FastQCFastQC Report
Fri 27 May 2016
SRR939774_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939774_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2337788
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53460.22867770730280074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38260.16365898019837555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26070.11151567207976086No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93100.030.8249341
TACATGG98450.028.241892
GTACATG103200.027.6258051
GGTATCA61950.027.3390351
ACATGGG106050.025.3307153
ATCAACG113500.025.2431453
CAACGCA113800.025.094995
TCAACGC114300.025.0674
TATCAAC116100.024.6778352
AACGCAG117200.024.3273586
CATGGGA50000.021.7356834
GTATAGG6600.021.3845121
CATGGGG56100.021.133384
ACGCAGA133100.021.1030967
CGCAGAG136400.020.5235528
AGAGTAC104200.019.91077810-11
GAGTACT57500.019.88159212-13
GAGTACA78150.019.1434481
AGTACTT62000.019.12148312-13
GCAGAGT139650.018.9341289
Produced by FastQC (version 0.11.5)