 40-41 1.1058675218234484 1101 42-43 1.3052057758604292 1101 44-45 1.1521417853374558 1101 46-47 1.0898332554714258 1101 48-49 0.9730108737172039 1101 50-51 1.1455854570604451 1101 52-53 0.9314029152533294 1101 54-55 0.8016282196014259 1101 56-57 0.7547570466395364 1101 58-59 0.7151359382564451 1101 60-61 0.6688144888169809 1101 62-63 0.421545438886465 1101 64-65 0.6950062027156214 1101 66-67 0.7913234775667171 1101 68-69 0.46665960127154804 1101 70-71 0.7386313899156107 1101 72-73 0.7032405610220884 1101 74-75 0.8376324075384041 1101 76-77 0.5747749539001568 1101 78-79 0.35038079475060613 1101 80-81 0.11165060421186723 1101 82-83 -0.2870325776457605 1101 84-85 -0.3241483234930662 1101 86-87 -0.5454321310034231 1101 88-89 -4.748189171221222 1101 90-91 -7.896020610545669 1101 92-93 -10.150315024289693 1101 94-95 -8.198340503081099 1101 96-97 -5.10517841229079 1101 98-99 -3.9039785569564955 1101 100-101 -2.916605955140051 1102 1 1.948508136478484 1102 2 1.2897563342345784 1102 3 1.1973243257471928 1102 4 1.0359872899349938 1102 5 0.8663975553277083 1102 6 0.9671094230599095 1102 7 0.9008117409488392 1102 8 0.9559112118282371 1102 9 1.290314044789767 1102 10-11 1.2198728841663282 1102 12-13 1.2087671553417252 1102 14-15 1.4675676054716504 1102 16-17 1.4880058360848096 1102 18-19 1.447506877239931 1102 20-21 1.337251973104685 1102 22-23 1.1626202689902598 1102 24-25 1.383087872760413 1102 26-27 1.298873839915231 1102 28-29 1.2550628279447054 1102 30-31 1.32443287499472 1102 32-33 1.4798674088789028 1102 34-35 1.5914480512280846 1102 36-37 1.3662337675177838 1102 38-39 1.2769971273395555 1102 40-41 1.3172278345939148 1102 42-43 1.4230465255545184 1102 44-45 1.3263913451309293 1102 46-47 1.5368634282711398 1102 48-49 1.281690254145179 1102 50-51 1.2747154895909567 1102 52-53 1.4667012111318058 1102 54-55 1.278007483635811 1102 56-57 1.4694578554257625 1102 58-59 1.3864735691992323 1102 60-61 1.3082951943411985 1102 62-63 1.6004519360363112 1102 64-65 1.4774773281521014 1102 66-67 1.3942974829984145 1102 68-69 1.2902194544365564 1102 70-71 0.7952628400603565 1102 72-73 0.9144582170342659 1102 74-75 0.6478854889840733 1102 76-77 0.647358593139721 1102 78-79 0.6890034706093857 1102 80-81 0.4786742112193565 1102 82-83 0.41550569793844616 1102 84-85 0.627959892700563 1102 86-87 0.6389042763144488 1102 88-89 -0.03174991972153762 1102 90-91 -5.834639705063232 1102 92-93 -10.488785791592047 1102 94-95 -8.180707769622417 1102 96-97 -4.496014655463856 1102 98-99 -3.0367851346865216 1102 100-101 -2.349668300321655 1103 1 2.3021905894232333 1103 2 1.394373820573378 1103 3 1.151182856409001 1103 4 0.9150552923636468 1103 5 1.172557239602149 1103 6 1.038751803144912 1103 7 1.015383689947015 1103 8 1.0530211086102668 1103 9 1.2362126483113158 1103 10-11 1.1134050638870363 1103 12-13 1.1235425044613336 1103 14-15 1.2279865490053439 1103 16-17 1.2938133649251213 1103 18-19 1.2360238778470958 1103 20-21 1.1207644199777889 1103 22-23 1.312553480890685 1103 24-25 1.0260842297731614 1103 26-27 1.175074507796225 1103 28-29 0.9357747283697151 1103 30-31 1.1958855161604802 1103 32-33 1.1685847737847865 1103 34-35 1.1797282576640313 1103 36-37 1.2323752368559795 1103 38-39 0.9891631868416795 1103 40-41 0.9713186056928791 1103 42-43 0.8674742729740714 1103 44-45 0.8251360324411863 1103 46-47 1.0889718071418102 1103 48-49 1.0856702177153075 1103 50-51 0.9234874993055904 1103 52-53 0.9435667241858425 1103 54-55 0.9586905437450994 1103 56-57 0.9790631984737281 1103 58-59 1.0468424216582477 1103 60-61 1.0199573072738026 1103 62-63 0.8888657186714077 1103 64-65 1.0397587489171265 1103 66-67 0.9897513384932566 1103 68-69 0.9155791994274516 1103 70-71 0.7117777155916265 1103 72-73 0.8038890002765235 1103 74-75 0.5860989073204408 1103 76-77 0.43373685664913 1103 78-79 