FastQCFastQC Report
Fri 27 May 2016
SRR939775_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939775_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157922
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57800.26785027447702003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41570.19263902958494328No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30200.13994945137034612No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85850.034.001461
GGTATCA53500.033.847351
ATCAACG102400.028.3140223
TATCAAC104500.027.8816642
TCAACGC104100.027.8050654
CAACGCA104350.027.7836235
AACGCAG106900.027.1064226
GTACATG99400.026.4725041
TACATGG97400.026.3805642
ACATGGG102200.024.079423
ACGCAGA121400.023.6747767
CGCAGAG124000.023.0649138
GAGTACT59150.020.99015812-13
GCAGAGT134050.020.8084499
CATGGGA46850.020.426444
TATAGGG6700.020.4065572
CATGGGG52250.019.6687364
AGAGTAC107000.019.55167810-11
GTACTTT62650.019.5166914-15
AGTACTT61950.019.05268912-13
Produced by FastQC (version 0.11.5)