FastQCFastQC Report
Fri 27 May 2016
SRR939773_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939773_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699035
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76150.44819559338094855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47900.2819247396316144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42140.24802314254856436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95900.039.425041
GGTATCA55500.036.070481
TATCAAC123400.031.2550832
ATCAACG127200.030.3959523
TCAACGC129150.029.7900834
CAACGCA130950.029.488415
TACATGG128700.029.009582
GTACATG125850.028.5725141
AACGCAG135350.028.5656816
ACATGGG132750.027.0881863
CATGGGG46250.024.9252664
ACGCAGA153700.024.7848857
CGCAGAG155200.024.453648
CATGGGA88450.023.7066234
GCAGAGT155200.023.414369
AGTACTT68000.022.35994712-13
CGTATCA1503.0647312E-522.1380441
ATGGGAG30000.021.9799165
ATGGGAT34100.021.5630285
GAGTACT66400.021.50552612-13
Produced by FastQC (version 0.11.5)