0.2864655228255373 1103 80-81 0.23574919604024558 1103 82-83 -0.36994512173368577 1103 84-85 -0.28078479460969774 1103 86-87 -0.28789293072865973 1103 88-89 -1.1467043823809142 1103 90-91 -6.702985181687396 1103 92-93 -9.658426046601154 1103 94-95 -8.354960957236262 1103 96-97 -5.202480957831799 1103 98-99 -3.713682523878994 1103 100-101 -2.7901616215116984 1104 1 1.8659783006694965 1104 2 1.282607478755775 1104 3 0.9411950798820676 1104 4 0.7564140962618353 1104 5 0.7217050936665359 1104 6 0.8070618663605842 1104 7 0.9065501490033796 1104 8 0.9628918129088007 1104 9 1.0161559940124505 1104 10-11 0.9886812577573139 1104 12-13 0.8809210524326403 1104 14-15 1.067270526596758 1104 16-17 0.9666194680019728 1104 18-19 0.9095924491995362 1104 20-21 0.62774695406695 1104 22-23 0.8234430299877502 1104 24-25 0.6884574154034269 1104 26-27 0.8221456204320319 1104 28-29 0.7858937157199222 1104 30-31 0.993769639634202 1104 32-33 1.0428451204384857 1104 34-35 0.8848090758040712 1104 36-37 0.8873040942443566 1104 38-39 0.90616172468782 1104 40-41 0.7314670026526713 1104 42-43 1.0267488747952669 1104 44-45 0.9529556745965166 1104 46-47 0.8314825984368426 1104 48-49 0.9805055505468268 1104 50-51 0.8340415690226912 1104 52-53 0.6561448278488875 1104 54-55 0.6025693148697258 1104 56-57 0.7243440052895629 1104 58-59 0.6456243209261743 1104 60-61 0.5739929685070742 1104 62-63 0.7093880806327597 1104 64-65 0.6250541939248215 1104 66-67 0.7301012062665855 1104 68-69 0.4969041806092953 1104 70-71 0.47542071024260935 1104 72-73 0.44259086410636783 1104 74-75 0.226652777198737 1104 76-77 0.17856752657538877 1104 78-79 -0.061840510307739294 1104 80-81 -0.2578535477464321 1104 82-83 -0.37476248572673043 1104 84-85 -0.33079053997510144 1104 86-87 -0.12763537182617313 1104 88-89 -0.45773348557100846 1104 90-91 -3.209712921310768 1104 92-93 -7.760898302314878 1104 94-95 -5.506579818021894 1104 96-97 -2.9744222585159132 1104 98-99 -2.0150467567311594 1104 100-101 -1.3820622383474497 1105 1 2.0538098717802207 1105 2 1.3383810428592895 1105 3 1.162162450585317 1105 4 1.180794334742032 1105 5 0.9839992129293691 1105 6 1.0906939866444745 1105 7 0.8977425878796836 1105 8 1.0245255604425836 1105 9 0.8051443996201186 1105 10-11 0.8134380227314679 1105 12-13 0.8022157437903132 1105 14-15 1.0912350491390939 1105 16-17 0.9331547612337303 1105 18-19 0.9389533936864467 1105 20-21 0.9107458865985976 1105 22-23 1.113596699966692 1105 24-25 0.9688027134752559 1105 26-27 0.9710559170973028 1105 28-29 0.8782653661472395 1105 30-31 0.9747397903016335 1105 32-33 1.0542237932352805 1105 34-35 0.9393125940926268 1105 36-37 1.0829923792763978 1105 38-39 0.8664946668370987 1105 40-41 0.8756906680567482 1105 42-43 0.7729506954586967 1105 44-45 0.7858992446388342 1105 46-47 0.8025083389160486 1105 48-49 0.6305869130418422 1105 50-51 0.7008084706839419 1105 52-53 0.7147535291841223 1105 54-55 0.8179142435425675 1105 56-57 0.8073457083136155 1105 58-59 0.9276274153530792 1105 60-61 0.8676153186613256 1105 62-63 0.727424223008601 1105 64-65 0.7441556498304216 1105 66-67 0.7633599020608379 1105 68-69 0.6779855772026799 1105 70-71 0.6268738216713388 1105 72-73 0.6087511463271973 1105 74-75 0.6385096796082621 1105 76-77 0.701358852139979 1105 78-79 0.327331623937539 1105 80-81 0.2725346100797559 1105 82-83 0.2176036000363446 1105 84-85 0.009326119066791705 1105 86-87 0.36526920767938265 1105 88-89 -0.18272505569667175 1105 90-91 -3.3414371668606933 1105 92-93 -8.175273748080004 1105 94-95 -5.653217482132131 1105 96-97 -3.2889439483931504 1105 98-99 -2.3172888901902766 1105 100-101 -1.8712274818808368 1106 1 1.6917603828076544 1106 2 1.1248253677651512 1106 3 0.7992791800097372 1106 4 0.7812426044210738 1106 5 0.9337392165155265 1106 6 0.9364892170550903 1106 7 0.8368406692854577 1106 8 -0.15779468341612102 1106 