FastQCFastQC Report
Fri 27 May 2016
SRR939773_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939773_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699035
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84130.49516343100642424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58140.34219424555703676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50260.2958149773253641No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91050.039.057011
GGTATCA52150.037.260661
TATCAAC118650.029.9231222
ATCAACG121000.029.3411083
TCAACGC122600.028.918034
CAACGCA125500.028.174675
AACGCAG128500.027.5365586
GTACATG134050.027.0562211
TACATGG132800.026.8412912
ACATGGG135500.025.0878163
ATGGGAG30800.024.0320195
ACGCAGA148500.023.7962047
GTCGGGA7000.023.574892
CGCAGAG149850.023.550388
CATGGGA91200.023.2109454
GCAGAGT156750.022.183079
CATGGGG42950.021.8439524
AGTACTT68400.021.56061212-13
CGGGAGT8550.020.4021974
GAGTACT68100.020.3756312-13
Produced by FastQC (version 0.11.5)