##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939775_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2157922 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42964018161917 31.0 31.0 34.0 30.0 34.0 2 31.63714443802881 31.0 31.0 34.0 30.0 34.0 3 31.704333150132396 31.0 31.0 34.0 30.0 34.0 4 35.50818472586127 37.0 35.0 37.0 33.0 37.0 5 35.24015696582175 37.0 35.0 37.0 33.0 37.0 6 35.25815112872476 37.0 35.0 37.0 32.0 37.0 7 35.17878171685538 37.0 35.0 37.0 32.0 37.0 8 35.110970646761096 37.0 35.0 37.0 32.0 37.0 9 36.724956231040785 39.0 35.0 39.0 32.0 39.0 10-11 36.58345389685077 38.5 35.0 39.0 32.0 39.0 12-13 36.52297766091638 38.0 35.0 39.0 32.0 39.0 14-15 37.5780278434531 39.0 36.0 41.0 32.0 41.0 16-17 37.503227873852715 39.0 36.0 41.0 32.0 41.0 18-19 37.47321983834448 39.0 36.0 41.0 32.0 41.0 20-21 37.385490995504014 39.0 36.0 41.0 32.0 41.0 22-23 37.23949591319797 39.0 36.0 40.0 31.0 41.0 24-25 37.071529693844354 39.0 36.0 40.0 31.0 41.0 26-27 36.77372954166091 39.0 35.5 40.0 30.0 41.0 28-29 36.54945938731798 38.0 35.0 40.0 30.0 41.0 30-31 36.307179314173545 38.0 35.0 40.0 30.0 41.0 32-33 36.27000975938889 38.0 35.0 40.0 30.0 41.0 34-35 36.3629429145261 38.0 35.0 40.0 30.0 41.0 36-37 36.33475051461545 38.0 35.0 40.0 30.0 41.0 38-39 36.201047118477874 38.0 35.0 40.0 30.0 41.0 40-41 36.0272322169198 38.0 35.0 40.0 29.5 41.0 42-43 35.82361989914371 38.0 35.0 40.0 29.0 41.0 44-45 35.58581380605972 38.0 34.0 40.0 28.0 41.0 46-47 35.29415868599514 38.0 34.0 40.0 27.0 41.0 48-49 34.988692594078934 38.0 34.0 40.0 26.0 41.0 50-51 34.714891919170384 38.0 33.0 40.0 26.0 41.0 52-53 34.42261258747999 38.0 33.0 40.0 24.5 41.0 54-55 34.10327041477866 37.0 33.0 40.0 24.0 41.0 56-57 32.81392283873096 36.5 31.5 39.5 16.0 41.0 58-59 29.450700720415288 34.5 24.5 38.5 7.5 40.0 60-61 29.463907175514223 34.0 25.0 38.0 9.0 40.0 62-63 31.24293950383749 35.0 29.5 38.0 12.5 40.0 64-65 30.71291895629221 35.0 29.0 38.0 4.5 40.0 66-67 25.178645938083026 28.5 18.0 36.0 2.0 39.0 68-69 17.35911446289532 13.0 7.0 29.0 2.0 35.5 70-71 16.24481260212371 13.0 7.5 25.5 2.0 33.0 72-73 18.357015684533547 18.0 7.0 30.0 2.0 34.0 74-75 19.125898202066615 17.5 8.5 30.5 2.0 35.0 76-77 20.188908588910998 23.0 8.5 31.0 2.0 34.5 78-79 23.40890287044666 28.5 12.0 34.0 2.0 35.5 80-81 26.082936964357376 31.5 22.5 34.5 2.0 36.0 82-83 27.029588418858513 33.0 26.0 35.0 2.0 36.0 84-85 27.01839617001912 33.0 26.0 35.0 2.0 36.0 86-87 26.745832564847106 33.0 25.5 35.0 2.0 35.0 88-89 26.31744729420248 33.0 24.5 35.0 2.0 35.0 90-91 24.74354402059018 31.5 12.5 34.5 2.0 35.0 92-93 22.669563589416114 31.0 2.0 34.0 2.0 35.0 94-95 18.100484169492688 23.0 2.0 33.0 2.0 35.0 96-97 16.743298877345893 15.0 2.0 32.5 2.0 34.5 98-99 15.607711956224552 2.0 2.0 32.0 2.0 34.0 100-101 14.980352857980964 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3607411337397046 1101 2 0.32602667140867325 1101 3 0.31514340416618225 1101 4 0.3023681482225484 1101 5 0.44642085078707794 1101 6 0.26227346394769313 1101 7 0.2833480379789748 1101 8 0.354615514949451 1101 9 0.43782890758966175 1101 10-11 0.24347755989153796 1101 12-13 0.4360948745488926 1101 14-15 0.3796911514329935 1101 16-17 0.38271253203416933 1101 18-19 0.5700759357033078 1101 20-21 0.6506130439295674 1101 22-23 0.5546919499984213 1101 24-25 0.5441641273985169 1101 26-27 0.7508021007783086 1101 28-29 0.6747428065336081 1101 30-31 0.6425357674803749 1101 32-33 0.4995676594819116 1101 34-35 0.5939053022255507 1101 36-37 0.6139496671259934 1101 38-39 0.41797847509763386 1101 40-41 0.4917536180576505 1101 42-43 0.4411615220013445 1101 44-45 0.5248724691872724 1101 46-47 0.24253440172607554 1101 48-49 0.061702480534741255 1101 50-51 -0.0169329557583211 1101 52-53 0.3058834198999918 1101 54-55 0.3216429591270824 1101 56-57 1.033555768961591 1101 58-59 -8.32320749110329 1101 60-61 -9.438642361013887 1101 62-63 -2.625262390614072 1101 64-65 -0.06033503399166307 1101 66-67 -1.4911305680957518 1101 68-69 -3.8281920654075066 1101 70-71 -4.601494715346572 1101 72-73 -7.147081429391413 1101 74-75 -7.862350859151185 1101 76-77 -8.977371591522768 1101 78-79 -12.109613478964489 1101 80-81 -8.299983015615705 1101 82-83 -4.527944557162577 1101 84-85 -3.664709467573825 1101 86-87 -3.4626138401595625 1101 88-89 -3.490284491802651 