##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939774_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2337788 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.397534763631263 31.0 31.0 34.0 30.0 34.0 2 31.626086283272905 31.0 31.0 34.0 30.0 34.0 3 31.67421810703109 31.0 31.0 34.0 30.0 34.0 4 35.48304380037882 37.0 35.0 37.0 33.0 37.0 5 35.220432733849265 37.0 35.0 37.0 32.0 37.0 6 35.23975655619757 37.0 35.0 37.0 32.0 37.0 7 35.14160565457603 37.0 35.0 37.0 32.0 37.0 8 35.1044098096149 37.0 35.0 37.0 32.0 37.0 9 36.68998771488261 39.0 35.0 39.0 32.0 39.0 10-11 36.55345095449202 38.0 35.0 39.0 32.0 39.0 12-13 36.47207573997299 38.0 35.0 39.0 32.0 39.0 14-15 37.51500285740195 39.0 36.0 41.0 32.0 41.0 16-17 37.43106410846492 39.0 36.0 41.0 32.0 41.0 18-19 37.401820224930574 39.0 36.0 41.0 32.0 41.0 20-21 37.3051658234194 39.0 36.0 40.5 31.0 41.0 22-23 37.17530631520052 39.0 36.0 40.0 31.0 41.0 24-25 37.01793960786864 39.0 36.0 40.0 31.0 41.0 26-27 36.71261701232105 39.0 35.5 40.0 30.0 41.0 28-29 36.489997809895506 38.0 35.0 40.0 30.0 41.0 30-31 36.2297770370966 38.0 35.0 40.0 30.0 41.0 32-33 36.21815900329713 38.0 35.0 40.0 30.0 41.0 34-35 36.073688674935454 38.0 35.0 40.0 30.0 41.0 36-37 36.13477163027614 38.0 35.0 40.0 30.0 41.0 38-39 36.0422069922508 38.0 35.0 40.0 29.0 41.0 40-41 35.958586492872755 38.0 35.0 40.0 29.0 41.0 42-43 35.7317598943959 38.0 34.5 40.0 28.5 41.0 44-45 35.49468749946531 38.0 34.0 40.0 27.0 41.0 46-47 35.217109720813006 38.0 34.0 40.0 27.0 41.0 48-49 34.91430788420507 38.0 33.5 40.0 26.0 41.0 50-51 34.681208903459165 38.0 33.0 40.0 26.0 41.0 52-53 34.430349757976344 38.0 33.0 40.0 24.5 41.0 54-55 34.151832629819296 37.0 33.0 40.0 24.0 41.0 56-57 33.77331712713043 37.0 32.5 40.0 23.5 41.0 58-59 33.39165377698919 36.0 32.0 39.0 23.0 41.0 60-61 33.054798382060305 36.0 31.0 39.0 22.0 40.5 62-63 32.75397127541078 35.5 31.0 39.0 22.0 40.0 64-65 32.30057173704374 35.0 31.0 38.5 20.5 40.0 66-67 31.341833605100206 34.5 29.5 38.0 15.5 40.0 68-69 29.341476215978524 34.0 26.0 37.0 7.5 39.0 70-71 28.6810934096676 33.0 25.0 36.0 8.5 39.0 72-73 29.288814896816994 33.5 26.5 36.0 9.0 39.0 74-75 29.435481318237585 33.5 27.5 36.0 8.0 38.5 76-77 29.005428849835827 32.5 27.5 35.0 8.0 37.0 78-79 29.555188922177717 34.0 29.0 35.0 8.0 37.0 80-81 29.384179403778273 34.0 29.0 35.0 7.0 37.0 82-83 28.95565701423739 33.5 28.5 35.0 6.0 36.0 84-85 28.611413438686483 33.0 28.0 35.0 2.0 36.0 86-87 28.25006095505666 33.0 27.0 35.0 2.0 35.5 88-89 27.800639750054323 33.0 26.5 35.0 2.0 35.0 90-91 27.31716648387279 33.0 26.0 35.0 2.0 35.0 92-93 26.8310345078339 32.0 25.0 34.0 2.0 35.0 94-95 25.999505943224964 32.0 24.0 34.0 2.0 35.0 96-97 25.486908778725873 32.0 23.0 34.0 2.0 35.0 98-99 24.82064519965027 31.5 19.0 34.0 2.0 35.0 100-101 23.327772449854308 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.27581031858058225 1101 2 0.30223984166665474 1101 3 0.31083382684436955 1101 4 0.31214208819493194 1101 5 0.29755862740499595 1101 6 0.26940816527837086 1101 7 0.21128252603610065 1101 8 0.18288925665699196 1101 9 0.3641121412725852 1101 10-11 0.3725412980966567 1101 12-13 0.40805918991586054 1101 14-15 0.4485602042008523 1101 16-17 0.4397679492344082 1101 18-19 0.37508181603148927 1101 20-21 0.40297429774187066 1101 22-23 0.34542880151344235 1101 24-25 0.40680414094894246 1101 26-27 0.4080797689132041 1101 28-29 0.4199008598881875 1101 30-31 0.2397744925536358 1101 32-33 0.16632439796834575 