9 -0.3691249208030456 1106 10-11 -0.5462042580646624 1106 12-13 -0.22308907959143198 1106 14-15 0.7683105377530453 1106 16-17 0.9388298553703649 1106 18-19 0.8787911200626368 1106 20-21 0.7561825012659824 1106 22-23 0.6599926117472208 1106 24-25 0.7029563803212326 1106 26-27 0.7670439034699115 1106 28-29 0.7116659399324305 1106 30-31 0.8478122306817681 1106 32-33 0.8548334399987851 1106 34-35 1.0172622085807035 1106 36-37 0.7283617534919244 1106 38-39 0.7286517405326265 1106 40-41 0.6313523141030046 1106 42-43 0.8757837598302203 1106 44-45 0.8951246346334543 1106 46-47 0.9630830206445822 1106 48-49 0.6884226670314355 1106 50-51 0.5908255049317468 1106 52-53 0.8644307750152151 1106 54-55 0.9888753337344269 1106 56-57 1.031978127176803 1106 58-59 0.8156451668323683 1106 60-61 0.40935370847663677 1106 62-63 0.6557817629045992 1106 64-65 0.5999428113868142 1106 66-67 0.7060441408989213 1106 68-69 0.529594865350429 1106 70-71 0.420750457855668 1106 72-73 0.4475219908672692 1106 74-75 0.5897110423480889 1106 76-77 0.41365488943601747 1106 78-79 0.37199273206018546 1106 80-81 0.16213027082926246 1106 82-83 0.009884396163297282 1106 84-85 -0.2416017991688193 1106 86-87 -0.13772075107980797 1106 88-89 -0.7795737198530297 1106 90-91 -3.9302259421879313 1106 92-93 -8.48331570612196 1106 94-95 -6.182390872843982 1106 96-97 -3.5021948001055403 1106 98-99 -2.4145409970577703 1106 100-101 -1.576015002052971 1107 1 1.6984317108465028 1107 2 0.956034993846572 1107 3 0.79011680076189 1107 4 0.8676157437856702 1107 5 0.7085257152336482 1107 6 0.7011247081863061 1107 7 0.5586012765161499 1107 8 0.5018959267018417 1107 9 0.5746526691283904 1107 10-11 0.6504019846954279 1107 12-13 0.5080793246538988 1107 14-15 0.7301660769590157 1107 16-17 0.615883555073637 1107 18-19 0.6124686644239361 1107 20-21 0.6771461529988656 1107 22-23 0.5040488404188324 1107 24-25 0.4544400185570012 1107 26-27 0.46126067119094927 1107 28-29 0.12364601430567035 1107 30-31 0.18002370391888434 1107 32-33 0.454552888008827 1107 34-35 0.512776681445601 1107 36-37 0.2671626328911003 1107 38-39 0.4223821949468416 1107 40-41 0.3962107857990915 1107 42-43 0.25839631979320643 1107 44-45 0.20973055735903046 1107 46-47 0.1073984076950083 1107 48-49 0.1361523352739269 1107 50-51 0.33633806454686876 1107 52-53 0.1267129689213391 1107 54-55 0.31050601391211785 1107 56-57 0.24774121815356764 1107 58-59 0.17810790194467785 1107 60-61 -0.04381532888043793 1107 62-63 0.08507274732378889 1107 64-65 0.2486825015794949 1107 66-67 0.23994710087025695 1107 68-69 0.3690789488516053 1107 70-71 0.46507471542778944 1107 72-73 0.2838820853470203 1107 74-75 0.361612708266847 1107 76-77 0.2578347836159125 1107 78-79 0.08324803263172242 1107 80-81 -0.09744571823390658 1107 82-83 -0.3136830145835994 1107 84-85 -0.15509125368391352 1107 86-87 -0.03603987418525634 1107 88-89 -0.13779930243758542 1107 90-91 -2.933211133634659 1107 92-93 -4.288597666959477 1107 94-95 -2.702906476265568 1107 96-97 -1.3415466954403428 1107 98-99 -0.9883724362738242 1107 100-101 -0.7064231504098384 1108 1 1.5419497958362243 1108 2 0.8759826186827162 1108 3 -0.5678165442461918 1108 4 -0.24171509046784934 1108 5 0.40028698276927344 1108 6 0.5781949913868587 1108 7 0.6280748162385024 1108 8 0.4728614649479965 1108 9 0.791210196446201 1108 10-11 0.5643753384278227 1108 12-13 0.7553607327163618 1108 14-15 0.5424113482588879 1108 16-17 0.8032481078538751 1108 18-19 0.5760040075235224 1108 20-21 0.6639109461616641 1108 22-23 0.48094383143929065 1108 24-25 0.4672318048104529 1108 26-27 0.6484342270576633 1108 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0.5991855316182786 2311 84-85 0.8989547336954082 2311 86-87 1.3426726600828331 2311 88-89 1.782500484528871 2311 90-91 2.3161118156882914 2311 92-93 3.151819429013173 2311 94-95 2.7378554089407636 