##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939773_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1699035 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.052425053044818 31.0 30.0 33.0 16.0 34.0 2 29.869721341820505 31.0 30.0 33.0 25.0 34.0 3 30.147652049545773 31.0 30.0 34.0 26.0 34.0 4 33.943599160700046 35.0 35.0 37.0 28.0 37.0 5 33.91326900269859 35.0 35.0 37.0 28.0 37.0 6 33.96126507105504 36.0 35.0 37.0 29.0 37.0 7 33.87766055437351 35.0 35.0 37.0 29.0 37.0 8 33.74915643291633 35.0 35.0 37.0 28.0 37.0 9 35.021612268140444 37.0 35.0 39.0 28.0 39.0 10-11 34.86341923503636 37.0 34.0 39.0 27.0 39.0 12-13 34.74143616817782 37.0 34.0 39.0 27.0 39.0 14-15 35.3915466720815 38.0 34.0 40.0 27.0 41.0 16-17 35.25575400153617 38.0 34.0 40.0 27.0 41.0 18-19 35.123938000100054 38.0 33.5 40.0 27.0 41.0 20-21 35.31469716633265 38.0 34.0 40.0 27.0 41.0 22-23 35.21303740064213 38.0 34.0 40.0 26.5 41.0 24-25 34.97583039784348 38.0 34.0 40.0 25.0 41.0 26-27 34.85876482827017 38.0 33.5 40.0 25.0 41.0 28-29 34.90369945292475 38.0 34.0 40.0 25.0 41.0 30-31 34.67723825583346 38.0 33.5 40.0 24.0 41.0 32-33 34.3208659621491 38.0 33.0 40.0 24.0 41.0 34-35 34.12759625316724 38.0 33.0 40.0 23.5 41.0 36-37 33.89952414164511 38.0 33.0 40.0 22.5 41.0 38-39 33.65167315564423 38.0 32.5 40.0 21.0 41.0 40-41 33.385324905019615 37.0 32.0 40.0 19.0 41.0 42-43 32.95419811834365 37.0 31.0 40.0 16.5 41.0 44-45 32.56175652649887 37.0 31.0 40.0 14.5 41.0 46-47 32.10805839785525 37.0 30.5 39.5 10.5 41.0 48-49 31.709141659824546 36.0 30.0 39.0 8.5 41.0 50-51 31.04168013019155 35.5 29.5 38.5 7.5 40.0 52-53 31.146026715164787 35.5 30.0 39.0 6.5 40.0 54-55 31.224740514468508 36.0 30.0 39.0 2.0 40.0 56-57 30.92225380877969 35.5 29.0 39.0 2.0 40.0 58-59 30.521442465870333 35.0 28.0 39.0 2.0 40.0 60-61 30.096732262725606 35.0 28.0 38.0 2.0 40.0 62-63 29.63587624739926 34.0 27.5 38.0 2.0 40.0 64-65 29.075101748933953 34.0 26.0 38.0 2.0 40.0 66-67 28.481392672899617 34.0 26.0 37.0 2.0 39.0 68-69 27.929105639377646 33.5 25.5 36.5 2.0 39.0 70-71 27.35421842398773 33.0 24.5 36.0 2.0 39.0 72-73 26.692403040549486 32.0 22.5 35.5 2.0 38.0 74-75 26.33110294961552 32.0 22.0 35.0 2.0 37.5 76-77 25.7553993884764 32.0 20.0 35.0 2.0 37.0 78-79 25.07757992036656 31.0 18.0 35.0 2.0 36.0 80-81 24.32693882115436 31.0 12.0 34.0 2.0 36.0 82-83 23.573178598439704 30.5 7.0 34.0 2.0 35.0 84-85 22.781298796081305 29.5 2.0 34.0 2.0 35.0 86-87 21.84585043863134 29.0 2.0 34.0 2.0 35.0 88-89 20.83360378096979 28.0 2.0 34.0 2.0 35.0 90-91 19.42452951234083 26.0 2.0 33.0 2.0 34.5 92-93 17.86807599608013 22.0 2.0 32.0 2.0 34.0 94-95 17.33385451153155 20.0 2.0 32.0 2.0 34.0 96-97 16.995860297168687 19.5 2.0 32.0 2.0 34.0 98-99 16.08826480914166 9.5 2.0 31.5 2.0 34.0 100-101 14.474464916849858 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.5133029382511225 1101 2 0.7483825944755793 1101 3 0.8804550645904179 1101 4 0.7587199996010341 1101 5 0.7883273622113904 1101 6 0.6915020070736944 1101 7 0.6384166432500038 1101 8 0.7911085755866907 1101 9 0.8745108344626047 1101 10-11 0.846717147066407 1101 12-13 0.8490940014146489 1101 14-15 1.1489806609435504 1101 16-17 0.9596638172464864 1101 18-19 1.0259266223387797 1101 20-21 0.7949086543079247 1101 22-23 0.904446681886121 1101 24-25 1.2455302070747294 1101 26-27 0.916694055713954 1101 28-29 0.8884392619416701 1101 30-31 0.9798171142533079 1101 32-33 1.0935688211233128 1101 34-35 1.0120694330009385 1101 36-37 0.8195058047285713 1101 38-39 0.8779114171121591 1101 40-41 0.7391937745636739 1101 42-43 0.9135040403119135 1101 44-45 0.7445849582823953 1101 46-47 0.5737786823783964 1101 48-49 0.4676409222693003 1101 50-51 0.29048438133812127 1101 52-53 0.528976588546989 1101 54-55 0.5151003268490051 1101 56-57 0.6407105485707199 1101 58-59 0.43791912030258473 1101 60-61 0.3234399632643097 1101 62-63 -0.11098331702590869 1101 64-65 0.07718568936207149 1101 66-67 0.17550641249545862 1101 68-69 0.0313254124619462 1101 70-71 -3.211628754939966E-5 1101 72-73 -0.1376831468697226 1101 74-75 -0.27512285915071644 1101 76-77 -0.4649340275482352 1101 78-79 -0.7720183269830976 1101 80-81 -0.7346426819337353 1101 82-83 -0.903800625885399 1101 84-85 -0.8251649609012865 1101 86-87 -1.0933890894221179 1101 88-89 -5.085395819594758 1101 90-91 -8.80284304914279 1101 92-93 -10.89185342624213 1101 94-95 -8.898324418462092 1101 96-97 -5.766724931367076 1101 98-99 -4.220054624237164 