1101 90-91 -8.974650683748887 1101 92-93 -14.94304640484452 1101 94-95 -12.843931070545823 1101 96-97 -12.63193262057324 1101 98-99 -12.052518454572557 1101 100-101 -11.870109121955469 1102 1 0.5214108445464021 1102 2 0.5093549311752916 1102 3 0.4140475137552251 1102 4 0.3935884902406386 1102 5 0.52038330639337 1102 6 0.48931318699064263 1102 7 0.4005677234178364 1102 8 0.5382165535383976 1102 9 0.6548468441402377 1102 10-11 0.7209918106451099 1102 12-13 0.6631417602742644 1102 14-15 0.8212075509912964 1102 16-17 0.8748541740791111 1102 18-19 0.8338449988841319 1102 20-21 0.75762292246241 1102 22-23 0.8627941376010142 1102 24-25 0.7215572094410732 1102 26-27 0.9208874560772031 1102 28-29 0.7761905637186715 1102 30-31 0.7304135243669947 1102 32-33 0.47483939374639306 1102 34-35 0.5627565870317071 1102 36-37 0.6954284626015692 1102 38-39 0.7151363824005799 1102 40-41 0.6453349592699311 1102 42-43 0.666335664717792 1102 44-45 0.5997004155865255 1102 46-47 0.8129775927010954 1102 48-49 0.735529143620063 1102 50-51 0.7841566673055098 1102 52-53 0.7378791222905363 1102 54-55 0.5026812496335467 1102 56-57 1.3380855687049262 1102 58-59 -8.93898688048461 1102 60-61 -10.220488243963718 1102 62-63 -3.1486694398873603 1102 64-65 -0.1717317570644532 1102 66-67 -1.2259036005964248 1102 68-69 -3.692124378058594 1102 70-71 -4.2259719887521285 1102 72-73 -6.811851775886174 1102 74-75 -7.509836757620162 1102 76-77 -8.502968929392466 1102 78-79 -11.684693611474113 1102 80-81 -7.594624732271928 1102 82-83 -3.3967251104797995 1102 84-85 -2.4901695206970516 1102 86-87 -2.3618178870751336 1102 88-89 -2.5470433780055295 1102 90-91 -8.087407421147041 1102 92-93 -14.411741005972642 1102 94-95 -12.629324271966421 1102 96-97 -12.376708339079821 1102 98-99 -11.896638489192187 1102 100-101 -11.590537689120538 1103 1 0.2881776933624316 1103 2 0.3331679244964789 1103 3 0.23757210391915962 1103 4 0.1657378527178679 1103 5 0.2100857957679878 1103 6 0.15060948328693513 1103 7 0.25986948419500777 1103 8 0.36833495062401056 1103 9 0.30656511979293555 1103 10-11 0.41011142205980633 1103 12-13 0.39107284709879053 1103 14-15 0.43871514054806227 1103 16-17 0.43939424693886764 1103 18-19 0.5559245374390756 1103 20-21 0.4666362800823265 1103 22-23 0.41178928028467965 1103 24-25 0.5395687455673652 1103 26-27 0.5068543656096836 1103 28-29 0.5179801812050115 1103 30-31 0.43269342722066995 1103 32-33 0.4793976815423804 1103 34-35 0.567651851148284 1103 36-37 0.35266282811462446 1103 38-39 0.37035344372420553 1103 40-41 0.38977940371437825 1103 42-43 0.34949595615677254 1103 44-45 0.2905261593630968 1103 46-47 0.22617431401257448 1103 48-49 0.18130327841058858 1103 50-51 0.2901083977244525 1103 52-53 0.2897507069899916 1103 54-55 0.22611780093894396 1103 56-57 0.8213703288749343 1103 58-59 -6.618627135493718 1103 60-61 -8.693933902737246 1103 62-63 -2.6371864404945207 1103 64-65 -0.39301591006992 1103 66-67 -1.8148516880281171 1103 68-69 -4.379788312484823 1103 70-71 -4.960710907999246 1103 72-73 -7.824679644738634 1103 74-75 -8.095205610079178 1103 76-77 -8.862871783065811 1103 78-79 -12.312252810016918 1103 80-81 -8.687454119035738 1103 82-83 -4.775850793539906 1103 84-85 -3.8663823318688806 1103 86-87 -3.4856384699530913 1103 88-89 -3.731419516439047 1103 90-91 -9.020731647012251 1103 92-93 -14.64022461253002 1103 94-95 -12.625934775912992 1103 96-97 -12.584973366402224 1103 98-99 -12.1984948947781 1103 100-101 -11.842594459005177 1104 1 0.1506527967790099 1104 2 0.12030230276467435 1104 3 0.1964367864086185 1104 4 0.1407716587809773 1104 5 0.0960231396439255 1104 6 0.12585328661891992 1104 7 0.10470365130021264 1104 8 0.21005546274422926 1104 9 0.16593056500046544 1104 10-11 0.26838341598470805 1104 12-13 0.19262601088000508 1104 14-15 0.2874559895965021 1104 16-17 0.394456413457867 1104 18-19 0.2587127882500937 1104 20-21 0.14839782576881078 1104 22-23 0.21647933031098887 1104 24-25 0.253182263995825 1104 26-27 0.3152611674561996 1104 28-29 0.35454760015372955 1104 30-31 0.192309170521078 1104 32-33 0.27288851560290794 1104 34-35 0.14194300628314238 1104 36-37 0.02342957627111275 1104 38-39 0.06797744369755065 1104 40-41 0.23112438542912628 1104 42-43 0.06414730406945779 1104 44-45 0.10231964595058685 1104 46-47 0.015204329799843208 1104 48-49 