2311 96-97 2.1310932216440186 2311 98-99 1.8627259148245265 2311 100-101 1.7339322232788685 2312 1 1.0517769697263368 2312 2 0.4693143143716618 2312 3 0.015868333456118933 2312 4 -0.6853940998475707 2312 5 -0.14832307382011578 2312 6 -0.24243099440329274 2312 7 -0.034316715245502394 2312 8 0.06269413407066793 2312 9 0.08158337538767313 2312 10-11 0.07608478637487792 2312 12-13 0.1326388701114709 2312 14-15 0.16290774628388505 2312 16-17 -0.11567890614798415 2312 18-19 -0.5741729225766719 2312 20-21 0.23361758756513495 2312 22-23 0.3262106701341665 2312 24-25 0.4191717073293475 2312 26-27 0.4356765007380332 2312 28-29 0.2926649789837015 2312 30-31 0.33051391196958235 2312 32-33 0.4934130485177022 2312 34-35 0.31743738928624055 2312 36-37 0.2002537547731933 2312 38-39 0.30743339659244384 2312 40-41 0.17563615899049267 2312 42-43 0.4265222253415928 2312 44-45 5.020987716832792E-4 2312 46-47 0.28296125081042334 2312 48-49 0.1815214659696771 2312 50-51 -0.03617305736976206 2312 52-53 0.096888494512541 2312 54-55 0.09647440208594205 2312 56-57 0.174789758877683 2312 58-59 0.1740564479235296 2312 60-61 0.11583564288878279 2312 62-63 0.19590335892374 2312 64-65 0.2066954006097852 2312 66-67 0.3011523655771775 2312 68-69 0.33871481509305923 2312 70-71 0.35525335932490165 2312 72-73 0.12937545487638857 2312 74-75 0.24174554711186147 2312 76-77 0.542211330896361 2312 78-79 0.44889234395608213 2312 80-81 0.5115042884640353 2312 82-83 0.6118758196337843 2312 84-85 0.9597114974316092 2312 86-87 1.4547793792814545 2312 88-89 1.7527266970547046 2312 90-91 2.435110998220832 2312 92-93 3.2456264805206025 2312 94-95 2.9309059758897753 2312 96-97 2.3266062239132133 2312 98-99 2.1595259266895823 2312 100-101 2.0501165957788086 2313 1 -7.450648951644119 2313 2 -2.770839664062567 2313 3 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62-63 -0.6636349094298417 2313 64-65 -0.8900492789147378 2313 66-67 -0.6889081364140992 2313 68-69 -0.7873199240223592 2313 70-71 -0.569509403447519 2313 72-73 -0.5678278621483663 2313 74-75 -0.634596418210478 2313 76-77 -0.3689973029862905 2313 78-79 -0.4706857588499602 2313 80-81 -0.21758802803138977 2313 82-83 0.019741542978888305 2313 84-85 -0.04255096298270544 2313 86-87 0.4712073840198556 2313 88-89 1.187445482577317 2313 90-91 1.650106995660483 2313 92-93 2.9464973075990954 2313 94-95 2.488623860858638 2313 96-97 1.605837196387995 2313 98-99 1.4047431752325927 2313 100-101 1.166227825689413 2314 1 0.40862390703650675 2314 2 -0.17115965259811006 2314 3 -1.4251615607404133 2314 4 -0.4914716417317564 2314 5 -0.17865150403650887 2314 6 -3.4663705317768496 2314 7 -2.917570015646742 2314 8 -2.5122795551517108 2314 9 -3.572736700244665 2314 10-11 -2.4817016272278494 2314 12-13 -1.344660798159218 2314 14-15 -1.2626704556895518 2314 16-17 -2.201629518462525 2314 18-19 -3.4125459192233407 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-1.8714200669069605 2314 86-87 -0.3606447273908415 2314 88-89 0.9796145189979946 2314 90-91 2.1048175409333005 2314 92-93 3.11362916952622 2314 94-95 2.646048587421614 2314 96-97 2.027946510511047 2314 98-99 2.065151000675975 2314 100-101 1.563834236134939 2315 1 -3.0003296668420774 2315 2 -2.126565504845882 2315 3 -2.9793551123115556 2315 4 -4.262454600231038 2315 5 -4.2416739644679495 2315 6 -3.3978969626361284 2315 7 -6.022483278208249 2315 8 -3.3230670768052235 2315 9 -2.5665186499739647 2315 10-11 -3.962872965131247 2315 12-13 -3.1429442111972286 2315 14-15 -2.2095205171337113 2315 16-17 -2.656106552165511 2315 18-19 -2.147103595301573 2315 20-21 -1.5532971967548264 2315 22-23 -0.8832162571910871 2315 24-25 -2.5275251540595107 2315 26-27 -4.020002277581575 2315 28-29 -3.3961759970872265 2315 30-31 -3.405835324239 2315 32-33 -4.269072565578313 2315 34-35 -4.0466873255279765 2315 