1101 100-101 -3.2096691351095874 1102 1 1.9472379604549381 1102 2 1.2347755674858654 1102 3 1.0501143568294395 1102 4 0.845156689967105 1102 5 1.0786868224170902 1102 6 0.8973690487892512 1102 7 0.9007466632679524 1102 8 1.0132646465468582 1102 9 1.2796614292487405 1102 10-11 1.2751076868607 1102 12-13 1.269886493534024 1102 14-15 1.5847506616338265 1102 16-17 1.3913468203987307 1102 18-19 1.5743184276354825 1102 20-21 1.50351450207949 1102 22-23 1.4350819065703249 1102 24-25 1.6065277105836273 1102 26-27 1.7506155600511661 1102 28-29 1.5010639426899246 1102 30-31 1.6057497032411376 1102 32-33 1.887406974870352 1102 34-35 1.8945200784071687 1102 36-37 1.7323385743381081 1102 38-39 1.651198818460493 1102 40-41 1.6474858352617687 1102 42-43 1.904227763880165 1102 44-45 1.8908691268473135 1102 46-47 1.8096827112778655 1102 48-49 1.5271447015196635 1102 50-51 1.887205435846763 1102 52-53 1.8366072804316644 1102 54-55 1.6105486885986195 1102 56-57 1.8093746957371444 1102 58-59 1.796479093611989 1102 60-61 1.6783242273171375 1102 62-63 1.6659992075363412 1102 64-65 1.52504627126309 1102 66-67 1.68396370754159 1102 68-69 1.5251790973748562 1102 70-71 1.573019729045047 1102 72-73 1.8045107165940735 1102 74-75 1.595519785979402 1102 76-77 1.568990784352092 1102 78-79 1.6039458995648452 1102 80-81 1.464627928498608 1102 82-83 1.2890317410642815 1102 84-85 1.4191136739667556 1102 86-87 1.4930799575395248 1102 88-89 1.1076910859067262 1102 90-91 -4.894814155421983 1102 92-93 -9.870037171438975 1102 94-95 -7.407627299205746 1102 96-97 -3.7085139778685363 1102 98-99 -2.246773262786668 1102 100-101 -1.451365713651267 1103 1 2.1048762159192798 1103 2 1.5048804625907586 1103 3 1.1360806248609236 1103 4 1.0626957933757737 1103 5 0.9327774781381848 1103 6 0.9963374113445695 1103 7 0.8166912259516437 1103 8 0.9388879363005813 1103 9 1.0305904519168934 1103 10-11 0.9079526498687045 1103 12-13 0.9895222321914972 1103 14-15 1.0577921129361485 1103 16-17 1.0196575193616084 1103 18-19 0.9482912241626238 1103 20-21 0.8012884370816877 1103 22-23 0.8727972709161236 1103 24-25 0.9217598817940527 1103 26-27 1.083051541943668 1103 28-29 0.9533322359908283 1103 30-31 0.9630845483477231 1103 32-33 1.1232520476719472 1103 34-35 1.003564114081641 1103 36-37 1.1435535670748393 1103 38-39 1.0667686049541985 1103 40-41 0.7476038633689299 1103 42-43 0.7258480216417311 1103 44-45 0.8141899183746304 1103 46-47 0.8862299563033744 1103 48-49 0.9335847288836057 1103 50-51 0.8303756412452294 1103 52-53 0.6864624373520414 1103 54-55 0.6853635174895345 1103 56-57 0.6929575983417884 1103 58-59 0.5803757525086475 1103 60-61 0.7362656819977254 1103 62-63 0.6463144840472701 1103 64-65 0.6620401650504597 1103 66-67 0.6013799265882476 1103 68-69 0.6589794757404519 1103 70-71 0.38144575350368015 1103 72-73 0.5934473234654654 1103 74-75 0.3026242756925903 1103 76-77 0.17380037693342132 1103 78-79 -0.023343930170636185 1103 80-81 -0.21044217048453362 1103 82-83 -0.27495047237445647 1103 84-85 -0.2906332518127819 1103 86-87 -0.4437940078670479 1103 88-89 -1.1176813677266004 1103 90-91 -6.498576486086488 1103 92-93 -9.809609525413503 1103 94-95 -8.348986966108892 1103 96-97 -5.146844819731987 1103 98-99 -3.6492396068290986 1103 100-101 -2.7279399847907566 1104 1 1.9787589222856319 1104 2 1.2837833443697484 1104 3 1.0599807195719073 1104 4 0.8631966023654272 1104 5 0.7915119766139256 1104 6 0.8674570974171232 1104 7 0.9408378405272728 1104 8 0.9066677036667343 1104 9 0.8991919110579829 1104 10-11 0.9983997893010255 1104 12-13 0.7811300127974548 1104 14-15 0.972589224251152 1104 16-17 0.9014143094485263 1104 18-19 0.9304768034288173 1104 20-21 0.9590935223708996 1104 22-23 0.9404536550283566 1104 24-25 0.942347188549121 1104 26-27 0.9852852659598099 1104 28-29 0.8612560183134264 1104 30-31 1.0670859640796095 1104 32-33 1.1059614354048932 1104 34-35 0.9994242082006792 1104 36-37 0.9067122893644708 1104 38-39 1.116409554135835 1104 40-41 1.1468880431816828 1104 42-43 1.2238733346319322 1104 44-45 1.1428049675552785 1104 46-47 1.0621241611750705 1104 48-49 0.7976260638151835 1104 50-51 0.809984107519238 1104 52-53 0.8138101721810216 1104 54-55 0.7895119942212148 1104 56-57 0.691615242800701 1104 58-59 0.8493725190164767 1104 60-61 0.8860178672563528 1104 62-63 0.8412193291060213 1104 64-65 0.7627775665165082 1104 66-67 0.6284332927744494 1104 68-69 0.5818017397275881 1104 70-71 0.4028892204189667 