0.0972217708267138 1104 50-51 0.1735751512099739 1104 52-53 0.10619552483692729 1104 54-55 -0.09405825704094894 1104 56-57 0.6467755020352541 1104 58-59 -6.505922633791759 1104 60-61 -8.579014437772319 1104 62-63 -2.4927135652805283 1104 64-65 -0.3806806938110334 1104 66-67 -1.8268435978874997 1104 68-69 -4.45748805136213 1104 70-71 -4.954702254979349 1104 72-73 -7.84859293801539 1104 74-75 -8.677398724300375 1104 76-77 -9.39602670476231 1104 78-79 -12.595481005938876 1104 80-81 -9.023353493540306 1104 82-83 -5.107768046955485 1104 84-85 -4.271491627037445 1104 86-87 -3.9828657899149924 1104 88-89 -3.920774020472461 1104 90-91 -9.630976882522273 1104 92-93 -15.346259494391983 1104 94-95 -12.655103163413742 1104 96-97 -12.2393928846575 1104 98-99 -11.674152447685422 1104 100-101 -11.254173368684702 1105 1 0.21044736358292226 1105 2 0.1957859076062647 1105 3 0.19246243217014225 1105 4 0.09283479948694406 1105 5 0.15629887230893047 1105 6 0.19414095181840452 1105 7 0.09672415957427205 1105 8 0.1829562582781037 1105 9 0.1439662432596336 1105 10-11 0.22368282333611944 1105 12-13 0.26028498741748507 1105 14-15 0.4129661627499104 1105 16-17 0.31246878169373105 1105 18-19 0.2758211708603042 1105 20-21 0.2779311327706253 1105 22-23 0.33667655148342135 1105 24-25 0.30264242052628276 1105 26-27 0.328806388996135 1105 28-29 0.19578263831074594 1105 30-31 0.28646506541853256 1105 32-33 0.366152525059519 1105 34-35 0.34525464452975996 1105 36-37 0.1867441839172912 1105 38-39 0.233717060981256 1105 40-41 0.18628290021786853 1105 42-43 0.05100155438368148 1105 44-45 0.14185788774400976 1105 46-47 0.07701022673373359 1105 48-49 0.2802571933481133 1105 50-51 0.12194998509882993 1105 52-53 0.1694408938523111 1105 54-55 0.11041888237117092 1105 56-57 0.852527423146789 1105 58-59 -6.923470174967903 1105 60-61 -8.991660220135696 1105 62-63 -2.91080360202152 1105 64-65 -0.4682585635912595 1105 66-67 -1.508817353510178 1105 68-69 -4.09297519390941 1105 70-71 -4.57665056943071 1105 72-73 -5.970662964697363 1105 74-75 -6.3683565711399766 1105 76-77 -8.295349141772679 1105 78-79 -11.86953432631483 1105 80-81 -8.08516992781712 1105 82-83 -4.196410425165112 1105 84-85 -3.3629369384644683 1105 86-87 -3.0406601559174042 1105 88-89 -3.2489703399324945 1105 90-91 -8.634229487969108 1105 92-93 -11.64985289408453 1105 94-95 -11.85804481068696 1105 96-97 -12.197454259825742 1105 98-99 -11.650195857749555 1105 100-101 -11.228112151695 1106 1 0.14542136394153715 1106 2 0.03084956190068766 1106 3 0.09416585695048951 1106 4 0.12063648498094182 1106 5 -0.0397832506962672 1106 6 -0.09155028002667365 1106 7 0.08901392109480355 1106 8 0.16533029449829684 1106 9 0.0848753341687214 1106 10-11 0.054165161510773885 1106 12-13 0.12276481528193273 1106 14-15 0.2003966809496589 1106 16-17 0.1645846145787928 1106 18-19 0.1787080275933164 1106 20-21 0.11948573201753021 1106 22-23 0.1944136861436334 1106 24-25 0.14039569289494125 1106 26-27 0.1477009559676219 1106 28-29 0.1942181771309066 1106 30-31 0.2729228384147717 1106 32-33 0.47836339111654524 1106 34-35 0.1861951143163907 1106 36-37 0.23641246435479957 1106 38-39 0.23525910867715538 1106 40-41 0.20693916702798987 1106 42-43 0.17732843263363662 1106 44-45 0.167749944405287 1106 46-47 -0.06589814694387286 1106 48-49 0.06897424552670373 1106 50-51 0.23702977710169648 1106 52-53 0.018983658779688994 1106 54-55 -0.27495407800178384 1106 56-57 0.8769043282929161 1106 58-59 -6.952688392647659 1106 60-61 -9.133004132633456 1106 62-63 -2.8903087122958624 1106 64-65 -0.3397396466108056 1106 66-67 -1.5546215493143727 1106 68-69 -4.162775265632559 1106 70-71 -4.884871835344285 1106 72-73 -6.447236391270788 1106 74-75 -6.91149356235389 1106 76-77 -8.505300728647342 1106 78-79 -12.080499002664121 1106 80-81 -8.221977351163009 1106 82-83 -4.3461728422352195 1106 84-85 -3.4413617188123276 1106 86-87 -3.3677087483506085 1106 88-89 -3.4322767770850824 1106 90-91 -8.839465277820283 1106 92-93 -12.218545739700453 1106 94-95 -11.995304986408675 1106 96-97 -12.422960804563056 1106 98-99 -11.773447606001305 1106 100-101 -11.510732736238662 1107 1 0.07162836365280967 1107 2 0.20980172717764134 1107 3 0.07534087060413697 1107 4 -0.02343678900686541 1107 5 0.09170605771611662 1107 6 0.04365769022403043 1107 7 0.24757536598103513 