36-37 -3.0881212771820827 2315 38-39 -2.111840675981007 2315 40-41 -1.613359649441847 2315 42-43 -3.0287670121210866 2315 44-45 -6.793583112084907 2315 46-47 -3.536163391652231 2315 48-49 -2.4862790945138222 2315 50-51 -3.4928451745214275 2315 52-53 -3.3665044082807114 2315 54-55 -1.9488890680883273 2315 56-57 -1.3043097052383494 2315 58-59 -2.8596183848237544 2315 60-61 -3.327413617919337 2315 62-63 -5.247619583759342 2315 64-65 -4.712177844261692 2315 66-67 -4.072203155571188 2315 68-69 -2.2867154114638275 2315 70-71 -3.8855673004249525 2315 72-73 -2.9104076833488755 2315 74-75 -1.9889599771717386 2315 76-77 -3.580712926655938 2315 78-79 -2.052669581753321 2315 80-81 -1.655604205128025 2315 82-83 -1.304839129647764 2315 84-85 -2.647582891462907 2315 86-87 -2.3055912536983207 2315 88-89 -1.5777652453920332 2315 90-91 -0.7434647369917116 2315 92-93 0.05358968733515468 2315 94-95 0.1309397399561263 2315 96-97 -0.07666599646002581 2315 98-99 -0.4869894769598311 2315 100-101 -0.8508432683931737 2316 1 -7.435785263066311 2316 2 -6.16074645906151 2316 3 -3.523559183460339 2316 4 -4.484641718962173 2316 5 -2.268367600889963 2316 6 -2.871814640668621 2316 7 -3.3559232062888995 2316 8 -2.43990712047054 2316 9 -7.3211529529342165 2316 10-11 -6.965360925061894 2316 12-13 -6.297043935078303 2316 14-15 -5.212845181617116 2316 16-17 -5.341130311235698 2316 18-19 -4.422827283736687 2316 20-21 -3.310915831571503 2316 22-23 -3.9125587088702787 2316 24-25 -3.65558686342829 2316 26-27 -4.721841036972833 2316 28-29 -4.683654324962394 2316 30-31 -7.062463462683741 2316 32-33 -4.969305982389461 2316 34-35 -6.04835848715387 2316 36-37 -4.587528905770025 2316 38-39 -7.03041545283839 2316 40-41 -4.277268337555888 2316 42-43 -4.91694623256673 2316 44-45 -3.352176831984732 2316 46-47 -4.624120432684787 2316 48-49 -5.632980757648735 2316 50-51 -5.186916002236064 2316 52-53 -6.875515838319885 2316 54-55 -5.634962721205902 2316 56-57 -4.856736020013923 2316 58-59 -3.495109298584751 2316 60-61 -3.6185142407922584 2316 62-63 -4.570979886848022 2316 64-65 -3.3482396178441753 2316 66-67 -4.644012055590451 2316 68-69 -3.470075393483988 2316 70-71 -2.8403309939971635 2316 72-73 -2.3004455694337658 2316 74-75 -2.5599771222304923 2316 76-77 -3.1589242539531845 2316 78-79 -4.998896384553042 2316 80-81 -5.992971764365166 2316 82-83 -3.4163378774805473 2316 84-85 -2.504077144336474 2316 86-87 -2.8115695494666397 2316 88-89 -1.8683669277910582 2316 90-91 -1.5891110479144608 2316 92-93 -1.4728981441680862 2316 94-95 -1.9468375027074583 2316 96-97 -3.4711853568915423 2316 98-99 -4.763574913291944 2316 100-101 -4.379638298252546 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17477.0 3 6279.0 4 6199.0 5 6906.0 6 7721.0 7 9058.0 8 10585.0 9 12763.0 10 14951.0 11 17472.0 12 19879.0 13 22201.0 14 24398.0 15 26255.0 16 28659.0 17 31120.0 18 29704.0 19 30409.0 20 32690.0 21 36254.0 22 40081.0 23 44498.0 24 50255.0 25 56229.0 26 63280.0 27 71965.0 28 81618.0 29 92397.0 30 105192.0 31 118741.0 32 131902.0 33 146687.0 34 167171.0 35 192483.0 36 203434.0 37 156320.0 38 44003.0 39 686.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.8692784071557 19.087784066916686 12.924180455286635 23.11875707064098 2 16.074855244599508 19.879880855170367 39.47558644495583 24.569677455274295 3 19.997383100209632 24.173101425282407 28.732074932419955 27.09744054208801 4 13.120645210688856 15.71643027348599 36.399460777603245 34.76346373822191 5 14.20998368593854 37.30843969643596 33.59880029472958 14.882776322895921 6 33.12380764102842 35.0852744044064 17.350537179324757 14.440380775240422 7 29.73380224938585 32.06544646985314 20.216619905040787 17.984131375720224 8 27.205675536432878 34.10761611688969 19.536182240973584 19.15052610570385 9 27.229566197434153 16.378142671069646 19.57045868306056 