1101 34-35 0.1386571893799129 1101 36-37 0.4579535529225325 1101 38-39 0.4256998583360243 1101 40-41 0.4657712247757715 1101 42-43 0.28085206381591377 1101 44-45 0.21588315526243207 1101 46-47 0.11853663552074778 1101 48-49 0.2848023448520749 1101 50-51 0.3837503027351019 1101 52-53 0.06804701294230853 1101 54-55 -0.02957836997657637 1101 56-57 -0.06582495556722279 1101 58-59 -0.04161824571828987 1101 60-61 -0.04098444362000464 1101 62-63 -0.10831666585722388 1101 64-65 -0.04238990919129293 1101 66-67 -0.11895242617544 1101 68-69 -8.757277076455711 1101 70-71 -6.7224117249722966 1101 72-73 -1.7753838277758582 1101 74-75 -0.6245447297375968 1101 76-77 -0.40592095405970596 1101 78-79 -0.587715208113579 1101 80-81 -0.5149335487445406 1101 82-83 -0.2843282995158454 1101 84-85 -0.18328954097566097 1101 86-87 -0.24580084661681667 1101 88-89 -0.3182484896601885 1101 90-91 -0.48101650049044054 1101 92-93 -0.6582472999388465 1101 94-95 -0.3592844235852617 1101 96-97 -0.9011419410001906 1101 98-99 -0.7565320950425409 1101 100-101 -0.7654022678448698 1102 1 0.17279915419211989 1102 2 0.11493345485944317 1102 3 0.13606756844807322 1102 4 0.1302530368612409 1102 5 0.3168937481991847 1102 6 0.20440972440771787 1102 7 0.3111952403524896 1102 8 0.08965204407353866 1102 9 0.36490001277987716 1102 10-11 0.4510872310694296 1102 12-13 0.30732889117645357 1102 14-15 0.39322804575241577 1102 16-17 0.41339757678141353 1102 18-19 0.3563748198858576 1102 20-21 0.20981702248653278 1102 22-23 0.17817000649038306 1102 24-25 0.325892356998267 1102 26-27 0.281011047092548 1102 28-29 0.05474094689271425 1102 30-31 0.015376163122382991 1102 32-33 -0.09460388463789826 1102 34-35 -0.036416741466311464 1102 36-37 0.09698507800662526 1102 38-39 0.10941545525252394 1102 40-41 0.09974867874311144 1102 42-43 0.12092181568431926 1102 44-45 0.10500988947433854 1102 46-47 0.12176320257353268 1102 48-49 0.14767308536183066 1102 50-51 0.14025838976007066 1102 52-53 0.04270061785382495 1102 54-55 -0.10150407362954184 1102 56-57 -0.2883973784030971 1102 58-59 -0.16680355207442688 1102 60-61 -0.11287915638237678 1102 62-63 0.05730408487634264 1102 64-65 -0.11021970338622111 1102 66-67 0.07140097221533637 1102 68-69 -4.395047240343207 1102 70-71 0.09432572020258334 1102 72-73 0.5988698349001211 1102 74-75 0.33700963275741813 1102 76-77 0.015451322575849247 1102 78-79 -0.13662777929678072 1102 80-81 -0.19024997532232746 1102 82-83 -0.07050425365187962 1102 84-85 -0.11058555062692932 1102 86-87 -0.21631860535561032 1102 88-89 -0.22757904808408824 1102 90-91 -0.29094042264643605 1102 92-93 -0.3965570248164134 1102 94-95 -0.09271388192839325 1102 96-97 -0.847798311449317 1102 98-99 -0.6639559149605496 1102 100-101 -0.8709157070286047 1103 1 0.18967552278888178 1103 2 0.21689359311656276 1103 3 0.2452559489013062 1103 4 0.22639647862248324 1103 5 0.25365816750390024 1103 6 0.218693130989287 1103 7 0.22198260336460862 1103 8 0.27604466659980886 1103 9 0.40060788820488114 1103 10-11 0.3155503180596213 1103 12-13 0.3016262978298201 1103 14-15 0.3581628018280867 1103 16-17 0.5112192322770781 1103 18-19 0.35737129935498757 1103 20-21 0.3395401236306341 1103 22-23 0.303526418574549 1103 24-25 0.41093638286506007 1103 26-27 0.3331747761373123 1103 28-29 0.18715669005037228 1103 30-31 0.2835922821793986 1103 32-33 0.3646497525602399 1103 34-35 0.20302871722834936 1103 36-37 0.22883479262054607 1103 38-39 0.45374606720438493 1103 40-41 0.41039931028480936 