1104 72-73 0.4985054202679784 1104 74-75 0.5569035349737739 1104 76-77 0.5557332085945141 1104 78-79 0.25041613287136144 1104 80-81 0.19651538715597638 1104 82-83 0.1161133983450533 1104 84-85 0.07646707523608498 1104 86-87 0.20922928264903007 1104 88-89 -0.337426572983059 1104 90-91 -3.2596516900604016 1104 92-93 -7.999682630453902 1104 94-95 -5.941874639869017 1104 96-97 -3.4441278600532144 1104 98-99 -2.4543467856256775 1104 100-101 -1.6732974200962492 1105 1 1.920031937965895 1105 2 1.279301010596214 1105 3 1.0101510444604642 1105 4 0.8209762058809602 1105 5 0.8629564050357317 1105 6 0.9397623211353547 1105 7 0.9109667890541147 1105 8 0.9297169049802534 1105 9 1.0329013758849115 1105 10-11 0.9559074295708214 1105 12-13 0.8846782793163683 1105 14-15 0.9835499755274739 1105 16-17 0.889512438847369 1105 18-19 0.7545225919198373 1105 20-21 0.9102886110941668 1105 22-23 0.6895487447635773 1105 24-25 0.6971628354168686 1105 26-27 0.5083461680176242 1105 28-29 0.6629957608717518 1105 30-31 0.7735658362695332 1105 32-33 0.9928005330940977 1105 34-35 0.8286610160858316 1105 36-37 0.8433782160381256 1105 38-39 0.9550807986396492 1105 40-41 0.9193852539772323 1105 42-43 0.9505339864835562 1105 44-45 0.6960971142687029 1105 46-47 0.8754583926195849 1105 48-49 0.7272054505190901 1105 50-51 0.7483105911736168 1105 52-53 0.6381486373975527 1105 54-55 0.6996770000146668 1105 56-57 0.5115973944221075 1105 58-59 0.3510579005492893 1105 60-61 0.5069167563813686 1105 62-63 0.45095966112887353 1105 64-65 0.49847751768961857 1105 66-67 0.44826950132851806 1105 68-69 0.26271959670781087 1105 70-71 0.11157152054495256 1105 72-73 0.3718706365483335 1105 74-75 0.2667604949559603 1105 76-77 0.4469746322651815 1105 78-79 0.1286564484499202 1105 80-81 0.19809467139931058 1105 82-83 0.033349922882461414 1105 84-85 -0.0913268158369398 1105 86-87 0.2785585643624735 1105 88-89 0.21618165194445993 1105 90-91 -3.118376429102181 1105 92-93 -7.908815282740671 1105 94-95 -5.4333681987674005 1105 96-97 -2.723421305865486 1105 98-99 -1.7052912254319903 1105 100-101 -1.0679799694164664 1106 1 1.8340796997063151 1106 2 1.1365058653004994 1106 3 0.6872657763603769 1106 4 0.7531892531201478 1106 5 0.8175741900695215 1106 6 0.8437595893063019 1106 7 0.853068939385409 1106 8 -0.14467229602863796 1106 9 -0.38724428886180107 1106 10-11 -0.8207970359265531 1106 12-13 -0.2501536236446569 1106 14-15 0.5926002417725655 1106 16-17 0.6983301650326794 1106 18-19 0.8969688779214735 1106 20-21 0.7590012760150557 1106 22-23 0.7981738848731439 1106 24-25 0.6055638986258387 1106 26-27 0.7132594255564797 1106 28-29 0.5008145685700782 1106 30-31 0.4913706502325681 1106 32-33 0.46889309250104816 1106 34-35 0.5990166360663665 1106 36-37 0.7919906023636258 1106 38-39 0.6640027397734514 1106 40-41 0.6034683149641253 1106 42-43 0.8374405935538434 1106 44-45 0.8297855066522501 1106 46-47 0.7548942935376459 1106 48-49 0.5912142915681677 1106 50-51 0.5548147258205098 1106 52-53 0.5731323851796652 1106 54-55 0.5542134993568055 1106 56-57 0.6693437929050354 1106 58-59 0.3955485315368499 1106 60-61 0.28538667624706093 1106 62-63 0.4947373201599987 1106 64-65 0.506036791786741 1106 66-67 0.6525012777357908 1106 68-69 0.5480215494341749 1106 70-71 0.375759367251554 1106 72-73 0.3140195310727698 1106 74-75 0.07499613292563012 1106 76-77 -0.12966911498552136 1106 78-79 -0.13591125633146817 1106 80-81 -0.09210115232686178 1106 82-83 -0.1490680607403405 1106 84-85 -0.21486433764679802 1106 86-87 0.12917248783386626 1106 88-89 -0.16072022166121158 1106 90-91 -3.6995140154682282 1106 92-93 -8.531609325857515 1106 94-95 -6.049729256819752 1106 96-97 -3.28552711700517 1106 98-99 -2.2645038170383067 1106 100-101 -1.7674851698309642 1107 1 1.873772536989513 1107 2 0.9818035395138374 1107 3 0.8100308292459104 1107 4 0.92476689457731 1107 5 0.6772590834893535 1107 6 0.6442254331456354 1107 7 0.5946795822008752 1107 8 0.579665837948049 1107 9 0.661000460587772 1107 10-11 0.6449649129679287 1107 12-13 0.5946566308041525 1107 14-15 0.8270972039803652 1107 16-17 0.9335185375704569 1107 18-19 0.7859847705518206 1107 20-21 0.6670942094091927 1107 22-23 0.5608686198570396 1107 24-25 0.4348591014150145 1107 26-27 0.6589650809006926 1107 28-29 0.5551221529981376 1107 30-31 0.536167664909101 1107 32-33 0.8156898031531767 1107 34-35 0.929099850486935 1107 36-37 0.4969785639780966 1107 38-39 0.5914620274737032 1107 40-41 