1107 8 0.009962585284434056 1107 9 -0.07099677725894082 1107 10-11 0.14103296290848988 1107 12-13 0.08061383552411172 1107 14-15 0.1626126068407956 1107 16-17 0.11314341570169262 1107 18-19 0.04358632751435465 1107 20-21 0.12244361617199218 1107 22-23 0.11738555441465337 1107 24-25 0.27674239462625394 1107 26-27 0.22797152427237677 1107 28-29 0.2576544085158474 1107 30-31 0.20051346557804806 1107 32-33 0.20000694224727056 1107 34-35 0.14276834482124912 1107 36-37 0.07477590528235112 1107 38-39 0.2334138662336258 1107 40-41 0.010620085666168677 1107 42-43 0.2955949239770561 1107 44-45 0.3074135255218664 1107 46-47 0.23971480610497764 1107 48-49 0.06643449341429886 1107 50-51 0.1529220994042788 1107 52-53 0.061606341573309464 1107 54-55 -0.024743794458167656 1107 56-57 0.4160749866943476 1107 58-59 -6.858410748797368 1107 60-61 -9.288195475004283 1107 62-63 -3.040262620369429 1107 64-65 -0.6970051256711542 1107 66-67 -1.9065502790208804 1107 68-69 -4.128902743725849 1107 70-71 -4.932332952890871 1107 72-73 -6.269065179766244 1107 74-75 -6.749207715880008 1107 76-77 -9.332968929392466 1107 78-79 -12.588262100598158 1107 80-81 -8.385582992119076 1107 82-83 -4.355049625470976 1107 84-85 -3.406883218521859 1107 86-87 -3.214204718350853 1107 88-89 -3.088042790116056 1107 90-91 -8.475108773292838 1107 92-93 -11.75174688486741 1107 94-95 -11.799629974494346 1107 96-97 -12.588583706510743 1107 98-99 -11.83403413881006 1107 100-101 -11.242095596234002 1108 1 -0.04282494920330748 1108 2 -0.01881229712488519 1108 3 -0.0716109822979547 1108 4 -0.007633870466555948 1108 5 -0.12300280510242345 1108 6 -0.05129122298642841 1108 7 0.01786665092864581 1108 8 -0.041909929404866375 1108 9 -0.11814150773469834 1108 10-11 0.06443623970233858 1108 12-13 -0.03707396854138523 1108 14-15 0.004638131551082836 1108 16-17 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2312 18-19 -0.47951553328042706 2312 20-21 -0.29302969406515444 2312 22-23 -0.2504313329387742 2312 24-25 -0.3084832002510325 2312 26-27 -0.384806424962882 2312 28-29 -0.6056068904707246 2312 30-31 -0.4421865284764479 2312 32-33 -0.1792161851224492 2312 34-35 -0.2952328753639648 2312 36-37 -0.33973465267484926 2312 38-39 -0.42724266051140347 2312 40-41 -0.2977363327522653 2312 42-43 -0.26023261989077895 2312 44-45 -0.5148902808589639 2312 46-47 -0.2564558944999078 2312 48-49 -0.1446923325770726 2312 50-51 -0.01128372062753158 2312 52-53 0.017126569485952814 2312 54-55 -0.09732729857055489 2312 56-57 1.0729367522426472 2312 58-59 4.4760044936003425 2312 60-61 4.291347599433184 2312 62-63 1.5694014635801032 2312 64-65 1.4944439318430867 2312 66-67 -0.1250111680883812 2312 68-69 -0.8323593759603973 2312 70-71 0.06830662053277337 2312 72-73 7.094249776778533 2312 74-75 5.781136805115885 2312 76-77 5.924238760882787 2312 78-79 5.946182968000869 2312 80-81 3.3367841671856517 2312 82-83 1.8261885837431713 2312 84-85 1.4784114774223447 2312 86-87 1.1601772637618133 2312 88-89 1.4383261375444256 2312 90-91 3.2247546528026945 2312 92-93 5.1211796822355 2312 94-95 9.152458883408848 2312 96-97 9.870934687916959 2312 98-99 10.318396869288408 2312 100-101 9.640321327680567 2313 1 -0.23717578806321882 2313 2 -0.23498734213250216 2313 3 -0.24063958713877298 2313 4 -0.32869333181130145 2313 5 -0.32628336027330107 2313 6 -0.2841819173090627 2313 7 -0.3992449628316308 2313 8 -0.3704424502930195 2313 9 -0.35083216820544294 2313 10-11 -0.6053129999125773 2313 12-13 -0.5022988527525598 2313 14-15 -0.6439818913245787 2313 16-17 -0.6521062346054336 2313 18-19 -0.4849161760839351 2313 20-21 -0.6121344210880295 2313 22-23 -0.5561330654641239 2313 24-25 -0.6025679502013261 2313 26-27 -0.6963326375796726 2313 28-29 -0.5803313605044025 2313 30-31 -0.810940243193663 2313 32-33 -0.6743772942059252 2313 34-35 -0.7244294495796169 2313 36-37 -0.8053250495312483 2313 38-39 -0.9745571041894507 2313 40-41 -1.1930430739356908 2313 42-43 -0.8531917937751814 2313 44-45 -0.8776601633832399 2313 46-47 -0.7872267495722127 2313 48-49 -0.8710566375634201 2313 50-51 -0.5458859040014232 2313 52-53 -0.6525678764323288 2313 54-55 -0.5804817984720358 2313 56-57 0.3961272885857241 2313 58-59 3.9647764230488036 2313 60-61 3.7172171625922665 2313 62-63 1.084451036911279 2313 64-65 0.7876083876779916 2313 66-67 1.5688090430817354 