1103 42-43 0.03471848900058916 1103 44-45 0.3209875996164371 1103 46-47 0.2783274670056599 1103 48-49 0.16559689522752308 1103 50-51 0.27691614185111035 1103 52-53 0.31482605183040846 1103 54-55 0.33619817099734917 1103 56-57 0.4339912620514639 1103 58-59 0.3410955268952094 1103 60-61 0.2731670541110063 1103 62-63 0.12494641893959368 1103 64-65 0.23456187241672666 1103 66-67 0.4307457637270815 1103 68-69 -4.0514980426550125 1103 70-71 0.5701297627874133 1103 72-73 0.9221625184523425 1103 74-75 0.6439765915623354 1103 76-77 0.47308219504186155 1103 78-79 0.2948103306422425 1103 80-81 0.3303318609947823 1103 82-83 0.2320614005309629 1103 84-85 0.18151530630054324 1103 86-87 0.27204575805138376 1103 88-89 0.1408087385199437 1103 90-91 0.18246905637653654 1103 92-93 -0.011620755912471026 1103 94-95 -0.06215282776913966 1103 96-97 -0.40175179326204713 1103 98-99 -0.05977493198114914 1103 100-101 -0.06986544523238791 1104 1 0.21988504918187246 1104 2 0.25814708438587886 1104 3 0.22821905698497602 1104 4 0.20948014852422148 1104 5 0.2290472305376383 1104 6 0.19156295436622628 1104 7 0.09289731592514272 1104 8 0.1695069508443865 1104 9 0.1984119049227857 1104 10-11 0.33768345849404824 1104 12-13 0.17202611095900266 1104 14-15 0.17926790159299344 1104 16-17 0.345366985808532 1104 18-19 0.0975222779150755 1104 20-21 0.3005201656263381 1104 22-23 0.25372057333573395 1104 24-25 0.20952091176975784 1104 26-27 0.2497654637415465 1104 28-29 0.23639677384083768 1104 30-31 0.058611371133928 1104 32-33 0.15731231007827517 1104 34-35 0.15074633130996773 1104 36-37 0.32110351486620914 1104 38-39 0.34141887247695735 1104 40-41 0.2997354918910986 1104 42-43 0.40320815082468897 1104 44-45 0.3704719466737245 1104 46-47 0.2920960033851898 1104 48-49 0.3630105930594212 1104 50-51 0.30633322546073316 1104 52-53 0.05950186612502506 1104 54-55 0.1352731102141007 1104 56-57 0.2943718857579398 1104 58-59 0.3906690394637806 1104 60-61 0.21666437663396465 1104 62-63 0.28658779274150703 1104 64-65 0.27818829655320343 1104 66-67 0.5183207381077004 1104 68-69 -4.142529750413164 1104 70-71 0.9389107823385245 1104 72-73 1.0720516266967621 1104 74-75 0.6918823323142398 1104 76-77 0.5213213645718682 1104 78-79 0.27023455753958814 1104 80-81 0.08523219655851122 1104 82-83 0.306804076400514 1104 84-85 0.2900259659746247 1104 86-87 0.2702166092178153 1104 88-89 0.3651567000205418 1104 90-91 0.12060640140436263 1104 92-93 0.04257366508939597 1104 94-95 0.5396660230757995 1104 96-97 0.06352326350247495 1104 98-99 0.265590646615113 1104 100-101 0.3527200531902608 1105 1 0.30579348858539035 1105 2 0.3175433730085011 1105 3 0.2882639225786292 1105 4 0.1843794104128449 1105 5 0.19413531766880254 1105 6 0.22804433781089273 1105 7 0.2053153670267207 1105 8 0.26439612622645825 1105 9 0.3760967628484053 1105 10-11 0.2990305244568816 1105 12-13 0.29142219645500234 1105 14-15 0.3373153341993813 1105 16-17 0.30987243277598253 1105 18-19 0.19317579657606387 1105 20-21 0.32455932907471663 1105 22-23 0.2878326751123339 1105 24-25 0.3806207377156312 1105 26-27 0.2345243810004547 1105 28-29 0.24430038327403736 1105 30-31 0.4031874458642122 1105 32-33 0.40455280311737596 1105 34-35 0.4046554080232809 1105 36-37 0.3053259715304151 1105 38-39 0.41452049545869585 1105 40-41 0.3455252323460556 1105 42-43 0.28944882921684467 1105 44-45 0.17141673939569557 1105 46-47 0.12497428546713252 1105 48-49 0.2533547466942423 1105 50-51 0.3313206570007097 1105 52-53 0.31742705776229485 