##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939774_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2337788 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.113002975462276 31.0 30.0 33.0 26.0 34.0 2 30.16583753531116 31.0 30.0 33.0 26.0 34.0 3 30.133121566198476 31.0 30.0 34.0 26.0 34.0 4 33.77044796191956 35.0 35.0 37.0 28.0 37.0 5 33.63891037168469 35.0 33.0 37.0 28.0 37.0 6 33.627130004944846 35.0 33.0 37.0 28.0 37.0 7 33.606333422876666 35.0 33.0 37.0 28.0 37.0 8 33.45239089258735 35.0 33.0 37.0 27.0 37.0 9 34.77342342419415 37.0 34.0 39.0 27.0 39.0 10-11 34.55489826280227 37.0 33.5 39.0 27.0 39.0 12-13 34.4527752302604 37.0 33.0 39.0 27.0 39.0 14-15 35.06051061944026 38.0 33.0 40.0 26.0 41.0 16-17 34.855394928881495 38.0 33.0 40.0 25.5 41.0 18-19 34.874496746497115 38.0 33.5 40.0 25.0 41.0 20-21 34.969673255231015 38.0 34.0 40.0 25.0 41.0 22-23 34.879636220221855 38.0 33.5 40.0 25.0 41.0 24-25 34.68833337325711 38.0 33.0 40.0 25.0 41.0 26-27 34.600083283856364 38.0 33.0 40.0 24.0 41.0 28-29 34.49523117579524 38.0 33.0 40.0 24.0 41.0 30-31 34.31298004780588 38.0 33.0 40.0 23.5 41.0 32-33 34.021045107597445 38.0 33.0 40.0 23.0 41.0 34-35 33.767646809719274 38.0 32.0 40.0 21.0 41.0 36-37 33.54760290496829 38.0 32.0 40.0 19.0 41.0 38-39 33.266551115841125 37.0 31.0 40.0 18.0 41.0 40-41 32.983626188516666 37.0 31.0 40.0 17.0 41.0 42-43 32.658476303240505 37.0 31.0 40.0 15.0 41.0 44-45 32.267232956966154 37.0 30.0 40.0 13.5 41.0 46-47 31.889301767311665 36.5 30.0 39.0 9.0 40.5 48-49 31.54285995992793 36.0 30.0 39.0 8.5 40.0 50-51 30.786702643695662 35.5 28.5 38.5 8.0 40.0 52-53 30.89242951028921 35.5 29.0 38.5 7.0 40.0 54-55 30.969284426132738 36.0 29.0 39.0 4.0 40.0 56-57 30.721805185072384 35.0 28.5 39.0 2.0 40.0 58-59 30.174596242259778 35.0 28.0 38.5 2.0 40.0 60-61 29.67758197064918 34.5 27.0 38.0 2.0 40.0 62-63 29.2170213894502 34.0 26.0 38.0 2.0 40.0 64-65 28.66341131017868 34.0 26.0 37.5 2.0 39.5 66-67 28.125857220586298 33.5 25.5 37.0 2.0 39.0 68-69 27.569898339798137 33.0 24.5 36.0 2.0 39.0 70-71 26.999416328597803 32.5 23.0 36.0 2.0 39.0 72-73 26.49963833333048 32.0 22.0 35.0 2.0 38.0 74-75 25.90602590996275 31.5 20.5 35.0 2.0 37.5 76-77 25.263538438900362 31.0 19.0 35.0 2.0 37.0 78-79 24.603433673198765 31.0 16.5 34.0 2.0 36.0 80-81 23.91728655464054 30.0 9.0 34.0 2.0 36.0 82-83 23.16641372100464 30.0 4.5 34.0 2.0 35.0 84-85 22.4091249933698 29.0 2.0 34.0 2.0 35.0 86-87 21.615062400867828 29.0 2.0 34.0 2.0 35.0 88-89 20.735458476132138 27.5 2.0 33.0 2.0 35.0 90-91 19.761937780500197 26.0 2.0 33.0 2.0 34.0 92-93 18.773817386349833 25.0 2.0 32.0 2.0 34.0 94-95 17.392538373881635 21.5 2.0 32.0 2.0 34.0 96-97 14.849660448252793 2.0 2.0 31.0 2.0 34.0 98-99 13.464837487402622 2.0 2.0 30.0 2.0 34.0 100-101 12.18346060463994 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7795484666998931 1101 2 0.7555890005375119 1101 3 0.9534062766453779 1101 4 0.8264362488435282 1101 5 1.047574684977853 1101 6 0.9876796563259518 1101 7 1.0053994960795478 1101 8 0.6562761554983538 1101 9 0.7680787451232902 1101 10-11 1.0225057886490134 1101 12-13 0.8531112970135197 1101 14-15 1.0369171928094545 1101 16-17 1.0211446815844099 1101 18-19 0.9405631531727536 1101 20-21 0.9623255153830499 1101 22-23 1.0160144635443729 1101 24-25 1.08590580756222 1101 26-27 1.0433362570030482 1101 28-29 1.3087202523487562 1101 30-31 1.069297815320887 1101 32-33 1.0665509268303381 1101 34-35 0.9498985118833403 1101 36-37 0.9211907246575137 1101 38-39 1.0326087281565322 1101 40-41 1.0684825711545543 1101 42-43 0.9523544254313805 1101 44-45 0.8697364986147775 1101 46-47 0.8868403937320757 1101 48-49 0.9307377241957262 1101 50-51 0.9015288486790602 1101 52-53 1.0033300860927206 1101 54-55 0.9562059974074053 1101 56-57 0.6512111003852787 1101 58-59 0.5946146776181997 1101 60-61 0.7693939848776665 1101 62-63 0.5579082734285343 1101 64-65 0.5369381570185716 1101 66-67 0.5252542555271944 1101 68-69 0.5869566086732725 1101 70-71 0.5197408541956889 1101 72-73 0.2973551573629152 1101 74-75 0.2464297529716184 1101 76-77 -0.17415260651123887 1101 78-79 -0.049350281374682226 1101 80-81 -0.4279695166971855 1101 82-83 -0.4492268650436948 1101 84-85 -0.46824341008622383 1101 86-87 -0.5107652835952479 1101 88-89 -0.6951995898237655 1101 90-91 -0.8651658566576224 1101 92-93 -1.0122572086813122 1101 94-95 -0.9851524630036401 1101 96-97 0.8453777112314587 1101 98-99 0.9382440713459435 1101 100-101 0.07634165168224882 1102 1 0.9485044123501787 1102 2 0.9736517695512212 1102 3 0.9982203824208007 1102 4 1.059593179045649 