FastQCFastQC Report
Fri 27 May 2016
SRR939774_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939774_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2337788
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62200.26606347538784525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46220.19770826097148245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33600.14372560728346626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA87450.032.0733221
GGTATCA57700.031.0085741
TACATGG106350.028.1107772
GTACATG110700.027.5663721
ATCAACG108300.025.5381323
ACATGGG112450.025.5237793
TCAACGC109800.025.1892494
CAACGCA110350.025.0207145
TATCAAC112200.024.8264732
AACGCAG113950.024.230246
CATGGGG55600.021.0751764
CATGGGA52600.020.9243184
ACGCAGA131850.020.8327927
CGCAGAG135050.020.3040338
GTATAGG7250.019.6381031
GAGTACT63400.019.56732712-13
GTAAGGT9700.019.5630844
AGTACTT66600.019.2682412-13
GGACCGA5700.019.1426496
AGAGTAC116100.019.12329510-11
Produced by FastQC (version 0.11.5)