1105 54-55 0.32325045778661377 1105 56-57 0.12966824750615302 1105 58-59 0.23404460409232541 1105 60-61 0.39163249527515376 1105 62-63 0.4523503078807849 1105 64-65 0.28308214067383375 1105 66-67 0.8422935045400095 1105 68-69 2.1363681860742183 1105 70-71 2.427282259043711 1105 72-73 1.546525830249955 1105 74-75 1.1203436735085717 1105 76-77 0.7347651575299778 1105 78-79 0.5837895464564902 1105 80-81 0.520318798326219 1105 82-83 0.5617502891045589 1105 84-85 0.48210694748248173 1105 86-87 0.30983986962868926 1105 88-89 0.46310372459632276 1105 90-91 0.2721311682530789 1105 92-93 0.2677645247720619 1105 94-95 0.8456566875029949 1105 96-97 0.19100847017111988 1105 98-99 0.4699413491793294 1105 100-101 0.4486594567089206 1106 1 0.3793049728117026 1106 2 0.37766920927357006 1106 3 0.2951917658798564 1106 4 0.2549821700875654 1106 5 0.3009025764495874 1106 6 0.29008889776245184 1106 7 0.28224760792873127 1106 8 0.37926380169793816 1106 9 0.4262112086065244 1106 10-11 0.3857180496890109 1106 12-13 0.35414369856008676 1106 14-15 0.6014292897331543 1106 16-17 0.4788645711634345 1106 18-19 0.4896788929195708 1106 20-21 0.4616654374142968 1106 22-23 0.5113210899574838 1106 24-25 0.42613563548943034 1106 26-27 0.546646079195277 1106 28-29 0.5835581619809957 1106 30-31 0.651368839687386 1106 32-33 0.6159348392786299 1106 34-35 0.5680475442479747 1106 36-37 0.6323933845876795 1106 38-39 0.778040053968418 1106 40-41 0.7431626033017054 1106 42-43 0.7987859810133671 1106 44-45 0.9373885010313145 1106 46-47 0.8468635236008168 1106 48-49 0.7182062916218612 1106 50-51 0.8672623022801389 1106 52-53 0.6697346266820787 1106 54-55 0.7640607337540857 1106 56-57 0.7376810726402425 1106 58-59 0.7484352119705164 1106 60-61 0.8494628379996456 1106 62-63 0.8164985070272195 1106 64-65 0.771925661782312 1106 66-67 1.2625677057626845 1106 68-69 2.7972782010693145 1106 70-71 3.1547365348093237 1106 72-73 2.0075651599402455 1106 74-75 1.4570763462405658 1106 76-77 1.0509148057219129 1106 78-79 0.8596929470274546 1106 80-81 1.0567253457049581 1106 82-83 1.0845795144059025 1106 84-85 1.0613646901435345 1106 86-87 1.0271404636652548 1106 88-89 1.1986248042433587 1106 90-91 0.911620271575071 1106 92-93 0.832689062663519 1106 94-95 1.3887493002545952 1106 96-97 1.1147418169615975 1106 98-99 1.4276722953123233 1106 100-101 1.310590111590976 1107 1 0.0857530570122691 1107 2 0.07604610142115575 1107 3 0.031246046863774524 1107 4 -0.023737082514138308 1107 5 0.0292966161846806 1107 6 0.05712118253066478 1107 7 0.13119805731378875 1107 8 0.1514876664093876 1107 9 0.13009898060273883 1107 10-11 -0.005962413887139917 1107 12-13 0.014393816819307403 1107 14-15 0.021713703167399956 1107 16-17 0.04799152244063265 1107 18-19 0.09065896624028369 1107 20-21 0.12273006608033654 1107 22-23 0.08753029052512318 1107 24-25 0.13100348080637048 1107 26-27 0.18405442829650553 1107 28-29 0.1775594628324697 1107 30-31 0.08089077677347944 1107 32-33 0.1800993378595237 1107 34-35 0.21532758209092862 1107 36-37 -0.005440697720736409 1107 38-39 0.12529939644712584 1107 40-41 0.060632447199523654 1107 42-43 0.06838449094714605 1107 44-45 0.02307617842014764 1107 46-47 -0.1234356722364538 1107 48-49 0.0723084084619714 1107 50-51 0.07863736140975419 1107 52-53 0.0867624767293762 1107 54-55 0.07507953697073333 1107 56-57 -0.11032921591226597 1107 58-59 -0.07813172275039193 1107 60-61 0.18299080630617226 1107 62-63 0.13261428280772947 1107 64-65 0.13325046074163538 