36.821832448435636 10-11 25.653706753842265 25.961074077520895 26.978004112929412 21.407215055707425 12-13 26.52017831078149 23.25243135243762 27.331557341860567 22.895832994920323 14-15 23.43974453884481 24.671286379659648 25.44347156114893 26.445497520346617 16-17 22.9621359863143 27.937403522972094 26.03932967552159 23.061130815192016 18-19 22.94194879250397 26.640157061634717 28.05541069115521 22.362483454706105 20-21 23.786455990533874 25.78972215761995 28.01935932804972 22.404462523796457 22-23 23.361199761775122 25.6464403839925 28.008140510883234 22.98421934334915 24-25 23.062576029436833 25.940930323941718 28.24247114074695 22.7540225058745 26-27 22.843697071445362 26.126881736414287 28.114277830374057 22.915143361766294 28-29 22.74701267765533 26.40654061476316 28.117085755800453 22.729360951781057 30-31 22.85954487975206 26.110367298784574 27.95072801357209 23.079359807891276 32-33 22.71911996913965 26.133893687400555 27.946567564259684 23.200418779200106 34-35 23.2677304684561 26.206832011399072 27.82071729060292 22.704720229541913 36-37 23.388716416690354 25.847095500054806 27.887105644021098 22.87708243923374 38-39 22.918422725738054 26.184518443902938 27.89817667080458 22.998882159554423 40-41 23.124536293623233 26.09030649032182 27.99011224161613 22.79504497443882 42-43 22.93141529219229 26.02999663773134 27.737880507572537 23.30070756250383 44-45 22.856894089785115 26.127955978128377 27.90473536957708 23.110414562509433 46-47 23.220830538005657 26.38083381692792 27.394814983560106 23.003520661506315 48-49 22.925694702886137 26.4677513551637 27.40041805992874 23.206135882021425 50-51 22.81995241327689 26.639969409198894 27.425089939464225 23.114988238059993 52-53 23.120096253004714 26.431801777167973 27.242745886910154 23.20535608291716 54-55 22.634555845661954 26.603471535126427 27.401626789878897 23.360345829332722 56-57 22.65568897043454 26.741226745941425 27.23694172840653 23.366142555217504 58-59 22.771617729234396 26.271813516123505 27.393744388255385 23.56282436638671 60-61 22.5135503368545 26.522532194820226 27.595994709889055 23.367922758436222 62-63 22.602927048523032 26.347453168755923 27.64676684139435 23.402852941326696 64-65 22.426694136993632 26.468769799728037 27.4687482079396 23.635787855338734 66-67 22.41283313891716 26.5487662622315 27.26403511378918 23.77436548506215 68-69 22.654633220993826 26.310059358445837 27.375864458883857 23.659442961676476 70-71 22.75497627382486 26.233143455820272 27.081555069896783 23.930325200458082 72-73 22.594423416040925 26.4548852480949 27.069060188307958 23.881631147556213 74-75 22.580793519980155 26.842946112257536 26.903570867784143 23.672689499978166 76-77 22.739186230634274 26.425527446549108 27.029196340955725 23.806089981860897 78-79 22.8105648932137 26.67285684019595 26.842765255982336 23.673813010608008 80-81 22.892038221666052 26.64901043226486 26.725705620123925 23.733245725945164 82-83 22.9745261865115 26.65174589521957 26.731437963210148 23.642289955058786 84-85 22.818616725246933 26.774062274400933 26.60937785991094 23.7979431404412 86-87 22.75415330160794 26.514906032864978 26.948720217957018 23.78222044757006 88-89 22.859555370754727 26.55138811619688 26.566254775043063 24.022801738005338 90-91 22.591426697335816 26.542896074507222 26.927238049483098 23.93843917867386 92-93 22.941777647644642 26.221109355971528 26.990197742272983 23.84691525411085 94-95 22.830474673147336 26.16781424626307 26.92720943658969 24.074501643999906 96-97 23.20241389967491 25.97170615860446 26.58521962815535 24.240660313565286 98-99 23.203154545828568 26.216165141329522 26.177858348079972 24.402821964761934 100-101 23.380943468824796 26.112053342570945 26.24027173464489 24.266731453959366 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2690.0 1 1564.0 2 423.0 3 417.5 4 388.0 5 