1102 5 1.0751776641928714 1102 6 0.9908816223624939 1102 7 0.8983026460320005 1102 8 0.8756552386280632 1102 9 1.101586096034744 1102 10-11 1.1029392905046294 1102 12-13 0.8813740307551257 1102 14-15 1.0392648326454648 1102 16-17 1.0606612620303721 1102 18-19 1.1164767135085114 1102 20-21 0.9422814380297879 1102 22-23 0.8847677223802961 1102 24-25 0.9520034346184829 1102 26-27 0.9474210480797183 1102 28-29 1.047879511021094 1102 30-31 1.052450911598406 1102 32-33 1.2227175389700804 1102 34-35 1.0152423254625944 1102 36-37 0.7818077564841985 1102 38-39 0.8392252513492195 1102 40-41 0.9977041694410431 1102 42-43 0.8981066286600523 1102 44-45 0.8719001100695252 1102 46-47 0.7253583646146957 1102 48-49 0.6456291985697682 1102 50-51 0.752272885648317 1102 52-53 0.7038063234279903 1102 54-55 0.5732621056160951 1102 56-57 0.5304316123931265 1102 58-59 0.5333817897017035 1102 60-61 0.7142784431696789 1102 62-63 0.33622723806441357 1102 64-65 0.1458628306237948 1102 66-67 -0.11013088775934676 1102 68-69 -0.10836619499911748 1102 70-71 0.21365564905516266 1102 72-73 0.15813193604834197 1102 74-75 0.07075293748051692 1102 76-77 0.06487725720552362 1102 78-79 -0.15176015858685687 1102 80-81 -0.28975877097328606 1102 82-83 -0.3000798493706114 1102 84-85 -0.3619008847056442 1102 86-87 -0.428647091367381 1102 88-89 -0.5556766834021971 1102 90-91 -0.6522926240968694 1102 92-93 -0.5349367897100308 1102 94-95 -0.31184820785851386 1102 96-97 0.8156526547449694 1102 98-99 0.8113724771489697 1102 100-101 0.49034193028077055 1103 1 0.6828293319651486 1103 2 0.6784983328669298 1103 3 0.8680511251425607 1103 4 0.8218079852754272 1103 5 0.8495374044992303 1103 6 0.9135549437641401 1103 7 0.8338523155174897 1103 8 0.7542302569614918 1103 9 0.9943727257097663 1103 10-11 0.8627518408253962 1103 12-13 0.7160395290397332 1103 14-15 0.8820210775492825 1103 16-17 1.0304857867715285 1103 18-19 0.9030035033894777 1103 20-21 1.1040268187753313 1103 22-23 0.913008572333375 1103 24-25 0.9052852463200765 1103 26-27 1.1830693719172771 1103 28-29 1.0665453118713586 1103 30-31 1.1055226581201865 1103 32-33 1.113702236327498 1103 34-35 1.1076200427842195 1103 36-37 1.1100337986690292 1103 38-39 0.9350190118300432 1103 40-41 1.095298900696072 1103 42-43 1.0196969957901487 1103 44-45 1.046995559276958 1103 46-47 0.9125731067415792 1103 48-49 0.5284628925887098 1103 50-51 0.8996605004565978 1103 52-53 1.0616107224249092 1103 54-55 0.9339142756272274 1103 56-57 0.743745847337923 1103 58-59 0.5338571126520826 1103 60-61 0.715744155814356 1103 62-63 0.5960186344305498 1103 64-65 0.4702353274058275 1103 66-67 0.4126935882636076 1103 68-69 0.08863481526247341 1103 70-71 0.2470548587946979 1103 72-73 0.2694087887501482 1103 74-75 0.08663955741189966 1103 76-77 -0.005835925939006614 1103 78-79 0.026375690827755705 1103 80-81 -0.2598111814984705 1103 82-83 -0.03470220640833688 1103 84-85 -0.2816610501423895 1103 86-87 -0.3380594473281775 1103 88-89 -0.1648176277147293 1103 90-91 -0.3521898030567563 1103 92-93 -0.21042294604929523 1103 94-95 -0.1078962004123234 1103 96-97 0.9937683117483402 1103 98-99 1.0304303312918126 1103 100-101 0.8859564590732258 1104 1 0.6009866952781877 1104 2 0.5445591763977831 1104 3 0.7348202693545858 1104 4 0.9625827456588141 1104 5 0.9106019653528108 1104 6 0.9237775812548463 1104 7 0.7853588359024855 1104 8 0.9243153535114104 1104 9 1.0272923813048394 1104 10-11 0.9995480612124013 1104 12-13 0.88923976714932 1104 14-15 1.0023022126388028 1104 16-17 1.0853897643068464 1104 18-19 1.0444143529484222 1104 20-21 0.9209417674360907 1104 22-23 0.8000974062996491 1104 24-25 1.0313049983646039 1104 26-27 1.2443712888501821 1104 28-29 1.3307816532220471 1104 30-31 1.3010871134821684 1104 32-33 1.2944331925836536 1104 34-35 1.1437904719842962 1104 36-37 1.1315082445870104 1104 38-39 0.8765389949513818 1104 40-41 0.9597591605067848 1104 42-43 1.044334305654111 1104 44-45 1.137650511353634 1104 46-47 0.9246788947135336 1104 48-49 0.7554958662459548 1104 50-51 1.0651347353913927 1104 52-53 0.979061668835179 1104 54-55 0.9594583108312769 1104 56-57 1.0382676941440394 1104 58-59 0.9265910025791406 1104 60-61 1.2217747584225123 1104 62-63 1.0680445250809818 1104 64-65 1.0322587314687617 1104 66-67 0.9823720398479914 1104 68-69 0.9166307764415684 1104 70-71 1.244671251183231 1104 72-73 1.1339946337880242 1104 74-75 1.0011304600180466 1104 76-77 0.5784575064559583 1104 78-79 0.44692670857043737 1104 80-81 0.4357199937783207 1104 82-83 0.5115125292598748 1104 84-85 0.48741346208458936 1104 86-87 0.47358154971805533 1104 88-89 