1107 66-67 0.7048601608241825 1107 68-69 1.9857893174363888 1107 70-71 2.325720155906769 1107 72-73 1.3396296703470618 1107 74-75 0.5563446172907085 1107 76-77 0.3172937082763134 1107 78-79 0.4640759295250909 1107 80-81 0.34393856708623005 1107 82-83 0.5658280361656018 1107 84-85 0.38589756620786986 1107 86-87 0.28762414389231594 1107 88-89 0.5374020038649334 1107 90-91 0.5711002266365668 1107 92-93 0.3081576648394879 1107 94-95 0.591133701862919 1107 96-97 0.2955365189483494 1107 98-99 0.45897255548262095 1107 100-101 0.565305698156731 1108 1 -0.1653577419447103 1108 2 -0.1942278547414915 1108 3 -0.08338922376476532 1108 4 0.00698411172088953 1108 5 -0.034085067701980165 1108 6 -0.07074821096901474 1108 7 -0.08841828404585073 1108 8 -0.09769474737242945 1108 9 -0.19805994342925004 1108 10-11 -0.14307107716436462 1108 12-13 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-0.47124344195997026 2316 66-67 0.08947720911348966 2316 68-69 1.2025569649204186 2316 70-71 1.4271513753972087 2316 72-73 0.3797208106933532 2316 74-75 -0.08965257436855367 2316 76-77 -0.3999075517858799 2316 78-79 -0.5389473430992737 2316 80-81 -0.6163614186106123 2316 82-83 -0.3363027885268792 2316 84-85 -0.5305834221250585 2316 86-87 -0.6016150933648028 2316 88-89 -0.5757630930428164 2316 90-91 -0.6093518147164794 2316 92-93 -0.6010376517217217 2316 94-95 -0.2565911922475621 2316 96-97 -0.3279137798800207 2316 98-99 -0.4448683041833945 2316 100-101 -0.35782625925368805 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 45.0 5 123.0 6 226.0 7 621.0 8 1396.0 9 2412.0 10 3790.0 11 5704.0 12 7812.0 13 10109.0 14 12272.0 15 15102.0 16 18316.0 17 21389.0 18 20253.0 19 18020.0 20 18215.0 21 19237.0 22 21017.0 23 23447.0 24 28324.0 25 34542.0 26 42979.0 27 53273.0 28 65195.0 29 78929.0 30 93591.0 31 111380.0 32 133411.0 33 161664.0 34 202497.0 35 254743.0 36 319513.0 37 340991.0 38 183650.0 39 13588.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.946783840907734 19.171337377955382 14.042083465254187 23.8397953158827 2 16.251952130549935 20.776017354173174 38.452971303146086 24.519059212130806 3 19.78524143335495 24.414788680581815 29.31454862459727 26.48542126146597 4 13.499983745318222 16.431130624333772 36.077993385200024 33.99089224514798 5 13.946131984593984 37.66616134568233 33.71875465183327 14.668952017890415 6 33.0051454644927 35.71467374914823 16.935503533897656 14.34467725246141 7 29.424267726585985 32.249031990924756 20.123980446473333 18.202719836015927 8 26.999197532026 34.685480462728016 19.312700723932196 19.00262128131379 9 26.702677916047136 16.307552267357007 19.505532580370847 37.48423723622501 10-11 25.518417409961895 26.188602217138595 26.993251740534213 21.299728632365294 12-13 26.244638093787803 23.329147039851346 27.51725135042185 22.908963515939 14-15 23.095165173232132 24.825754088907974 25.4611410444403 26.61793969341959 16-17 22.765307986241666 28.109131600323895 26.11005811916675 23.01550229426769 18-19 22.664651371296284 26.789640463549304 28.188505544557508 22.357202620596905 20-21 23.46527999972624 25.940504442661183 28.121326655795993 22.47288890181659 22-23 23.097496436802654 25.819278736994118 28.110055317248612 22.97316950895462 24-25 22.89724613314609 26.10752782922129 28.236593990338292 22.75863204729433 26-27 22.68960008041809 26.237727419039196 28.15537801313852 22.917294487404195 28-29 22.671830810897404 26.501799886345477 28.149640696445122 22.676728606311997 30-31 