0.7197517637777011 1107 42-43 0.8446204730228786 1107 44-45 0.5861148533430409 1107 46-47 0.7282465548417107 1107 48-49 0.9767966699216295 1107 50-51 1.0772355446268698 1107 52-53 1.0158257471501315 1107 54-55 1.1102427445426741 1107 56-57 1.0261546750171284 1107 58-59 0.8071563792892746 1107 60-61 0.5545674285603397 1107 62-63 0.7197320487691847 1107 64-65 0.7157562557230754 1107 66-67 0.9656621017399871 1107 68-69 0.9679724151681768 1107 70-71 0.775116012391333 1107 72-73 0.6848817344650904 1107 74-75 0.6802905707501878 1107 76-77 0.708849305460614 1107 78-79 0.7048054566744035 1107 80-81 0.6504088142794942 1107 82-83 0.4478539373864763 1107 84-85 0.6213944949975776 1107 86-87 0.7947294654390369 1107 88-89 0.5956346238502626 1107 90-91 -2.479389071246896 1107 92-93 -3.6666329367847403 1107 94-95 -2.059398213476662 1107 96-97 -0.6181504062549656 1107 98-99 -0.1744037341671394 1107 100-101 -0.08322922051477377 1108 1 1.7460728916428074 1108 2 0.9743272849787594 1108 3 -0.37503840288214363 1108 4 -0.01977040290116605 1108 5 0.5976292273889072 1108 6 0.7132935963314466 1108 7 0.5919773324397966 1108 8 0.4841359188887182 1108 9 0.8867538157528898 1108 10-11 0.6979876189318119 1108 12-13 0.7412104244167708 1108 14-15 0.6169807526874465 1108 16-17 0.8056789332602605 1108 18-19 0.877983556668255 1108 20-21 0.6932552120407891 1108 22-23 0.6841575196165266 1108 24-25 0.572419738975654 1108 26-27 0.8174253511256708 1108 28-29 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2.102108316853272 2311 90-91 2.583497587202359 2311 92-93 3.732611242174176 2311 94-95 3.4383252932801263 2311 96-97 2.568977157734725 2311 98-99 2.3263945284377794 2311 100-101 2.128375838158428 2312 1 1.2760838787842488 2312 2 0.6117563665146335 2312 3 -0.2549363478983899 2312 4 -0.6822223987588458 2312 5 -0.11112743773849587 2312 6 -0.16713903947344733 2312 7 0.14058907792586695 2312 8 0.10681546841871636 2312 9 -0.05140223853079817 2312 10-11 -0.1613135564101711 2312 12-13 0.009417078774937693 2312 14-15 -0.11239475137874422 2312 16-17 -0.3823954787125743 2312 18-19 -0.6240588280526538 2312 20-21 0.18885881899668533 2312 22-23 0.16851767436956067 2312 24-25 0.26272496249121247 2312 26-27 0.40412515521205705 2312 28-29 0.3413395572967204 2312 30-31 0.046470332038332174 2312 32-33 0.17152650437733286 2312 34-35 -0.03595127987784963 2312 36-37 0.11030444815268226 2312 38-39 0.13647777433447317 2312 40-41 -2.9518394452310304E-4 2312 42-43 0.014848167973816828 2312 44-45 0.00853847318363421 2312 46-47 0.05742968550085692 2312 48-49 0.333585992393612 2312 50-51 0.08164106120950976 2312 52-53 0.001780027938366402 2312 54-55 0.013887726047435933 2312 56-57 0.19804161000368836 2312 58-59 0.12052010999617835 2312 60-61 0.22039291099099145 2312 62-63 0.23939227101243077 2312 64-65 0.12463982807527785 2312 66-67 0.06613578413248788 2312 68-69 0.3976139093853135 2312 70-71 0.43972529950984196 2312 72-73 0.18952153910489855 2312 74-75 0.366857304549395 2312 76-77 0.5970649165544479 2312 78-79 0.48632148062585046 2312 80-81 0.6574230382678863 2312 82-83 0.5259938103602977 2312 84-85 1.034857798128776 2312 86-87 1.553574365243346 2312 88-89 1.9214280869130995 2312 90-91 2.477703023483574 2312 92-93 3.556958969463995 2312 94-95 3.0799830557939067 2312 96-97 2.164916724782646 2312 98-99 1.938331774140325 2312 100-101 1.9140231512763446 2313 1 -7.6004044342647035 2313 2 -3.1428498516322456 2313 3 -1.6008643762213204 2313 4 -0.9493357290033941 2313 5 -0.6273097297433452 2313 6 -0.16552898161921803 2313 7 -0.24222006033551224 2313 8 0.055089470991724454 2313 9 -0.027169932384296658 2313 10-11 -0.16483782778471578 2313 12-13 -0.32904778663721146 2313 14-15 -0.2918467319259932 2313 16-17 -0.5699493353128702 2313 18-19 -0.46963427791691004 2313 20-21 -0.2652186277574984 2313 22-23 -1.1780760297869861 2313 24-25 -0.3719298820441139 2313 26-27 -0.3526364274649083 2313 28-29 -0.477804201170386 2313 30-31 -0.07832552187009867 2313 32-33 -0.3369661931353818 2313 34-35 0.1408231403417517 2313 36-37 0.29601025987826546 2313 38-39 0.14034458194081623 2313 40-41 -0.08640645401934677 2313 42-43 0.06608182115436279 2313 44-45 -0.12446229277690435 2313 46-47 -1.0984091678474144 2313 48-49 -0.4489912667222562 2313 50-51 -0.3862170721138476 2313 52-53 -0.6698284231595686 2313 54-55 -0.5891050776652733 2313 56-57 -0.2082164031431013 2313 58-59 -0.2642324055128711 2313 60-61 -0.022989536627104457 2313 62-63 