0.569617339231236 1104 90-91 0.4386988631276587 1104 92-93 0.4092553799498617 1104 94-95 0.6441326289324252 1104 96-97 2.0858335859260073 1104 98-99 2.262207286904969 1104 100-101 1.9224100829605408 1105 1 0.6785459955577409 1105 2 0.7682945861930577 1105 3 0.8610301423242745 1105 4 0.8628905777137206 1105 5 0.753822804621251 1105 6 0.8775519913624308 1105 7 0.8368289013854309 1105 8 0.8493371896237676 1105 9 0.9497200682257727 1105 10-11 0.8234325151699409 1105 12-13 0.6905169416833346 1105 14-15 0.8972777327064421 1105 16-17 0.8695325731164445 1105 18-19 0.8765881982370303 1105 20-21 0.8114727816981144 1105 22-23 0.7710641049587608 1105 24-25 0.7492290823269983 1105 26-27 1.0000232872353294 1105 28-29 0.7523914380855601 1105 30-31 0.9892233917707287 1105 32-33 0.9104316678644153 1105 34-35 1.0260347133444299 1105 36-37 0.988621437534448 1105 38-39 0.9900793206649325 1105 40-41 1.016993023365849 1105 42-43 0.9118014816607953 1105 44-45 0.9291069102232328 1105 46-47 0.9071378790042282 1105 48-49 0.893602770519994 1105 50-51 0.953646226645521 1105 52-53 0.8425113016837997 1105 54-55 0.8188348580848768 1105 56-57 0.7372650579412898 1105 58-59 0.5354557659493153 1105 60-61 0.7839846682089018 1105 62-63 0.9502171166974378 1105 64-65 0.9676085250413244 1105 66-67 0.935159540197116 1105 68-69 0.6208977686186543 1105 70-71 0.8567059007771043 1105 72-73 0.8114235997719348 1105 74-75 0.7726200669172378 1105 76-77 0.5487114369561787 1105 78-79 0.4853754051322632 1105 80-81 0.4846716064767804 1105 82-83 0.5154199397000774 1105 84-85 0.5535610926124903 1105 86-87 0.6216499107146944 1105 88-89 0.48660678680298375 1105 90-91 0.6221535487511183 1105 92-93 0.743029164808295 1105 94-95 0.9321991141816213 1105 96-97 2.195748736125452 1105 98-99 2.253580356249861 1105 100-101 1.7149993888514654 1106 1 0.956655495817273 1106 2 1.0528251242863753 1106 3 0.9374258398846855 1106 4 0.9777613170873849 1106 5 0.9444041890724861 1106 6 1.1887146881730857 1106 7 0.9068098691459667 1106 8 1.0511066832508575 1106 9 1.1644021072707034 1106 10-11 1.150268243153107 1106 12-13 1.081351458523983 1106 14-15 1.410919126385437 1106 16-17 1.5054144723032508 1106 18-19 1.200974606766934 1106 20-21 1.1819157678853287 1106 22-23 0.9523941268746725 1106 24-25 1.3359896906377386 1106 26-27 1.5267373602787515 1106 28-29 1.3210837646326468 1106 30-31 1.169314876285405 1106 32-33 1.2389946256474431 1106 34-35 1.3770325541945354 1106 36-37 1.412192487623841 1106 38-39 1.3473863669190393 1106 40-41 1.4180517818005924 1106 42-43 1.416317403138386 1106 44-45 1.5851951542107727 1106 46-47 1.3260280074718374 1106 48-49 1.1041439457607858 1106 50-51 1.3249379137382071 1106 52-53 1.4545712712610523 1106 54-55 1.4754792003191461 1106 56-57 1.2766745899212175 1106 58-59 1.2898575120131035 1106 60-61 1.5774154816929524 1106 62-63 1.3798117083693917 1106 64-65 1.5772006115226667 1106 66-67 1.4551806090303785 1106 68-69 1.3985083033957473 1106 70-71 1.5293859861338142 1106 72-73 1.6454613903019535 1106 74-75 1.4964752488769868 1106 76-77 1.5105835172376239 1106 78-79 1.1903847049765446 1106 80-81 1.4208376375339355 1106 82-83 1.536697839232918 1106 84-85 1.4950939989219663 1106 86-87 1.3546293933838243 1106 88-89 1.6543980557631315 1106 90-91 1.409266866272315 1106 92-93 1.6390650160684608 1106 94-95 1.9773873459135558 1106 96-97 3.188628678019448 1106 98-99 3.30068577971719 1106 100-101 2.9082015811635937 1107 1 0.556362804237331 1107 2 0.5650176503034032 1107 3 0.5449826043123132 1107 4 0.03336089135417808 1107 5 0.518342812535117 1107 6 0.48796965506050327 1107 7 0.5262398596852336 1107 8 0.535549682304783 1107 9 0.7149667950663456 1107 10-11 0.7160957620745734 1107 12-13 0.5461028185050623 1107 14-15 0.7137307394412531 1107 16-17 0.6104168374877332 1107 18-19 0.49764413136485075 1107 20-21 0.5233109310830599 1107 22-23 0.40627894239027995 1107 24-25 0.558883198206324 1107 26-27 0.7464408252740213 1107 28-29 0.6910163568748473 1107 30-31 0.6827018204670523 1107 32-33 0.7405172131592721 1107 34-35 0.6006503995114727 1107 36-37 0.45465789680076085 1107 38-39 0.605714772784701 1107 40-41 0.6315111597570748 1107 42-43 0.5423832143966649 1107 44-45 0.47974488221456113 1107 46-47 0.4653654601681474 1107 48-49 0.5430911430171683 1107 50-51 0.6456761469453944 1107 52-53 0.644562106171179 1107 54-55 0.7898890867902928 1107 56-57 0.47963993996851784 1107 58-59 0.4215421146621061 1107 60-61 0.5125355148055348 1107 62-63 0.34087190231451814 1107 64-65 0.3128956801130194 1107 66-67 0.3399384732687949 1107 68-69 0.050502449564145735 1107 70-71 0.24773390477146862 