2313 68-69 0.16503185863787095 2313 70-71 2.3510905910690703 2313 72-73 7.991988155999735 2313 74-75 9.52493122875702 2313 76-77 8.561667085933202 2313 78-79 6.051777227180633 2313 80-81 3.0696664554675372 2313 82-83 1.7501927268978115 2313 84-85 1.3886427398393444 2313 86-87 1.1336738115200156 2313 88-89 1.0177671796785894 2313 90-91 2.726334596732908 2313 92-93 4.677382025104411 2313 94-95 8.574886030289857 2313 96-97 9.185828910813749 2313 98-99 9.615072877346764 2313 100-101 8.677576401982058 2314 1 -0.4298739224017396 2314 2 -0.38290243568061655 2314 3 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1.2354280109340117 2314 64-65 1.2320710195206317 2314 66-67 1.8277875400934285 2314 68-69 -0.24508272928229502 2314 70-71 2.4152242009970877 2314 72-73 8.068405845410517 2314 74-75 9.716041301632735 2314 76-77 8.68546212510557 2314 78-79 6.276774352840565 2314 80-81 3.33355363071966 2314 82-83 1.887578596404417 2314 84-85 1.4651540166659878 2314 86-87 1.3136026558378937 2314 88-89 1.4461277493261768 2314 90-91 2.816973095049704 2314 92-93 4.895837353838434 2314 94-95 8.992079446049576 2314 96-97 9.471180597516716 2314 98-99 9.646285194474304 2314 100-101 8.825236049409746 2315 1 -0.8033401133080034 2315 2 -0.6524586065003177 2315 3 -0.6404352448654649 2315 4 -0.4171394791080232 2315 5 -0.6862322747049703 2315 6 -0.7920916661383473 2315 7 -0.797848623964029 2315 8 -0.9177221437796064 2315 9 -1.227273206945334 2315 10-11 -0.9449724294570601 2315 12-13 -0.8365006407474525 2315 14-15 -0.8888946201453578 2315 16-17 -1.0674829906718415 2315 18-19 -1.066218858076276 2315 20-21 -1.0603591975886744 2315 22-23 -0.905149668273836 2315 24-25 -1.0175360217211278 2315 26-27 -1.0018073076520437 2315 28-29 -0.957487597200867 2315 30-31 -1.0165085701413048 2315 32-33 -0.9936280392037986 2315 34-35 -0.9553277041560335 2315 36-37 -1.1470875016385307 2315 38-39 -1.1932799649812793 2315 40-41 -0.9675769181247063 2315 42-43 -1.0045072471596015 2315 44-45 -1.2376686776446348 2315 46-47 -1.184493671666715 2315 48-49 -1.1667313927784022 2315 50-51 -1.2585636392078996 2315 52-53 -1.3370634064450968 2315 54-55 -1.3214439100088597 2315 56-57 0.15910728007146702 2315 58-59 3.671434575454086 2315 60-61 3.170839980812783 2315 62-63 0.7896191935736887 2315 64-65 0.47344065672864843 2315 66-67 1.2334067442311643 2315 68-69 5.715231943780488 2315 70-71 4.6607202206987 2315 72-73 8.357343626412678 2315 74-75 9.409128759621218 2315 76-77 8.216941670862962 2315 78-79 5.592356196955002 2315 80-81 2.673625587012875 2315 82-83 0.9684473033133045 2315 84-85 0.39528386372185054 2315 86-87 0.4376840286336794 2315 88-89 0.5321903384695652 2315 90-91 1.873205605672883 2315 92-93 3.8083739365560945 2315 94-95 7.927138052426937 2315 96-97 8.826050281609833 2315 98-99 9.157524984626457 2315 100-101 8.305426550981633 2316 1 -0.5354668711581283 2316 2 -0.47545160720666857 2316 3 -0.5532336780511073 2316 4 -0.40278643216780097 2316 5 -0.6488573308919641 2316 6 -0.6337946135473231 2316 7 -0.6734897977905234 2316 8 -0.5975128071071651 2316 9 -0.7519460381275067 2316 10-11 -0.6985622965346963 2316 12-13 -0.7393080638515457 2316 14-15 -0.9800877107508938 2316 16-17 -1.0203941189066015 2316 18-19 -1.047406811585553 2316 20-21 -0.8670191218165044 2316 22-23 -0.9337132077331489 2316 24-25 -2.2433020912003485 2316 26-27 -1.546901701795754 2316 28-29 -1.3355133468958513 2316 30-31 -0.7781225815713455 2316 32-33 -0.7680314939010344 2316 34-35 -0.7843259703860497 2316 36-37 -0.6699170784702275 2316 38-39 -0.9660719767966057 2316 40-41 -0.7583118439578769 2316 42-43 -0.9514824606701708 2316 44-45 -0.8443953841237928 2316 46-47 -0.7413799491983468 2316 48-49 -0.9250419302470974 2316 50-51 -0.8223175722972229 2316 52-53 -0.7010501142085346 2316 54-55 -0.9006810512147965 2316 56-57 0.33827282299832717 2316 58-59 3.866667992265235 2316 60-61 3.7435382406214224 2316 62-63 1.1765064343108627 2316 64-65 0.9378958034569607 2316 66-67 5.939161576312326 2316 68-69 8.825762027900439 2316 70-71 5.451679573424574 2316 72-73 9.225829788359636 2316 74-75 10.239390430461775 2316 76-77 8.684282260344759 2316 78-79 6.091737176856114 2316 80-81 3.1876305956817284 2316 82-83 1.6208354109354737 2316 84-85 1.323969730285775 2316 86-87 1.4212299093246195 2316 88-89 1.2971434624662272 2316 90-91 2.9772077247250905 2316 92-93 4.799478733319724 2316 94-95 8.79639060492164 2316 96-97 9.504250692577534 2316 98-99 9.96443020520053 2316 100-101 8.682657013962434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 17.0 4 40.0 5 107.0 6 234.0 7 569.0 8 1255.0 9 2229.0 10 3625.0 11 5375.0 12 7518.0 13 10211.0 14 13412.0 15 18500.0 16 24433.0 17 32263.0 18 35350.0 19 36861.0 20 38601.0 21 38479.0 22 36603.0 23 35312.0 24 36537.0 25 40080.0 26 52936.0 27 76211.0 28 105853.0 29 138163.0 30 165109.0 31 177938.0 32 185024.0 33 192262.0 34 194410.0 35 186367.0 36 150798.0 37 88488.0 38 25753.0 39 997.