22.75496513672934 26.265353538010817 27.87438314756913 23.105298177690713 32-33 22.585308481088436 26.197050009699552 27.976591887066824 23.241049622145184 34-35 23.162851043433232 26.28283073279772 27.87399449414091 22.680323729628142 36-37 23.248770872893814 25.955922922816406 27.939312176981456 22.85599402730832 38-39 22.793572512615604 26.25938208962062 27.937159508208197 23.00988588955558 40-41 22.96152083061685 26.12611397408401 28.04061960114787 22.87174559415127 42-43 22.80697697572318 26.07831487173228 27.816413856745687 23.29829429579885 44-45 22.77077962686345 26.192864362425833 27.948416953963495 23.087939056747217 46-47 23.06011598114707 26.37841075685748 27.517863014824762 23.04361024717069 48-49 22.76758284739774 26.483293506524646 27.494413471209306 23.254710174868308 50-51 22.71960273118032 26.62564701117126 27.481527881905183 23.17322237574324 52-53 23.063114201278548 26.390987373135793 27.330965622386707 23.214932803198955 54-55 22.58224751116783 26.466659803923875 27.502320455988595 23.448772228919697 56-57 22.565462859235918 26.66745543596121 27.289007066402966 23.478074638399914 58-59 22.64364246040455 26.240634571048062 27.386475792240528 23.72924717630686 60-61 22.418193131437928 26.54926807335929 27.56350790042709 23.469030894775692 62-63 22.448098310460075 26.384276759960596 27.57812362981807 23.589501299761263 64-65 22.241111063104746 26.48147522322603 27.40372501821271 23.87368869545651 66-67 21.94233407179416 26.54932701946113 27.56078483796601 23.947554070778697 68-69 22.05664786796614 26.23032027821475 27.87270931786989 23.840322535949213 70-71 22.085873495929512 26.18758910166621 27.591330952850374 24.135206449553905 72-73 22.060693232232946 26.279449962359507 27.52204695707741 24.137809848330136 74-75 22.279387696158658 26.67394071649391 26.899350684081597 24.147320903265836 76-77 22.24134932050662 26.38050473716607 27.12404393926654 24.254102003060762 78-79 22.334865344437276 26.523933253139454 26.98777157406441 24.15342982835886 80-81 22.410418213736957 26.484246404237915 26.8150770483781 24.290258333647024 82-83 22.43799370943546 26.510670133763252 26.84431900767692 24.20701714912436 84-85 22.267129498926334 26.561374466374083 26.723536859755836 24.44795917494375 86-87 22.36277489241283 26.347900796297985 26.831505689966296 24.45781862132289 88-89 22.234522572342563 26.362920261863426 26.639400098426563 24.763157067367445 90-91 22.1732582019368 26.33429450532152 26.770522005790987 24.72192528695069 92-93 22.249285576636595 26.226593424503186 26.773813595229036 24.75030740363118 94-95 21.881334867151615 25.988330251568748 27.131844011194534 24.998490870085107 96-97 22.101699773677254 25.591220046462475 27.20619756392869 25.10088261593158 98-99 21.969383869629024 25.88273799591842 26.83296753132848 25.31491060312408 100-101 21.760999374556693 25.685998369227164 27.14062463904392 25.412377617172226 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 327.0 1 227.5 2 176.0 3 255.5 4 274.5 5 276.5 6 432.5 7 835.0 8 1301.5 9 1499.5 10 1403.5 11 1269.5 12 1295.0 13 1410.0 14 1577.0 15 1731.0 16 1878.0 17 2040.5 18 2277.5 19 2576.0 20 2929.0 21 3369.5 22 3906.0 23 4804.5 24 5945.5 25 7344.5 26 9370.0 27 11878.5 28 14223.5 29 17088.0 30 20581.0 31 25384.5 32 31936.5 33 36631.0 34 39526.0 35 43606.5 36 49418.0 37 54623.5 38 58337.5 39 61897.0 40 64854.0 41 67410.0 42 70003.0 43 73189.5 44 77183.0 45 81345.0 46 84525.5 47 88210.5 48 93012.5 49 93746.5 50 91456.5 51 90345.5 52 89533.5 53 89794.5 54 89644.5 55 85204.5 56 78472.0 57 71719.0 58 65654.0 59 58140.5 60 48199.5 61 39467.0 62 31761.0 63 23934.5 64 17137.0 65 12402.5 66 9127.0 67 6900.0 68 5282.5 69 4008.0 70 3010.0 71 2065.0 72 1472.0 73 1215.5 74 929.5 75 625.5 76 415.0 77 258.0 78 170.5 79 100.0 80 55.0 81 38.0 82 29.5 83 14.0 84 6.5 85 8.0 86 6.0 87 2.0 88 1.