1107 72-73 0.17908840070876408 1107 74-75 0.29532222144084486 1107 76-77 0.4492247187513456 1107 78-79 0.3034700881364323 1107 80-81 0.10548315550933651 1107 82-83 0.09967087612979952 1107 84-85 0.03752097385100939 1107 86-87 0.04302816348789307 1107 88-89 0.28696812435348207 1107 90-91 0.4816489026961541 1107 92-93 0.515895077563254 1107 94-95 0.7867002598208437 1107 96-97 2.2056180938188774 1107 98-99 2.4233486817985526 1107 100-101 1.837229785988571 1108 1 0.47870847887210743 1108 2 0.4825791840651448 1108 3 0.556718614655221 1108 4 0.6869443601563461 1108 5 0.5952286477686499 1108 6 0.4967871758172393 1108 7 0.48096849470492486 1108 8 0.5157939626107009 1108 9 0.5082852268282423 1108 10-11 0.3623942580977939 1108 12-13 0.3438893350831762 1108 14-15 0.26610637632659717 1108 16-17 0.567106929351965 1108 18-19 0.5132491860797117 1108 20-21 0.3717615404200387 1108 22-23 0.26437488705354184 1108 24-25 0.39094048788414426 1108 26-27 0.2795854568897198 1108 28-29 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1.6516934755719213 2311 94-95 2.3374580622342727 2311 96-97 3.8522129525412225 2311 98-99 4.127061313905221 2311 100-101 3.792110438509953 2312 1 0.05242825843991028 2312 2 -0.464697010195561 2312 3 -0.6893090249155058 2312 4 -0.025168510622592066 2312 5 -1.668808451511385 2312 6 -0.4193255128319393 2312 7 0.13852798352739626 2312 8 0.23303268229368967 2312 9 0.4211030225614394 2312 10-11 0.3087829147252492 2312 12-13 0.33299845840434017 2312 14-15 0.6402136645524621 2312 16-17 -0.05798137598581121 2312 18-19 0.2182996994924693 2312 20-21 0.44482592880899574 2312 22-23 0.4673902383846027 2312 24-25 0.518173302147666 2312 26-27 0.7560965819825114 2312 28-29 0.8538233554438435 2312 30-31 0.5239496573335032 2312 32-33 -0.27891112333556833 2312 34-35 0.15131729635773183 2312 36-37 0.685586616003846 2312 38-39 0.7861045112119385 2312 40-41 1.0312318936694567 2312 42-43 0.9408175078285055 2312 44-45 0.8632577140337006 2312 46-47 0.46336386832372156 2312 48-49 0.9088194811759571 2312 50-51 0.8038469679377762 2312 52-53 0.47767600624550255 2312 54-55 0.5682510955093818 2312 56-57 0.6653269289975512 2312 58-59 0.990187285882449 2312 60-61 0.9830635135187507 2312 62-63 1.0784858828130375 2312 64-65 0.8653007102304962 2312 66-67 1.134988502665223 2312 68-69 0.9491360702059879 2312 70-71 1.3883594247554925 2312 72-73 1.2411765733601001 2312 74-75 1.383039020114122 2312 76-77 1.3527347495898638 2312 78-79 1.0923274544381414 2312 80-81 1.1358124371271359 2312 82-83 1.1645842953228964 2312 84-85 1.110330523808301 2312 86-87 1.4693682663199326 2312 88-89 1.5138392348729646 2312 90-91 1.901532883860252 2312 92-93 1.8130323228404173 2312 94-95 2.045551081256942 2312 96-97 3.7244966338701317 2312 98-99 4.160263803766005 2312 100-101 3.3695870053081283 2313 1 -0.006606597021569627 2313 2 -0.5279269801420767 2313 3 -0.9356832244071285 2313 4 -0.08273108867081902 2313 5 -0.11310579933790876 2313 6 0.16501699389468882 2313 7 0.1247310598254856 2313 8 0.2838968808841358 2313 9 0.5064872451260101 2313 10-11 -0.49158796186092957 2313 12-13 0.16680097511542158 2313 14-15 0.229905112111922 2313 16-17 -0.266946604920129 2313 18-19 -0.0707728694438785 2313 20-21 -2.2949646838043165 2313 22-23 -0.24479615615570083 2313 24-25 0.05801508864920635 2313 26-27 0.052227821728060064 2313 28-29 -0.1646480579853815 2313 30-31 0.09273884237055796 2313 32-33 0.25097499956473257 2313 34-35 0.12725233734690988 2313 36-37 -0.7009954546111175 2313 38-39 0.1529123977839859 2313 40-41 0.3182784272196457 2313 42-43 0.24320547956879324 2313 44-45 0.22395495200265003 2313 46-47 0.05442692628360035 2313 48-49 -0.0502267462606909 2313 50-51 0.0526647116520742 2313 52-53 0.06940082018139293 2313 54-55 0.07639493168428046 2313 56-57 -0.0031873260236388035 2313 58-59 0.020517738315408707 2313 60-61 -0.11829064554871849 2313 62-63 0.14238833701401532 2313 64-65 -0.027006148572485955 2313 66-67 0.32468717393217617 2313 68-69 -0.028576110109195696 2313 70-71 0.308597892928983 2313 72-73 0.16732685937600422 2313 74-75 0.08366873429848098 2313 76-77 0.320886066950937 2313 78-79 0.08024151234509702 2313 80-81 0.07921665577231352 2313 82-83 0.2167560922523215 2313 84-85 0.2907435124635036 2313 86-87 0.34071957881752013 2313 88-89 0.5873043400744642 2313 90-91 0.9845697590989566 2313 92-93 1.1178030452254681 2313 94-95 1.3512825209776018 2313 96-97 2.8000591175095053 2313 98-99 2.9198221634551427 2313 100-101 2.5066806360007305 2314 1 -0.16367052638265278 2314 2 -0.2616518605374054 2314 3 -1.1980783617959858 2314 4 -0.22184438340176627 2314 5 -0.4245045497961968 2314 6 -0.5204600264888981 2314 7 -0.34269470880461483 2314 8 -1.8388492618137384 2314 9 -1.492907327697317 2314 10-11 -0.7521643810159659 2314 12-13 -0.5923059242052275 2314 14-15 -0.5705899511261663 2314 16-17 -0.7578670716576212 2314 18-19 -1.8925992126862212 2314 20-21 -1.4760033419227767 2314 22-23 -1.9836486111160454 2314 24-25 -1.8539586114442912 2314 26-27 -1.777797280069926 2314 28-29 -2.920813106055924 2314 30-31 -1.5636894821743965 2314 32-33 -1.4622215884501344 2314 34-35 -1.7231303001262965 2314 36-37 -3.1807651898501277 2314 38-39 -3.6735659879034834 2314 40-41 -4.398283623630238 2314 42-43 -3.0914649168791684 2314 44-45 -3.593857390314497 2314 46-47 -3.4349482953627266 2314 48-49 -1.731592485005553 2314 50-51 -1.1915205633093287 2314 52-53 -2.1440731771899664 2314 54-55 -1.7674837684504503 2314 56-57 -1.886297707206591 2314 58-59 -3.930903572271358 2314 60-61 -2.4433675516696454 2314 62-63 -2.0547874857858837 2314 64-65 -1.3721701162460498 2314 66-67 -2.216204652163647 2314 68-69 -2.008065249532887 2314 70-71 -1.7653525062780773 2314 72-73 -1.1801900269813785 2314 74-75 -1.5718773160295747 2314 76-77 -0.7395168214052923 2314 78-79 -0.5221568414927873 2314 80-81 -0.3382514365285232 2314 82-83 -0.2857106017554152 2314 84-85 -0.11654665547412435 2314 86-87 0.1834054187193317 2314 88-89 0.2849302033236505 2314 90-91 0.6949821318561611 2314 92-93 0.929773109007126 2314 94-95 1.4642014372217211 2314 96-97 2.960138186673653 2314 98-99 2.9823753492017016 2314 100-101 2.518640915479411 2315 1 -2.405247665985083 2315 2 -2.0240821977822385 2315 3 -1.7242485072735327 2315 4 -1.6424799783555244 2315 5 -1.7960576004820439 2315 6 -2.962719003583185 2315 7 -2.1713007299323728 2315 8 -1.3922464913523207 2315 9 -1.2299479311317612 2315 10-11 -3.5665784350343017 2315 12-13 -1.8760418146454967 2315 14-15 -1.149729555130179 2315 16-17 -2.191151707932285 2315 18-19 -1.1926033822243625 2315 20-21 -0.8518759947874841 2315 22-23 -1.0112867487013588 2315 24-25 -2.967448129382742 2315 26-27 -4.238603621544083 2315 28-29 -5.690926604030945 2315 30-31 -8.302232102721252 2315 32-33 -6.973842995960339 2315 34-35 -6.285444244320569 2315 36-37 -4.004343770696689 2315 38-39 -6.217508986383571 2315 40-41 -3.912842195413951 2315 42-43 -3.8619812079727254 2315 44-45 -3.1636487718004886 2315 46-47 -2.282418133081155 2315 48-49 -3.1319263627389624 2315 50-51 -3.0479523512387523 2315 52-53 -2.240973243587895 2315 54-55 -3.626744942025965 2315 56-57 -3.5272184592339286 2315 58-59 -2.3076223266965776 2315 60-61 -2.6149568301350037 2315 62-63 -3.187615006940387 2315 64-65 -2.2651837010733367 2315 66-67 -2.95271029598754 2315 68-69 -2.201986928299082 2315 70-71 -2.598852621137052 2315 72-73 -1.648531825263845 2315 74-75 -2.1646422626437527 2315 76-77 -1.4356786435406619 2315 78-79 -1.2880939176553028 2315 80-81 -1.1475499392709807 2315 82-83 -0.7904270967629827 2315 84-85 -1.0593822875603713 2315 86-87 -1.5567153326786425 2315 88-89 -1.638221471886638 2315 90-91 -1.5077638236918425 2315 92-93 -0.5504495128613911 2315 94-95 -0.16515297666708761 2315 96-97 1.2951770971182697 2315 98-99 1.2799572530914851 2315 100-101 1.009648873790324 2316 1 -3.558660549899667 2316 2 -2.3254361058907875 2316 3 -2.393018390756943 2316 4 -3.1345781925669165 2316 5 -2.4951471429559113 2316 6 -6.206431619304851 2316 7 -3.6525159884613565 2316 8 -4.744822999583729 2316 9 -6.680899092052503 2316 10-11 -5.997964185849618 2316 12-13 -3.826623190636653 2316 14-15 -4.919288930478743 2316 16-17 -4.5795255615365775 2316 18-19 -2.642328238930098 2316 20-21 -3.2698851867626715 2316 22-23 -3.839779998929849 2316 24-25 -2.706495773996327 2316 26-27 -3.668424809617072 2316 28-29 -3.6954467409741447 2316 30-31 -3.552839011976822 2316 32-33 -3.2792450814058363 2316 34-35 -5.2029369443457085 2316 36-37 -5.538874441412052 2316 38-39 -5.270741784829347 2316 40-41 -4.689897363080306 2316 42-43 -3.8039646161370406 2316 44-45 -3.3411290984930915 2316 46-47 -3.567696177220615 2316 48-49 -3.9209708234980596 2316 50-51 -5.066017916166913 2316 52-53 -2.9408140092445514 2316 54-55 -3.3060635805182237 2316 56-57 -2.3668848537122535 2316 58-59 -2.768754018409563 2316 60-61 -3.102689313941049 2316 62-63 -2.18101558411286 2316 64-65 -2.391857409676984 2316 66-67 -3.2009031984475556 2316 68-69 -2.7172642274582124 2316 70-71 -2.3867708714071583 2316 72-73 -2.4245443326095995 2316 74-75 -3.411847025690065 2316 76-77 -3.589449659061227 2316 78-79 -3.564776283013636 2316 80-81 -3.5997599712843247 2316 82-83 -2.591839583632183 2316 84-85 -2.548735727871218 