345.0 6 440.5 7 722.0 8 1067.5 9 1257.0 10 1188.0 11 1149.0 12 1173.5 13 1199.0 14 1331.0 15 1483.0 16 1606.0 17 1798.0 18 2048.0 19 2303.0 20 2630.0 21 3069.0 22 3634.5 23 4421.5 24 5415.0 25 6722.5 26 8573.0 27 10861.5 28 13014.0 29 15654.5 30 19018.5 31 23242.0 32 28871.5 33 33057.5 34 36161.0 35 40116.5 36 45297.0 37 50458.5 38 54167.5 39 57100.5 40 59618.0 41 62462.5 42 65141.5 43 67649.5 44 70825.5 45 74846.5 46 77771.0 47 80745.0 48 84676.0 49 85871.5 50 84575.5 51 83242.0 52 82308.0 53 82047.0 54 81376.5 55 77604.5 56 71726.0 57 65802.5 58 60128.5 59 53209.5 60 44760.5 61 36952.5 62 29937.0 63 22937.5 64 16835.0 65 12607.0 66 9362.5 67 6993.0 68 5373.5 69 4124.5 70 3162.5 71 2276.5 72 1674.5 73 1267.0 74 970.0 75 705.0 76 480.0 77 335.0 78 233.5 79 180.5 80 111.5 81 72.5 82 57.5 83 35.0 84 25.0 85 23.0 86 17.0 87 14.0 88 10.0 89 5.0 90 2.5 91 3.0 92 3.0 93 2.5 94 2.5 95 2.0 96 0.5 97 0.5 98 2.5 99 3.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1336470919708868 2 0.11872532927510819 3 0.12512037043044189 4 0.13749338483967447 5 0.1263252332568091 6 0.14157138209814812 7 0.1730368382175074 8 0.15811507552172877 9 0.16793934164441532 10-11 0.16203087970742225 12-13 0.15093223944146267 14-15 0.15695655357329874 16-17 0.15125662558702307 18-19 0.1672210580363887 20-21 0.17215636153670058 22-23 0.2082790758887485 24-25 0.23045318598169906 26-27 0.24356765443792686 28-29 0.2390262484000812 30-31 0.23052269729860486 32-33 0.23158853749116048 34-35 0.2207679424928241 36-37 0.22790443769515303 38-39 0.21608751382116684 40-41 0.22032770415242073 42-43 0.21381681080224402 44-45 0.2060778841867315 46-47 0.22130086258910195 48-49 0.25946257557038666 50-51 0.2614088924437491 52-53 0.26310033448845693 54-55 0.2616637672724037 56-57 0.2350409328974819 58-59 0.23499459201954473 60-61 0.2071437243792871 62-63 0.19857066196090498 64-65 0.20044746751736162 66-67 0.2069583608675383 68-69 0.20758396271969048 70-71 0.213353402022872 72-73 0.24458715375254525 74-75 0.24727492467290288 76-77 0.2625674143921791 78-79 0.2588601441572031 80-81 0.2732489867567039 82-83 0.2728782597332063 84-85 0.2465334706259077 86-87 0.24966147988666876 88-89 0.2527663187084612 90-91 0.4557625345123688 92-93 0.6075289097567012 94-95 0.348784617794341 96-97 0.28810123813557675 98-99 0.3182459792337258 100-101 0.3380335341129105 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2157922.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.51396851965129 #Duplication Level Percentage of deduplicated Percentage of total 1 77.89317383523759 44.02052374020343 2 11.711289569447363 13.237029001045375 3 4.332690487603849 7.345726014655096 4 1.9564150506684062 4.422591143393852 5 1.0805589173762884 3.053333632011602 6 0.6673042121977717 2.262720554470534 7 0.4325016946599751 1.7109671009696767 8 0.32570792215375804 1.4725637807358811 9 0.23772207991570085 1.2091156326703751 >10 1.2395330334711747 12.684423059601112 >50 0.08494668818513042 3.302733664127676 >100 0.03546877672705288 3.6368161140179702 >500 0.0018478159947357237 0.7161347315683001 >1k 8.399163612434953E-4 0.9253218305290809 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4960 0.22985075456851545 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3452 0.1599687106392168 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2283 0.1057962243306292 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.6340877937200695E-5 0.0 0.0 0.0 4.6340877937200695E-5 7 9.268175587440139E-5 0.0 0.0 0.0 4.6340877937200695E-5 8 9.268175587440139E-5 0.0 0.0 0.0 4.6340877937200695E-5 9 9.268175587440139E-5 0.0 0.0 0.0 4.6340877937200695E-5 10-11 9.268175587440139E-5 0.0 0.0 0.0 6.951131690580105E-5 12-13 1.390226338116021E-4 0.0 0.0 4.6340877937200695E-5 1.390226338116021E-4 14-15 1.390226338116021E-4 0.0 0.0 4.6340877937200695E-5 