0.008949851765692074 2313 64-65 -0.3106614057154644 2313 66-67 -0.11505597782986499 2313 68-69 0.0011522949033775376 2313 70-71 0.42763253244438815 2313 72-73 0.23015679111801646 2313 74-75 0.2745860224569121 2313 76-77 0.1952817472166437 2313 78-79 0.16163592613535016 2313 80-81 0.23919510337893257 2313 82-83 0.3920754875057284 2313 84-85 0.6773253576090248 2313 86-87 1.3696292447751404 2313 88-89 1.5068892259712534 2313 90-91 2.2332336767620724 2313 92-93 3.2400637068638893 2313 94-95 2.8585588314114894 2313 96-97 1.9738491892936914 2313 98-99 1.6688963508866 2313 100-101 1.7179606396521212 2314 1 0.5748100271726244 2314 2 -0.14180797345823493 2314 3 -1.538266428454893 2314 4 -0.3125453980815678 2314 5 -0.20713722949373903 2314 6 -3.4534609193499577 2314 7 -2.608056440309465 2314 8 -2.1847410688554163 2314 9 -3.3501224384448562 2314 10-11 -2.5750670522010566 2314 12-13 -1.118783589890235 2314 14-15 -1.1696254476589942 2314 16-17 -2.115158995416593 2314 18-19 -3.322030709897831 2314 20-21 -3.0240022356904745 2314 22-23 -4.035273514920238 2314 24-25 -3.2827244971465532 2314 26-27 -1.8397056395862634 2314 28-29 -2.1246306374478223 2314 30-31 -1.9851646457837688 2314 32-33 -2.3924659076355255 2314 34-35 -1.531247877888731 2314 36-37 -1.9398311345726071 2314 38-39 -3.206383079237604 2314 40-41 -2.73160514846046 2314 42-43 -1.3577554820666755 2314 44-45 -1.8821863540769215 2314 46-47 -1.4677225883601004 2314 48-49 -2.004332245543665 2314 50-51 -2.925387344454915 2314 52-53 -1.9172684597753467 2314 54-55 -2.4863517708589313 2314 56-57 -1.47126315781178 2314 58-59 -0.6804021584573334 2314 60-61 -1.0579931376273066 2314 62-63 -0.8623216827203315 2314 64-65 -1.2842281291081683 2314 66-67 -0.8722849016019651 2314 68-69 -0.7549685640866137 2314 70-71 -0.6534020315485272 2314 72-73 -1.7481847631903342 2314 74-75 -1.5621422725424061 2314 76-77 -0.7251473663651851 2314 78-79 -1.8777156582486505 2314 80-81 -0.8209929226783963 2314 82-83 -0.44391059199223903 2314 84-85 -1.5293081110516162 2314 86-87 -0.10998233518894196 2314 88-89 1.2337596707340417 2314 90-91 2.1125348247070868 2314 92-93 3.2547492812122023 2314 94-95 2.9231193803789246 2314 96-97 1.8492141972192577 2314 98-99 1.722278654831209 2314 100-101 1.7823341273161706 2315 1 -2.6751719475706537 2315 2 -1.7397216130438942 2315 3 -2.766499113600986 2315 4 -4.008449080604429 2315 5 -4.164443333364915 2315 6 -3.047295361743462 2315 7 -5.943552903353535 2315 8 -2.773122834001306 2315 9 -2.3895667442588433 2315 10-11 -3.785548049129794 2315 12-13 -2.91113025874861 2315 14-15 -1.918219403456611 2315 16-17 -2.4159078076040927 2315 18-19 -2.1385015515601182 2315 20-21 -1.3473943308662015 2315 22-23 -0.7197724765764448 2315 24-25 -2.176320442398435 2315 26-27 -3.991668196899898 2315 28-29 -3.2803805756575457 2315 30-31 -3.3746299143037213 2315 32-33 -3.949258966710005 2315 34-35 -3.652771933372698 2315 36-37 -2.997294169444281 2315 38-39 -1.7668369715210446 2315 40-41 -1.4797607122118634 2315 42-43 -2.613652411423395 2315 44-45 -6.135411243114035 2315 46-47 -2.758272517431088 2315 48-49 -1.634839406540511 2315 50-51 -2.887035977458016 2315 52-53 -2.9924379342775467 2315 54-55 -1.5662296204443251 2315 56-57 -0.9790175787470652 2315 58-59 -2.4022942555616886 2315 60-61 -2.4594121926528523 2315 62-63 -4.188638765233879 2315 64-65 -3.7113421505028867 2315 66-67 -3.1783190454438106 2315 68-69 -1.4679486182128265 2315 70-71 -2.9890840879766323 2315 72-73 -1.9182326614157326 2315 74-75 -1.2328522836958165 2315 76-77 -3.2035330807483895 2315 78-79 -1.336576978302734 2315 80-81 -1.008117192507143 2315 82-83 -0.5771615976235367 2315 84-85 -2.2796016334419527 2315 86-87 -1.69709432113903 2315 88-89 -0.956901555024956 2315 90-91 0.05668471651103957 2315 92-93 0.7123756857357542 2315 94-95 0.2931801262358569 2315 96-97 0.04857635533651106 2315 98-99 -0.5527173321383856 2315 100-101 -0.8717045241750583 2316 1 -7.510058973045805 2316 2 -6.053192297049748 2316 3 -3.466034675487009 2316 4 -4.015469448234619 2316 5 -2.0481350073022746 2316 6 -2.9504661056668446 2316 7 -3.2784798120111525 2316 8 -2.5869465923821835 2316 9 -7.21358853964513 2316 10-11 -7.155846239298995 2316 12-13 -6.032506840704222 2316 14-15 -4.843088762846136 2316 16-17 -4.998688753707388 2316 18-19 -4.091875637879692 2316 20-21 -2.9086576816842395 2316 22-23 -3.489048111414675 2316 24-25 -3.2965561798322938 2316 26-27 -4.4443004251083735 2316 28-29 -4.378743015469492 