2316 86-87 -2.391194125294433 2316 88-89 -2.2895191735182685 2316 90-91 -1.939588655093221 2316 92-93 -1.4981092341471864 2316 94-95 -1.3526202016944318 2316 96-97 0.4187435302190359 2316 98-99 0.7868882754042712 2316 100-101 0.567801039560516 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18873.0 3 6366.0 4 6295.0 5 7101.0 6 8107.0 7 9849.0 8 11790.0 9 14414.0 10 16799.0 11 19560.0 12 22281.0 13 24655.0 14 27155.0 15 29189.0 16 32042.0 17 34727.0 18 33558.0 19 33969.0 20 37026.0 21 40594.0 22 45053.0 23 50332.0 24 56501.0 25 63294.0 26 71105.0 27 79553.0 28 89949.0 29 101982.0 30 114514.0 31 129844.0 32 148057.0 33 169376.0 34 192251.0 35 210127.0 36 204214.0 37 144560.0 38 32455.0 39 271.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.95710580681853 18.311101099420654 13.143841154443079 23.587951939317737 2 16.102870014302965 19.932253616423573 39.40938171789755 24.555494651375913 3 19.982423132254365 24.170034781126454 28.74905723488974 27.09848485172944 4 13.156854304139332 15.70187894480961 36.39348826650047 34.74777848455059 5 14.268183512016689 37.290666346849875 33.49137137154287 14.949778769590564 6 33.014415754188605 35.10456304707993 17.451903225640645 14.429117973090822 7 29.742526094575727 32.00130735248699 20.16634047108382 18.08982608185346 8 27.224319486250508 34.036160706062866 19.589397919832017 19.150121887854606 9 27.280886072966936 16.351610408915562 19.530831989273224 36.83667152884428 10-11 25.71115191059829 25.894728184601785 26.984619945066335 21.40949995973359 12-13 26.503630688619644 23.26277284245341 27.351615963249976 22.881980505676975 14-15 23.560587727620245 24.617936357347986 25.4038218142803 26.41765410075147 16-17 23.008796680497927 27.838136795609692 26.066443581849818 23.086622942042563 18-19 22.967158897642516 26.582443333461853 28.091480420002828 22.358917348892803 20-21 23.785936173558962 25.78317471214422 28.002050097983883 22.428839016312935 22-23 23.289620228967145 25.64718332180018 28.087789383315414 22.97540706591726 24-25 23.041199956530768 25.947379510602488 28.2129237794481 22.798496753418647 26-27 22.86934786788494 26.09456589794819 28.120439063150748 22.915647171016122 28-29 22.79856896961954 26.422745580546902 28.094367407832944 22.684318042000612 30-31 22.897234424143278 26.141734342582783 27.870666710394236 23.0903645228797 32-33 22.67666072690583 26.205556023562693 27.914989611313285 23.202793638218193 34-35 23.284851975855325 26.16529691402788 27.856594270353856 22.69325683976294 36-37 23.399855157548306 25.84480002742579 27.879043696996447 22.876301118029456 38-39 22.945486557110904 26.179250236045053 27.85494511711392 23.02031808973012 40-41 23.09926314848943 26.03608427563719 28.025341229594307 22.83931134627907 42-43 22.942051971906015 26.003759235776336 27.769125840546177 23.285062951771472 44-45 22.8900200676493 26.111515273591806 27.895468225507663 23.102996433251228 46-47 23.208380950094774 26.326353637720533 27.464997929581774 23.000267482602915 48-49 22.864861295943147 26.407384001701217 27.485585959472626 23.242168742883013 50-51 22.825284341858588 26.61608746288676 27.459850809501674 23.098777385752978 52-53 23.121744157819297 26.411640528431818 27.25624019440911 23.210375119339776 54-55 22.652784913596786 26.488586644663087 27.466396368037287 23.392232073702836 56-57 22.64622059591143 26.69783524171187 27.251559768529386 23.404384393847312 58-59 22.79513612763635 26.2281753576547 27.40761810610955 23.5690704085994 60-61 22.553555895439914 26.4970073675185 27.54760577614822 23.40183096089337 62-63 22.565643410095255 26.2963846277305 27.677597788790397 23.460374173383844 64-65 22.393872939996765 26.537373330490784 27.405352071332384 23.66340165818007 66-67 22.407405423587914 26.55670513927484 27.24730523911651 23.788584198020736 68-69 22.66079894718731 26.261694317926892 27.36815897265914 23.70934776222666 70-71 22.656735798479943 26.215284994632448 27.128733450041032 23.999245756846577 72-73 22.644513555323154 26.39825742842512 27.036245771698177 23.920983244553554 74-75 22.55301013715504 26.79283498412874 26.917365475877336 23.736789402838884 76-77 22.703939693273277 26.38670157600167 27.03763874413017 23.871719986594883 78-79 22.782990825754943 26.620317716628534 26.86049500575735 23.736196451859172 80-81 22.91534767128216 26.64493240990614 26.65016372515956 23.789556193652142 82-83 22.95900658243545 26.597236976466128 26.75168398478427 23.692072456314158 84-85 22.852504164962525 26.697109691779914 26.567766491150902 23.88261965210666 86-87 22.893291540672827 26.50586037467646 26.715009435070343 23.885838649580375 