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.95492826399517 19.16607879326663 14.051811963324115 23.827180979414077 2 16.222865881916768 20.814023542496987 38.47020113078135 24.492909444804894 3 19.766999451768868 24.439430191825284 29.33124606958133 26.46232428682452 4 13.491497839124861 16.429509500343386 36.12591187262561 33.953080787906146 5 13.924756735702642 37.6665175124791 33.71586359115369 14.692862160664562 6 33.025259034042485 35.72466276568975 16.92406719245051 14.326011007817247 7 29.46302044281489 32.124979494161515 20.211388548798332 18.20061151422526 8 26.994269020969718 34.63087851686878 19.369476454420713 19.005376007740786 9 26.707221113645442 16.27347976432883 19.55951141885573 37.459787703169994 10-11 25.542512658010807 26.185191123682877 26.96642881438717 21.30586740391914 12-13 26.235192930977114 23.35844391039157 27.47654919871988 22.92981395991143 14-15 23.058101538031636 24.82422313518964 25.467434968063003 26.650240358715717 16-17 22.676700084618442 28.07633918186107 26.16869377113723 23.078266962383257 18-19 22.658388224815493 26.78975426967219 28.19726084784517 22.35459665766715 20-21 23.47283636757955 25.892594820387394 28.092604830017027 22.541963982016032 22-23 23.100070345452707 25.808045888590968 28.139107901027003 22.95277586492932 24-25 22.900642377249966 26.079093683645656 28.248124816374272 22.77213912273011 26-27 22.713340954873654 26.232425776871143 28.147233515252424 22.906999753002776 28-29 22.652490699773274 26.527085063911183 28.122842932464888 22.697581303850658 30-31 22.781691560549625 26.18415680342429 27.967581180289365 23.066570455736716 32-33 22.58034319710072 26.221358631215463 27.97569134458293 23.22260682710089 34-35 23.12688435341364 26.2834920214171 27.897026834397487 22.692596790771777 36-37 23.22945853930996 25.932057263239866 27.965727343730084 22.872756853720094 38-39 22.784502975410366 26.232650971629024 27.97124453662187 23.011601516338743 40-41 22.975436228451628 26.141098167711874 28.04868580450077 22.83477979933572 42-43 22.83355528557675 26.070388449645428 27.785879202047308 23.310177062730517 44-45 22.683764122598106 26.20197945820768 27.98557309449799 23.128683324696226 46-47 23.045709091431625 26.41100033050312 27.506620492027828 23.036670086037425 48-49 22.769171293741472 26.47151871963392 27.496053553470613 23.263256433153998 50-51 22.781721003769768 26.612974622084547 27.468461273544833 23.136843100600853 52-53 23.049359154856138 26.371603277400606 27.314012382372677 23.265025185370586 54-55 22.54652685244192 26.486391286715715 27.53652743178263 23.43055442905973 56-57 22.593963414775644 26.570303453621047 27.43200313733498 23.40372999426833 58-59 20.986524860131254 26.07640661691168 29.351626550794883 23.585441972162183 60-61 22.456820957917504 26.362058926419262 27.841018840601606 23.340101275061624 62-63 22.405050620669137 26.37198533953871 27.697849727954743 23.52511431183741 64-65 21.6373154822808 26.42407218704247 28.064840795342178 23.873771535334555 66-67 21.615027338467872 26.149003468751836 28.611088247398435 23.624880945381857 68-69 22.18053367748039 25.39553983698473 29.164269581966707 23.259656903568175 70-71 21.29664599530621 25.706267530885533 29.272343929331875 23.724742544476378 72-73 21.161373529089985 25.897160885815158 29.192750737301065 23.748714847793792 74-75 21.736058379857216 26.154822311114295 28.40132289238805 23.707796416640445 76-77 20.297851351883562 26.306756698467233 29.10818537974078 24.287206569908424 78-79 21.502963947846805 26.51357299834319 27.796550132738222 24.186912921071784 80-81 22.208309631855712 26.518437335996392 26.980248318112253 24.29300471403564 82-83 22.396587822150796 