1.6219307278020242E-4 16-17 1.390226338116021E-4 0.0 0.0 4.6340877937200695E-5 1.8536351174880278E-4 18-19 1.8536351174880278E-4 0.0 0.0 9.268175587440139E-5 1.8536351174880278E-4 20-21 2.3170438968600347E-4 0.0 0.0 3.475565845290052E-4 1.8536351174880278E-4 22-23 2.3170438968600347E-4 0.0 0.0 7.182836080266107E-4 2.3170438968600347E-4 24-25 3.7072702349760556E-4 0.0 0.0 0.0013670558991474206 3.243861455604049E-4 26-27 3.7072702349760556E-4 0.0 0.0 0.0026414300424204397 3.475565845290052E-4 28-29 7.182836080266108E-4 0.0 0.0 0.009569391294031943 3.7072702349760556E-4 30-31 8.341358028696125E-4 0.0 0.0 0.02944962792909104 3.938974624662059E-4 32-33 8.804766808068132E-4 0.0 0.0 0.07609172157288355 5.097496573092077E-4 34-35 9.963288756498151E-4 0.0 0.0 0.15028346715034185 5.560905352464084E-4 36-37 0.0011353515094614172 0.0 0.0 0.26826734237845484 5.560905352464084E-4 38-39 0.001204862826367218 0.0 0.0 0.4487882323828202 5.560905352464084E-4 40-41 0.001204862826367218 0.0 0.0 0.6541246625225564 5.792609742150086E-4 42-43 0.0013207150212102199 0.0 0.0 0.8576074575448047 6.256018521522094E-4 44-45 0.001529248971927623 0.0 0.0 1.0960312745317022 6.487722911208098E-4 46-47 0.0016914420447078254 0.0 0.0 1.3788728230214067 6.951131690580105E-4 48-49 0.0018072942395508272 0.0 0.0 1.6749678626011506 6.951131690580105E-4 50-51 0.0018072942395508272 0.0 0.0 1.998751576748372 6.951131690580105E-4 52-53 0.0018536351174880277 0.0 0.0 2.306709881080039 7.182836080266107E-4 54-55 0.0018999759954252285 0.0 0.0 2.6273424155275307 7.414540469952111E-4 56-57 0.0020853395071740314 4.6340877937200695E-5 0.0 2.999065767900786 7.414540469952111E-4 58-59 0.0020853395071740314 4.6340877937200695E-5 0.0 3.402069212881652 7.414540469952111E-4 60-61 0.0020853395071740314 4.6340877937200695E-5 0.0 3.8280345628804007 7.877949249324119E-4 62-63 0.0020853395071740314 4.6340877937200695E-5 0.0 4.241209830568482 7.877949249324119E-4 64-65 0.0020853395071740314 4.6340877937200695E-5 0.0 4.655057040986653 7.877949249324119E-4 66-67 0.0022243621409856335 4.6340877937200695E-5 0.0 5.1231462490303175 7.877949249324119E-4 68-69 0.0022938734578914345 4.6340877937200695E-5 0.0 5.624137480409393 7.877949249324119E-4 70-71 0.0023633847747972355 4.6340877937200695E-5 0.0 6.161089233067738 8.341358028696125E-4 72-73 0.0024097256527344365 4.6340877937200695E-5 0.0 6.692688614324336 0.0010194993146184154 74-75 0.002456066530671637 4.6340877937200695E-5 0.0 7.206678461964797 0.0010194993146184154 76-77 0.002456066530671637 4.6340877937200695E-5 0.0 7.741498534238031 0.0010194993146184154 78-79 0.002456066530671637 4.6340877937200695E-5 0.0 8.336538577390657 0.0010194993146184154 80-81 0.0025719187255146387 4.6340877937200695E-5 0.0 8.94436870285395 0.0010194993146184154 82-83 0.0026414300424204397 4.6340877937200695E-5 0.0 9.517512681181248 0.001065840192555616 84-85 0.0026877709203576407 4.6340877937200695E-5 0.0 10.058542431098065 0.001065840192555616 86-87 0.0027341117982948413 6.951131690580105E-5 0.0 10.608284266067077 0.001065840192555616 88-89 0.0027341117982948413 9.268175587440139E-5 0.0 11.189746431984103 0.001065840192555616 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8760 0.0 32.333305 1 GGTATCA 5720 0.0 30.523314 1 TACATGG 9755 0.0 30.251928 2 GTACATG 9960 0.0 29.342781 1 ACATGGG 10400 0.0 27.510862 3 ATCAACG 10625 0.0 27.06225 3 TATCAAC 10650 0.0 26.996843 2 TCAACGC 10775 0.0 26.72955 4 CAACGCA 10855 0.0 26.315222 5 AACGCAG 11095 0.0 25.745989 6 CATGGGA 5150 0.0 23.770191 4 CATGGGG 5075 0.0 22.906054 4 ACGCAGA 12535 0.0 22.523352 7 CGCAGAG 12820 0.0 21.947592 8 GAGTACT 5565 0.0 20.163036 12-13 GCAGAGT 13355 0.0 19.789808 9 ATGGGAT 2200 0.0 19.627234 5 AGAGTAC 10105 0.0 19.250746 10-11 GTACTTT 5905 0.0 18.923714 14-15 AGTACTT 5940 0.0 18.810244 12-13 >>END_MODULE