2316 30-31 -7.217377636788498 2316 32-33 -5.1638983516309445 2316 34-35 -5.562950059704072 2316 36-37 -4.371857077589908 2316 38-39 -6.703428431078571 2316 40-41 -3.994127314715044 2316 42-43 -4.966738689568089 2316 44-45 -3.389820962738355 2316 46-47 -4.297945926725909 2316 48-49 -5.4308567152233564 2316 50-51 -4.712379792132925 2316 52-53 -6.1652795893863335 2316 54-55 -5.189872587431559 2316 56-57 -4.2536343137304335 2316 58-59 -3.340267713767517 2316 60-61 -3.4126711124184013 2316 62-63 -4.50870486001487 2316 64-65 -2.992657714281041 2316 66-67 -4.107918581191203 2316 68-69 -3.060293489637484 2316 70-71 -2.527495148170555 2316 72-73 -2.2384899790522006 2316 74-75 -2.3968196215335773 2316 76-77 -3.2051500500840966 2316 78-79 -5.021890784747487 2316 80-81 -5.782400021776823 2316 82-83 -3.8016801086761234 2316 84-85 -2.759667771507022 2316 86-87 -2.4910795971337443 2316 88-89 -1.5120024903937939 2316 90-91 -1.4981688812921057 2316 92-93 -1.4285418005972996 2316 94-95 -1.7920149890945574 2316 96-97 -3.561582385913148 2316 98-99 -4.835407010679935 2316 100-101 -4.25514963347662 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13506.0 3 4233.0 4 3956.0 5 4469.0 6 5137.0 7 5849.0 8 7219.0 9 8660.0 10 10382.0 11 12508.0 12 14749.0 13 17133.0 14 19066.0 15 21957.0 16 24955.0 17 26750.0 18 24079.0 19 22787.0 20 24720.0 21 27188.0 22 30158.0 23 34100.0 24 38554.0 25 43681.0 26 49787.0 27 56466.0 28 64421.0 29 73969.0 30 83341.0 31 94445.0 32 106102.0 33 117081.0 34 133471.0 35 153209.0 36 160917.0 37 124600.0 38 34955.0 39 475.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.10885419766845 19.492919364619866 13.114424647176378 22.283801790535303 2 16.15653544068019 20.601609737384997 39.036868159809366 24.204986662125442 3 19.802609751771275 25.816954370300003 28.555702813824624 25.824733064104098 4 12.926391276596874 15.892970699016779 35.91213653714795 35.26850148723839 5 14.048333205654783 38.24563781327896 32.166921041621244 15.53910793944501 6 32.31148422139553 34.770452305849844 17.76843430414303 15.149629168611586 7 29.814483361985005 32.885636196849966 19.65398682730469 17.645893613860338 8 28.12412685725199 32.64867152620342 19.674875534790722 19.552326081753865 9 28.835772501510665 15.820960634331108 19.1781992896199 36.16506757453833 10-11 26.070098318502193 26.25095460937429 26.128959141981916 21.549987930141604 12-13 27.2758539794215 23.83759655013062 26.793339101977686 22.093210368470192 14-15 23.97707096929356 24.636977645108733 25.671961246177315 25.71399013942039 16-17 23.081937837821904 28.225672401902706 25.77682092270694 22.915568837568447 18-19 22.941252067738098 26.18590601415553 28.99611389036596 21.87672802774041 20-21 24.166930735388405 25.306115831595395 28.52525848029065 22.001694952725558 22-23 23.57472144916613 25.570267702493027 28.42480557614745 22.430205272193394 24-25 22.879909489637527 25.74003458434927 28.897810414885367 22.48224551112784 26-27 22.796274041652435 26.021620894534426 28.68688817346272 22.49521689035042 28-29 22.568044492359697 26.037793728771813 28.90077265802324 22.493389120845247 30-31 22.57816578431228 26.15708365899338 28.697910368524283 22.56684018817006 32-33 22.309199210040134 26.20558540320278 28.42136029767424 23.063855089082843 34-35 22.87950427568708 26.0590276907397 28.50998712147776 22.55148091209546 36-37 23.008748027262488 25.799793723398828 28.211200813545005 22.98025743579368 38-39 22.74676681396813 25.851804995864057 28.50364419987585 22.897783990291963 40-41 22.68215538558859 25.854600093486745 28.741099221287847 22.722145299636818 42-43 22.72238160913846 25.814635228076632 28.313220503320586 23.149762659464322 44-45 22.485672961668264 26.04915792251678 28.29515984212614 23.17000927368882 46-47 22.62176013608564 26.124349196031858 27.853289158978722 23.400601508903783 48-49 22.637860553377596 26.28065633046881 27.59739455119203 23.484088564961564 50-51 22.597854951834808 26.587079260807183 27.40516910996108 23.409896677396922 52-53 22.861400665544934 26.355850868132237 27.365940255555326 23.4168082107675 54-55 22.16736313288245 26.18657899177812 27.82281238604487 23.82324548929456 56-57 22.488495383110674 26.607859064682465 27.349516779544935 23.55412877266193 58-59 22.532602790151117 25.997344230130544 27.374044126365565 24.096008853352778 60-61 22.533845308246484 26.015860528314704 27.68689521931335 23.76339894412546 62-63 22.56074853983252 25.94930419986301 