26.4920168421599 26.90030390404084 24.21109143164847 84-85 22.247264323563208 26.535001389068135 26.753498679168818 24.464235608199836 86-87 22.32671861003224 26.372364695997845 26.83958620171902 24.461330492250898 88-89 22.256717864852824 26.370607086495035 26.625435633316595 24.747239415335546 90-91 21.868168236866584 26.344660526765008 27.036438779621967 24.75073245674644 92-93 21.74123371046291 26.29176551423173 27.11630508962437 24.850695685680993 94-95 20.238972915322538 26.10730012605335 28.55069648762801 25.103030470996103 96-97 19.97326240253728 25.861075802004642 28.848637159812444 25.317024635645634 98-99 20.386270516757186 26.05611769841374 28.052549688911537 25.505062095917534 100-101 20.29815286238848 26.020230554792832 28.11658514564417 25.565031437174518 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 218.0 1 161.5 2 114.0 3 145.0 4 168.5 5 175.5 6 256.5 7 406.5 8 579.0 9 729.5 10 791.0 11 905.0 12 1126.0 13 1283.0 14 1428.5 15 1586.0 16 1696.5 17 1892.5 18 2177.5 19 2434.0 20 2781.0 21 3241.0 22 3885.5 23 4748.5 24 5717.0 25 7016.0 26 8732.0 27 10851.0 28 13464.0 29 16393.5 30 19753.5 31 24095.0 32 29256.0 33 33904.5 34 38018.5 35 42604.5 36 47525.5 37 52149.0 38 56017.5 39 59387.0 40 62789.5 41 66002.0 42 69346.0 43 72880.0 44 76317.5 45 79538.0 46 82698.0 47 85640.0 48 87811.0 49 88781.0 50 88067.0 51 86532.5 52 85040.5 53 83675.0 54 80711.5 55 75800.0 56 69449.5 57 62071.5 58 54364.5 59 46260.0 60 38100.5 61 30383.0 62 23465.0 63 17498.0 64 12828.5 65 9416.5 66 6912.5 67 5079.0 68 3665.5 69 2608.0 70 1914.0 71 1359.0 72 976.5 73 755.5 74 536.5 75 364.0 76 242.5 77 133.0 78 77.5 79 46.0 80 31.0 81 20.0 82 14.0 83 8.5 84 4.5 85 2.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019833895757121897 2 0.005653587108338485 3 0.0033828840894156507 4 0.0 5 0.06103093624329331 6 4.63408779372007E-5 7 0.0 8 4.63408779372007E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 6.951131690580105E-5 16-17 0.0 18-19 0.0012975445822416196 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.390226338116021E-4 28-29 0.002062169068205431 30-31 0.007553563103763713 32-33 3.475565845290052E-4 34-35 9.26817558744014E-4 36-37 0.02395823389353276 38-39 0.02263751887232254 40-41 0.024908221891245374 42-43 0.025533823743397584 44-45 0.02613625515658119 46-47 0.028036231152006423 48-49 0.027874038079226216 50-51 0.022938734578914346 52-53 0.019439998294655692 54-55 0.013763240747348608 56-57 0.14954201310334667 58-59 0.7954411697920499 60-61 0.3174118434308562 62-63 4.1706790143480627E-4 64-65 0.15535779328446533 66-67 1.4735008957691704 68-69 2.815509550391534 70-71 2.2691042586340004 72-73 1.9429571597119821 74-75 1.62183804604615 76-77 1.1606536288151286 78-79 0.4411883284011192 80-81 2.0853395071740314E-4 82-83 1.853635117488028E-4 84-85 2.317043896860035E-5 86-87 0.0027341117982948413 88-89 2.317043896860035E-5 90-91 0.23469337631295295 92-93 0.18221233204907314 94-95 1.346735424171958 96-97 1.3304697760160005 98-99 1.0583329703297895 100-101 0.42596534999874874 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2157922.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.16898890242638 #Duplication Level Percentage of deduplicated Percentage of total 1 72.52032082296087 35.65750849744564 2 14.192613158140489 13.956728777380805 3 5.302786160668217 7.821979016575072 4 2.49446164188221 4.906006271489386 5 1.4406445875176108 3.5417518867997018 6 0.8672581860513834 2.5585324875299342 7 0.6025180908980082 2.0737643727412705 8 0.43133713800562373 1.6966728761442296 9 0.3051839548730065 1.35050278413145 >10 1.6543066268806712 14.857029785734662 >50 0.12610806234530994 4.242567872507669 >100 0.058117109373578155 5.066855789284931 >500 0.0029277885324372104 0.9506230653152284 >1k 0.001322227079165178 1.0510674837743492 >5k 9.444479136894128E-5 0.2684090331455217 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5780 0.26785027447702003 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4157 0.19263902958494328 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3020 0.13994945137034612 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.268175587440139E-5 2 0.0 0.0 0.0 0.0 1.390226338116021E-4 3 0.0 0.0 0.0 0.0 1.390226338116021E-4 4 0.0 0.0 0.0 0.0 1.390226338116021E-4 5 0.0 0.0 0.0 0.0 