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022585452573116126 2 0.025579736058188342 3 0.0 4 0.0 5 0.0 6 4.277547835817448E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.1387739179087238E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.0693869589543619E-4 26-27 2.3526513096995964E-4 28-29 6.416321753726171E-5 30-31 5.34693479477181E-4 32-33 0.0022029371354459855 34-35 0.004127833661563838 36-37 0.1066178798077499 38-39 0.09784890674432413 40-41 0.10743061389655521 42-43 0.1084572253771514 44-45 0.10135649596969443 46-47 0.09564596960887814 48-49 0.09662980561111614 50-51 0.095859847000669 52-53 0.07599063730329696 54-55 0.06422738075479897 56-57 0.03481923938355403 58-59 0.014329785249988451 60-61 0.009603094891410171 62-63 0.0021815493962668983 64-65 0.005774689578353555 66-67 0.1659474682905379 68-69 0.4377428577783785 70-71 0.4144516098123525 72-73 0.19375152922335132 74-75 0.030498916069378403 76-77 0.033771240163778755 78-79 0.005133057402980938 80-81 0.0015399172208942812 82-83 4.277547835817448E-5 84-85 3.4220382686539583E-4 86-87 0.0013902030466406705 88-89 1.9248965261178515E-4 90-91 7.271831320889661E-4 92-93 0.0027162428757440795 94-95 0.08576483410813983 96-97 0.017880149953716933 98-99 0.01959116908804391 100-101 0.010522767676110921 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2337788.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.37441245852882 #Duplication Level Percentage of deduplicated Percentage of total 1 72.29230666821933 34.97097860346896 2 14.058207668571857 13.601150723742958 3 5.400153111496454 7.8368770186421175 4 2.5677096996265765 4.968457923340046 5 1.4560320274503242 3.5217346924354973 6 0.9424002287901042 2.7352834421102656 7 0.6189071016301745 2.095748718543947 8 0.4290834817064804 1.6605329058568712 9 0.31011462108570653 1.3501451330838312 >10 1.731032127652769 15.282517268544169 >50 0.12716575991453286 4.270653999800001 >100 0.0625448360758468 5.371954532041342 >500 0.003013279683983799 1.012813924814987 >1k 0.001240762222816804 1.0544857473225782 >5k 8.862587305834314E-5 0.2666653662524978 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6220 0.26606347538784525 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4622 0.19770826097148245 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3360 0.14372560728346626 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.277547835817448E-5 0.0 0.0 0.0 0.0 3 4.277547835817448E-5 0.0 0.0 0.0 0.0 4 4.277547835817448E-5 0.0 0.0 0.0 8.555095671634896E-5 5 4.277547835817448E-5 0.0 0.0 4.277547835817448E-5 8.555095671634896E-5 6 4.277547835817448E-5 0.0 0.0 4.277547835817448E-5 8.555095671634896E-5 7 4.277547835817448E-5 0.0 0.0 4.277547835817448E-5 8.555095671634896E-5 8 8.555095671634896E-5 0.0 0.0 4.277547835817448E-5 1.2832643507452345E-4 9 8.555095671634896E-5 0.0 0.0 4.277547835817448E-5 1.2832643507452345E-4 10-11 8.555095671634896E-5 0.0 0.0 8.555095671634896E-5 1.2832643507452345E-4 12-13 8.555095671634896E-5 0.0 0.0 8.555095671634896E-5 1.9248965261178515E-4 14-15 1.2832643507452345E-4 0.0 0.0 8.555095671634896E-5 3.4220382686539583E-4 