88-89 22.928145532550072 26.50318575368163 26.378546329213552 24.190122384554744 90-91 22.914260629646492 26.54318606028961 26.461027130237657 24.081526179826245 92-93 23.20644624697752 26.395293502306515 26.377244353746164 24.0210158969698 94-95 23.042267337565896 26.25909366864575 26.505535947645164 24.193103046143186 96-97 23.275150785942294 25.922484804272276 26.500140325921596 24.30222408386383 98-99 23.256008996898576 26.070178125464523 26.130494925945687 24.54331795169122 100-101 23.321247006842913 26.026726356043355 26.229495184562108 24.42253145255162 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2777.0 1 1595.5 2 394.5 3 382.0 4 330.5 5 320.5 6 463.5 7 754.5 8 1184.0 9 1409.0 10 1289.0 11 1205.0 12 1235.0 13 1274.0 14 1376.5 15 1505.5 16 1698.0 17 1936.0 18 2174.5 19 2349.0 20 2719.0 21 3318.5 22 3892.0 23 4798.0 24 5919.0 25 7259.5 26 9274.5 27 11661.0 28 14123.0 29 16975.5 30 20611.5 31 25436.5 32 31543.0 33 36252.0 34 39455.5 35 43508.5 36 49414.0 37 55144.5 38 58597.0 39 61715.5 40 64864.5 41 67815.5 42 70570.5 43 73621.0 44 76795.5 45 80749.5 46 83936.0 47 87425.0 48 92167.0 49 93368.0 50 91457.5 51 89645.0 52 88913.0 53 88736.5 54 87967.0 55 83982.0 56 77558.0 57 71046.0 58 65142.5 59 57853.5 60 48467.5 61 39976.0 62 32440.5 63 24749.0 64 18043.5 65 13445.5 66 10177.5 67 7729.0 68 5926.0 69 4540.0 70 3413.5 71 2503.0 72 1870.0 73 1409.0 74 1033.0 75 763.0 76 550.5 77 388.0 78 282.5 79 188.0 80 127.0 81 81.0 82 52.5 83 41.0 84 25.5 85 17.5 86 16.5 87 10.5 88 6.5 89 6.0 90 3.5 91 1.5 92 1.0 93 1.5 94 1.0 95 1.0 96 1.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.11720481070139807 2 0.11155844755811906 3 0.12409166271706418 4 0.1382931215319781 5 0.13337394152078802 6 0.13345949247750438 7 0.1411163031036176 8 0.13966193683943967 9 0.14727597198719472 10-11 0.14269899580287007 12-13 0.14173654753981113 14-15 0.1297380258603432 16-17 0.13262537064951996 18-19 0.15048413286405782 20-21 0.18141080371701798 22-23 0.1992695659315558 24-25 0.21830465380094344 26-27 0.21984457102183774 28-29 0.22256081389758184 30-31 0.22087118250243393 32-33 0.22253942615840272 34-35 0.19334516217894865 36-37 0.18042696771477995 38-39 0.19450010009461938 40-41 0.1830148841554495 42-43 0.18543169868268636 44-45 0.18951675686589203 46-47 0.2107120063923675 48-49 0.2284210544326517 50-51 0.24536014386248883 52-53 0.2656357206042635 54-55 0.25862054215352287 56-57 0.22715917782108558 58-59 0.22076424380653847 60-61 0.19854238279946684 62-63 0.18840459442857949 64-65 0.17989227423530277 66-67 0.17508003292000815 68-69 0.18091888571589895 70-71 0.1849183929423883 72-73 0.22001567293527044 74-75 0.232163908788992 76-77 0.2499157323076344 78-79 0.23751084358376381 80-81 0.2429005538568938 82-83 0.23939296463152349 84-85 0.22388685372668524 86-87 0.21396294274758873 88-89 0.21640114501400468 90-91 0.21875379632370429 92-93 0.22534121999086315 94-95 0.23081648122070952 96-97 8.70356935701612 98-99 0.28926917239715494 100-101 0.3017809998169209 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2337788.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.00213173300337 #Duplication Level Percentage of deduplicated Percentage of total 1 77.19077071041156 42.456569391861144 2 11.982632812895027 13.18140696966122 3 4.384747178336306 7.235113257563048 4 2.071771446195945 4.558073840173771 5 1.1391874291699644 3.1328868523893916 6 0.7258483120198267 2.3953922685535582 7 0.4871353310375655 1.8755437154669867 8 0.32196389031348577 1.4166960246634075 9 0.24092709427165362 1.1926353399461294 >10 1.318393851826812 13.137788335439145 >50 0.09253296492824964 3.4912203811006806 >100 0.04100537280806053 4.02183673641486 >500 0.002055737190402192 0.7807478511477718 >1k 9.488017801853522E-4 0.8915998687811609 >5k 7.906681501544602E-5 0.232489166837581 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5346 0.22867770730280074 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3826 0.16365898019837555 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2607 0.11151567207976086 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 4.277547835817448E-5 0.0 0.0 0.0 4 0.0 4.277547835817448E-5 0.0 4.277547835817448E-5 0.0 5 0.0 4.277547835817448E-5 0.0 4.277547835817448E-5 0.0 6 0.0 4.277547835817448E-5 0.0 4.277547835817448E-5 4.277547835817448E-5 7 4.277547835817448E-5 4.277547835817448E-5 0.0 4.277547835817448E-5 8.555095671634896E-5 8 4.277547835817448E-5 4.277547835817448E-5 0.0 4.277547835817448E-5 8.555095671634896E-5 9 4.277547835817448E-5 4.277547835817448E-5 0.0 8.555095671634896E-5 8.555095671634896E-5 