27.50286519814021 23.98708206216426 64-65 22.30728767691993 25.97312226485815 27.60142544344607 24.118164614775857 66-67 22.368826122957376 26.259899080685912 27.33452582312463 24.036748973232083 68-69 22.550862588813253 25.967189041192835 27.4293069359483 24.052641434045608 70-71 22.255158576158667 26.273587075633166 27.261893949134368 24.209360399073798 72-73 22.467067041141384 26.207664735590903 26.957725281438634 24.367542941829086 74-75 22.449020529253737 26.36492707989485 26.881012453601727 24.30503993724968 76-77 22.508002061698114 26.14715886611324 27.207894875818685 24.13694419636996 78-79 22.562699899307248 26.326685290744795 26.919011222536295 24.191603587411663 80-81 22.418986289987537 26.701240699475015 26.83363744948446 24.04613556105299 82-83 22.792344001220393 26.480866051888402 26.52338495303004 24.20340499386117 84-85 22.609479808481687 26.228368864202945 26.50031873504224 24.661832592273132 86-87 22.692092797135917 26.087232281348477 26.88172265052749 24.33895227098812 88-89 22.767469152301164 25.985848970327325 26.647083916715196 24.599597960656315 90-91 22.504417261398427 26.19874927511987 26.974851314597093 24.321982148884615 92-93 22.940191787674852 25.84213852581911 26.96255270904901 24.255116977457032 94-95 22.801210502398654 25.83455305810122 26.791829079281765 24.57240736021836 96-97 23.426238172937396 25.63274655815915 26.16362713998231 24.777388128921142 98-99 23.343847556070525 25.686388829437856 25.874877305367566 25.094886309124053 100-101 23.786162812636466 25.67709174527821 25.963409128555238 24.573336313530085 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2419.0 1 1471.0 2 571.0 3 625.0 4 577.5 5 574.5 6 883.0 7 1607.0 8 2369.0 9 2539.0 10 2221.0 11 1956.5 12 1844.5 13 1899.5 14 2019.0 15 2022.5 16 2120.5 17 2302.0 18 2458.5 19 2637.0 20 2890.5 21 3214.0 22 3572.0 23 4066.5 24 4654.5 25 5366.0 26 6293.0 27 7464.5 28 8782.0 29 10265.5 30 11964.0 31 14323.0 32 17527.0 33 20491.5 34 23288.0 35 26513.0 36 30663.0 37 35274.5 38 38690.5 39 41020.0 40 43722.0 41 47861.0 42 52069.0 43 55702.5 44 59363.0 45 62547.5 46 64536.5 47 67017.5 48 72127.0 49 73607.0 50 71132.5 51 68495.5 52 66202.0 53 65618.0 54 63991.5 55 59753.5 56 55154.0 57 50904.5 58 45909.0 59 40738.5 60 35337.0 61 29000.0 62 23081.0 63 17873.5 64 13454.0 65 10604.5 66 8338.0 67 6451.5 68 4658.0 69 3166.0 70 2316.5 71 1841.0 72 1501.0 73 1214.5 74 1001.5 75 796.5 76 551.0 77 345.0 78 224.0 79 173.5 80 128.0 81 83.5 82 56.0 83 37.0 84 27.5 85 20.5 86 14.5 87 13.5 88 11.5 89 9.5 90 7.0 91 4.5 92 2.5 93 2.5 94 2.5 95 1.0 96 0.0 97 0.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13089783318177672 2 0.11700759548802704 3 0.12312871718357773 4 0.13336982463574912 5 0.12153957982030976 6 0.13578295915034122 7 0.1647405733254465 8 0.15167433278302095 9 0.16244515268961499 10-11 0.15658888722127562 12-13 0.14755434702640027 14-15 0.15202747441930273 16-17 0.1474366331476397 18-19 0.1610914430838682 20-21 0.16612371140088344 22-23 0.19917188286291926 24-25 0.22127266360022013 26-27 0.23589861303622348 28-29 0.2302777753254053 30-31 0.22130209206991028 32-33 0.22124323513052999 34-35 0.21050184369362607 36-37 0.2200366678732339 38-39 0.2079121383608931 40-41 0.21232640881441522 42-43 0.2045572928162163 44-45 0.19878931275694733 46-47 0.21376840382923246 48-49 0.2518488436082835 50-51 0.25276112616867813 52-53 0.2550565468045096 54-55 0.2524374130020865 56-57 0.22636378885661565 58-59 0.2270700721291792 60-61 0.19887759816601774 62-63 0.1914910522737907 64-65 0.19316847504612913 66-67 0.20146730349875075 68-69 0.20084930563525769 70-71 0.20652900028545618 72-73 0.23769374968732251 74-75 0.23916517317182992 76-77 0.2539971218956643 78-79 0.2516428443204525 80-81 0.26526822578699083 82-83 0.26453251404473715 84-85 0.23857660377802695 86-87 0.2420197347317742 88-89 0.24578657885211314 90-91 0.43412878486905804 92-93 0.5843022656978814 94-95 0.3348076996648097 96-97 0.2810124570712198 98-99 0.31179463636711424 100-101 0.3316294249382738 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1699035.