16-17 1.9248965261178515E-4 0.0 0.0 1.9248965261178515E-4 3.4220382686539583E-4 18-19 3.208160876863086E-4 0.0 0.0 2.138773917908724E-4 3.4220382686539583E-4 20-21 4.277547835817448E-4 0.0 0.0 2.566528701490469E-4 3.849793052235703E-4 22-23 4.277547835817448E-4 0.0 0.0 4.063670444026576E-4 4.063670444026576E-4 24-25 5.560812186562683E-4 0.0 0.0 9.83836002238013E-4 4.277547835817448E-4 26-27 5.560812186562683E-4 0.0 0.0 0.0021173861787296367 4.277547835817448E-4 28-29 6.844076537307917E-4 0.0 0.0 0.007549871930217796 4.277547835817448E-4 30-31 7.271831320889661E-4 0.0 0.0 0.02889483563094686 4.277547835817448E-4 32-33 7.485708712680534E-4 0.0 0.0 0.07665365721784867 5.133057402980938E-4 34-35 9.410605238798385E-4 0.0 0.0 0.15033441868980418 5.133057402980938E-4 36-37 0.0011121624373125365 0.0 0.0 0.2761584882803744 5.133057402980938E-4 38-39 0.0012404888723870598 0.0 0.0 0.46407116470783494 5.560812186562683E-4 40-41 0.001261876611566147 0.0 0.0 0.6776919036285582 5.560812186562683E-4 42-43 0.0013688153074615833 0.0 0.0 0.8942855382951748 5.774689578353556E-4 44-45 0.0016254681776106301 0.0 0.0 1.1468533502610159 5.988566970144427E-4 46-47 0.0017965700910433282 0.0 0.0 1.4436937823275677 5.988566970144427E-4 48-49 0.0018821210477596771 0.0 0.0 1.756339753647465 6.416321753726172E-4 50-51 0.0018821210477596771 0.0 0.0 2.0891757507524202 6.416321753726172E-4 52-53 0.0018821210477596771 0.0 0.0 2.4080669419126113 6.416321753726172E-4 54-55 0.0018821210477596771 0.0 0.0 2.7362404118765262 6.416321753726172E-4 56-57 0.0018821210477596771 0.0 0.0 3.1139906612575645 6.416321753726172E-4 58-59 0.0018821210477596771 0.0 0.0 3.4970664576941966 6.416321753726172E-4 60-61 0.0018821210477596771 0.0 0.0 3.896247221732681 6.630199145517045E-4 62-63 0.0019035087869387643 0.0 0.0 4.28287338287304 7.271831320889661E-4 64-65 0.0019248965261178515 0.0 0.0 4.669499544013401 7.271831320889661E-4 66-67 0.0020104474828342004 0.0 0.0 5.074818589196283 7.271831320889661E-4 68-69 0.002053222961192375 0.0 0.0 5.536130735550016 7.271831320889661E-4 70-71 0.002138773917908724 0.0 0.0 6.058205448911535 7.271831320889661E-4 72-73 0.0021815493962668983 0.0 0.0 6.58836472768275 8.768973063425769E-4 74-75 0.0021815493962668983 0.0 0.0 7.113262622615909 8.982850455216641E-4 76-77 0.0022029371354459855 0.0 0.0 7.672209798322175 8.982850455216641E-4 78-79 0.0022457126138041602 0.0 0.0 8.299234147835476 8.982850455216641E-4 80-81 0.0025237532231322944 0.0 0.0 8.963130959693522 9.196727847007513E-4 82-83 0.002566528701490469 0.0 0.0 9.61949928736053 9.83836002238013E-4 84-85 0.0026306919190277308 0.0 0.0 10.261623380734267 9.83836002238013E-4 86-87 0.0026948551365649923 0.0 0.0 10.903234168367705 0.0010266114805961876 88-89 0.0026948551365649923 0.0 0.0 11.588860923231703 0.0011121624373125365 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8745 0.0 32.073322 1 GGTATCA 5770 0.0 31.008574 1 TACATGG 10635 0.0 28.110777 2 GTACATG 11070 0.0 27.566372 1 ATCAACG 10830 0.0 25.538132 3 ACATGGG 11245 0.0 25.523779 3 TCAACGC 10980 0.0 25.189249 4 CAACGCA 11035 0.0 25.020714 5 TATCAAC 11220 0.0 24.826473 2 AACGCAG 11395 0.0 24.23024 6 CATGGGG 5560 0.0 21.075176 4 CATGGGA 5260 0.0 20.924318 4 ACGCAGA 13185 0.0 20.832792 7 CGCAGAG 13505 0.0 20.304033 8 GTATAGG 725 0.0 19.638103 1 GAGTACT 6340 0.0 19.567327 12-13 GTAAGGT 970 0.0 19.563084 4 AGTACTT 6660 0.0 19.26824 12-13 GGACCGA 570 0.0 19.142649 6 AGAGTAC 11610 0.0 19.123295 10-11 >>END_MODULE