1.390226338116021E-4 6 4.6340877937200695E-5 0.0 0.0 0.0 1.390226338116021E-4 7 1.8536351174880278E-4 0.0 0.0 0.0 1.390226338116021E-4 8 1.8536351174880278E-4 0.0 0.0 0.0 1.390226338116021E-4 9 1.8536351174880278E-4 0.0 0.0 0.0 1.390226338116021E-4 10-11 1.8536351174880278E-4 0.0 0.0 0.0 1.390226338116021E-4 12-13 2.548748286546038E-4 0.0 0.0 4.6340877937200695E-5 1.6219307278020242E-4 14-15 2.780452676232042E-4 0.0 0.0 4.6340877937200695E-5 3.0121570659180457E-4 16-17 3.2438614556040484E-4 0.0 0.0 4.6340877937200695E-5 3.243861455604049E-4 18-19 3.7072702349760556E-4 0.0 0.0 9.268175587440139E-5 3.7072702349760556E-4 20-21 3.7072702349760556E-4 0.0 0.0 3.475565845290052E-4 3.7072702349760556E-4 22-23 3.7072702349760556E-4 0.0 0.0 7.414540469952111E-4 3.7072702349760556E-4 24-25 6.02431413183609E-4 0.0 0.0 0.0013670558991474206 3.7072702349760556E-4 26-27 6.02431413183609E-4 0.0 0.0 0.0027109413593262408 3.7072702349760556E-4 28-29 9.268175587440139E-4 0.0 0.0 0.010055970512372552 3.7072702349760556E-4 30-31 0.001065840192555616 0.0 0.0 0.030863024706175662 3.938974624662059E-4 32-33 0.001065840192555616 0.0 0.0 0.0800770370754828 4.1706790143480627E-4 34-35 0.0011121810704928167 0.0 0.0 0.15732728059679635 5.097496573092077E-4 36-37 0.0011585219484300175 0.0 0.0 0.2800610958134724 5.097496573092077E-4 38-39 0.0014365672160532217 0.0 0.0 0.4687379803347851 5.097496573092077E-4 40-41 0.0014365672160532217 0.0 0.0 0.6839681879141136 5.560905352464084E-4 42-43 0.0014829080939904224 0.0 0.0 0.8967191585238021 5.560905352464084E-4 44-45 0.0015755898498648238 0.0 0.0 1.1446660259267945 5.560905352464084E-4 46-47 0.0017146124836764256 0.0 0.0 1.4285039032921487 5.560905352464084E-4 48-49 0.0017609533616136264 0.0 0.0 1.7041162748236498 5.560905352464084E-4 50-51 0.0017609533616136264 2.3170438968600347E-5 0.0 1.9851273586348348 5.560905352464084E-4 52-53 0.0017609533616136264 4.6340877937200695E-5 0.0 2.2513093615061157 5.792609742150086E-4 54-55 0.0017609533616136264 4.6340877937200695E-5 0.0 2.5303046171270323 6.487722911208098E-4 56-57 0.0018536351174880277 4.6340877937200695E-5 0.0 2.777255155654375 6.487722911208098E-4 58-59 0.0018536351174880277 4.6340877937200695E-5 0.0 2.9455883947612564 6.487722911208098E-4 60-61 0.0018536351174880277 4.6340877937200695E-5 0.0 3.069503902365331 6.487722911208098E-4 62-63 0.0018536351174880277 4.6340877937200695E-5 0.0 3.172357480946948 6.719427300894102E-4 64-65 0.0018536351174880277 4.6340877937200695E-5 0.0 3.281768293756679 6.951131690580105E-4 66-67 0.0018536351174880277 4.6340877937200695E-5 0.0 3.372272028368032 7.646244859638115E-4 68-69 0.0018536351174880277 4.6340877937200695E-5 0.0 3.477141435139917 7.877949249324119E-4 70-71 0.0018768055564566282 4.6340877937200695E-5 0.0 3.6675792730228434 7.877949249324119E-4 72-73 0.0019231464343938288 4.6340877937200695E-5 0.0 3.9039872618194726 7.877949249324119E-4 74-75 0.0019463168733624293 4.6340877937200695E-5 0.0 4.182495938222049 7.877949249324119E-4 76-77 0.0019463168733624293 4.6340877937200695E-5 0.0 4.58922982387686 7.877949249324119E-4 78-79 0.0019694873123310298 4.6340877937200695E-5 0.0 5.169255422577832 7.877949249324119E-4 80-81 0.002038998629236831 4.6340877937200695E-5 0.0 5.810057082693444 8.341358028696125E-4 82-83 0.002131680385111232 4.6340877937200695E-5 0.0 6.408781225642076 9.268175587440139E-4 84-85 0.002131680385111232 4.6340877937200695E-5 0.0 6.88813126702448 9.268175587440139E-4 86-87 0.002131680385111232 4.6340877937200695E-5 0.0 7.315301479849596 9.731584366812146E-4 88-89 0.002131680385111232 4.6340877937200695E-5 0.0 7.7476387005647105 9.731584366812146E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8585 0.0 34.00146 1 GGTATCA 5350 0.0 33.84735 1 ATCAACG 10240 0.0 28.314022 3 TATCAAC 10450 0.0 27.881664 2 TCAACGC 10410 0.0 27.805065 4 CAACGCA 10435 0.0 27.783623 5 AACGCAG 10690 0.0 27.106422 6 GTACATG 9940 0.0 26.472504 1 TACATGG 9740 0.0 26.380564 2 ACATGGG 10220 0.0 24.07942 3 ACGCAGA 12140 0.0 23.674776 7 CGCAGAG 12400 0.0 23.064913 8 GAGTACT 5915 0.0 20.990158 12-13 GCAGAGT 13405 0.0 20.808449 9 CATGGGA 4685 0.0 20.42644 4 TATAGGG 670 0.0 20.406557 2 CATGGGG 5225 0.0 19.668736 4 AGAGTAC 10700 0.0 19.551678 10-11 GTACTTT 6265 0.0 19.51669 14-15 AGTACTT 6195 0.0 19.052689 12-13 >>END_MODULE