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.95842858677319 #Duplication Level Percentage of deduplicated Percentage of total 1 75.922163656971 39.44796318524034 2 12.187508715846851 12.664876025260089 3 4.825677222183334 7.522038159948923 4 2.3320553738590033 4.846797304122146 5 1.2724294519111898 3.305671740441725 6 0.7709550653275697 2.403456822326015 7 0.540604368636531 1.9662267437049161 8 0.3822699163455538 1.588971531944977 9 0.2527266265111076 1.1818150537998133 >10 1.3372615263433891 12.565147880407402 >50 0.10834682405745182 3.906301494392369 >100 0.06355389066543536 6.055471726376655 >500 0.0036281105682499123 1.2419221284198831 >1k 7.022149486935314E-4 0.8402722406358489 >5k 1.1703582478225524E-4 0.4630679629788674 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7615 0.44819559338094855 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4790 0.2819247396316144 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4214 0.24802314254856436 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 5.8856939380295285E-5 0.0 0.0 0.0 0.0 9 5.8856939380295285E-5 0.0 0.0 0.0 0.0 10-11 5.8856939380295285E-5 0.0 0.0 0.0 0.0 12-13 5.8856939380295285E-5 0.0 0.0 0.0 0.0 14-15 5.8856939380295285E-5 0.0 0.0 8.828540907044292E-5 0.0 16-17 5.8856939380295285E-5 0.0 0.0 1.7657081814088585E-4 0.0 18-19 5.8856939380295285E-5 0.0 0.0 3.2371316659162406E-4 5.8856939380295285E-5 20-21 5.8856939380295285E-5 0.0 0.0 6.179978634931005E-4 5.8856939380295285E-5 22-23 5.8856939380295285E-5 0.0 0.0 0.0019422789995497444 5.8856939380295285E-5 24-25 5.8856939380295285E-5 0.0 0.0 0.004237699635381261 5.8856939380295285E-5 26-27 5.8856939380295285E-5 0.0 0.0 0.008269399982931488 5.8856939380295285E-5 28-29 5.8856939380295285E-5 0.0 0.0 0.01824565120789154 5.8856939380295285E-5 30-31 5.8856939380295285E-5 0.0 0.0 0.0523826760484628 5.8856939380295285E-5 32-33 5.8856939380295285E-5 0.0 0.0 0.12457071219839497 5.8856939380295285E-5 34-35 5.8856939380295285E-5 0.0 0.0 0.23698746641475896 5.8856939380295285E-5 36-37 5.8856939380295285E-5 0.0 0.0 0.4219453984173369 5.8856939380295285E-5 38-39 5.8856939380295285E-5 0.0 0.0 0.7128163928347562 5.8856939380295285E-5 40-41 5.8856939380295285E-5 0.0 0.0 1.0524503615287502 5.8856939380295285E-5 42-43 5.8856939380295285E-5 0.0 0.0 1.3727792541060073 5.8856939380295285E-5 44-45 5.8856939380295285E-5 0.0 0.0 1.7434896867927971 5.8856939380295285E-5 46-47 5.8856939380295285E-5 0.0 0.0 2.1679659336034867 5.8856939380295285E-5 48-49 5.8856939380295285E-5 0.0 0.0 2.630846333359819 5.8856939380295285E-5 50-51 5.8856939380295285E-5 0.0 0.0 3.1400471444084435 5.8856939380295285E-5 52-53 5.8856939380295285E-5 0.0 0.0 3.626882318492556 5.8856939380295285E-5 54-55 5.8856939380295285E-5 0.0 0.0 4.12410574237729 5.8856939380295285E-5 56-57 5.8856939380295285E-5 0.0 0.0 4.663441306388626 5.8856939380295285E-5 58-59 5.8856939380295285E-5 0.0 0.0 5.248685283116592 5.8856939380295285E-5 60-61 5.8856939380295285E-5 0.0 0.0 5.902232737995391 5.8856939380295285E-5 62-63 5.8856939380295285E-5 0.0 0.0 6.522879163760605 5.8856939380295285E-5 64-65 5.8856939380295285E-5 0.0 0.0 7.130371063574323 5.8856939380295285E-5 66-67 5.8856939380295285E-5 0.0 0.0 7.789186214527659 5.8856939380295285E-5 68-69 5.8856939380295285E-5 0.0 0.0 8.509712866421232 5.8856939380295285E-5 70-71 5.8856939380295285E-5 0.0 0.0 9.278649350955101 5.8856939380295285E-5 72-73 5.8856939380295285E-5 0.0 0.0 10.031429605629079 5.8856939380295285E-5 74-75 5.8856939380295285E-5 0.0 0.0 10.758695377081697 5.8856939380295285E-5 76-77 5.8856939380295285E-5 0.0 0.0 11.500233956334036 5.8856939380295285E-5 78-79 5.8856939380295285E-5 0.0 0.0 12.295155779604304 5.8856939380295285E-5 80-81 5.8856939380295285E-5 0.0 0.0 13.115003516702128 5.8856939380295285E-5 82-83 5.8856939380295285E-5 0.0 0.0 13.890384836098137 5.8856939380295285E-5 84-85 5.8856939380295285E-5 0.0 0.0 14.620740596868222 5.8856939380295285E-5 86-87 5.8856939380295285E-5 5.8856939380295285E-5 0.0 15.353097493577236 5.8856939380295285E-5 88-89 5.8856939380295285E-5 1.1771387876059057E-4 0.0 16.107937741129525 5.8856939380295285E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9590 0.0 39.42504 1 GGTATCA 5550 0.0 36.07048 1 TATCAAC 12340 0.0 31.255083 2 ATCAACG 12720 0.0 30.395952 3 TCAACGC 12915 0.0 29.790083 4 CAACGCA 13095 0.0 29.48841 5 TACATGG 12870 0.0 29.00958 2 GTACATG 12585 0.0 28.572514 1 AACGCAG 13535 0.0 28.565681 6 ACATGGG 13275 0.0 27.088186 3 CATGGGG 4625 0.0 24.925266 4 ACGCAGA 15370 0.0 24.784885 7 CGCAGAG 15520 0.0 24.45364 8 CATGGGA 8845 0.0 23.706623 4 GCAGAGT 15520 0.0 23.41436 9 AGTACTT 6800 0.0 22.359947 12-13 CGTATCA 150 3.0647312E-5 22.138044 1 ATGGGAG 3000 0.0 21.979916 5 ATGGGAT 3410 0.0 21.563028 5 GAGTACT 6640 0.0 21.505526 12-13 >>END_MODULE