10-11 4.277547835817448E-5 4.277547835817448E-5 0.0 1.2832643507452345E-4 8.555095671634896E-5 12-13 8.555095671634896E-5 4.277547835817448E-5 0.0 1.2832643507452345E-4 1.069386958954362E-4 14-15 1.2832643507452345E-4 4.277547835817448E-5 0.0 1.7110191343269792E-4 1.7110191343269792E-4 16-17 1.2832643507452345E-4 4.277547835817448E-5 0.0 2.9942834850722137E-4 2.138773917908724E-4 18-19 1.4971417425361068E-4 4.277547835817448E-5 0.0 3.4220382686539583E-4 2.138773917908724E-4 20-21 1.7110191343269792E-4 4.277547835817448E-5 0.0 3.849793052235703E-4 2.7804060932813413E-4 22-23 1.7110191343269792E-4 4.277547835817448E-5 0.0 5.34693479477181E-4 3.849793052235703E-4 24-25 3.4220382686539583E-4 4.277547835817448E-5 0.0 0.0011121624373125365 4.277547835817448E-4 26-27 3.4220382686539583E-4 4.277547835817448E-5 0.0 0.0022029371354459855 4.277547835817448E-4 28-29 4.705302619399193E-4 4.277547835817448E-5 0.0 0.007335994538426923 4.277547835817448E-4 30-31 5.560812186562683E-4 4.277547835817448E-5 0.0 0.027611571280201627 4.277547835817448E-4 32-33 5.560812186562683E-4 4.277547835817448E-5 0.0 0.07338133312344831 5.133057402980938E-4 34-35 5.774689578353556E-4 4.277547835817448E-5 0.0 0.14351172989167538 5.133057402980938E-4 36-37 5.988566970144427E-4 4.277547835817448E-5 0.0 0.26443800721023464 5.133057402980938E-4 38-39 6.630199145517045E-4 4.277547835817448E-5 0.0 0.44490775040337277 5.133057402980938E-4 40-41 6.844076537307917E-4 4.277547835817448E-5 0.0 0.6501231078267149 5.133057402980938E-4 42-43 7.913463496262279E-4 4.277547835817448E-5 0.0 0.8576483410813983 5.560812186562683E-4 44-45 9.624482630589259E-4 4.277547835817448E-5 0.0 1.0988378758039652 6.202444361935299E-4 46-47 0.001261876611566147 4.277547835817448E-5 0.0 1.3835514597559744 6.844076537307917E-4 48-49 0.001326039829103409 4.277547835817448E-5 0.0 1.6839422565262547 6.844076537307917E-4 50-51 0.001326039829103409 4.277547835817448E-5 0.0 2.0040311610804746 6.844076537307917E-4 52-53 0.001326039829103409 4.277547835817448E-5 0.0 2.3108168918653016 7.057953929098788E-4 54-55 0.001326039829103409 4.277547835817448E-5 0.0 2.6268849014538533 7.485708712680534E-4 56-57 0.0013688153074615833 4.277547835817448E-5 0.0 2.998304380037882 7.699586104471406E-4 58-59 0.0013688153074615833 4.277547835817448E-5 0.0 3.3922023724991317 7.699586104471406E-4 60-61 0.0013688153074615833 4.277547835817448E-5 0.0 3.8175617292928186 7.699586104471406E-4 62-63 0.0013688153074615833 4.277547835817448E-5 0.0 4.234173500762259 7.699586104471406E-4 64-65 0.0013902030466406705 4.277547835817448E-5 0.0 4.6410110754268565 7.699586104471406E-4 66-67 0.0014971417425361068 4.277547835817448E-5 0.0 5.090688291667165 7.699586104471406E-4 68-69 0.0015399172208942812 4.277547835817448E-5 4.277547835817448E-5 5.595374773076088 7.699586104471406E-4 70-71 0.0015826926992524558 4.277547835817448E-5 4.277547835817448E-5 6.12720229550327 7.699586104471406E-4 72-73 0.001604080438431543 4.277547835817448E-5 4.277547835817448E-5 6.648528437993522 0.0010266114805961876 74-75 0.0016254681776106301 4.277547835817448E-5 4.277547835817448E-5 7.153172143924085 0.001069386958954362 76-77 0.0016682436559688047 4.277547835817448E-5 4.277547835817448E-5 7.685919339135969 0.001069386958954362 78-79 0.0016682436559688047 4.277547835817448E-5 4.277547835817448E-5 8.277461429351163 0.001069386958954362 80-81 0.0018821210477596771 4.277547835817448E-5 4.277547835817448E-5 8.900251006507006 0.001069386958954362 82-83 0.0020104474828342004 6.416321753726173E-5 4.277547835817448E-5 9.514250222860241 0.001069386958954362 84-85 0.0020746107003714624 8.555095671634896E-5 4.277547835817448E-5 10.117940548929159 0.001069386958954362 86-87 0.002138773917908724 8.555095671634896E-5 4.277547835817448E-5 10.646902114306345 0.0010907746981334491 88-89 0.002138773917908724 8.555095671634896E-5 4.277547835817448E-5 11.181424491870093 0.0011549379156707109 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9310 0.0 30.824934 1 TACATGG 9845 0.0 28.24189 2 GTACATG 10320 0.0 27.625805 1 GGTATCA 6195 0.0 27.339035 1 ACATGGG 10605 0.0 25.330715 3 ATCAACG 11350 0.0 25.243145 3 CAACGCA 11380 0.0 25.09499 5 TCAACGC 11430 0.0 25.067 4 TATCAAC 11610 0.0 24.677835 2 AACGCAG 11720 0.0 24.327358 6 CATGGGA 5000 0.0 21.735683 4 GTATAGG 660 0.0 21.384512 1 CATGGGG 5610 0.0 21.13338 4 ACGCAGA 13310 0.0 21.103096 7 CGCAGAG 13640 0.0 20.523552 8 AGAGTAC 10420 0.0 19.910778 10-11 GAGTACT 5750 0.0 19.881592 12-13 GAGTACA 7815 0.0 19.143448 1 AGTACTT 6200 0.0 19.121483 12-13 GCAGAGT 13965 0.0 18.934128 9 >>END_MODULE